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公开(公告)号:JP2001166245A
公开(公告)日:2001-06-22
申请号:JP2000356044
申请日:2000-11-22
Applicant: LEICA MICROSYSTEMS
Inventor: STORZ RAFAEL , ENGELHARDT JOHANN , BIRK HOLGER , BRADL JOACHIM
Abstract: PROBLEM TO BE SOLVED: To provide a beam deflecting device which gives the possibility of flexible and diverse uses by actualizing the maximum changeability of a frequency range and the highest reachable vibration frequency. SOLUTION: This beam deflecting device deflects a light beam by a mirror device (4) which can be rotated alternately by a rotary driving mechanism (1), and the rotary driving mechanism (1) is equipped with 1st and 2nd driving devices (2, 3) which rotate the mirror device (4) on a rotary shaft (5) together or independently of each other and are independent of each other; and the 2nd driving device (3) is fitted to the shaft (6) of the 1st driving device (2) so that the 2nd driving device (3) can be rotated by the 1st driving device (2), and the mirror device (4) is fitted to the shaft (7) of the 2nd driving device (3).
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公开(公告)号:JP2009139969A
公开(公告)日:2009-06-25
申请号:JP2009013039
申请日:2009-01-23
Inventor: ENGELHARDT JOHANN , BRADL JOACHIM , ULRICH HEINRICH
IPC: G01N21/64 , G02B21/06 , G01J3/36 , G02B5/04 , G02B5/32 , G02B21/00 , G02B21/18 , G02F1/11 , G02F1/33
CPC classification number: G02B21/0076 , G02B21/0024
Abstract: PROBLEM TO BE SOLVED: To design and develop an optical arrangement in a beam path of a light source suitable for fluorescence excitation, preferably in a beam path of a confocal laser scanning microscope such that injection of excitation light of various excitation wavelengths is possible without having to switch or make special adjustment to the optical elements used when switching the wavelength of the excitation light.
SOLUTION: The optical arrangement comprises at least one spectrally selective element (4) for injecting excitation light (3) of at least one light source (2) in the microscope and for separating and extracting the excitation light (3) scattered and reflected on an object (10) or for extracting the excitation light from light (13) coming from the object (10) via a detection beam path (12). The optical arrangement in the beam path of the light source suitable for fluorescence excitation, preferably in the beam path of the confocal laser scanning microscope is characterized in that excitation light (3, 9) of different wavelengths can be separated and extracted by the spectrally selective element (4) so that configuration can be changed with the simplest structure. Alternatively, the optical arrangement like this is characterized in that the spectrally selective element (4) can be adjusted to the excitation wavelength to be separated and extracted.
COPYRIGHT: (C)2009,JPO&INPITAbstract translation: 要解决的问题:为了设计和开发适合于荧光激发的光源的光束路径中的光学布置,优选地在共聚焦激光扫描显微镜的光束路径中,使得各种激发波长的激发光的注入是 可以在切换激发光的波长时不必切换或对所使用的光学元件进行特殊调整。 解决方案:光学装置包括至少一个光谱选择性元件(4),用于在显微镜中注入至少一个光源(2)的激发光(3),并用于分离和提取散射的激发光(3) 在物体(10)上反射或者经由检测光束路径(12)从来自物体(10)的光(13)提取激发光。 适用于荧光激发的光源的光路中的光学布置优选在共聚焦激光扫描显微镜的光束路径中的特征在于可以通过光谱选择性分离和提取不同波长的激发光(3,9) 元件(4),从而可以以最简单的结构改变配置。 或者,这样的光学布置的特征在于,可以将光谱选择性元件(4)调整到激发波长以被分离和提取。 版权所有(C)2009,JPO&INPIT
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公开(公告)号:DE19956439A1
公开(公告)日:2001-05-31
申请号:DE19956439
申请日:1999-11-24
Applicant: LEICA MICROSYSTEMS
Inventor: STORZ RAFAEL , ENGELHARDT JOHANN , BIRK HOLGER , BRADL JOACHIM
Abstract: A beam of light is deflected by an arrangement (4) of mirrors rotated alternately by a rotating driving mechanism (1). It is possible to adjust almost any offset angle at all to a zero pint position. The rotating driving mechanism has two operating devices (2,3) working jointly but separate from each other to turn the arrangement of mirrors around an axis (5) of rotation.
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公开(公告)号:DE19914049A1
公开(公告)日:2000-10-05
申请号:DE19914049
申请日:1999-03-27
Applicant: LEICA MICROSYSTEMS
Inventor: ENGELHARDT JOHANN , BRADL JOACHIM
Abstract: The objective (2) and beam divider and combiner (5) consists of a modular unit (8) coupled by interface (9) to the illuminating and scanning beam path (4) of the microscope (1). The unit (8) and the interface (9) are placed in the stand and so coupled to the microscope instead of the conventional objective turret, and the optical components of the unit (8) are mounted on a plate (11) in an airtight and thermally sealed housing (12). The unit (8) is held at constant temperature by means of an inbuilt heat-emitting component, specifically a diode laser source and are so designed as to fit onto their plate (11) by special holders which allow for temperature compensation and so leave the optical adjustment of the modular unit (8) unchanged. The objective stage (14) sits horizontal with the housing (12) coupled up and is accessible from outside so it can be adjusted from outside e.g. by motor and is operated by joystick etc.
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公开(公告)号:DE10001954A1
公开(公告)日:2001-06-07
申请号:DE10001954
申请日:2000-01-18
Applicant: LEICA MICROSYSTEMS
Inventor: ENGELHARDT JOHANN , BRADL JOACHIM
IPC: G02B21/00
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公开(公告)号:DE19957418A1
公开(公告)日:2001-05-31
申请号:DE19957418
申请日:1999-11-29
Applicant: LEICA MICROSYSTEMS
Inventor: KNEBEL WERNER , BRADL JOACHIM
Abstract: The method involves preferably using confocal laser scanning microscopy with light intensity regulated according to the current focal position (2) in the object region (3) of the rastered, focused light beam. The regulation of the light intensity can be performed depending on the axial and/or lateral focal position using focal positions in an object region defined by the user. Independent claims are also included for the following: a raster microscope for using the method of optical scanning of an object.
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公开(公告)号:DE19906757A1
公开(公告)日:1999-12-02
申请号:DE19906757
申请日:1999-02-17
Applicant: LEICA MICROSYSTEMS
Inventor: ENGELHARDT JOHANN , ULRICH HEINRICH , BRADL JOACHIM
Abstract: The arrangement has at least one spectrally selective element (4) for coupling the stimulation light from at least one light source (2) into the microscope and for stopping the stimulation light reflected and scattered at the object (10) and the stimulation wavelengths from the light passing from the object over the detection beam path (12). Stimulation light of different wavelengths can be stopped by the spectrally selective element.
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公开(公告)号:LU92807B1
公开(公告)日:2017-03-01
申请号:LU92807
申请日:2015-08-27
Applicant: LEICA MICROSYSTEMS
Inventor: BRADL JOACHIM , FAHRBACH FLORIAN , KNEBEL WERNER
Abstract: Die Erfindung betrifft eine Beleuchtungsanordnung für ein Lichtblatt-Mikroskop, bei dem eine Probe mit einem Beleuchtungslichtbündel (8) beleuchtet wird, das im Bereich der Probe als Lichtblatt (11) ausgebildet ist, mit einem Beleuchtungsobjektiv (4), einer Tubuslinse (3) und einer astigmatischen Optik (7). Die Beleuchtungsanordnung zeichnet sich dadurch aus, dass die astigmatische Optik (7) zwischen der Tubuslinse (3) und dem Beleuchtungsobjektiv (4) angeordnet ist und/oder keine weiteren fokussierenden oder defokussierenden Elemente zwischen der astigmatischen Optik (7) und dem Beleuchtungsobjektiv (4) angeordnet sind. Alternativ kann auch vorgesehen sein, dass die astigmatische Optik Teil des Beleuchtungsobjektivs (4) ist. Darüber hinaus betrifft die Erfindung ein Verfahren zum Beleuchten einer Probe mittels einer solchen Beleuchtungsanordnung, sowie ein Mikroskop, das eine solche Beleuchtungsanordnung beinhaltet. (Fig. 1)
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公开(公告)号:DE10001954B4
公开(公告)日:2004-07-22
申请号:DE10001954
申请日:2000-01-18
Applicant: LEICA MICROSYSTEMS
Inventor: ENGELHARDT JOHANN , BRADL JOACHIM
IPC: G02B21/00
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公开(公告)号:DE19906757B4
公开(公告)日:2004-07-15
申请号:DE19906757
申请日:1999-02-17
Applicant: LEICA MICROSYSTEMS
Inventor: ENGELHARDT JOHANN , ULRICH HEINRICH , BRADL JOACHIM
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