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    Method and apparatus for measuring analyte using large scale fet array
    2.
    发明专利
    Method and apparatus for measuring analyte using large scale fet array 有权
    使用大规模场效应管阵列测量分析仪的方法和装置

    公开(公告)号：JP2013081463A

    公开(公告)日：2013-05-09

    申请号：JP2012246413

    申请日：2012-11-08

    Applicant: Life Technologies Corp ,  ライフ テクノロジーズ コーポレーション

    Inventor： ROTHBERG JONATHAN M ,  HINZ WOLFGANG ,  JOHNSON KIM L ,  BUSTILLO JAMES

    IPC: C12Q1/68 ,  C12M1/00 ,  C12N15/09 ,  G01N27/414 ,  G01N33/53

    CPC classification number: G01N27/4148 ,  C12Q1/6818 ,  C12Q1/6874 ,  G01N27/4145 ,  G01N33/54373 ,  G01N33/5438 ,  H01L27/088 ,  H01L29/42324 ,  H01L29/78 ,  H01L2924/01006 ,  H01L2924/01013 ,  H01L2924/01015 ,  H01L2924/01073 ,  H01L2924/14 ,  H01L2924/1433 ,  Y10T436/22 ,  C12Q2565/607 ,  C12Q2565/301 ,  C12Q2533/101

    Abstract: PROBLEM TO BE SOLVED: To detect a presence and/or concentration changes of various analyte types in a wide variety of chemical and/or biological processes, and to facilitate DNA sequencing techniques based on monitoring changes in hydrogen ion concentration (pH).SOLUTION: ChemFET arrays are fabricated using conventional CMOS processing techniques based on improved FET pixel and array designs that increase measurement sensitivity and accuracy, and at the same time facilitate significantly small pixel sizes and dense arrays. A plurality of template nucleic acids are disposed into a plurality of reaction chambers (wherein the plurality of reaction chambers is incontact with a chemFET array), a new nucleic acid strand is synthesized by incorporating one or more known nucleotide triphosphates sequentially at the 3' end of the sequencing primer, and the incorporation of the one or more known nucleotide triphosphates is detected by a change in current at the at least one chemFET in the array.

    Abstract translation: 要解决的问题：为了检测各种化学和/或生物过程中各种分析物类型的存在和/或浓度变化，并且基于监测氢离子浓度（pH）的变化来促进DNA测序技术， 。 解决方案：ChemFET阵列使用基于改进的FET像素和阵列设计的传统CMOS处理技术制造，增加了测量灵敏度和精度，并同时促进了显着小的像素尺寸和密集阵列。 将多个模板核酸设置在多个反应室中（其中多个反应室与chemFET阵列接触），通过在3'末端顺序掺入一种或多种已知的核苷酸三磷酸合成新的核酸链 的测序引物，并且通过阵列中的至少一个chemFET上的电流变化来检测一种或多种已知的核苷酸三磷酸的引入。 版权所有（C）2013，JPO＆INPIT

    ISFET SENSOR ARRAY COMPRISING TITANIUM NITRIDE AS A SENSING LAYER LOCATED ON THE BOTTOM OF A MICROWELL STRUCTURE
    3.
    发明申请
    ISFET SENSOR ARRAY COMPRISING TITANIUM NITRIDE AS A SENSING LAYER LOCATED ON THE BOTTOM OF A MICROWELL STRUCTURE 审中-公开
    ISFET传感器阵列包含硝酸铁作为位于MICROWELL结构底部的传感层

    公开(公告)号：WO2013109886A8

    公开(公告)日：2014-02-27

    申请号：PCT/US2013022140

    申请日：2013-01-18

    Applicant: LIFE TECHNOLOGIES CORP ,  BUSTILLO JAMES ,  REARICK TODD ,  HINZ WOLFGANG ,  FIFE KEITH

    Inventor： BUSTILLO JAMES ,  REARICK TODD ,  HINZ WOLFGANG ,  FIFE KEITH

    IPC: G01N27/414

    CPC classification number: B01L3/5027 ,  B01L3/502707 ,  B01L2200/0668 ,  B01L2300/0636 ,  B01L2300/0645 ,  B01L2300/0819 ,  B01L2300/0877 ,  B01L2300/0893 ,  G01N27/4145

    Abstract: A method of fabricating a microwell in an array structure is disclosed herein. The array structure includes a plurality of field effect transistors (FETs), where each FET has a gate structure. The method includes disposing a titanium nitride (TiN) layer on at least one conductive layer coupled to the gate structure of at least one FET. A insulation layer is disposed on the array structure, where the insulation layer lies above the TiN layer. Further, an opening above the gate structure of the at least one FET is etched to remove the insulation layer above the gate structure and to expose the TiN layer. A microwell with at least one sidewall formed from the insulation layer and with a bottom surface formed from the TiN layer is a result of the etching process. The gate structure is specified as a floating gate structure and the FET is an ISFET.

    Abstract translation: 本文公开了一种制造阵列结构中的微孔的方法。 阵列结构包括多个场效应晶体管（FET），其中每个FET具有栅极结构。 该方法包括在耦合到至少一个FET的栅极结构的至少一个导电层上设置氮化钛（TiN）层。 绝缘层设置在阵列结构上，其中绝缘层位于TiN层上方。 此外，蚀刻至少一个FET的栅极结构之上的开口以去除栅极结构上方的绝缘层并暴露TiN层。 具有由绝缘层形成的至少一个侧壁和由TiN层形成的底表面的微孔是蚀刻工艺的结果。 栅极结构被指定为浮置栅极结构，FET是ISFET。

    MODIFIED PROTEINS AND METHODS OF MAKING AND USING SAME
    4.
    发明申请
    MODIFIED PROTEINS AND METHODS OF MAKING AND USING SAME 审中-公开
    修饰的蛋白质及其制备和使用方法

    公开(公告)号：WO2011106634A3

    公开(公告)日：2012-05-03

    申请号：PCT/US2011026228

    申请日：2011-02-25

    Applicant: LIFE TECHNOLOGIES CORP ,  DAVIDSON JOHN F ,  HINZ WOLFGANG ,  ROTHBERG JONATHAN ,  WHITAKER RICHARD

    Inventor： DAVIDSON JOHN F ,  HINZ WOLFGANG ,  ROTHBERG JONATHAN ,  WHITAKER RICHARD

    IPC: C12Q1/68 ,  C12N9/12 ,  C12Q1/48

    CPC classification number: C12N9/1252

    Abstract: Methods, compositions, systems, apparatuses and kits comprising modified proteins, particularly modified nucleic acid-binding proteins with altered buffering properties are provided. For example, in some embodiments, methods of forming modified proteins including one or more amino acid modifications to achieve desired pKa values are described. Furthermore, the invention provides methods for using such modified proteins in ion-producing reactions, such as ion-based nucleic acid sequencing reactions.

    Abstract translation: 提供了包含修饰的蛋白质，特别是具有改变的缓冲性质的修饰的核酸结合蛋白的方法，组合物，系统，装置和试剂盒。 例如，在一些实施方案中，描述了形成包含一个或多个氨基酸修饰的修饰蛋白以实现期望的pKa值的方法。 此外，本发明提供了在离子生成反应中使用这些修饰蛋白的方法，如基于离子的核酸测序反应。

    CHEMICAL SENSOR WITH CONDUCTIVE CUP-SHAPED SENSOR SURFACE
    7.
    发明申请
    CHEMICAL SENSOR WITH CONDUCTIVE CUP-SHAPED SENSOR SURFACE 审中-公开
    具有导电杯形传感器表面的化学传感器

    公开(公告)号：WO2013109877A3

    公开(公告)日：2013-10-03

    申请号：PCT/US2013022129

    申请日：2013-01-18

    Applicant: LIFE TECHNOLOGIES CORP ,  LI SHIFENG ,  BUSTILLO JAMES ,  HINZ WOLFGANG

    Inventor： LI SHIFENG ,  BUSTILLO JAMES ,  HINZ WOLFGANG

    IPC: G01N27/414

    CPC classification number: G01N27/4145 ,  C12Q1/6869 ,  G01N27/414

    Abstract: A chemical detection device and a method of its manufacture is described herein. The device includes a chemically-sensitive field effect transistor 214 including a floating gate conductor 218 having an upper surface 220. A dielectric material 210 defines a cavity 201 extending to the upper surface of the floating gate conductor. A conductive layer 250 is on a sidewall 260 of the cavity and electrically communicating with the floating gate conductor 220. An inner surface 251 of the conductive layer 250 defines a well 201 for the sensor.

    Abstract translation: 本文描述了化学检测装置及其制造方法。 该器件包括化学敏感的场效应晶体管214，其包括具有上表面220的浮栅导体218.介电材料210限定延伸到浮栅导体的上表面的空腔201。 导电层250在空腔的侧壁260上并与浮动栅极导体220电连通。导电层250的内表面251限定用于传感器的阱201。

    MODIFIED PROTEINS AND METHODS OF MAKING AND USING SAME
    10.
    发明申请
    MODIFIED PROTEINS AND METHODS OF MAKING AND USING SAME 审中-公开
    改良蛋白质及其制备方法和使用方法

    公开(公告)号：WO2011106629A3

    公开(公告)日：2012-01-19

    申请号：PCT/US2011026219

    申请日：2011-02-25

    Applicant: LIFE TECHNOLOGIES CORP ,  DAVIDSON JOHN F ,  HINZ WOLFGANG ,  ROTHBERG JONATHAN M ,  WHITAKER RICHARD

    Inventor： DAVIDSON JOHN F ,  HINZ WOLFGANG ,  ROTHBERG JONATHAN M ,  WHITAKER RICHARD

    IPC: C12Q1/68 ,  C12N9/12 ,  C12Q1/48

    CPC classification number: C12N9/1252

    Abstract: Methods, compositions, systems, apparatuses and kits comprising modified proteins, particularly modified nucleic acid-binding proteins with altered buffering properties are provided. For example, in some embodiments, methods of forming modified proteins including one or more amino acid modifications to achieve desired pKa values are described. Furthermore, the invention provides methods for using such modified proteins in ion-producing reactions, such as ion-based nucleic acid sequencing reactions.

    Abstract translation: 提供了包含修饰的蛋白质，特别是具有改变的缓冲性质的经修饰的核酸结合蛋白质的方法，组合物，系统，装置和试剂盒。 例如，在一些实施方案中，描述了形成包含一个或多个氨基酸修饰以获得所需pKa值的修饰蛋白质的方法。 此外，本发明提供了在离子产生反应中使用这些修饰蛋白质的方法，例如基于离子的核酸测序反应。

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