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    METHOD AND APPARATUS FOR ADDRESSABLE FLOW CELLS IN SINGLE MOLECULE SEQUENCING
    1.
    发明申请
    METHOD AND APPARATUS FOR ADDRESSABLE FLOW CELLS IN SINGLE MOLECULE SEQUENCING 审中-公开
    单分子序列中可寻址流动细胞的方法和装置

    公开(公告)号：WO2011116120A2

    公开(公告)日：2011-09-22

    申请号：PCT/US2011028704

    申请日：2011-03-16

    Applicant: LIFE TECHNOLOGIES CORP ,  LAFFERTY W MICHAEL ,  BEECHEM JOSEPH ,  SUN HONGYE ,  METZKER MICHAEL

    Inventor： LAFFERTY W MICHAEL ,  BEECHEM JOSEPH ,  SUN HONGYE ,  METZKER MICHAEL

    IPC: C12Q1/68 ,  C12N15/11 ,  G01N33/50

    CPC classification number: C12Q1/6874 ,  C12Q2523/319 ,  C12Q2525/186 ,  C12Q2565/513

    Abstract: A method of sequencing a plurality of template nucleotide sequences includes immobilizing the plurality of template nucleotide sequences on a substrate. A first subset of the plurality of template nucleotide sequences is immobilized in a first field of view and a second subset of the plurality of template nucleotide sequences is immobilized in a second field of view. The first and second subsets are hybridized to a caged primer. The caged primer includes a caging group. The method further includes lysing the caging group from the caged primer in the first field of view and observing the first field of view to detect sequencing of the first subset of the plurality of template nucleotide sequences.

    Abstract translation: 对多个模板核苷酸序列进行测序的方法包括将多个模板核苷酸序列固定在底物上。 多个模板核苷酸序列的第一子集被固定在第一视场中，并且多个模板核苷酸序列的第二子集被固定在第二视野中。 第一和第二子集与笼式引物杂交。 笼状引物包括笼养组。 该方法还包括在第一视场中从笼式引物裂解笼基组，并观察第一视野以检测多个模板核苷酸序列的第一子集的测序。

    DNA SEQUENCING METHODS AND DETECTORS AND SYSTEMS FOR CARRYING OUT THE SAME
    2.
    发明申请
    DNA SEQUENCING METHODS AND DETECTORS AND SYSTEMS FOR CARRYING OUT THE SAME 审中-公开
    DNA序列方法和检测器及其执行系统

    公开(公告)号：WO2011082419A3

    公开(公告)日：2012-02-09

    申请号：PCT/US2011020117

    申请日：2011-01-04

    Applicant: LIFE TECHNOLOGIES CORP ,  SUN HONGYE ,  NORDMAN ERIC S ,  OLDHAM MARK F ,  O'NEILL JOHN R ,  CONNELL CHARLES ,  ULMANELLA UMBERTO ,  LAU ALDRICH N K ,  KOTSEROGLOU THEOFILOS ,  LIVAK KENNETH J

    Inventor： SUN HONGYE ,  NORDMAN ERIC S ,  OLDHAM MARK F ,  O'NEILL JOHN R ,  CONNELL CHARLES ,  ULMANELLA UMBERTO ,  LAU ALDRICH N K ,  KOTSEROGLOU THEOFILOS ,  LIVAK KENNETH J

    IPC: C12Q1/68 ,  G01N27/414 ,  G01N33/50

    CPC classification number: C12Q1/6869 ,  B01L3/502761 ,  G01N27/3278 ,  G01N27/414 ,  G01N27/4145 ,  G01N27/4146 ,  G01N33/5438 ,  Y10S977/924 ,  Y10T436/143333 ,  C12Q2565/631

    Abstract: In some embodiments, an analyte detection system is provided that includes a nanochannel, an electrode arrangement, and a plurality of nanoFET devices disposed in the nanochannel. A plurality of nucleic acid base detection components can be used that include a plurality of nanopores, a plurality of nanochannels, a plurality of hybridization probes, combinations thereof, and the like. According to other embodiments of the present teachings, different coded molecules are hybridized to a target DNA molecule and used to detect the presence of various sequences along the target molecule. A kit including mixtures of coded molecules is also provided. In some embodiments, devices including nanochannels, nanopores, and the like, are used for manipulating movement of DNA molecules, for example, in preparation for a DNA sequencing detection. Nanopore structures and methods of making the same are also provided as are methods of nucleic acid sequencing using the nanopore structures. Surface-modified nanopores are provided as are methods of making them. In some embodiments, surfaced-modified nanopores for slowing the translocation of single stranded DNA (ssDNA) through the nanopore are provided, as are nanopores configured to detect each of a plurality of different bases on an ssDNA strand.

    Abstract translation: 在一些实施例中，提供了分析物检测系统，其包括纳米通道，电极装置和设置在纳米通道中的多个纳米器件。 可以使用多个核酸碱基检测组分，其包括多个纳米孔，多个纳米通道，多个杂交探针及其组合等。 根据本教导的其他实施方案，将不同的编码分子与靶DNA分子杂交并用于检测沿着靶分子的各种序列的存在。 还提供了包含编码分子混合物的试剂盒。 在一些实施方案中，包括纳米通道，纳米孔等的装置用于操纵DNA分子的移动，例如用于DNA测序检测的准备。 还提供了制备其的纳米孔结构及其制备方法，使用纳米孔结构的核酸测序方法也是如此。 提供表面改性的纳米孔也是制备它们的方法。 在一些实施方案中，提供用于减缓单链DNA（ssDNA）穿过纳米孔的易位的表面改性的纳米孔，以及配置成检测ssDNA链上的多个不同碱基中的每一个的纳米孔。

    BIOMOLECULAR SENSING WITH METAL-NANOSTRUCTURES
    3.
    发明申请
    BIOMOLECULAR SENSING WITH METAL-NANOSTRUCTURES 审中-公开
    生物分子与金属纳米结构的传感

    公开(公告)号：WO2010115147A3

    公开(公告)日：2011-01-13

    申请号：PCT/US2010029841

    申请日：2010-04-02

    Applicant: LIFE TECHNOLOGIES CORP ,  SUN HONGYE ,  OLDHAM MARK

    Inventor： SUN HONGYE ,  OLDHAM MARK

    IPC: G01N33/48 ,  C12Q1/68

    CPC classification number: C12Q1/6825 ,  C12Q1/6832 ,  C12Q2563/155 ,  C12Q2563/107 ,  C12Q2521/101 ,  C12Q2565/607

    Abstract: Systems and methods for providing biomolecule interaction sites are disclosed. In certain embodiments, coupling of two or more different metal-nanoparticles can provide a significant increase in flexibility in controlling parameters associated with excitation of fluorophores attached to the biomolecules. Types and number of metal-nanoparticles and spatial arrangement of such nanoparticles are some of the parameters that can be adjusted to control fluorescence parameters such as excitation wavelengths and intensity of fluorescence. In certain embodiments, enhanced fluorescence effect can be confined to a sufficiently small region so as to allow sensing of one biomolecule at a time. Combined with enhanced fluorescence intensity, such small enhancement region can be used to perform processes such as real-time DNA sequencing.

    Abstract translation: 公开了提供生物分子相互作用位点的系统和方法。 在某些实施方案中，两种或更多种不同的金属纳米颗粒的偶联可以提供在控制与生物分子附着的荧光团的激发相关联的参数中的灵活性的显着增加。 金属纳米颗粒的种类和数量以及这些纳米颗粒的空间布置是可以调整以控制荧光参数如激发波长和荧光强度的一些参数。 在某些实施方案中，增强的荧光效应可以被限制在足够小的区域，以允许一次检测一个生物分子。 结合增强的荧光强度，这种小增强区域可用于进行诸如实时DNA测序的过程。

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