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    DNA SEQUENCING METHODS AND DETECTORS AND SYSTEMS FOR CARRYING OUT THE SAME
    1.
    发明申请
    DNA SEQUENCING METHODS AND DETECTORS AND SYSTEMS FOR CARRYING OUT THE SAME 审中-公开
    DNA序列方法和检测器及其执行系统

    公开(公告)号：WO2011082419A3

    公开(公告)日：2012-02-09

    申请号：PCT/US2011020117

    申请日：2011-01-04

    Applicant: LIFE TECHNOLOGIES CORP ,  SUN HONGYE ,  NORDMAN ERIC S ,  OLDHAM MARK F ,  O'NEILL JOHN R ,  CONNELL CHARLES ,  ULMANELLA UMBERTO ,  LAU ALDRICH N K ,  KOTSEROGLOU THEOFILOS ,  LIVAK KENNETH J

    Inventor： SUN HONGYE ,  NORDMAN ERIC S ,  OLDHAM MARK F ,  O'NEILL JOHN R ,  CONNELL CHARLES ,  ULMANELLA UMBERTO ,  LAU ALDRICH N K ,  KOTSEROGLOU THEOFILOS ,  LIVAK KENNETH J

    IPC: C12Q1/68 ,  G01N27/414 ,  G01N33/50

    CPC classification number: C12Q1/6869 ,  B01L3/502761 ,  G01N27/3278 ,  G01N27/414 ,  G01N27/4145 ,  G01N27/4146 ,  G01N33/5438 ,  Y10S977/924 ,  Y10T436/143333 ,  C12Q2565/631

    Abstract: In some embodiments, an analyte detection system is provided that includes a nanochannel, an electrode arrangement, and a plurality of nanoFET devices disposed in the nanochannel. A plurality of nucleic acid base detection components can be used that include a plurality of nanopores, a plurality of nanochannels, a plurality of hybridization probes, combinations thereof, and the like. According to other embodiments of the present teachings, different coded molecules are hybridized to a target DNA molecule and used to detect the presence of various sequences along the target molecule. A kit including mixtures of coded molecules is also provided. In some embodiments, devices including nanochannels, nanopores, and the like, are used for manipulating movement of DNA molecules, for example, in preparation for a DNA sequencing detection. Nanopore structures and methods of making the same are also provided as are methods of nucleic acid sequencing using the nanopore structures. Surface-modified nanopores are provided as are methods of making them. In some embodiments, surfaced-modified nanopores for slowing the translocation of single stranded DNA (ssDNA) through the nanopore are provided, as are nanopores configured to detect each of a plurality of different bases on an ssDNA strand.

    Abstract translation: 在一些实施例中，提供了分析物检测系统，其包括纳米通道，电极装置和设置在纳米通道中的多个纳米器件。 可以使用多个核酸碱基检测组分，其包括多个纳米孔，多个纳米通道，多个杂交探针及其组合等。 根据本教导的其他实施方案，将不同的编码分子与靶DNA分子杂交并用于检测沿着靶分子的各种序列的存在。 还提供了包含编码分子混合物的试剂盒。 在一些实施方案中，包括纳米通道，纳米孔等的装置用于操纵DNA分子的移动，例如用于DNA测序检测的准备。 还提供了制备其的纳米孔结构及其制备方法，使用纳米孔结构的核酸测序方法也是如此。 提供表面改性的纳米孔也是制备它们的方法。 在一些实施方案中，提供用于减缓单链DNA（ssDNA）穿过纳米孔的易位的表面改性的纳米孔，以及配置成检测ssDNA链上的多个不同碱基中的每一个的纳米孔。

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