公开(公告)号：WO2012112609A2

公开(公告)日：2012-08-23

申请号：PCT/US2012/025123

申请日：2012-02-14

Applicant: LIFE TECHNOLOGIES CORPORATION ,  LAKSHMIPATHY, Uma ,  SINGH, Upinder ,  GRECIAN, Scott ,  QUINTANILLA, Rene ,  GEE, Kyle ,  RAO, Mahendra

Inventor： LAKSHMIPATHY, Uma ,  SINGH, Upinder ,  GRECIAN, Scott ,  QUINTANILLA, Rene ,  GEE, Kyle ,  RAO, Mahendra

IPC: G01N33/58 ,  G01N33/50

CPC classification number: G01N33/5005 ,  C09B11/24 ,  C09B19/00 ,  C09B21/00 ,  C09B57/00 ,  C09B57/02 ,  C12Q1/42 ,  C12Y301/03001 ,  G01N1/30 ,  G01N33/5073 ,  G01N33/581 ,  G01N33/582 ,  G01N33/586 ,  G01N2001/302 ,  G01N2333/916

Abstract: The invention relates to novel substrates and methods for staining live stem cells. The stain may be used to identify induced pluripotent stem cell colonies during the process of somatic cell reprogramming.

Abstract translation: 本发明涉及用于染色活的干细胞的新型底物和方法。 该染色剂可用于在体细胞重编程过程中鉴定诱导的多能干细胞集落。

公开(公告)号：WO2012016048A1

公开(公告)日：2012-02-02

申请号：PCT/US2011/045731

申请日：2011-07-28

Applicant: LIFE TECHNOLOGIES CORPORATION ,  AGNEW, Brian ,  GRAHAM, David ,  SINGH, Upinder ,  GRECIAN, Scott

Inventor： AGNEW, Brian ,  GRAHAM, David ,  SINGH, Upinder ,  GRECIAN, Scott

IPC: A61K31/00 ,  A61K31/19 ,  A61K31/20 ,  A61P31/00 ,  A61P31/18

CPC classification number: A61K31/655 ,  A61K31/7024 ,  A61K31/7088 ,  A61K38/212 ,  A61K38/215 ,  A61K45/06 ,  A61K2300/00

Abstract: Methods of using azide-modified biomolecules, such as fatty acids, carbohydrates and lipids, to treat a plant, an insect or an animal infected with a virus or to inhibit infectivity of a virus, such as the human immunodeficiency virus, are provided. Also provided are methods of labeling a virus, such as human immunodeficiency virus, with an azide-modified biomolecule, such as a fatty acid, a carbohydrate, or an isoprenoid lipid. Also, provided are methods of tracking a virus in vivo , with an azide-modified biomolecule, such as a fatty acid, a carbohydrate, or an isoprenoid lipid. The azide-modified biomolecules may be combined with a pharmaceutically acceptable excipient to produce a pharmaceutical composition, optionally containing another anti-viral agent and/or a delivery agent, such as a liposome.

Abstract translation: 提供了使用叠氮化物修饰的生物分子如脂肪酸，碳水化合物和脂质来治疗植物，昆虫或感染病毒的动物或抑制病毒如人类免疫缺陷病毒的感染性的方法。 还提供了用叠氮化物修饰的生物分子如脂肪酸，碳水化合物或类异戊二烯脂质来标记病毒（例如人类免疫缺陷病毒）的方法。 此外，提供了利用叠氮化物修饰的生物分子如脂肪酸，碳水化合物或类异戊二烯脂质体内追踪病毒的方法。 叠氮化物修饰的生物分子可以与药学上可接受的赋形剂组合以产生药物组合物，任选地含有另一种抗病毒剂和/或递送剂，例如脂质体。

公开(公告)号：WO2013180813A1

公开(公告)日：2013-12-05

申请号：PCT/US2013/031637

申请日：2013-03-14

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： GEE, Kyle ,  SINGH, Upinder ,  RUKAVISHNIKOV, Aleksey ,  BEACHAM, Daniel ,  HUANG, Shih-Jung ,  JANES, Michael ,  ZHOU, Wenjun

IPC: C09B11/24 ,  C07K1/13 ,  G01N33/52

CPC classification number: C09B11/24 ,  A61K49/0041 ,  C07D311/90 ,  G01N33/5005 ,  G01N33/582 ,  G01N33/84

Abstract: Disclosed herein are compounds, compositions, methods and kits for detecting pH in samples using pH-sensitive fluorescent dyes. The compounds disclosed herein are novel xanthene-derivative dyes comprising an aniline moiety with one or more electron donating groups, which dyes are for detecting pH in samples either in vitro or in vivo. Also described herein are processes for preparing said dyes for use in the disclosed compositions, methods and kits.

Abstract translation: 本文公开了使用pH敏感荧光染料检测样品中的pH的化合物，组合物，方法和试剂盒。 本文公开的化合物是包含具有一个或多个给电子基团的苯胺部分的新型呫吨衍生物染料，该染料用于在体外或体内检测样品中的pH。 本文还描述了用于制备用于所公开的组合物，方法和试剂盒的所述染料的方法。

公开(公告)号：WO2012016044A1

公开(公告)日：2012-02-02

申请号：PCT/US2011/045726

申请日：2011-07-28

Applicant: LIFE TECHNOLOGIES CORPORATION ,  AGNEW, Brian ,  SINGH, Upinder ,  GRECIAN, Scott

Inventor： AGNEW, Brian ,  SINGH, Upinder ,  GRECIAN, Scott

IPC: A61K31/00 ,  A61K31/19 ,  A61K31/20 ,  C12N7/04

CPC classification number: A61K31/655 ,  A61K31/7008 ,  C12N7/00 ,  C12N2710/14151 ,  C12N2710/14163 ,  C12N2740/16051 ,  C12N2740/16063

Abstract: Methods of using azide-modified biomolecules, such as fatty acids, carbohydrates and lipids, to treat a plant, an insect or an animal infected with a virus or to inhibit infectivity of a virus, such as the human immunodeficiency virus, are provided. Also provided are methods of labeling a virus, such as human immunodeficiency virus, with an azide-modified biomolecule, such as a fatty acid, a carbohydrate, or an isoprenoid lipid. Also, provided are methods of tracking a virus in vivo, with an azide-modified biomolecule, such as a fatty acid, a carbohydrate, or an isoprenoid lipid. The azide-modified biomolecules may be combined with a pharmaceutically acceptable excipient to produce a pharmaceutical composition, optionally containing another anti-viral agent and/or a delivery agent, such as a liposome.

Abstract translation: 提供了使用叠氮化物修饰的生物分子如脂肪酸，碳水化合物和脂质来治疗植物，昆虫或感染病毒的动物或抑制病毒如人类免疫缺陷病毒的感染性的方法。 还提供了用叠氮化物修饰的生物分子如脂肪酸，碳水化合物或类异戊二烯脂质来标记病毒（例如人类免疫缺陷病毒）的方法。 此外，提供了利用叠氮化物修饰的生物分子如脂肪酸，碳水化合物或类异戊二烯脂质体内追踪病毒的方法。 叠氮化物修饰的生物分子可以与药学上可接受的赋形剂组合以产生药物组合物，任选地含有另一种抗病毒剂和/或递送剂，例如脂质体。

公开(公告)号：WO2012121973A1

公开(公告)日：2012-09-13

申请号：PCT/US2012/027285

申请日：2012-03-01

Applicant: LIFE TECHNOLOGIES CORPORATION ,  GEE, Kyle ,  SINGH, Upinder ,  GRECIAN, Scott

Inventor： GEE, Kyle ,  SINGH, Upinder ,  GRECIAN, Scott

IPC: C07D213/78

CPC classification number: G01N33/582 ,  C07D213/53 ,  C07D213/68 ,  C07D213/74 ,  C07D213/78 ,  C07D213/79 ,  C07D213/81 ,  C07D215/12 ,  C07D235/14 ,  C07D401/04 ,  C07D401/14 ,  C07K14/575 ,  C09B11/24 ,  C09B23/06 ,  C09B23/083 ,  G01N33/533

Abstract: Low-copper click chemistry, 1.3-dipolar cycloadditions, and Staudinger ligations for modifying biomolecules is provided. Compositions, methods, and kits relating to low-copper click chemistry, 1.3-dipolar cycloadditions, and Staudinger ligations are also provided.

Abstract translation: 提供了低铜点击化学，1.3偶极环加成和用于修饰生物分子的Staudinger连接。 还提供了与低铜点击化学，1.3偶极环加成和Staudinger连接有关的组合物，方法和试剂盒。

公开(公告)号：WO2013180811A1

公开(公告)日：2013-12-05

申请号：PCT/US2013/031535

申请日：2013-03-14

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： GEE, Kyle ,  SINGH, Upinder ,  RUKAVISHNIKOV, Aleksey ,  BEACHAM, Daniel ,  HUANG, Shih-Jung ,  JANES, Michael ,  ZHOU, Wenjun ,  BERLIER, Judith

IPC: C09B11/24 ,  C07K1/13 ,  G01N33/52

CPC classification number: C09B11/24 ,  A61K49/0041 ,  C07D311/90 ,  G01N33/5005 ,  G01N33/582 ,  G01N33/84

Abstract: Disclosed herein are compounds, compositions, methods and kits for detecting pH in samples using pH-sensitive fluorescent dyes. The compounds disclosed herein are novel xanthene-derivative dyes comprising an aniline moiety with one or more electron donating groups, which dyes are for detecting pH in samples either in vitro or in vivo. Also described herein are processes for preparing said dyes for use in the disclosed compositions, methods and kits.

Abstract translation: 本文公开了使用pH敏感荧光染料检测样品中的pH的化合物，组合物，方法和试剂盒。 本文公开的化合物是包含具有一个或多个给电子基团的苯胺部分的新型呫吨衍生物染料，该染料用于在体外或体内检测样品中的pH。 本文还描述了用于制备用于所公开的组合物，方法和试剂盒的所述染料的方法。

公开(公告)号：WO2011079188A2

公开(公告)日：2011-06-30

申请号：PCT/US2010/061768

申请日：2010-12-22

Applicant: LIFE TECHNOLOGIES CORPORATION ,  AGNEW, Brian ,  PICKENS, Chad ,  SINGH, Upinder

Inventor： AGNEW, Brian ,  PICKENS, Chad ,  SINGH, Upinder

IPC: C07C247/08 ,  C07C233/09 ,  G01N33/68

CPC classification number: C07C247/04 ,  C07C233/09 ,  G01N33/5008 ,  G01N33/5308 ,  G01N33/68 ,  G01N33/6842 ,  G01N2440/00 ,  G01N2440/12

Abstract: Clickable polyunsaturated fatty acid analogs, methods of using these analogs and kits comprising these analogs.

Abstract translation: 可点击的多不饱和脂肪酸类似物，使用这些类似物的方法和包含这些类似物的试剂盒。

公开(公告)号：EP2681196A1

公开(公告)日：2014-01-08

申请号：EP12710600.3

申请日：2012-03-01

Applicant: Life Technologies Corporation

Inventor： GEE, Kyle ,  SINGH, Upinder ,  GRECIAN, Scott

IPC: C07D213/78

CPC classification number: G01N33/582 ,  C07D213/53 ,  C07D213/68 ,  C07D213/74 ,  C07D213/78 ,  C07D213/79 ,  C07D213/81 ,  C07D215/12 ,  C07D235/14 ,  C07D401/04 ,  C07D401/14 ,  C07K14/575 ,  C09B11/24 ,  C09B23/06 ,  C09B23/083 ,  G01N33/533

Abstract: Low-copper click chemistry, 1.3-dipolar cycloadditions, and Staudinger ligations for modifying biomolecules is provided. Compositions, methods, and kits relating to low-copper click chemistry, 1.3-dipolar cycloadditions, and Staudinger ligations are also provided.

公开(公告)号：EP2875075A1

公开(公告)日：2015-05-27

申请号：EP13713030.8

申请日：2013-03-14

Applicant: Life Technologies Corporation

Inventor： GEE, Kyle ,  SINGH, Upinder ,  RUKAVISHNIKOV, Aleksey ,  BEACHAM, Daniel ,  HUANG, Shih-Jung ,  JANES, Michael ,  ZHOU, Wenjun

IPC: C09B11/24 ,  C07K1/13 ,  G01N33/52

CPC classification number: C09B11/24 ,  A61K49/0041 ,  C07D311/90 ,  G01N33/5005 ,  G01N33/582 ,  G01N33/84

Abstract: Disclosed herein are compounds, compositions, methods and kits for detecting pH in samples using pH-sensitive fluorescent dyes. The compounds disclosed herein are novel xanthene-derivative dyes comprising an aniline moiety with one or more electron donating groups, which dyes are for detecting pH in samples either in vitro or in vivo. Also described herein are processes for preparing said dyes for use in the disclosed compositions, methods and kits.

Abstract translation: 本文公开了使用pH敏感荧光染料检测样品中的pH的化合物，组合物，方法和试剂盒。 本文公开的化合物是包含具有一个或多个给电子基团的苯胺部分的新型呫吨衍生物染料，该染料用于在体外或体内检测样品中的pH。 本文还描述了用于制备用于所公开的组合物，方法和试剂盒的所述染料的方法。

公开(公告)号：EP2855596B1

公开(公告)日：2019-02-27

申请号：EP13714770.8

申请日：2013-03-14

Applicant: Life Technologies Corporation

Inventor： GEE, Kyle ,  SINGH, Upinder ,  RUKAVISHNIKOV, Aleksey ,  BEACHAM, Daniel ,  HUANG, Shih-Jung ,  JANES, Michael ,  ZHOU, Wenjun ,  BERLIER, Judith

IPC: A61K49/00 ,  C07D311/90 ,  G01N33/58 ,  G01N33/84 ,  G01N33/50 ,  C09B11/24