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公开(公告)号：WO2013012463A1
公开(公告)日：2013-01-24
申请号：PCT/US2012/032571
申请日：2012-04-06
Applicant: LIFE TECHNOLOGIES CORPORATION , MARRAN, David , BEAUCHEMIN, Mark
Inventor： MARRAN, David , BEAUCHEMIN, Mark
IPC: G01N27/414
CPC classification number: G01N33/84 , C12Q1/6874
Abstract: A method of preparing a sequencing device includes determining a sensitivity of a pH of a solution to a first reagent, determining an amount of the first reagent to add to the solution to approach a target pH, adding the amount of the first reagent to the solution, and diluting a nucleotide solution with the solution.
Abstract translation: 制备测序装置的方法包括确定溶液的pH对第一试剂的灵敏度，确定添加到溶液中的第一试剂的量以接近目标pH，将第一试剂的量加到溶液中 ，并用溶液稀释核苷酸溶液。
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公开(公告)号：WO2021034709A1
公开(公告)日：2021-02-25
申请号：PCT/US2020/046502
申请日：2020-08-14
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor： HOSHIZAKI, Jon , LEUNG, Patrick , MARRAN, David , REED, Mark
Abstract: An apparatus includes a fluidic coupler including an opening. A first port is in fluid communication with the opening and is to interface with an inlet of a flow cell of a sensor device. A second port is to interface with an outlet of the flow cell of the sensor device. A third port is in fluidic communication with the second port. The apparatus further includes a mechanical assembly moveable between a first position and a second position. The fluidic coupler is secured to the flow cell of the sensor device in the first position. The fluidic coupler is disengaged from the flow cell of the sensor device in the second position.
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公开(公告)号：WO2020037286A1
公开(公告)日：2020-02-20
申请号：PCT/US2019/046945
申请日：2019-08-16
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor： XING, Zheng (Derek) , REED, Mark , MARRAN, David , ZERGIEBEL, Earl
IPC: B01L3/00 , G01N1/30 , G01N33/543 , G01N35/10 , G01N35/04
Abstract: In an example, a method for preparing a nucleotide solution includes flowing an aqueous solution from an initial solution storage of a sequencing instrument continuously through a container fluidically coupled to the sequencing instrument, the container comprising a nucleotide concentrate; and collecting the aqueous solution with nucleotide in a storage container.
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公开(公告)号：WO2022104272A1
公开(公告)日：2022-05-19
申请号：PCT/US2021/059538
申请日：2021-11-16
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor： PODHASKY, Joseph , REED, Mark , MARRAN, David , WONG, Chiu Tai Andrew , CHENG, Angie , CAO, Ru
IPC: B01L3/00 , C12Q1/6869
Abstract: A sequencing system includes an automated sequencing instrument adapted to determine variant calls for one or more extracted polynucleotide samples with a performance of at least 98.5% raw read accuracy and a total instrument run time in a range of 16 hours to 22 hours to determine variant calls when sequencing 6 cfTNA extracted polynucleotide samples using a targeted assay with one nucleic acid pool per sample.
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公开(公告)号：WO2010047804A8
公开(公告)日：2010-04-29
申请号：PCT/US2009/005745
申请日：2009-10-22
Applicant: LIFE TECHNOLOGIES CORPORATION , ROTHBERG, Jonathan, M. , SCHULTZ, Jonathan, C. , JOHNSON, Kim, L. , REARICK, Todd, M. , MILGREW, Mark, James , MARRAN, David , BUSTILLO, James, M.
Inventor： ROTHBERG, Jonathan, M. , SCHULTZ, Jonathan, C. , JOHNSON, Kim, L. , REARICK, Todd, M. , MILGREW, Mark, James , MARRAN, David , BUSTILLO, James, M.
IPC: G01N27/416 , C12M1/34
Abstract: The invention is directed to apparatus and chips comprising a large scale chemical field effect transistor arrays that include an array of sample-retaining regions capable of retaining a chemical or biological sample from a sample fluid for analysis. In one aspect such transistor arrays have a pitch of 10 μm or less and each sample-retaining region is positioned on at least one chemical field effect transistor which is configured to generate at least one output signal related to a characteristic of a chemical or biological sample in such sample-retaining region. In one embodiment, the characteristic of said chemical or biological sample is a concentration of a charged species and wherein each of said chemical field effect transistors is an ion-sensitive field effect transistor having a floating gate with a dielectric layer on a surface thereof, the dielectric layer contacting said sample fluid and being capable of accumulating charge in proportion to a concentration of the charged species in said sample fluid. In one embodiment such charged species is a hydrogen ion such that the sensors measure changes in pH of the sample fluid in or adjacent to the sample-retaining region thereof. Apparatus and chips of the invention may be adapted for large scale pH-based DNA sequencing and other bioscience and biomedical applications.
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Patent Agency Ranking
公开(公告)号：WO2021034711A1
公开(公告)日：2021-02-25
申请号：PCT/US2020/046510
申请日：2020-08-14
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor： REED, Mark , WONG, Chiu Tai Andrew , MARRAN, David
IPC: B01F13/00 , B01J19/00 , B01L3/00 , C12Q1/6806
Abstract: A sequencing system includes an automated sequencing instrument adapted to determine variant calls for one or more extracted polynucleotide samples with a performance of at least 98.5% raw read accuracy and a run time in a range of 5 hours to 14 hours to determine variant calls for 4 extracted polynucleotide samples using a targeted assay with one DNA pool per sample and an average amplicon size in a range of 100 to 120 bases.
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公开(公告)号：WO2011085258A2
公开(公告)日：2011-07-14
申请号：PCT/US2011/020590
申请日：2011-01-07
Applicant: LIFE TECHNOLOGIES CORPORATION , NOBILE, John , ROTH, George , MARRAN, David , MILESKI, William
Inventor： NOBILE, John , ROTH, George , MARRAN, David , MILESKI, William
IPC: G01N27/26 , G01N27/414 , G01N33/487 , C12Q1/68
CPC classification number: G01N27/4148 , Y10T29/49147
Abstract: A leak resistant electrical connector configured as a fluidic barrier between a fluidics device, which may comprise a chemFET sensor, and other electrical circuitry wherein the fluidics device further comprises one or more electrical contacts conductively coupled to one or more electrical contacts associated with the electrical circuitry through the connector.
Abstract translation: 一种防漏电连接器，其构造为流体装置之间的流体阻挡层，流体装置可包括chemFET传感器和其它电路，其中流体装置还包括一个或多个导电耦合到与电路相关联的一个或多个电触头的电触头 通过连接器。
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公开(公告)号：WO2010138182A3
公开(公告)日：2010-12-02
申请号：PCT/US2010/001543
申请日：2010-05-27
Applicant: LIFE TECHNOLOGIES CORPORATION , ROTHBERG, Jonathan M. , HINZ, Wolfgang , DAVIDSON, John F. , VAN OIJEN, Antoine M. , LEAMON, John H. , HUBER, Martin , BUSTILLO, James M. , MILGREW, Mark, James , SCHULTZ, Jonathan , MARRAN, David , REARICK, Todd , JOHNSON, Kim L.
Inventor： ROTHBERG, Jonathan M. , HINZ, Wolfgang , DAVIDSON, John F. , VAN OIJEN, Antoine M. , LEAMON, John H. , HUBER, Martin , BUSTILLO, James M. , MILGREW, Mark, James , SCHULTZ, Jonathan , MARRAN, David , REARICK, Todd , JOHNSON, Kim L.
Abstract: Methods and apparatus relating to FET arrays including large FET arrays for monitoring chemical and/or biological reactions such as nucleic acid sequencing-by- synthesis reactions. Some methods provided herein relate to improving signal (and also signal-to-noise ratio) from released hydrogen ions during nucleic acid sequencing reactions.
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公开(公告)号：EP4407043A3
公开(公告)日：2024-10-09
申请号：EP24168814.2
申请日：2020-08-14
Applicant: Life Technologies Corporation
Inventor： REED, Mark , WONG, Chiu Tai Andrew , MARRAN, David
IPC: B01J19/00 , B01L3/00 , C12Q1/6806
CPC classification number: C12Q1/6806 , B01J2219/0072220130101 , B01J2219/0060820130101
Abstract: A sequencing system includes an automated sequencing instrument adapted to determine variant calls for one or more extracted polynucleotide samples with a performance of at least 98.5% raw read accuracy and a run time in a range of 5 hours to 14 hours to determine variant calls for 4 extracted polynucleotide samples using a targeted assay with one DNA pool per sample and an average amplicon size in a range of 100 to 120 bases.
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公开(公告)号：EP2436075A1
公开(公告)日：2012-04-04
申请号：EP10780935.2
申请日：2010-05-27
Applicant: Life Technologies Corporation
Inventor： NOBILE, John , ROTH, George, Thomas , REARICK, Todd , SCHULTZ, Jonathan , ROTHBERG, Jonathan, M. , MARRAN, David
CPC classification number: G01N27/301 , B01J2219/00315 , B01J2219/00317 , B01J2219/005 , B01J2219/00653 , B01J2219/00722 , B01L3/502761 , B01L2200/0668 , B01L2300/0636 , B01L2300/0645 , B01L2300/0877 , G01N27/4145
Abstract: The invention is directed to apparatus and methods for delivering multiple reagents to, and monitoring, a plurality of analytical reactions carried out on a large-scale array of electronic sensors under minimal noise conditions. In one aspect, the invention provides method of improving signal-to-noise ratios of output signals from the electronic sensors sensing analytes or reaction byproducts by subtracting an average of output signals measured from neighboring sensors where analyte or reaction byproducts are absent. In other aspects, the invention provides an array of electronic sensors integrated with a microwell array for confining analytes and/or particles for analytical reactions and a method for identifying microwells containing analytes and/or particles by passing a sensor-active reagent over the array and correlating sensor response times to the presence or absence of analytes or particles. Such detection of analyte- or particle-containing microwells may be used as a step in additional noise reduction methods.
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