公开(公告)号：WO2022104224A1

公开(公告)日：2022-05-19

申请号：PCT/US2021/059410

申请日：2021-11-15

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： PODHASKY, Joseph ,  KANE, Nathan ,  REED, Mark

IPC: G01N1/34 ,  C12N15/10 ,  G01N1/40 ,  G01N33/543 ,  G01N35/00

Abstract: A system includes a pipetting system including a 3-axis gantry; a sled mechanism to select a magnetic comb from a set of magnetic combs; a fluorometer; and a set of receptacles to receive welled plates. A method for purifying nucleic acids includes applying a sample to a well of a multi-well plate, selecting a magnetic comb from a set of magnetic combs disposed on a gantry system, collecting magnetic beads using the magnetic comb, collecting nucleic acid using the magnetic beads, and eluting the nucleic acid from the beads.

公开(公告)号：WO2022146708A1

公开(公告)日：2022-07-07

申请号：PCT/US2021/063838

申请日：2021-12-16

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： REED, Mark ,  PODHASKY, Joseph ,  SHANKAR, Shanti

IPC: C12N15/10 ,  C12Q1/6869 ,  G16B30/00 ,  G16B50/00

Abstract: A method for determining a sequence of nucleic acids includes purifying the nucleic acids from a sample with a purification instrument in accordance with a purification plan of a run plan. The purified nucleic acids are disposed in a transfer plate. Disposition of the purified nucleic acids is stored in a transfer file. The method further includes transferring the transfer plate to a sequencing instrument; transferring the transfer file to the sequencing instrument; and sequencing the nucleic acids with the sequencing instrument in accordance with a sequencing plan of the run plan and based on the transfer file.

公开(公告)号：WO2022104272A1

公开(公告)日：2022-05-19

申请号：PCT/US2021/059538

申请日：2021-11-16

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： PODHASKY, Joseph ,  REED, Mark ,  MARRAN, David ,  WONG, Chiu Tai Andrew ,  CHENG, Angie ,  CAO, Ru

IPC: B01L3/00 ,  C12Q1/6869

Abstract: A sequencing system includes an automated sequencing instrument adapted to determine variant calls for one or more extracted polynucleotide samples with a performance of at least 98.5% raw read accuracy and a total instrument run time in a range of 16 hours to 22 hours to determine variant calls when sequencing 6 cfTNA extracted polynucleotide samples using a targeted assay with one nucleic acid pool per sample.

公开(公告)号：EP4271810A1

公开(公告)日：2023-11-08

申请号：EP21854948.3

申请日：2021-12-16

Applicant: Life Technologies Corporation

Inventor： REED, Mark ,  PODHASKY, Joseph ,  SHANKAR, Shanti

IPC: C12N15/10 ,  C12Q1/6869 ,  G16B30/00 ,  G16B50/00

公开(公告)号：EP4244596A1

公开(公告)日：2023-09-20

申请号：EP21827256.5

申请日：2021-11-15

Applicant: Life Technologies Corporation

Inventor： PODHASKY, Joseph ,  KANE, Nathan ,  REED, Mark

IPC: G01N1/34 ,  C12N15/10 ,  G01N1/40 ,  G01N33/543 ,  G01N35/00

公开(公告)号：EP4243986A1

公开(公告)日：2023-09-20

申请号：EP21824191.7

申请日：2021-11-16

Applicant: Life Technologies Corporation

Inventor： PODHASKY, Joseph ,  REED, Mark ,  MARRAN, David ,  WONG, Chiu Tai Andrew ,  CHENG, Angie ,  CAO, Ru

IPC: B01L3/00 ,  C12Q1/6869