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    SYSTEMS AND METHODS FOR SUBSTRATE ENRICHMENT
    2.
    发明申请
    SYSTEMS AND METHODS FOR SUBSTRATE ENRICHMENT 审中-公开
    用于基层增厚的系统和方法

    公开(公告)号：WO2015191877A1

    公开(公告)日：2015-12-17

    申请号：PCT/US2015/035366

    申请日：2015-06-11

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： REED, Brian

    IPC: C12Q1/68

    CPC classification number: C12Q1/6806 ,  C12N15/1013 ,  C12Q1/6844 ,  C12Q2537/163 ,  C12Q2563/159

    Abstract: A method of separating bead substrates includes applying an emulsion to an emulsion-breaking solution. A dispersed phase of the emulsion includes an unbound polynucleotide, a first set of bead substrates and a second set of bead substrates. The unbound polynucleotide includes a segment complementary to a coupling oligonucleotide. The first set of bead substrates includes the coupling oligonucleotide extended to include a segment complementary to a portion of the unbound polynucleotide. The second set of bead substrates includes the coupling oligonucleotide. The emulsion-breaking solution includes an interference probe having a sequence similar to the coupling oligonucleotide or complementary to the coupling oligonucleotide. The method further includes binding beads of the first set of bead substrates to separation substrates and separating unbound beads of the second set of bead substrates from the beads of the first set of bead substrates bound to the separation substrates.

    Abstract translation: 分离珠粒基材的方法包括将乳液施加到破乳溶液中。 乳液的分散相包括未结合的多核苷酸，第一组珠粒基质和第二组珠粒基底。 未结合的多核苷酸包括与偶联寡核苷酸互补的区段。 第一组珠底物包括延伸至包含与未结合多核苷酸的一部分互补的区段的偶联寡核苷酸。 第二组珠底物包括偶联寡核苷酸。 破乳溶液包括具有与偶联寡核苷酸相似的序列或与偶联寡核苷酸互补的序列的干扰探针。 该方法还包括将第一组胎圈衬底的珠粒与分离衬底结合，并将第二组胎圈衬底的未结合的珠粒与与分离衬底结合的第一组胎圈衬底的珠粒分离。

    SYSTEM AND METHODS FOR MAKING AND PROCESSING EMULSIONS
    3.
    发明申请
    SYSTEM AND METHODS FOR MAKING AND PROCESSING EMULSIONS 审中-公开
    制备和处理乳液的系统和方法

    公开(公告)号：WO2012139125A2

    公开(公告)日：2012-10-11

    申请号：PCT/US2012/032786

    申请日：2012-04-09

    Applicant: LIFE TECHNOLOGIES CORPORATION ,  SCHULTZ, Jonathan ,  NOBILE, John ,  REED, Brian ,  THWAR, Prasanna ,  ROSWECH, Todd

    Inventor： SCHULTZ, Jonathan ,  NOBILE, John ,  REED, Brian ,  THWAR, Prasanna ,  ROSWECH, Todd

    IPC: B01F3/08 ,  B01F5/04 ,  B01F13/00 ,  C12Q1/68 ,  B04B5/04 ,  B04B7/02 ,  B01L3/00 ,  B01L7/00

    CPC classification number: B01F5/0602 ,  B01F3/0807 ,  B01F5/0485 ,  B01F13/0059 ,  B01F2215/0037 ,  B01L3/502784 ,  B04B5/0414 ,  B04B7/02

    Abstract: An automated on-touch template bead preparation system is provided and includes a membrane-based emulsion generation subsystems, an emulsion PCR (ePCR) thermocycling plate and subsystem, and a continuous centrifugation emulsion breaking and templated bead collection subsystem. The emulsion generation subsystem provides uniformity in the preparation of an inverse emulsion and may be used to create large or small volume inverse emulsions rapidly and reproducibly. An emulsion-generating device is provided that can supply a continuous stream of an inverse emulsion to a thermocycling subsystem, in automated fashion. The ePCR subsystem can continuously thermocycle an inverse emulsion passed therethrough and includes static temperature zones and a consumable thermocycling plate. The continuous centrifugation subsystem can continuously break a thermally cycled inverse emulsion and collect template beads formed in the aqueous microreactor droplets of the inverse emulsion.

    Abstract translation: 提供了一种自动化的在线模板珠粒制备系统，包括膜基乳液生成子系统，乳液PCR（ePCR）热循环板和子系统，以及连续离心乳液破乳和模板珠收集子系统。 乳液生成子系统在制备反相乳液时提供均匀性，并可用于快速且可再现地产生大体积或小体积的反相乳液。 提供一种乳液生成装置，其能够以自动方式向逆循环子系统提供连续的反相乳液流。 ePCR子系统可以连续热循环通过的反相乳液，并包括静态温度区和可消耗的热循环板。 连续离心子系统可以连续地破坏热循环的反相乳液并收集形成在反相乳液的微量微反应器液滴中的模板珠粒。

    MULTIPLEX NUCLEIC ACID AMPLIFICATION
    4.
    发明申请
    MULTIPLEX NUCLEIC ACID AMPLIFICATION 审中-公开
    多重核酸放大

    公开(公告)号：WO2015191815A1

    公开(公告)日：2015-12-17

    申请号：PCT/US2015/035265

    申请日：2015-06-11

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： JOUN, David ,  LI, Chieh-Yuan ,  REED, Brian ,  OBERGFELL, Craig ,  DRESSMAN, Devin ,  ROSENBAUM, Abraham ,  BENSON, Scott ,  BROKA, Andi ,  GHOSH, Srinka

    IPC: C12Q1/68

    CPC classification number: C12Q1/6806 ,  C12Q1/6846 ,  C12Q2537/143 ,  C12Q2563/149 ,  C12Q2565/537 ,  C12Q2525/191

    Abstract: In some embodiments, the disclosure relates generally to compositions, comprising a single reaction mixture containing a plurality of different populations of discrete supports, and a plurality of different populations of target nucleic acids. The single reaction mixture can contain a first population of beads; a second population of beads; a first population of target nucleic acids, where at least two different target nucleic acids in the first population of target nucleic acids can bind to a bead in the first population of beads; and a second population of target nucleic acids, where at least two different target nucleic acids in the second population of target nucleic acids can bind to a bead in the second population of beads. The single reaction mixture can be employed to monoclonally amplify the first target nucleic acids on the first beads, and monoclonally amplify the second target nucleic acids on the second beads.

    Abstract translation: 在一些实施方案中，本公开一般涉及组合物，其包含含有多个不同群体的离散支持物的单个反应混合物，以及多个不同的靶核酸群体。 单一反应混合物可以含有第一批珠粒; 第二颗珠子; 目标核酸的第一群体，其中靶核酸的第一群体中的至少两种不同靶核酸可以结合珠粒的第一群体中的珠粒; 和靶核酸的第二群体，其中靶核酸的第二群体中的至少两种不同靶核酸可以结合第二群珠体中的珠粒。 单个反应混合物可以用于单层放大第一珠上的第一靶核酸，并单核扩增第二珠上的第二靶核酸。

    SYSTEM AND METHOD FOR EMULSION BREAKING
    5.
    发明申请
    SYSTEM AND METHOD FOR EMULSION BREAKING 审中-公开
    用于乳化断裂的系统和方法

    公开(公告)号：WO2015073332A1

    公开(公告)日：2015-05-21

    申请号：PCT/US2014/064675

    申请日：2014-11-07

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： REED, Brian ,  BALL, James

    IPC: C12Q1/68

    CPC classification number: B01D17/047 ,  B01D17/04 ,  B01D17/041 ,  C07H1/06 ,  C07H21/00 ,  C12Q1/6806 ,  C12Q2527/125 ,  C12Q2527/137 ,  C12Q2563/159

    Abstract: A method of recovering a bead support from an emulsion includes supplying an aqueous surfactant solution into a centrifuge tube; supplying a hydrophobic liquid over the surfactant solution in the centrifuge tube, wherein a ratio of the volume of the aqueous surfactant solution to the volume of the hydrophobic liquid is not greater than 0.5; and applying an emulsion over the hydrophobic liquid while centrifuging, the emulsion comprising a dispersed aqueous phase including the bead support, the emulsion breaking and material of the dispersed phase preferentially partitioning to the surfactant solution.

    Abstract translation: 从乳液中回收珠粒载体的方法包括将表面活性剂水溶液供给到离心管中; 在离心管中的表面活性剂溶液上提供疏水性液体，其中表面活性剂水溶液的体积与疏水性液体的体积之比不大于0.5; 并且在离心时将乳液施加在疏水性液体上，所述乳液包含分散的水相，包括珠粒支持体，破乳剂和分散相的材料优先分配至表面活性剂溶液。

    METHOD FOR TREATING A SEMICONDUCTOR DEVICE
    6.
    发明申请
    METHOD FOR TREATING A SEMICONDUCTOR DEVICE 审中-公开
    用于处理半导体器件的方法

    公开(公告)号：WO2014160962A2

    公开(公告)日：2014-10-02

    申请号：PCT/US2014/032214

    申请日：2014-03-28

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： BALL, James, A. ,  REED, Brian

    IPC: G01N27/414

    CPC classification number: G01N27/4145 ,  B08B3/08 ,  B08B9/08 ,  C11D3/162 ,  C11D3/3418 ,  C11D3/43 ,  C11D7/34 ,  C11D7/5013 ,  C11D7/5022 ,  C11D7/5027

    Abstract: A sensor array includes a plurality of sensors. A sensor of the plurality of sensors has a sensor pad exposed at a surface of the sensor array. A method of treating the sensor array includes exposing at least the sensor pad to a wash solution including sulfonic acid and an organic solvent and rinsing the wash solution from the sensor pad.

    Abstract translation: 传感器阵列包括多个传感器。 多个传感器的传感器具有暴露在传感器阵列的表面处的传感器垫。 处理传感器阵列的方法包括将至少传感器垫暴露于包括磺酸和有机溶剂的洗涤溶液中，并从传感器垫冲洗洗涤溶液。

    METHOD FOR TREATING A SEMICONDUCTOR DEVICE
    9.
    发明公开
    METHOD FOR TREATING A SEMICONDUCTOR DEVICE 有权
    VERFAHREN ZUR BEHANDLUNG EINES HALBLEITERBAUELEMENTS

    公开(公告)号：EP2979083A2

    公开(公告)日：2016-02-03

    申请号：EP14722917.3

    申请日：2014-03-28

    Applicant: Life Technologies Corporation

    Inventor： BALL, James, A. ,  REED, Brian

    IPC: G01N27/414

    CPC classification number: G01N27/4145 ,  B08B3/08 ,  B08B9/08 ,  C11D3/162 ,  C11D3/3418 ,  C11D3/43 ,  C11D7/34 ,  C11D7/5013 ,  C11D7/5022 ,  C11D7/5027

    Abstract: A sensor array includes a plurality of sensors. A sensor of the plurality of sensors has a sensor pad exposed at a surface of the sensor array. A method of treating the sensor array includes exposing at least the sensor pad to a wash solution including sulfonic acid and an organic solvent and rinsing the wash solution from the sensor pad.

    Abstract translation: 传感器阵列包括多个传感器。 多个传感器的传感器具有在传感器阵列的表面上暴露的传感器垫。 处理传感器阵列的方法包括将至少传感器垫暴露于包括磺酸和有机溶剂的洗涤溶液中并从传感器垫冲洗洗涤溶液。

    SYSTEM AND METHOD FOR EMULSION BREAKING
    10.
    发明公开
    SYSTEM AND METHOD FOR EMULSION BREAKING 审中-公开

    公开(公告)号：EP3839062A1

    公开(公告)日：2021-06-23

    申请号：EP21157133.6

    申请日：2014-11-07

    Applicant: Life Technologies Corporation

    Inventor： REED, Brian ,  BALL, James

    IPC: C12Q1/68 ,  C12Q1/6806

    Abstract: A method ofrecovering a bead support from an emulsion in-cludes supplying an aqueous surfactant solution into a centrifuge tube; sup -plying a hydrophobic liquid over the surfactant solution in the centrifuge tube, wherein a ratio of the volume of the aqueous surfactant solution to the volume of the hydrophobic liquid is not greater than 0.5; and applying an emulsion over the hydrophobic liquid while centrifuging, the emulsion com-prising a dispersed aqueous phase including the bead support, the emulsion breaking and material of the dispersed phase preferentially partitioning to the surfactant solution.

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