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    ASYMMETRIC RHODAMINE DYE AND USE THEREOF IN BIOLOGICAL ASSAYS
    1.
    发明申请
    ASYMMETRIC RHODAMINE DYE AND USE THEREOF IN BIOLOGICAL ASSAYS 审中-公开

    公开(公告)号：WO2020132607A1

    公开(公告)日：2020-06-25

    申请号：PCT/US2019/068111

    申请日：2019-12-20

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： EVANS, Brian ,  BENSON, Scott

    IPC: C09B11/24 ,  C07H21/00 ,  C12N15/65 ,  G01N33/58

    Abstract: The present disclosure relates to N-protected NH-rhodamine dyes and their use in nucleic acid detection. In particular, the disclosure relates to methods of making N-protected NH-rhodamine dyes, and methods of use of N-protected NH-rhodamine dyes (e.g., human identification). Certain dyes provided herein have unique spectral properties that complement those in existing dye sets and can be used to expand the number of reporter dyes that can be included for HID applications and other biological assays. Those fluorescent compounds are useful to label synthetic oligonucleotides. Formula (I).

    ASYMMETRIC RHODAMINE DYE AND USE THEREOF IN BIOLOGICAL ASSAYS
    5.
    发明申请
    ASYMMETRIC RHODAMINE DYE AND USE THEREOF IN BIOLOGICAL ASSAYS 审中-公开

    公开(公告)号：WO2020132607A9

    公开(公告)日：2020-06-25

    申请号：PCT/US2019/068111

    申请日：2019-12-20

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： EVANS, Brian ,  BENSON, Scott

    IPC: C09B11/24 ,  C07H21/00 ,  C12N15/65 ,  G01N33/58

    Abstract: The present disclosure relates to N-protected NH-rhodamine dyes and their use in nucleic acid detection. In particular, the disclosure relates to methods of making N-protected NH-rhodamine dyes, and methods of use of N-protected NH-rhodamine dyes (e.g., human identification). Certain dyes provided herein have unique spectral properties that complement those in existing dye sets and can be used to expand the number of reporter dyes that can be included for HID applications and other biological assays. Those fluorescent compounds are useful to label synthetic oligonucleotides. Formula (I).

    SYSTEMS AND METHODS FOR BIOLOGICAL ANALYSIS
    6.
    发明申请
    SYSTEMS AND METHODS FOR BIOLOGICAL ANALYSIS 审中-公开

    公开(公告)号：WO2022020729A2

    公开(公告)日：2022-01-27

    申请号：PCT/US2021/042997

    申请日：2021-07-23

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： FREUDENTHAL, Jacob ,  BENSON, Scott

    IPC: G01N21/64 ,  C12Q1/686 ,  C12Q1/6851 ,  G01J3/4406 ,  G01N2021/6421 ,  G01N2021/6432 ,  G01N2021/6441 ,  G01N21/274 ,  G01N21/6428 ,  G01N21/645 ,  G01N21/6452 ,  G01N21/6456

    Abstract: 1. A system (1000), comprising: a radiant source (101) characterized by an average excitation wavelength; a sample (110) disposed to receive radiation from the radiant source, the sample comprising:a first dye;a second dye; and a detector (115) configured to measure emissions from the sample; a first emission spectral element (121a) characterized by a first average emission wavelength; a second emission spectral element (121b) characterized by a second average emission wavelength that is different than the first average emission wavelength; at least one processor (130) comprising at least one memory including instructions to: illuminate the sample with the radiant source and, in response, (1) measure emissions from the sample using the detector and the first emission spectral element and (2) measure emissions from the sample using the detector and the second emission spectral element. A method corresponding to the above system.

    MULTIPLEX NUCLEIC ACID AMPLIFICATION
    9.
    发明申请
    MULTIPLEX NUCLEIC ACID AMPLIFICATION 审中-公开
    多重核酸放大

    公开(公告)号：WO2015191815A1

    公开(公告)日：2015-12-17

    申请号：PCT/US2015/035265

    申请日：2015-06-11

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： JOUN, David ,  LI, Chieh-Yuan ,  REED, Brian ,  OBERGFELL, Craig ,  DRESSMAN, Devin ,  ROSENBAUM, Abraham ,  BENSON, Scott ,  BROKA, Andi ,  GHOSH, Srinka

    IPC: C12Q1/68

    CPC classification number: C12Q1/6806 ,  C12Q1/6846 ,  C12Q2537/143 ,  C12Q2563/149 ,  C12Q2565/537 ,  C12Q2525/191

    Abstract: In some embodiments, the disclosure relates generally to compositions, comprising a single reaction mixture containing a plurality of different populations of discrete supports, and a plurality of different populations of target nucleic acids. The single reaction mixture can contain a first population of beads; a second population of beads; a first population of target nucleic acids, where at least two different target nucleic acids in the first population of target nucleic acids can bind to a bead in the first population of beads; and a second population of target nucleic acids, where at least two different target nucleic acids in the second population of target nucleic acids can bind to a bead in the second population of beads. The single reaction mixture can be employed to monoclonally amplify the first target nucleic acids on the first beads, and monoclonally amplify the second target nucleic acids on the second beads.

    Abstract translation: 在一些实施方案中，本公开一般涉及组合物，其包含含有多个不同群体的离散支持物的单个反应混合物，以及多个不同的靶核酸群体。 单一反应混合物可以含有第一批珠粒; 第二颗珠子; 目标核酸的第一群体，其中靶核酸的第一群体中的至少两种不同靶核酸可以结合珠粒的第一群体中的珠粒; 和靶核酸的第二群体，其中靶核酸的第二群体中的至少两种不同靶核酸可以结合第二群珠体中的珠粒。 单个反应混合物可以用于单层放大第一珠上的第一靶核酸，并单核扩增第二珠上的第二靶核酸。

    METHODS OF TREATING METAL OXIDE SURFACES AND SURFACES PRODUCED THEREBY
    10.
    发明申请
    METHODS OF TREATING METAL OXIDE SURFACES AND SURFACES PRODUCED THEREBY 审中-公开
    处理金属氧化物表面和生产的表面的方法

    公开(公告)号：WO2011106451A1

    公开(公告)日：2011-09-01

    申请号：PCT/US2011/025954

    申请日：2011-02-23

    Applicant: LIFE TECHNOLOGIES CORPORATION ,  FOSTER, Evan ,  REED, Jessica ,  SHEPHERD, Heather ,  INMAN, Christina ,  BENSON, Scott

    Inventor： FOSTER, Evan ,  REED, Jessica ,  SHEPHERD, Heather ,  INMAN, Christina ,  BENSON, Scott

    IPC: C12Q1/68 ,  B01J19/00 ,  G01N33/543 ,  G01N33/552

    CPC classification number: C12Q1/6834 ,  B01J19/0046 ,  B01J2219/00612 ,  B01J2219/00635 ,  B01J2219/00637 ,  B01J2219/00648 ,  B01J2219/00722 ,  B01L3/502707

    Abstract: Methods for activating and/or reactivating a metal oxide surface for deposition, immobilizing, and/or growing a biological sample (e.g., an oligonucleotide, a polynucleotide, functionalized particle, a polymer, etc.) thereon are disclosed. The metal oxide surface (e.g., zirconium oxide) can be activated and/or reactivated by exposing the metal oxide surface to various activation and/or treatment protocols capable of enhancing a slide's activity relative to the activity of an untreated slide. The activation and/or treatment protocol can include subjecting the slide (or at least the metal oxide layer) to an oxygen plasma treatment and/or a peroxide solution for an amount of time sufficient to activate and/or reactivate the metal oxide surface. Activated sequencing slides capable of incorporation, for example, into various embodiments of a flow cell for use in NGS platforms are also disclosed herein.

    Abstract translation: 公开了用于活化和/或重新激活用于在其上沉积，固定和/或生长生物样品（例如寡核苷酸，多核苷酸，官能化颗粒，聚合物等）的金属氧化物表面的方法。 金属氧化物表面（例如，氧化锆）可以通过将金属氧化物表面暴露于能够相对于未处理载玻片的活性增强载玻片的活性的各种活化和/或处理方案而被活化和/或再活化。 活化和/或处理方案可以包括使载玻片（或至少金属氧化物层）经历氧等离子体处理和/或过氧化物溶液足以活化和/或重新活化金属氧化物表面的时间量。 本文还公开了能够并入例如用于NGS平台的流动池的各种实施方案中的活化测序载玻片。

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