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    RAPID AMPLIFICATION OF NUCLEIC ACIDS
    2.
    发明申请
    RAPID AMPLIFICATION OF NUCLEIC ACIDS 审中-公开
    快速扩增核酸

    公开(公告)号：WO2018071522A1

    公开(公告)日：2018-04-19

    申请号：PCT/US2017/056113

    申请日：2017-10-11

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： LIGHT, David ,  CHEN, Shann-Ching ,  DHINGRA, Dalia ,  TOM, Warren ,  ROSENBAUM, Abraham ,  SEEGER, Collyn ,  THWAR, Prasanna

    IPC: C12Q1/68 ,  C12Q1/6853 ,  C12Q1/6865

    CPC classification number: C12Q1/6846 ,  C12Q1/6853 ,  C12Q1/6865 ,  C12Q2525/149 ,  C12Q2563/149 ,  C12Q2565/537 ,  C12Q2525/191 ,  C12Q2525/161

    Abstract: In some embodiments, the disclosure relates generally to methods, and related compositions, systems, kits and apparatuses, for conducting a stream-lined nucleic acid library preparation workflow that generally includes binding a polynucleotide (from an initial nucleic acid sample) directly to capture primers that are immobilized on a support (e.g., beads or flowcell), and amplifying the bound polynucleotide to produce a support that carries a substantially monoclonal population of the polynucleotide (or a portion thereof). The support that carries the substantially monoclonal population of the polynucleotide can be generated, using the present teachings, without performing a separate adaptor-joining step and is ready for sequencing.

    Abstract translation: 在一些实施方案中，本公开内容一般涉及用于进行流线型核酸文库制备工作流程的方法和相关组合物，系统，试剂盒和设备，其通常包括结合多核苷酸（来自初始 核酸样品）直接捕获固定在载体（例如珠或流动池）上的引物，并扩增结合的多核苷酸以产生携带基本上单克隆群体的多核苷酸（或其一部分）的载体。 使用本发明的教导，可以产生携带基本上单克隆的多核苷酸群体的支持物，而无需执行单独的衔接子连接步骤并准备好测序。

    METHODS AND COMPOSITIONS FOR NUCLEIC ACID AMPLIFICATION
    3.
    发明申请
    METHODS AND COMPOSITIONS FOR NUCLEIC ACID AMPLIFICATION 审中-公开
    核酸扩增的方法和组合物

    公开(公告)号：WO2017214561A1

    公开(公告)日：2017-12-14

    申请号：PCT/US2017/036842

    申请日：2017-06-09

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： YU, Hua ,  NIKIFOROV, Theo ,  ROSENBAUM, Abraham

    IPC: C12N15/09 ,  C12P19/34 ,  C12Q1/68

    Abstract: In some embodiments, the disclosure relates generally to methods, as well as related compositions and kits for recombinase-mediated nucleic acid amplification, such as recombinase-polymerase amplification (RPA), of a nucleic acid template using at least one blocked primer that contains a 5' domain, at least one nucleotide that is cleavable by an RNase H enzyme, a 3' domain, wherein the primer is not extendable by a polymerase, and wherein the 3' domain has a length of 7-100 nucleotides, for example 10-30 nucleotides. These methods and the use of a blocked primer reduce or eliminate non-specific amplification products, such as primer dimers, which are generated in RPA reactions.

    Abstract translation: 在一些实施方案中，本公开内容一般涉及使用核酸模板的核酸模板的用于重组酶介导的核酸扩增例如重组酶 - 聚合酶扩增（RPA）的方法以及相关组合物和试剂盒 至少一种含有5'结构域的封闭引物，至少一个可被RNase H酶切割的核苷酸，3'结构域，其中引物不能被聚合酶延伸，并且其中3'结构域的长度为 7-100个核苷酸，例如10-30个核苷酸。 这些方法和封闭引物的使用减少或消除在RPA反应中产生的非特异性扩增产物，例如引物二聚体。

    MULTIPLEX NUCLEIC ACID AMPLIFICATION
    4.
    发明申请
    MULTIPLEX NUCLEIC ACID AMPLIFICATION 审中-公开
    多重核酸放大

    公开(公告)号：WO2015191815A1

    公开(公告)日：2015-12-17

    申请号：PCT/US2015/035265

    申请日：2015-06-11

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： JOUN, David ,  LI, Chieh-Yuan ,  REED, Brian ,  OBERGFELL, Craig ,  DRESSMAN, Devin ,  ROSENBAUM, Abraham ,  BENSON, Scott ,  BROKA, Andi ,  GHOSH, Srinka

    IPC: C12Q1/68

    CPC classification number: C12Q1/6806 ,  C12Q1/6846 ,  C12Q2537/143 ,  C12Q2563/149 ,  C12Q2565/537 ,  C12Q2525/191

    Abstract: In some embodiments, the disclosure relates generally to compositions, comprising a single reaction mixture containing a plurality of different populations of discrete supports, and a plurality of different populations of target nucleic acids. The single reaction mixture can contain a first population of beads; a second population of beads; a first population of target nucleic acids, where at least two different target nucleic acids in the first population of target nucleic acids can bind to a bead in the first population of beads; and a second population of target nucleic acids, where at least two different target nucleic acids in the second population of target nucleic acids can bind to a bead in the second population of beads. The single reaction mixture can be employed to monoclonally amplify the first target nucleic acids on the first beads, and monoclonally amplify the second target nucleic acids on the second beads.

    Abstract translation: 在一些实施方案中，本公开一般涉及组合物，其包含含有多个不同群体的离散支持物的单个反应混合物，以及多个不同的靶核酸群体。 单一反应混合物可以含有第一批珠粒; 第二颗珠子; 目标核酸的第一群体，其中靶核酸的第一群体中的至少两种不同靶核酸可以结合珠粒的第一群体中的珠粒; 和靶核酸的第二群体，其中靶核酸的第二群体中的至少两种不同靶核酸可以结合第二群珠体中的珠粒。 单个反应混合物可以用于单层放大第一珠上的第一靶核酸，并单核扩增第二珠上的第二靶核酸。

    MULTIPLEX NUCLEIC ACID AMPLIFICATION
    7.
    发明公开
    MULTIPLEX NUCLEIC ACID AMPLIFICATION 审中-公开
    多重NUKLEINSÄUREAMPLIFIKATION

    公开(公告)号：EP3155127A1

    公开(公告)日：2017-04-19

    申请号：EP15731786.8

    申请日：2015-06-11

    Applicant: Life Technologies Corporation

    Inventor： JOUN, David ,  LI, Chieh-Yuan ,  REED, Brian ,  OBERGFELL, Craig ,  DRESSMAN, Devin ,  ROSENBAUM, Abraham ,  BENSON, Scott ,  BROKA, Andi ,  GHOSH, Srinka

    IPC: C12Q1/68

    Abstract: In some embodiments, the disclosure relates generally to compositions, comprising a single reaction mixture containing a plurality of different populations of discrete supports, and a plurality of different populations of target nucleic acids. The single reaction mixture can contain a first population of beads; a second population of beads; a first population of target nucleic acids, where at least two different target nucleic acids in the first population of target nucleic acids can bind to a bead in the first population of beads; and a second population of target nucleic acids, where at least two different target nucleic acids in the second population of target nucleic acids can bind to a bead in the second population of beads. The single reaction mixture can be employed to monoclonally amplify the first target nucleic acids on the first beads, and monoclonally amplify the second target nucleic acids on the second beads.

    Abstract translation: 在一些实施方案中，本公开一般涉及组合物，其包含含有多个不同群体的离散支持物的单个反应混合物，以及多个不同的靶核酸群体。 单一反应混合物可以含有第一批珠粒; 第二颗珠子; 目标核酸的第一群体，其中靶核酸的第一群体中的至少两种不同靶核酸可以结合珠粒的第一群体中的珠粒; 和靶核酸的第二群体，其中靶核酸的第二群体中的至少两种不同靶核酸可以结合第二群珠体中的珠粒。 单个反应混合物可以用于单层放大第一珠上的第一靶核酸，并单核扩增第二珠上的第二靶核酸。

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