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    NUCLEIC ACID AMPLIFICATION
    1.
    发明申请
    NUCLEIC ACID AMPLIFICATION 审中-公开
    核酸放大

    公开(公告)号：WO2014043143A1

    公开(公告)日：2014-03-20

    申请号：PCT/US2013/059093

    申请日：2013-09-10

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： LI, Chieh-Yuan ,  RUFF, David ,  CHEN, Shiaw-Min ,  O'NEIL, Jennifer ,  KASINSKAS, Rachel ,  ROTHBERG, Jonathan ,  HINZ, Wolfgang

    IPC: C12Q1/68

    CPC classification number: C12Q1/6869 ,  C12Q2521/507 ,  C12Q2527/119 ,  C12Q2535/122 ,  C12Q2565/537 ,  C12Q2565/607

    Abstract: In some embodiments, the present teachings provide methods for nucleic acid amplification, comprising forming a reaction mixture, and subjecting the reaction mixture to conditions suitable for nucleic acid amplification. In some embodiments, methods for nucleic acid amplification include subjecting the nucleic acid to be amplified to partially denaturing conditions. In some embodiments, methods for nucleic acid amplification include amplifying without fully denaturing the nucleic acid that is amplified. In some embodiments, the methods for nucleic acid amplification employ an enzyme that catalyzes homologous recombination and a polymerase. In some embodiments, methods for nucleic acid amplification can be conducted in a single reaction vessel. In some embodiments, methods for nucleic acid amplification can be conducted in a single continuous liquid phase of a reaction mixture, without need for compartmentalization of the reaction mixture or immobilization of reaction components. In some embodiments, methods for nucleic acid amplification comprise a amplifying at least one polynucleotide onto a surface under isothermal amplification conditions, optionally in the presence of a polymer. The polymer can include a sieving agent and/or a diffusion-reducing agent.

    Abstract translation: 在一些实施方案中，本发明提供核酸扩增方法，包括形成反应混合物，并使反应混合物经受适合于核酸扩增的条件。 在一些实施方案中，用于核酸扩增的方法包括将待扩增的核酸进行部分变性的条件。 在一些实施方案中，用于核酸扩增的方法包括扩增而不完全变性扩增的核酸。 在一些实施方案中，用于核酸扩增的方法使用催化同源重组的酶和聚合酶。 在一些实施方案中，用于核酸扩增的方法可以在单个反应容器中进行。 在一些实施方案中，用于核酸扩增的方法可以在反应混合物的单个连续液相中进行，而不需要分隔反应混合物或固定反应组分。 在一些实施方案中，用于核酸扩增的方法包括在等温扩增条件下任选地在聚合物存在下在表面上扩增至少一种多核苷酸。 聚合物可以包括筛分剂和/或扩散还原剂。

    MODIFIED PROTEINS AND METHODS OF MAKING AND USING SAME
    2.
    发明申请
    MODIFIED PROTEINS AND METHODS OF MAKING AND USING SAME 审中-公开
    修饰的蛋白质及其制备和使用方法

    公开(公告)号：WO2011106634A2

    公开(公告)日：2011-09-01

    申请号：PCT/US2011/026228

    申请日：2011-02-25

    Applicant: LIFE TECHNOLOGIES CORPORATION ,  DAVIDSON, John, F. ,  HINZ, Wolfgang ,  ROTHBERG, Jonathan ,  WHITAKER, Richard

    Inventor： DAVIDSON, John, F. ,  HINZ, Wolfgang ,  ROTHBERG, Jonathan ,  WHITAKER, Richard

    IPC: C12Q1/68 ,  C12Q1/48 ,  C12N9/12

    CPC classification number: C12N9/1252

    Abstract: Methods, compositions, systems, apparatuses and kits comprising modified proteins, particularly modified nucleic acid-binding proteins with altered buffering properties are provided. For example, in some embodiments, methods of forming modified proteins including one or more amino acid modifications to achieve desired pKa values are described. Furthermore, the invention provides methods for using such modified proteins in ion-producing reactions, such as ion-based nucleic acid sequencing reactions.

    Abstract translation: 提供了包含修饰的蛋白质，特别是具有改变的缓冲性质的修饰的核酸结合蛋白的方法，组合物，系统，装置和试剂盒。 例如，在一些实施方案中，描述了形成包含一个或多个氨基酸修饰的修饰蛋白以实现期望的pKa值的方法。 此外，本发明提供了在离子生成反应中使用这些修饰蛋白的方法，如基于离子的核酸测序反应。

    NUCLEIC ACID AMPLIFICATION
    3.
    发明申请
    NUCLEIC ACID AMPLIFICATION 审中-公开
    核酸放大

    公开(公告)号：WO2013158313A1

    公开(公告)日：2013-10-24

    申请号：PCT/US2013/032598

    申请日：2013-03-15

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： LI, Chieh-Yuan ,  RUFF, David ,  CHEN, Shiaw-Min ,  O'NEIL, Jennifer ,  KASINSKAS, Rachel ,  ROTHBERG, Jonathan

    IPC: C12Q1/68

    CPC classification number: C12Q1/6806 ,  C12Q2521/507 ,  C12Q2527/146 ,  C12Q2535/122 ,  C12Q2563/159 ,  C12Q2565/537 ,  C12Q2565/607

    Abstract: In some embodiments, the present teachings provide methods for nucleic acid amplification, comprising forming an reaction mixture, and subjecting the reaction mixture to conditions suitable for nucleic acid amplification. In some embodiments, methods for nucleic acid amplification include subjecting the nucleic acid to be amplified to partially denaturing conditions. In some embodiments, methods for nucleic acid amplification include amplifying without fully denaturing the nucleic acid that is amplified. In some embodiments, the methods for nucleic acid amplification employ an enzyme that catalyzes homologous recombination and a polymerase. In some embodiments, methods for nucleic acid amplification can be conducted in a single reaction vessel. In some embodiments, methods for nucleic acid amplification can be conducted in a single continuous liquid phase of an reaction mixture, without need for compartmentalization of the reaction mixture or immobilization of reaction components. In some embodiments, methods for nucleic acid amplification comprise a amplifying at least one polynucleotide onto a surface under isothermal amplification conditions, optionally in the presence of a polymer. The polymer can include a sieving agent and/or a diffusion-reducing agent.

    Abstract translation: 在一些实施方案中，本发明提供核酸扩增方法，包括形成反应混合物，并使反应混合物经受适合于核酸扩增的条件。 在一些实施方案中，用于核酸扩增的方法包括将待扩增的核酸进行部分变性的条件。 在一些实施方案中，用于核酸扩增的方法包括扩增而不完全变性所扩增的核酸。 在一些实施方案中，用于核酸扩增的方法使用催化同源重组的酶和聚合酶。 在一些实施方案中，用于核酸扩增的方法可以在单个反应容器中进行。 在一些实施方案中，用于核酸扩增的方法可以在反应混合物的单个连续液相中进行，而不需要分隔反应混合物或固定反应组分。 在一些实施方案中，用于核酸扩增的方法包括在等温扩增条件下任选在聚合物存在下在表面上扩增至少一种多核苷酸。 聚合物可以包括筛分剂和/或扩散还原剂。

    CHEMICAL SENSOR ARRAY HAVING MULTIPLE SENSORS PER WELL
    4.
    发明申请
    CHEMICAL SENSOR ARRAY HAVING MULTIPLE SENSORS PER WELL 审中-公开
    具有多个传感器的化学传感器阵列

    公开(公告)号：WO2014200775A1

    公开(公告)日：2014-12-18

    申请号：PCT/US2014/040923

    申请日：2014-06-04

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： ROTHBERG, Jonathan ,  FIFE, Keith ,  BUSTILLO, James ,  OWENS, Jordan

    IPC: G01N27/414

    CPC classification number: G01N27/4145 ,  C12Q1/6874 ,  G01N27/414 ,  H01L29/66825

    Abstract: The device includes a material defining a reaction region. The device includes a plurality of chemically-sensitive field effect transistors (chemFET) each having a common floating gate (370) in communication with the reaction region. The device also includes a circuit to obtain respective output signals from the chemically- sensitive field effect transistors indicating an analyte within the reaction region.

    Abstract translation: 该装置包括限定反应区域的材料。 该器件包括多个化学敏感的场效应晶体管（chemFET），每个具有与反应区域连通的公共浮动栅极（370）。 该器件还包括一个电路，以从化学敏感的场效应晶体管获得指示反应区域内的分析物的各自的输出信号。

    MODIFIED PROTEINS AND METHODS OF MAKING AND USING SAME
    8.
    发明申请
    MODIFIED PROTEINS AND METHODS OF MAKING AND USING SAME 审中-公开
    改良蛋白质及其制备方法和使用方法

    公开(公告)号：WO2011106770A2

    公开(公告)日：2011-09-01

    申请号：PCT/US2011/026468

    申请日：2011-02-28

    Applicant: LIFE TECHNOLOGIES CORPORATION ,  DAVIDSON, John, F. ,  HINZ, Wolfgang ,  ROTHBERG, Jonathan ,  WHITAKER, Richard

    Inventor： DAVIDSON, John, F. ,  HINZ, Wolfgang ,  ROTHBERG, Jonathan ,  WHITAKER, Richard

    IPC: C12Q1/68 ,  C12Q1/48 ,  C12N9/12

    CPC classification number: C12N9/1252

    Abstract: Methods, compositions, systems, apparatuses and kits comprising modified proteins, particularly modified nucleic acid-binding proteins with altered buffering properties are provided. For example, in some embodiments, methods of forming modified proteins including one or more amino acid modifications to achieve desired pKa values are described. Furthermore, the invention provides methods for using such modified proteins in ion-producing reactions, such as ion-based nucleic acid sequencing reactions.

    Abstract translation: 提供了包含修饰的蛋白质，特别是具有改变的缓冲性质的经修饰的核酸结合蛋白质的方法，组合物，系统，装置和试剂盒。 例如，在一些实施方案中，描述了形成包含一个或多个氨基酸修饰以获得所需pKa值的修饰蛋白质的方法。 此外，本发明提供了在离子产生反应中使用这些修饰蛋白质的方法，例如基于离子的核酸测序反应。

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