公开(公告)号：WO2017058810A2

公开(公告)日：2017-04-06

申请号：PCT/US2016/053994

申请日：2016-09-27

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： MAZUR, Daniel ,  VANDER HORN, Peter ,  TOZER, Eileen ,  CHEN, Sihong ,  LUO, Guobin ,  SHIRLEY, Joshua ,  HEINEMANN, Kevin

IPC: C12Q1/68 ,  C12N9/12

CPC classification number: C12N9/1252 ,  C12Q1/686 ,  C12Y207/07007 ,  C12Q2521/101

Abstract: The present disclosure provides compositions, methods, kits, systems and apparatus that are useful for nucleic acid polymerization. In particular, recombinant polymerases and biologically active fragments thereof are provided that allow for nucleic acid amplification. In some aspects, the disclosure provides recombinant polymerases that yield lower systematic error rates and/or improved accuracy, when used in sequencing by synthesis reactions as compared to a control polymerase. In one aspect, the disclosure relates to recombinant polymerases useful for nucleic acid sequencing, genotyping, copy number variation analysis, paired-end sequencing and other forms of genetic analysis. In another aspect, the recombinant polymerases are useful for the amplification of nucleic acid templates during PCR, emPCR, isothermal amplification, recombinase polymerase amplification, rolling circle amplification, strand displacement amplification and proximity ligation amplification. In some aspects, the disclosure relates to recombinant polymerases useful for the generation of nucleic acid libraries and/or nucleic acid templates.

Abstract translation: 本公开提供了可用于核酸聚合的组合物，方法，试剂盒，系统和装置。 特别地，提供允许核酸扩增的重组聚合酶及其生物活性片段。 在一些方面，当与对照聚合酶相比时，通过合成反应进行测序时，本公开提供了产生较低系统误差率和/或改善的准确度的重组聚合酶。 一方面，本公开涉及可用于核酸测序，基因分型，拷贝数变异分析，配对末端测序和其他形式遗传分析的重组聚合酶。 另一方面，重组聚合酶可用于在PCR，emPCR，等温扩增，重组酶聚合酶扩增，滚环扩增，链置换扩增和接近连接扩增期间扩增核酸模板。 在一些方面，本公开涉及可用于产生核酸文库和/或核酸模板的重组聚合酶。

公开(公告)号：EP4141126A1

公开(公告)日：2023-03-01

申请号：EP22185494.6

申请日：2016-09-27

Applicant: Life Technologies Corporation

Inventor： MAZUR, Daniel ,  VANDER HORN, Peter ,  TOZER, Eileen ,  CHEN, Sihong ,  LUO, Guobin ,  SHIRLEY, Joshua ,  HEINEMANN, Kevin

IPC: C12Q1/68 ,  C12N9/12

Abstract: The present disclosure provides compositions, methods, kits, systems and apparatus that are useful for nucleic acid polymerization. In particular, recombinant polymerases and biologically active fragments thereof are provided that allow for nucleic acid amplification. In some aspects, the disclosure provides recombinant polymerases that yield lower systematic error rates and/or improved accuracy, when used in sequencing by synthesis reactions as compared to a control polymerase. In one aspect, the disclosure relates to recombinant polymerases useful for nucleic acid sequencing, genotyping, copy number variation analysis, paired-end sequencing and other forms of genetic analysis. In another aspect, the recombinant polymerases are useful for the amplification of nucleic acid templates during PCR, emPCR, isothermal amplification, recombinase polymerase amplification, rolling circle amplification, strand displacement amplification and proximity ligation amplification. In some aspects, the disclosure relates to recombinant polymerases useful for the generation of nucleic acid libraries and/or nucleic acid templates.

公开(公告)号：EP3356557A2

公开(公告)日：2018-08-08

申请号：EP16794093.1

申请日：2016-09-27

Applicant: Life Technologies Corporation

Inventor： MAZUR, Daniel ,  VANDER HORN, Peter ,  TOZER, Eileen ,  CHEN, Sihong ,  LUO, Guobin ,  SHIRLEY, Joshua ,  HEINEMANN, Kevin

IPC: C12Q1/68 ,  C12N9/12

CPC classification number: C12N9/1252 ,  C12Q1/686 ,  C12Y207/07007 ,  C12Q2521/101

Abstract: The present disclosure provides compositions, methods, kits, systems and apparatus that are useful for nucleic acid polymerization. In particular, recombinant polymerases and biologically active fragments thereof are provided that allow for nucleic acid amplification. In some aspects, the disclosure provides recombinant polymerases that yield lower systematic error rates and/or improved accuracy, when used in sequencing by synthesis reactions as compared to a control polymerase. In one aspect, the disclosure relates to recombinant polymerases useful for nucleic acid sequencing, genotyping, copy number variation analysis, paired-end sequencing and other forms of genetic analysis. In another aspect, the recombinant polymerases are useful for the amplification of nucleic acid templates during PCR, emPCR, isothermal amplification, recombinase polymerase amplification, rolling circle amplification, strand displacement amplification and proximity ligation amplification. In some aspects, the disclosure relates to recombinant polymerases useful for the generation of nucleic acid libraries and/or nucleic acid templates.