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    POLYMERASE COMPOSITIONS AND KITS, AND METHODS OF USING AND MAKING THE SAME
    1.
    发明申请
    POLYMERASE COMPOSITIONS AND KITS, AND METHODS OF USING AND MAKING THE SAME 审中-公开
    聚合物组合物和组合物，以及使用和制备它们的方法

    公开(公告)号：WO2017058810A2

    公开(公告)日：2017-04-06

    申请号：PCT/US2016/053994

    申请日：2016-09-27

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： MAZUR, Daniel ,  VANDER HORN, Peter ,  TOZER, Eileen ,  CHEN, Sihong ,  LUO, Guobin ,  SHIRLEY, Joshua ,  HEINEMANN, Kevin

    IPC: C12Q1/68 ,  C12N9/12

    CPC classification number: C12N9/1252 ,  C12Q1/686 ,  C12Y207/07007 ,  C12Q2521/101

    Abstract: The present disclosure provides compositions, methods, kits, systems and apparatus that are useful for nucleic acid polymerization. In particular, recombinant polymerases and biologically active fragments thereof are provided that allow for nucleic acid amplification. In some aspects, the disclosure provides recombinant polymerases that yield lower systematic error rates and/or improved accuracy, when used in sequencing by synthesis reactions as compared to a control polymerase. In one aspect, the disclosure relates to recombinant polymerases useful for nucleic acid sequencing, genotyping, copy number variation analysis, paired-end sequencing and other forms of genetic analysis. In another aspect, the recombinant polymerases are useful for the amplification of nucleic acid templates during PCR, emPCR, isothermal amplification, recombinase polymerase amplification, rolling circle amplification, strand displacement amplification and proximity ligation amplification. In some aspects, the disclosure relates to recombinant polymerases useful for the generation of nucleic acid libraries and/or nucleic acid templates.

    Abstract translation: 本公开提供了可用于核酸聚合的组合物，方法，试剂盒，系统和装置。 特别地，提供允许核酸扩增的重组聚合酶及其生物活性片段。 在一些方面，当与对照聚合酶相比时，通过合成反应进行测序时，本公开提供了产生较低系统误差率和/或改善的准确度的重组聚合酶。 一方面，本公开涉及可用于核酸测序，基因分型，拷贝数变异分析，配对末端测序和其他形式遗传分析的重组聚合酶。 另一方面，重组聚合酶可用于在PCR，emPCR，等温扩增，重组酶聚合酶扩增，滚环扩增，链置换扩增和接近连接扩增期间扩增核酸模板。 在一些方面，本公开涉及可用于产生核酸文库和/或核酸模板的重组聚合酶。

    POLYMERASE COMPOSITIONS, METHODS OF MAKING AND USING SAME
    2.
    发明申请
    POLYMERASE COMPOSITIONS, METHODS OF MAKING AND USING SAME 审中-公开
    聚合酶组合物，其制备和使用方法

    公开(公告)号：WO2013023176A2

    公开(公告)日：2013-02-14

    申请号：PCT/US2012/050423

    申请日：2012-08-10

    Applicant: LIFE TECHNOLOGIES CORPORATION ,  VANDER HORN, Peter ,  NIKIFOROV, Theo ,  LUO, Guobin ,  LANDES, Mindy ,  MAZUR, Daniel ,  TOZER, Eileen ,  LINCECUM, Tommie

    Inventor： VANDER HORN, Peter ,  NIKIFOROV, Theo ,  LUO, Guobin ,  LANDES, Mindy ,  MAZUR, Daniel ,  TOZER, Eileen ,  LINCECUM, Tommie

    IPC: C12Q1/68

    CPC classification number: C12N9/1252 ,  C12Q1/6846 ,  C12Q1/686 ,  C12Y207/07007

    Abstract: The present disclosure provides compositions, methods, kits, systems and apparatus that are useful for nucleic acid polymerization. In particular, modified polymerases and biologically active fragment thereof are provided that allow for nucleic acid amplification. In one aspect, the disclosure relates to modified polymerases useful for nucleic acid sequencing, genotyping, copy number variation analysis, paired-end sequencing and other forms of genetic analysis. In some aspects, the disclosure relates to modified polymerases useful for the generation of nucleic acid libraries or nucleic acid templates for use in various downstream processes. In some aspects, the disclosure relates to the identification of homologous amino acid mutations that can be transferred across classes or families of polymerases to provide novel polymerases with altered catalytic properties. In some aspects, the disclosure provides modified polymerases having enhanced catalytic properties as compared to a reference polymerase.

    Abstract translation: 本公开提供了用于核酸聚合的组合物，方法，试剂盒，系统和装置。 具体而言，提供了允许核酸扩增的修饰聚合酶及其生物活性片段。 在一个方面，本公开涉及用于核酸测序，基因分型，拷贝数变异分析，配对末端测序和其他形式的遗传分析的修饰的聚合酶。 在一些方面，本公开涉及用于产生供各种下游过程使用的核酸文库或核酸模板的修饰聚合酶。 在一些方面，本公开涉及鉴定可以跨聚合酶的类或家族转移以提供具有改变的催化性质的新聚合酶的同源氨基酸突变。 在一些方面，本公开内容提供了与参考聚合酶相比具有增强的催化性质的修饰聚合酶。

    LIBRARY PREPARATION METHODS AND COMPOSITIONS AND USES THEREFOR
    3.
    发明申请
    LIBRARY PREPARATION METHODS AND COMPOSITIONS AND USES THEREFOR 审中-公开

    公开(公告)号：WO2019006392A1

    公开(公告)日：2019-01-03

    申请号：PCT/US2018/040432

    申请日：2018-06-29

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： ANDERSEN, Mark ,  MAZUR, Daniel

    IPC: C12Q1/6806 ,  C12Q1/6853

    Abstract: Provided are methods for preparing a library of target nucleic acid sequences, as well as compositions and uses therefor. Methods comprise contacting a nucleic acid sample with a plurality of adaptors capable of amplification of one or more target nucleic acid sequences under conditions wherein the target nucleic acid(s) undergo a first amplification; digesting the resulting first amplification products; repairing the digested target amplicons; and amplifying the repaired products in a second amplification, thereby producing a library of target nucleic acid sequence. Each of the plurality of adaptor compositions comprise a handle and a targeted nucleic acid sequence and optionally one or more tag sequences. Provided methods may be carried out in a single, addition only workflow reaction, allowing for rapid production of highly multiplexed targeted libraries, optionally including unique tag sequences. Resulting library compositions are useful for a variety of applications, including sequencing applications.

    POLYMERASE COMPOSITIONS AND METHODS OF MAKING AND USING SAME
    4.
    发明申请
    POLYMERASE COMPOSITIONS AND METHODS OF MAKING AND USING SAME 审中-公开
    聚合酶组合物及其制备和使用方法

    公开(公告)号：WO2016100438A2

    公开(公告)日：2016-06-23

    申请号：PCT/US2015/065977

    申请日：2015-12-16

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： MAZUR, Daniel ,  TOZER, Eileen ,  CHEN, Sihong ,  VANDER HORN, Peter ,  LINCECUM, Tommie

    IPC: C12N9/12 ,  C12Q1/68

    CPC classification number: C12Q1/686 ,  C12N9/1252 ,  C12Q1/6869 ,  C12Q2521/101 ,  C12Y207/07007

    Abstract: The present disclosure provides compositions, methods, kits, systems and apparatus that are useful for nucleic acid polymerization. In particular, modified polymerases and biologically active fragments thereof, such as modified Taq polymerases, are provided that allow for improved nucleic acid amplification. In some aspects, the disclosure provides modified polymerases having improved thermostability, accuracy, processivity and/or read length as compared to a referenceTaq polymerase. In some aspects, the disclosure relates to modified polymerases or biologically active fragments thereof, useful for amplification methods, and in practically illustrative embodiments, emulsion PCR.

    Abstract translation: 本公开提供了用于核酸聚合的组合物，方法，试剂盒，系统和装置。 具体而言，提供了修饰的聚合酶及其生物活性片段，例如修饰的Taq聚合酶，其允许改善的核酸扩增。 在一些方面，本公开内容提供了与参照Taq聚合酶相比具有改善的热稳定性，准确性，持续合成能力和/或阅读长度的修饰的聚合酶。 在一些方面，本公开涉及可用于扩增方法的修饰聚合酶或其生物活性片段，并且在实际示例性实施方案中为乳液PCR。

    POLYMERASE COMPOSITIONS, METHODS OF MAKING AND USING SAME
    6.
    发明申请
    POLYMERASE COMPOSITIONS, METHODS OF MAKING AND USING SAME 审中-公开
    聚合物组合物，制备方法和使用方法

    公开(公告)号：WO2015048763A1

    公开(公告)日：2015-04-02

    申请号：PCT/US2014/058361

    申请日：2014-09-30

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： VANDER HORN, Peter ,  MAZUR, Daniel ,  NIKIFOROV, Theo ,  LANDES, Mindy ,  TOZER, Eileen

    IPC: C12Q1/68 ,  C12N9/12

    CPC classification number: C12N9/1252 ,  C12Q1/686 ,  C12Q1/6869

    Abstract: The present disclosure provides compositions, methods, kits, systems and apparatus that are useful for nucleic acid polymerization. In particular, modified polymerases and biologically active fragments thereof are provided that allow for nucleic acid amplification. In some aspects, the disclosure provides modified polymerases having lower systematic error as compared to a reference polymerase. In one aspect, the disclosure relates to modified polymerases useful for nucleic acid sequencing, genotyping, copy number variation analysis, paired-end sequencing and other forms of genetic analysis. In some aspects, the disclosure relates to modified polymerases useful for the generation of nucleic acid libraries or nucleic acid templates. In some aspects, the disclosure relates to the identification of homologous amino acid mutations that can be transferred across classes or families of polymerases to provide novel polymerases with altered properties.

    Abstract translation: 本公开提供了可用于核酸聚合的组合物，方法，试剂盒，系统和装置。 特别地，提供了允许核酸扩增的修饰的聚合酶及其生物活性片段。 在一些方面，本公开提供与参考聚合酶相比具有较低系统误差的修饰聚合酶。 一方面，本公开涉及可用于核酸测序，基因分型，拷贝数变异分析，配对末端测序和其他形式遗传分析的修饰聚合酶。 在一些方面，本公开涉及可用于产生核酸文库或核酸模板的修饰聚合酶。 在一些方面，本公开涉及可以跨越聚合酶类或家族转移的同源氨基酸突变的鉴定，以提供具有改变的性质的新型聚合酶。

    LIBRARY PREPARATION METHODS AND COMPOSITIONS AND USES THEREFOR
    7.
    发明公开
    LIBRARY PREPARATION METHODS AND COMPOSITIONS AND USES THEREFOR 审中-公开

    公开(公告)号：EP4282982A3

    公开(公告)日：2024-01-10

    申请号：EP23192384.8

    申请日：2018-06-29

    Applicant: Life Technologies Corporation

    Inventor： ANDERSEN, Mark ,  MAZUR, Daniel ,  CHEN, Sihong ,  LUO, Guobin ,  PENG, Xinzhan

    IPC: C12Q1/6806 ,  C12Q1/6853

    Abstract: Provided are methods for preparing a library of target nucleic acid sequences, as well as compositions and uses therefor. Methods comprise contacting a nucleic acid sample with a plurality of adaptors capable of amplification of one or more target nucleic acid sequences under conditions wherein the target nucleic acid(s) undergo a first amplification; digesting the resulting first amplification products; repairing the digested target amplicons; and amplifying the repaired products in a second amplification, thereby producing a library of target nucleic acid sequence. Each of the plurality of adaptor compositions comprise a handle and a targeted nucleic acid sequence and optionally one or more tag sequences. Provided methods may be carried out in a single, addition only workflow reaction, allowing for rapid production of highly multiplexed targeted libraries, optionally including unique tag sequences. Resulting library compositions are useful for a variety of applications, including sequencing applications.

    LIBRARY PREPARATION METHODS AND COMPOSITIONS AND USES THEREFOR
    8.
    发明公开
    LIBRARY PREPARATION METHODS AND COMPOSITIONS AND USES THEREFOR 审中-公开

    公开(公告)号：EP3957746A1

    公开(公告)日：2022-02-23

    申请号：EP21191446.0

    申请日：2018-06-29

    Applicant: Life Technologies Corporation

    Inventor： ANDERSEN, Mark ,  MAZUR, Daniel ,  CHEN, Sihong ,  LUO, Guobin ,  PENG, Xinzhan

    IPC: C12Q1/6806 ,  C12Q1/6853

    Abstract: Provided are methods for preparing a library of target nucleic acid sequences, as well as compositions and uses therefor. Methods comprise contacting a nucleic acid sample with a plurality of adaptors capable of amplification of one or more target nucleic acid sequences under conditions wherein the target nucleic acid(s) undergo a first amplification; digesting the resulting first amplification products; repairing the digested target amplicons; and amplifying the repaired products in a second amplification, thereby producing a library of target nucleic acid sequence. Each of the plurality of adaptor compositions comprise a handle and a targeted nucleic acid sequence and optionally one or more tag sequences. Provided methods may be carried out in a single, addition only workflow reaction, allowing for rapid production of highly multiplexed targeted libraries, optionally including unique tag sequences. Resulting library compositions are useful for a variety of applications, including sequencing applications.

    POLYMERASE COMPOSITIONS
    10.
    发明授权
    POLYMERASE COMPOSITIONS 有权
    聚合酶组合物

    公开(公告)号：EP2742151B1

    公开(公告)日：2017-10-25

    申请号：EP12751661.5

    申请日：2012-08-10

    Applicant: Life Technologies Corporation

    Inventor： VANDER HORN, Peter ,  NIKIFOROV, Theo ,  LUO, Guobin ,  LANDES, Mindy ,  MAZUR, Daniel ,  TOZER, Eileen ,  LINCECUM, Tommie

    IPC: C12Q1/68 ,  C12N9/12

    CPC classification number: C12N9/1252 ,  C12Q1/6846 ,  C12Q1/686 ,  C12Y207/07007

    Abstract: The present disclosure provides compositions, methods, kits, systems and apparatus that are useful for nucleic acid polymerization. In particular, modified polymerases and biologically active fragment thereof are provided that allow for nucleic acid amplification. In one aspect, the disclosure relates to modified polymerases useful for nucleic acid sequencing, genotyping, copy number variation analysis, paired-end sequencing and other forms of genetic analysis. In some aspects, the disclosure relates to modified polymerases useful for the generation of nucleic acid libraries or nucleic acid templates for use in various downstream processes. In some aspects, the disclosure relates to the identification of homologous amino acid mutations that can be transferred across classes or families of polymerases to provide novel polymerases with altered catalytic properties. In some aspects, the disclosure provides modified polymerases having enhanced catalytic properties as compared to a reference polymerase.

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