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1.
公开(公告)号:WO2016090076A2
公开(公告)日:2016-06-09
申请号:PCT/US2015/063593
申请日:2015-12-03
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor: ZHOU, Wenjun , GEE, Kyle , TENNICO, Yolanda , SLADE, Peter , KANG, Hee Chol , KHAN, Shaheer , EVANS, Brian , STRAY, James
IPC: C07D261/00 , G01N33/58
CPC classification number: C07C309/47 , C07C309/51 , C07C2603/50 , C07D311/84 , G01N33/582 , G01N2400/10
Abstract: The present disclosure is directed dye compounds containing a hydrazinyl substituent and optionally, one or more negatively charged groups, such as sulfonate, phosphate, phosphonate, and/or carboxylate groups and dye compounds containing an aminooxy substitutent. The compounds are useful in the detection of analytes containing aldehyde and ketone groups, including, for example, glycans.
Abstract translation: 本公开涉及含有肼基取代基和任选的一个或多个带负电荷的基团的染料化合物,例如磺酸盐,磷酸盐,膦酸盐和/或羧酸盐基团以及含有氨基氧基取代基的染料化合物。 该化合物可用于检测含醛和酮基的分析物,包括例如聚糖。
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公开(公告)号:WO2016090165A1
公开(公告)日:2016-06-09
申请号:PCT/US2015/063791
申请日:2015-12-03
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor: STRAY, James , KHAN, Shaheer
IPC: G06F19/00 , C12P19/00 , G01N33/66 , G01N33/58 , G01N21/00 , G01N33/533 , G01N27/447 , G01N33/53 , G01N30/00 , G01N30/88
CPC classification number: G01N33/66 , C12P19/14 , G01N21/6428 , G01N21/76 , G01N27/447 , G01N27/44726 , G01N30/00 , G01N33/5308 , G01N33/533 , G01N33/58 , G01N2021/6441 , G01N2030/8836 , G01N2333/924 , G01N2400/00 , G01N2458/10 , G06F19/70
Abstract: Methods, systems, compositions and kits are described for detecting cleaved glycans from a glycoconjugate. After labeling the glycan with a nucleic acid charged oligomer described herein, the labeled glycans can be separated under the influence of an electric field or based on their detectable tag and identified.
Abstract translation: 描述了用于从糖缀合物检测切割的聚糖的方法,系统,组合物和试剂盒。 在用本文所述的带有核酸的寡核苷酸标记聚糖之后,标记的聚糖可以在电场的影响下或基于其可检测的标签分离并鉴定。
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公开(公告)号:WO2011041703A2
公开(公告)日:2011-04-07
申请号:PCT/US2010/051165
申请日:2010-10-01
Applicant: LIFE TECHNOLOGIES CORPORATION , BREVNOV, Maxim G. , NURSE, James C. , STRAY, James
Inventor: BREVNOV, Maxim G. , NURSE, James C. , STRAY, James
CPC classification number: G01N1/4005 , G01N2001/4016
Abstract: A device for sample processing can include at least one chamber having an egress, said chamber being configured to receive a sample for processing, a filter through which at least some sample portions in the at least one chamber flow, and a barrier member disposed in a first state to contain sample in the at least one chamber. Upon sufficient conditions, the barrier member can be alterable to a second state to permit flow of at least some sample portions contained in the chamber in a flow direction toward the egress and through the filter.
Abstract translation: 用于样品处理的装置可以包括具有出口的至少一个室,所述室被配置为接收用于处理的样品,过滤器,所述至少一个室中的至少一些样品部分通过所述过滤器和设置在所述室中的阻挡构件 第一状态以在至少一个室中容纳样品。 在足够的条件下,阻挡构件可以改变到第二状态,以允许包含在腔室中的至少一些样品部分沿流动方向流向出口并通过过滤器。
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公开(公告)号:WO2011034864A1
公开(公告)日:2011-03-24
申请号:PCT/US2010/048833
申请日:2010-09-14
Applicant: LIFE TECHNOLOGIES CORPORATION , STRAY, James , LIU, Yingjie (Jason)
Inventor: STRAY, James , LIU, Yingjie (Jason)
CPC classification number: C12N15/1003 , C12N1/06 , C12N15/1006 , C12N15/1013 , C12Q1/6806
Abstract: The present teachings relate to the extraction of nucleic acid from solid materials. Provided are useful compositions, methods and kits for obtaining nucleic acids from a solid biological sample or an adhesive material having a biological material adherent or embedded within the adhesive substrate. The extracted nucleic acid can be used in downstream applications such as genotyping, detection, quantification, and identification of the source of the biological material.
Abstract translation: 本教导涉及从固体材料中提取核酸。 提供了用于从固体生物样品获得核酸的有用的组合物,方法和试剂盒或具有粘附或嵌入粘合剂基底内的生物材料的粘合剂材料。 提取的核酸可用于下游应用,例如生物材料来源的基因分型,检测,定量和鉴定。
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5.
公开(公告)号:EP3227264A2
公开(公告)日:2017-10-11
申请号:EP15828557.7
申请日:2015-12-03
Applicant: Life Technologies Corporation
Inventor: ZHOU, Wenjun , GEE, Kyle , TENNICO, Yolanda , SLADE, Peter , KANG, Hee Chol , KHAN, Shaheer , EVANS, Brian , STRAY, James
IPC: C07D261/00 , G01N33/58
Abstract: The present disclosure is directed dye compounds containing a hydrazinyl substituent and optionally, one or more negatively charged groups, such as sulfonate, phosphate, phosphonate, and/or carboxylate groups and dye compounds containing an aminooxy substitutent. The compounds are useful in the detection of analytes containing aldehyde and ketone groups, including, for example, glycans.
Abstract translation: 本公开内容涉及含有肼基取代基和任选地一个或多个带负电基团(例如磺酸根,磷酸根,膦酸根和/或羧酸根基团)和含有氨氧基取代基的染料化合物的染料化合物。 这些化合物可用于检测含有醛和酮基的分析物,包括例如聚糖。
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公开(公告)号:EP3227264B1
公开(公告)日:2020-10-21
申请号:EP15828557.7
申请日:2015-12-03
Applicant: Life Technologies Corporation
Inventor: ZHOU, Wenjun , GEE, Kyle , TENNICO, Yolanda , SLADE, Peter , KANG, Hee Chol , KHAN, Shaheer , EVANS, Brian , STRAY, James
IPC: C07D261/00 , G01N33/58
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公开(公告)号:EP2478088B1
公开(公告)日:2016-08-31
申请号:EP10755294.5
申请日:2010-09-14
Applicant: Life Technologies Corporation
Inventor: STRAY, James , LIU, Yingjie (Jason)
CPC classification number: C12N15/1003 , C12N1/06 , C12N15/1006 , C12N15/1013 , C12Q1/6806
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公开(公告)号:EP2483657A2
公开(公告)日:2012-08-08
申请号:EP10821349.7
申请日:2010-10-01
Applicant: Life Technologies Corporation
Inventor: BREVNOV, Maxim G. , NURSE, James C. , STRAY, James
CPC classification number: G01N1/4005 , G01N2001/4016
Abstract: A device for sample processing can include at least one chamber having an egress, said chamber being configured to receive a sample for processing, a filter through which at least some sample portions in the at least one chamber flow, and a barrier member disposed in a first state to contain sample in the at least one chamber. Upon sufficient conditions, the barrier member can be alterable to a second state to permit flow of at least some sample portions contained in the chamber in a flow direction toward the egress and through the filter.
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公开(公告)号:EP2478088A1
公开(公告)日:2012-07-25
申请号:EP10755294.5
申请日:2010-09-14
Applicant: Life Technologies Corporation
Inventor: STRAY, James , LIU, Yingjie (Jason)
CPC classification number: C12N15/1003 , C12N1/06 , C12N15/1006 , C12N15/1013 , C12Q1/6806
Abstract: The present teachings relate to the extraction of nucleic acid from solid materials. Provided are useful compositions, methods and kits for obtaining nucleic acids from a solid biological sample or an adhesive material having a biological material adherent or embedded within the adhesive substrate. The extracted nucleic acid can be used in downstream applications such as genotyping, detection, quantification, and identification of the source of the biological material.
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