公开(公告)号：WO2020037284A1

公开(公告)日：2020-02-20

申请号：PCT/US2019/046941

申请日：2019-08-16

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： YU, Hua ,  ZHENG, Rui ,  YANG, Xiaoling ,  WONG, Chiu Tai Andrew ,  GRAY, Jeremy

IPC: C12Q1/6806 ,  C12Q1/6874 ,  B01L3/00 ,  C12Q1/6844 ,  C40B50/14 ,  G01N35/00

Abstract: The disclosure generally relates to systems, methods, and apparatuses for magnetic bead loading. An example embodiment of the disclosure relates to mixing magnetic beads with sequencing beads to form a solution. The solution containing both beads is injected onto a microchip having a plurality of microwells. The magnetic beads may have larger diameter than the microwell while the sequencing beads may have a smaller diameter, allowing them to enter and reside in the microwell. One or more magnets positioned under the microchip move back and forth across the microchip surface. The magnetic beads form a line and follow the movement of the magnets. During rounds of sweeping, the sequencing beads load into the respective wells. The magnets may be disengaged and the magnetic beads may be washed away after the sequencing beads are loaded.

公开(公告)号：WO2015070103A1

公开(公告)日：2015-05-14

申请号：PCT/US2014/064691

申请日：2014-11-07

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： BARBEE, Kristohper ,  ZHENG, Rui ,  MCCUSKER, Sean

IPC: B04B5/04 ,  B04B11/04

CPC classification number: B04B7/08 ,  B01D21/262 ,  B04B5/0421 ,  B04B2011/046 ,  G01N2035/00158 ,  G01N2035/00495 ,  G01N2035/0449

Abstract: A rotor assembly includes a rotor plate (102) to rotate around a first axis (116); a bucket (104) rotatably attached to the rotor plate and to rotate around a second axis; and a stop plate (110) to rotate around the first axis (116) relative to the rotor plate between an open position and a closed position, when in the closed position, the stop plate (110) is to engage the bucket (104) to fix an angular position of the bucket relative to a plane of rotation of the rotor assembly.

Abstract translation: 转子组件包括围绕第一轴线（116）旋转的转子板（102）; 可旋转地附接到所述转子板并围绕第二轴线旋转的铲斗（104） 以及在打开位置和关闭位置之间相对于转子板绕第一轴线（116）旋转的止动板（110），当处于关闭位置时，止动板（110）将与铲斗（104）接合， 以相对于转子组件的旋转平面固定铲斗的角位置。

公开(公告)号：EP3068541B1

公开(公告)日：2020-10-28

申请号：EP14812319.3

申请日：2014-11-07

Applicant: Life Technologies Corporation

Inventor： BARBEE, Kristopher ,  ZHENG, Rui ,  MCCUSKER, Sean

IPC: B04B5/04 ,  B04B11/04 ,  G01N35/00 ,  G01N35/04

公开(公告)号：EP3068541A1

公开(公告)日：2016-09-21

申请号：EP14812319.3

申请日：2014-11-07

Applicant: Life Technologies Corporation

Inventor： BARBEE, Kristohper ,  ZHENG, Rui ,  MCCUSKER, Sean

IPC: B04B5/04 ,  B04B11/04

Abstract: A rotor assembly includes a rotor plate to rotate around a first axis; a bucket rotatably attached to the rotor plate and to rotate around a second axis; and a stop plate to rotate around the first axis relative to the rotor plate between an open position and a closed position, when in the closed position, the stop plate is to engage the bucket to fix an angular position of the bucket relative to a plane of rotation of the rotor assembly.

Abstract translation: 转子组件包括围绕第一轴线（116）旋转的转子板（102）; 可旋转地连接到所述转子板并围绕第二轴线旋转的铲斗（104） 和止动板（110），所述止动板（110）在打开位置和关闭位置之间围绕所述第一轴线（116）相对于所述转子板旋转，当处于所述关闭位置时，所述止动板（110）将接合所述铲斗（104） 以固定铲斗相对于转子组件的旋转平面的角位置。