公开(公告)号：WO2013165594A1

公开(公告)日：2013-11-07

申请号：PCT/US2013/031640

申请日：2013-03-14

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： BARBEE, Kristopher ,  JONES, Ryan ,  MCCUSKER, Sean ,  CARPINO, Maximilian ,  LEAMON, John ,  SCHULTZ, Jonathan ,  HOSHIZAKI, Jon

IPC: B01L7/00 ,  C12Q1/68 ,  G01N35/10 ,  G01N35/00

CPC classification number: C12Q1/6806 ,  B04B5/0421 ,  B04B13/00 ,  C12Q1/6869 ,  G01N35/00693 ,  G01N35/1011 ,  G01N2035/00495 ,  G01N2035/1013 ,  G01N2035/103

Abstract: An apparatus includes a robotic system providing movement in three orthogonal directions to an arm operable to receive a pipette tip and to facilitate movement of fluid into and out of the pipette tip. In addition, the apparatus can include a tray for receiving pipette tips, receptacles for receiving tubes, an apparatus for forming an emulsion, a centrifuge for breaking the emulsion, a device for forming particles that include copies of the polynucleotide, a device for enriching the particles and an apparatus for loading such particles onto a sensor array. The apparatus can further include receptacles for holding containers of reagent solutions. Optionally, the robot can include a gripper arm in addition to the pipette receiving arm. A method for calibrating the z-position of a translation device moving a pipette Hp is included. An imaging system is used for assessing attachement of pipette tip.

Abstract translation: 一种装置包括机器人系统，该机器人系统在三个正交方向上提供可操作以接收移液管尖端的臂并促进流体进入和移出移液管尖端的运动。 此外，该装置可以包括用于接收移液管吸头的托盘，用于接收管的容器，用于形成乳液的装置，用于破乳的离心机，用于形成包括多核苷酸的拷贝的颗粒的装置，用于富集 颗粒和用于将这种颗粒装载到传感器阵列上的装置。 该装置还可以包括容纳试剂溶液容器的容器。 可选地，除了移液管接收臂之外，机器人还可以包括夹持臂。 包括用于校准移动移液管Hp的翻译装置的z位置的方法。 成像系统用于评估移液管尖端的附着。

公开(公告)号：WO2015070103A1

公开(公告)日：2015-05-14

申请号：PCT/US2014/064691

申请日：2014-11-07

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： BARBEE, Kristohper ,  ZHENG, Rui ,  MCCUSKER, Sean

IPC: B04B5/04 ,  B04B11/04

CPC classification number: B04B7/08 ,  B01D21/262 ,  B04B5/0421 ,  B04B2011/046 ,  G01N2035/00158 ,  G01N2035/00495 ,  G01N2035/0449

Abstract: A rotor assembly includes a rotor plate (102) to rotate around a first axis (116); a bucket (104) rotatably attached to the rotor plate and to rotate around a second axis; and a stop plate (110) to rotate around the first axis (116) relative to the rotor plate between an open position and a closed position, when in the closed position, the stop plate (110) is to engage the bucket (104) to fix an angular position of the bucket relative to a plane of rotation of the rotor assembly.

Abstract translation: 转子组件包括围绕第一轴线（116）旋转的转子板（102）; 可旋转地附接到所述转子板并围绕第二轴线旋转的铲斗（104） 以及在打开位置和关闭位置之间相对于转子板绕第一轴线（116）旋转的止动板（110），当处于关闭位置时，止动板（110）将与铲斗（104）接合， 以相对于转子组件的旋转平面固定铲斗的角位置。

公开(公告)号：EP2844392A1

公开(公告)日：2015-03-11

申请号：EP13713643.8

申请日：2013-03-14

Applicant: Life Technologies Corporation

Inventor： BARBEE, Kristopher ,  JONES, Ryan ,  MCCUSKER, Sean ,  CARPINO, Maximilian ,  LEAMON, John ,  SCHULTZ, Jonathan ,  HOSHIZAKI, Jon

IPC: B01L7/00 ,  C12Q1/68 ,  G01N35/10 ,  G01N35/00

CPC classification number: C12Q1/6806 ,  B04B5/0421 ,  B04B13/00 ,  C12Q1/6869 ,  G01N35/00693 ,  G01N35/1011 ,  G01N2035/00495 ,  G01N2035/1013 ,  G01N2035/103

Abstract: An apparatus includes a robotic system providing movement in three orthogonal directions to an arm operable to receive a pipette tip and to facilitate movement of fluid into and out of the pipette tip. In addition, the apparatus can include a tray for receiving pipette tips, receptacles for receiving tubes, an apparatus for forming an emulsion, a centrifuge for breaking the emulsion, a device for forming particles that include copies of the polynucleotide, a device for enriching the particles and an apparatus for loading such particles onto a sensor array. The apparatus can further include receptacles for holding containers of reagent solutions. Optionally, the robot can include a gripper arm in addition to the pipette receiving arm. A method for calibrating the z-position of a translation device moving a pipette Hp is included. An imaging system is used for assessing attachement of pipette tip.

公开(公告)号：EP3068541B1

公开(公告)日：2020-10-28

申请号：EP14812319.3

申请日：2014-11-07

Applicant: Life Technologies Corporation

Inventor： BARBEE, Kristopher ,  ZHENG, Rui ,  MCCUSKER, Sean

IPC: B04B5/04 ,  B04B11/04 ,  G01N35/00 ,  G01N35/04

公开(公告)号：EP3068541A1

公开(公告)日：2016-09-21

申请号：EP14812319.3

申请日：2014-11-07

Applicant: Life Technologies Corporation

Inventor： BARBEE, Kristohper ,  ZHENG, Rui ,  MCCUSKER, Sean

IPC: B04B5/04 ,  B04B11/04

Abstract: A rotor assembly includes a rotor plate to rotate around a first axis; a bucket rotatably attached to the rotor plate and to rotate around a second axis; and a stop plate to rotate around the first axis relative to the rotor plate between an open position and a closed position, when in the closed position, the stop plate is to engage the bucket to fix an angular position of the bucket relative to a plane of rotation of the rotor assembly.

Abstract translation: 转子组件包括围绕第一轴线（116）旋转的转子板（102）; 可旋转地连接到所述转子板并围绕第二轴线旋转的铲斗（104） 和止动板（110），所述止动板（110）在打开位置和关闭位置之间围绕所述第一轴线（116）相对于所述转子板旋转，当处于所述关闭位置时，所述止动板（110）将接合所述铲斗（104） 以固定铲斗相对于转子组件的旋转平面的角位置。