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Patent Agency Ranking
公开(公告)号：WO03062418B1
公开(公告)日：2004-04-01
申请号：PCT/JP0300668
申请日：2003-01-24
Applicant: OLYMPUS CORP , KOIKE HISASHI , NAGAOKA TOMONORI , SATOH TAKATOMO , KANEKO YOSHIOKI , HATANAKA MIDORI , FUKUOKA MORINAO , SAKAMOTO HIROKO , YONEKAWA HIROYUKI
Inventor： KOIKE HISASHI , NAGAOKA TOMONORI , SATOH TAKATOMO , KANEKO YOSHIOKI , HATANAKA MIDORI , FUKUOKA MORINAO , SAKAMOTO HIROKO , YONEKAWA HIROYUKI
CPC classification number: C12Q1/6827 , C12Q1/6837 , C12Q2565/501 , C12Q2561/12 , C12Q2527/107
Abstract: It is intended to provide a method of detecting the data of a target nucleic acid comprising bringing the target nucleic acid into contact with a probe having a sequence being complementary to at leas a part of the target nucleic acid sequence to form a hybrid of the target nucleic acid with the probe, and measuring a signal generated in an amount depending on the amount of the hybrid to thereby detect the data of the target nucleic acid, wherein the signal data are kinetically obtained. It is also intended to provide a method of detecting the data of a target nucleic acid comprising bringing the target nucleic acid into contact with a completely matching probe being completely complementary to at leas a part of the target nucleic acid sequence and one or more incompletely matching probes having a variation in at least a part of the completely matching probe to form hybrids of the target nucleic acid with the completely matching probe and with the incompletely matching probe, and detecting the data of the target nucleic acid based on the difference in the binding strength between the hybrids, characterized in that the signal data are kinetically obtained while continuously or stepwise altering the conditions of measuring or detecting the signals generated from the hybrids.
Abstract translation: 旨在提供一种检测目标核酸的数据的方法，包括使靶核酸与具有与靶核酸序列的一部分互补的序列的探针接触，以形成目标核酸的杂交体 核酸，并且测量根据混合物的量产生的量的信号，从而检测目标核酸的数据，其中信号数据被动力学地获得。 还旨在提供一种检测靶核酸的数据的方法，包括使靶核酸与完全匹配的探针完全互补，所述完全匹配的探针与目标核酸序列的一部分完全互补，并且一个或多个不完全匹配 探针在完全匹配的探针的至少一部分上具有变异，以与完全匹配的探针和不完全匹配的探针形成靶核酸的杂交体，并且基于结合的差异检测靶核酸的数据 其特征在于，在连续或逐步地改变测量或检测从杂种产生的信号的条件下动态获得信号数据。
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公开(公告)号：EP1469067A4
公开(公告)日：2007-05-23
申请号：EP03703050
申请日：2003-01-24
Applicant: OLYMPUS CORP
Inventor： KOIKE HISASHI , NAGAOKA TOMONORI , SATOH TAKATOMO , KANEKO YOSHIOKI , HATANAKA MIDORI , FUKUOKA MORINAO , SAKAMOTO HIROKO , YONEKAWA HIROYUKI
CPC classification number: C12Q1/6827 , C12Q1/6837 , C12Q2565/501 , C12Q2561/12 , C12Q2527/107
Abstract: It is intended to provide a method of detecting the data of a target nucleic acid comprising bringing the target nucleic acid into contact with a probe having a sequence being complementary to at leas a part of the target nucleic acid sequence to form a hybrid of the target nucleic acid with the probe, and measuring a signal generated in an amount depending on the amount of the hybrid to thereby detect the data of the target nucleic acid, wherein the signal data are kinetically obtained. It is also intended to provide a method of detecting the data of a target nucleic acid comprising bringing the target nucleic acid into contact with a completely matching probe being completely complementary to at leas a part of the target nucleic acid sequence and one or more incompletely matching probes having a variation in at least a part of the completely matching probe to form hybrids of the target nucleic acid with the completely matching probe and with the incompletely matching probe, and detecting the data of the target nucleic acid based on the difference in the binding strength between the hybrids, characterized in that the signal data are kinetically obtained while continuously or stepwise altering the conditions of measuring or detecting the signals generated from the hybrids.
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公开(公告)号：JP2004093165A
公开(公告)日：2004-03-25
申请号：JP2002250989
申请日：2002-08-29
Applicant: Olympus Corp , オリンパス株式会社
Inventor： KANEKO YOSHIOKI , SATO TAKATOMO , SAKAMOTO MICHIKO , HATANAKA MIDORI , NAGAOKA TOMONORI , FUKUOKA TAKAHISA
Abstract: PROBLEM TO BE SOLVED: To provide a hybridization detection method for avoiding nonspecific hybrid formation, without causing the hybridization strength of a probe to decrease and detecting a signal by a specific reaction, with high sensitivity.
SOLUTION: In the hybridization detection method, a labeled target is brought into contact with a microarray where a plurality of different probes are arranged, hybrids are formed, the signal of a hybridized, labeled target molecule is measured for identifying the hybridized target molecule, thus dissociating intramolecular combination of a probe and/or intermolecular combination, before bringing the labeled target into contact with the microarray.
COPYRIGHT: (C)2004,JPO-
公开(公告)号：JP2004028620A
公开(公告)日：2004-01-29
申请号：JP2002181460
申请日：2002-06-21
Applicant: Olympus Corp , オリンパス株式会社
Inventor： HATANAKA MIDORI , SATO TAKATOMO , SAKAMOTO MICHIKO
Abstract: PROBLEM TO BE SOLVED: To provide a quick, accurate point gene mutation analysis method.
SOLUTION: In the gene mutation analysis method: a complete match probe and an incomplete one are brought into contact with a target nucleic acid for each kind of probe, a hybrid is formed between them, and a signal that changes based on a difference in the bond strength of the hybrid and is generated from the hybrid is detected, thus analyzing sequence information on the target mucleic acid. In this case, the target mucleic acid is prepared by PCR, temperature for forming the hybrid differs from that for detecting the signal, and a liquid sample containing the target mucleic acid flows inside and outside a DNA microarray where a plurality of minute liquid accommodation sections capable of accommodating the liquid three-dimensionally are arranged two-dimensionally at each temperature.
COPYRIGHT: (C)2004,JPO-
公开(公告)号：JP2004135663A
公开(公告)日：2004-05-13
申请号：JP2003330287
申请日：2003-09-22
Applicant: Olympus Corp , オリンパス株式会社
Inventor： SATO TAKATOMO , HATANAKA MIDORI , SAKAMOTO MICHIKO , KANEKO YOSHIOKI , NAGAOKA TOMONORI
Abstract: PROBLEM TO BE SOLVED: To provide a method for analyzing gene expression, capable of analyzing the gene expression at a high rate with good accuracy, by using a three-dimensional micro-array.
SOLUTION: This method for analyzing the gene expression comprises analyzing a state of the gene expression by using the micro-array in which two or more minute liquid-receiving parts capable of three-dimensionally receiving the liquid are two-dimensionally arranged. Further, the method includes a process for preparing a labeled nucleic acid by using a RNA as a template, another process for positioning a DNA probe which complementarily combines with a part of the labeled gene on the liquid-receiving part, a third process for contacting a liquid sample which contains the labeled nucleic acid with the DNA probe under a condition that a hybrid is formed between the labeled nucleic acid and the DNA probe by making the liquid sample flow inside and outside the micro-array, and the other process for detecting signal strength which changes based on difference of combining strength of the hybrid under another condition that formation of a nonspecific hybrid is dissociated.
COPYRIGHT: (C)2004,JPOAbstract translation: 待解决的问题：提供一种分析基因表达的方法，其能够通过使用三维微阵列以高精度高分析基因表达。 解决方案：用于分析基因表达的方法包括通过使用其中能够三维接收液体的两个或更多个微小液体接收部分二维排列的微阵列来分析基因表达的状态。 此外，该方法包括通过使用RNA作为模板来制备标记的核酸的方法，用于定位与液体接收部分上的部分标记基因互补结合的DNA探针的另一种方法，第三种接触方法 在通过使液体样品在微阵列内外流动而在标记的核酸和DNA探针之间形成杂交条件的条件下，将DNA标记的核酸与DNA探针混合的液体试样， 基于在非特异性杂交体的形成解离的另一条件下，杂交体的组合强度的差异而发生变化的信号强度。 版权所有（C）2004，JPO
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