公开(公告)号：WO03062418B1

公开(公告)日：2004-04-01

申请号：PCT/JP0300668

申请日：2003-01-24

Applicant: OLYMPUS CORP ,  KOIKE HISASHI ,  NAGAOKA TOMONORI ,  SATOH TAKATOMO ,  KANEKO YOSHIOKI ,  HATANAKA MIDORI ,  FUKUOKA MORINAO ,  SAKAMOTO HIROKO ,  YONEKAWA HIROYUKI

Inventor： KOIKE HISASHI ,  NAGAOKA TOMONORI ,  SATOH TAKATOMO ,  KANEKO YOSHIOKI ,  HATANAKA MIDORI ,  FUKUOKA MORINAO ,  SAKAMOTO HIROKO ,  YONEKAWA HIROYUKI

IPC: C12N15/09 ,  C12Q1/68 ,  G01N33/50 ,  G01N33/53 ,  G01N33/566 ,  G01N33/58

CPC classification number: C12Q1/6827 ,  C12Q1/6837 ,  C12Q2565/501 ,  C12Q2561/12 ,  C12Q2527/107

Abstract: It is intended to provide a method of detecting the data of a target nucleic acid comprising bringing the target nucleic acid into contact with a probe having a sequence being complementary to at leas a part of the target nucleic acid sequence to form a hybrid of the target nucleic acid with the probe, and measuring a signal generated in an amount depending on the amount of the hybrid to thereby detect the data of the target nucleic acid, wherein the signal data are kinetically obtained. It is also intended to provide a method of detecting the data of a target nucleic acid comprising bringing the target nucleic acid into contact with a completely matching probe being completely complementary to at leas a part of the target nucleic acid sequence and one or more incompletely matching probes having a variation in at least a part of the completely matching probe to form hybrids of the target nucleic acid with the completely matching probe and with the incompletely matching probe, and detecting the data of the target nucleic acid based on the difference in the binding strength between the hybrids, characterized in that the signal data are kinetically obtained while continuously or stepwise altering the conditions of measuring or detecting the signals generated from the hybrids.

Abstract translation: 旨在提供一种检测目标核酸的数据的方法，包括使靶核酸与具有与靶核酸序列的一部分互补的序列的探针接触，以形成目标核酸的杂交体 核酸，并且测量根据混合物的量产生的量的信号，从而检测目标核酸的数据，其中信号数据被动力学地获得。 还旨在提供一种检测靶核酸的数据的方法，包括使靶核酸与完全匹配的探针完全互补，所述完全匹配的探针与目标核酸序列的一部分完全互补，并且一个或多个不完全匹配 探针在完全匹配的探针的至少一部分上具有变异，以与完全匹配的探针和不完全匹配的探针形成靶核酸的杂交体，并且基于结合的差异检测靶核酸的数据 其特征在于，在连续或逐步地改变测量或检测从杂种产生的信号的条件下动态获得信号数据。

公开(公告)号：EP1469067A4

公开(公告)日：2007-05-23

申请号：EP03703050

申请日：2003-01-24

Applicant: OLYMPUS CORP

Inventor： KOIKE HISASHI ,  NAGAOKA TOMONORI ,  SATOH TAKATOMO ,  KANEKO YOSHIOKI ,  HATANAKA MIDORI ,  FUKUOKA MORINAO ,  SAKAMOTO HIROKO ,  YONEKAWA HIROYUKI

IPC: C12N15/09 ,  C12Q1/68 ,  G01N33/50 ,  G01N33/53 ,  G01N33/566 ,  G01N33/58

CPC classification number: C12Q1/6827 ,  C12Q1/6837 ,  C12Q2565/501 ,  C12Q2561/12 ,  C12Q2527/107

Abstract: It is intended to provide a method of detecting the data of a target nucleic acid comprising bringing the target nucleic acid into contact with a probe having a sequence being complementary to at leas a part of the target nucleic acid sequence to form a hybrid of the target nucleic acid with the probe, and measuring a signal generated in an amount depending on the amount of the hybrid to thereby detect the data of the target nucleic acid, wherein the signal data are kinetically obtained. It is also intended to provide a method of detecting the data of a target nucleic acid comprising bringing the target nucleic acid into contact with a completely matching probe being completely complementary to at leas a part of the target nucleic acid sequence and one or more incompletely matching probes having a variation in at least a part of the completely matching probe to form hybrids of the target nucleic acid with the completely matching probe and with the incompletely matching probe, and detecting the data of the target nucleic acid based on the difference in the binding strength between the hybrids, characterized in that the signal data are kinetically obtained while continuously or stepwise altering the conditions of measuring or detecting the signals generated from the hybrids.

公开(公告)号：EP2314677A4

公开(公告)日：2011-12-14

申请号：EP09800428

申请日：2009-07-23

Applicant: OLYMPUS CORP

Inventor： NAGAOKA TOMONORI ,  TANIGAMI YASUO

IPC: C12N15/00 ,  C12M1/00 ,  C12N15/09 ,  C12N15/10 ,  C12Q1/68

CPC classification number: C12N15/1003 ,  C12Q1/6806 ,  C12Q1/6883 ,  C12Q2527/101 ,  C12Q2527/125 ,  C12Q2600/154

公开(公告)号：EP3018480A4

公开(公告)日：2017-02-08

申请号：EP14819349

申请日：2014-04-15

Applicant: OLYMPUS CORP

Inventor： TAKEICHI RIE ,  MORIYA NAO ,  SANUKI HIROMI ,  NAKATA MARI ,  KIYOHARA MASANOBU ,  TAKEYAMA TETSUHIDE ,  NAGAOKA TOMONORI

IPC: G01N33/72 ,  A61B5/00 ,  A61B5/103 ,  A61B10/00 ,  G01J3/42 ,  G01J3/46 ,  G01N21/29 ,  G01N21/31 ,  G01N33/48 ,  G01N33/50 ,  G01N33/52 ,  G01N33/574 ,  G01N33/68

CPC classification number: G01N33/57438 ,  A61B5/1032 ,  A61B5/4283 ,  A61B2010/0061 ,  G01N21/29 ,  G01N21/293 ,  G01N21/31 ,  G01N33/48 ,  G01N33/6872 ,  G01N2333/70503 ,  G01N2800/067

Abstract: Provided are a method for exclusively selecting a duodenal fluid sample having a high possibility of containing pancreatic fluid and favorable sample suitability for subjecting to detection of a pancreatic disease marker by evaluating the quality of the sample prior to detecting the pancreatic disease marker, and a method for detecting a pancreatic disease marker using a duodenal fluid sample selected according to that method. Namely, a method is provided for selecting a duodenal fluid sample for detecting a pancreatic disease marker, comprising: (a1) a step of comparing the color depth of a duodenal fluid sample with a prescribed standard color, and (b1) a step of determining that a duodenal fluid sample is subjected to a test for a pancreatic disease marker if the color depth thereof is equal to or higher than the standard color, but that the duodenal fluid sample is not subjected to a test for a pancreatic disease marker if the color depth thereof is lower than the standard color.

公开(公告)号：EP2338989A4

公开(公告)日：2012-06-06

申请号：EP09809902

申请日：2009-08-25

Applicant: OLYMPUS CORP

Inventor： TANIGAMI YASUO ,  NAGAOKA TOMONORI

IPC: C12N15/09 ,  C12Q1/68

CPC classification number: C12Q1/6806 ,  C12Q2527/125

公开(公告)号：EP2218792A4

公开(公告)日：2010-12-08

申请号：EP08851485

申请日：2008-11-19

Applicant: OLYMPUS CORP

Inventor： TANIGAMI YASUO ,  NAGAOKA TOMONORI

IPC: C12Q1/68 ,  C12N15/00 ,  G01N1/28 ,  G01N33/48

CPC classification number: C12Q1/6806 ,  G01N1/38

公开(公告)号：EP1748082A4

公开(公告)日：2008-08-13

申请号：EP05736968

申请日：2005-04-27

Applicant: OLYMPUS CORP

Inventor： NAGAOKA TOMONORI

IPC: C12Q1/68 ,  C12N15/09 ,  G01N21/78 ,  G01N33/53 ,  G01N33/543 ,  G01N33/566 ,  G01N33/58 ,  G01N37/00

CPC classification number: C12Q1/6832 ,  C12Q2545/101

Abstract: It is intended to avoid any possibility of error attributed to a nucleic acid used as a standard, a probe or probe spot, etc. or enable correction thereof, to analyze the frequency of gene expression with high reproducibility and to analyze any change of primary structure in a sample nucleic acid. There is provided a method of analyzing the primary structure of at least one target base sequence present in a sample nucleic acid by detecting any signal from a sample nucleic acid hybrid composed of a probe and the sample nucleic acid, wherein any signal from a synthetic standard nucleic acid hybrid composed of the probe and a synthetic standard nucleic acid containing the at least one target base sequence is detected and on the basis of the signal from the synthetic standard nucleic acid hybrid, the signal from the sample nucleic acid hybrid is corrected to thereby effect analysis of the primary structure of the sample nucleic acid.

公开(公告)号：JP2012143212A

公开(公告)日：2012-08-02

申请号：JP2011005724

申请日：2011-01-14

Applicant: Olympus Corp ,  オリンパス株式会社

Inventor： HIRAGA CHIHIRO ,  NAGAOKA TOMONORI

IPC: C12M1/00 ,  C12N15/00 ,  G01N1/10 ,  G01N1/16

Abstract: PROBLEM TO BE SOLVED: To recover only a lower layer among a plurality of layers subjected to layer separation with a simple configuration and operation.SOLUTION: The solution recovery auxiliary tool 1 is used when the lower layer is suctioned by a suction tube out of an upper layer and the lower layer subjected to layer separation in a container 2, and includes: a cylindrical barrel part 11 which has a through-hole 11a being open at both ends, can be inserted from one end side in the container 2, and can insert the suction tube in the through-hole 11a; a sealing member 12 which can be penetrated from a tip end of the suction tube by tightly sealing the opening at one end of the barrel part 11; and a shoulder part 13 which is provided by projecting outside on an outer peripheral surface of the barrel part 11, and is abutted with an upper surface of the container 2 when the barrel part 11 is inserted in the container 2.

Abstract translation: 要解决的问题：仅通过简单的构造和操作在仅进行层分离的多个层中仅回收下层。 解决方案：当下层由上层抽吸的管抽出并且下层在容器2中进行层分离时，使用溶液回收辅助工具1，其包括：圆筒形管部分11， 具有在两端开口的通孔11a，可以从容器2的一端侧插入，并且能够将吸引管插入到通孔11a中; 密封构件12，其可以通过紧密地密封在筒部11的一端处的开口而从吸入管的尖端穿透; 以及肩部13，其在筒部11的外周面上向外侧突出设置，并且当筒部11插入容器2中时与该容器2的上表面抵接。背景技术： （C）2012，JPO＆INPIT

公开(公告)号：JP2010008106A

公开(公告)日：2010-01-14

申请号：JP2008164961

申请日：2008-06-24

Applicant: Olympus Corp ,  オリンパス株式会社

Inventor： NAKAJIMA KAZUE ,  SHIBAZAKI TAKAMI ,  NAKAMOTO MARI ,  FUKUOKA MORINAO ,  NAGAOKA TOMONORI

IPC: G01N1/04 ,  G01N1/10

Abstract: PROBLEM TO BE SOLVED: To provide a feces treatment method and a feces treatment container capable of performing stable nucleic acid recovery. SOLUTION: In this feces treatment container 1 equipped with a feces sampling tool 100, a suspension holding part 110 and a treating liquid holding part 120, wherein a feces sample preparing solution S is stored in a solution holding container 121, a feces sample E collected by the feces sampling tool 100 is adjusted into a fixed amount by scraping off excessive feces by a guide part 112 when the feces sampling tool 100 is fitted to a feces holding part 110. Thereafter, by pressing the feces sample E by a piston 103, the feces sample E is shredded and dispersed into feces holding container 111 by a dispersion member 113. By pressing the feces holding container 111 into the solution holding container 121, a projection part 115 drills and breaks a sealing film 122, to thereby mix the dispersed feces sample E with the feces sample preparing solution S. The feces sample E is shredded and dispersed, to thereby enable stable nucleic acid recovery. COPYRIGHT: (C)2010,JPO&INPIT

Abstract translation: 待解决的问题：提供能够进行稳定的核酸回收的粪便处理方法和粪便处理容器。 解决方案：在具有粪便取样工具100的悬浮液保持部110和处理液保持部120的粪便处理容器1中，将粪便试样调制溶液S储存在溶液保持容器121中，将粪便 当粪便采样工具100装配到粪便保持部分110时，通过将粪便采样工具100收集的样品E调整为固定量，通过引导部分112刮除多余的粪便。之后，通过将粪便样本E 活塞103将粪便样品E切碎并通过分散构件113分散在粪便保持容器111中。通过将粪便保持容器111压入溶液保持容器121中，突出部分115钻并破坏密封膜122，由此 将分散的粪便样品E与粪便样品制备溶液S混合。将粪便样品E切碎并分散，从而使稳定的核酸回收。 版权所有（C）2010，JPO＆INPIT

公开(公告)号：JP2009165401A

公开(公告)日：2009-07-30

申请号：JP2008006761

申请日：2008-01-16

Applicant: Olympus Corp ,  オリンパス株式会社

Inventor： NAGAOKA TOMONORI

IPC: C12Q1/68 ,  C12N15/09

Abstract: PROBLEM TO BE SOLVED: To provide a method for determining a target substance by which the ratio of the amounts of the target substance in samples can be determined more accurately by properly compensating the dispersion of measured results caused by the difference in initial qualities of samples, and the influence of analyzing operation or the like when determining the masses of the target substance in the two or more samples containing living body-associated substances of two or more kinds of living body species.
SOLUTION: The method for determining the target substance in each of two or more samples containing a reference material different from the target substance and being a living body-associated substance of a living body species different from the target substance includes (a) a step for obtaining the ratio of the amounts of the reference material in each sample by measuring the amount of the reference material in each sample, (b) a step for measuring the amount of the target substance in each sample, and (c) a step for compensating the amount of the target substance in each sample obtained in the step (b) by using the ratio of the amounts of the reference material in each sample obtained in the step (a).
COPYRIGHT: (C)2009,JPO&INPIT

Abstract translation: 要解决的问题：提供一种用于确定目标物质的方法，通过适当地补偿由初始质量差引起的测量结果的分散，可以更精确地确定样品中目标物质的量的比例 以及在两种以上的活体物种的生物体相关物质的两个以上的样品中测定目标物质的质量时的分析手术等的影响。 含有不同于目标物质的参考物质和与目标物质不同的活体物质的两种以上试样中的目标物质的测定方法包括：（a） 通过测量每个样品中的参考物质的量来获得每个样品中的参考物质的量的比例的步骤，（b）测量每个样品中目标物质的量的步骤，和（c） 通过使用步骤（a）中获得的每个样品中的参考物质的量的比例来补偿步骤（b）中获得的每个样品中的目标物质的量的步骤。 版权所有（C）2009，JPO＆INPIT