公开(公告)号：WO03062418B1

公开(公告)日：2004-04-01

申请号：PCT/JP0300668

申请日：2003-01-24

Applicant: OLYMPUS CORP ,  KOIKE HISASHI ,  NAGAOKA TOMONORI ,  SATOH TAKATOMO ,  KANEKO YOSHIOKI ,  HATANAKA MIDORI ,  FUKUOKA MORINAO ,  SAKAMOTO HIROKO ,  YONEKAWA HIROYUKI

Inventor： KOIKE HISASHI ,  NAGAOKA TOMONORI ,  SATOH TAKATOMO ,  KANEKO YOSHIOKI ,  HATANAKA MIDORI ,  FUKUOKA MORINAO ,  SAKAMOTO HIROKO ,  YONEKAWA HIROYUKI

IPC: C12N15/09 ,  C12Q1/68 ,  G01N33/50 ,  G01N33/53 ,  G01N33/566 ,  G01N33/58

CPC classification number: C12Q1/6827 ,  C12Q1/6837 ,  C12Q2565/501 ,  C12Q2561/12 ,  C12Q2527/107

Abstract: It is intended to provide a method of detecting the data of a target nucleic acid comprising bringing the target nucleic acid into contact with a probe having a sequence being complementary to at leas a part of the target nucleic acid sequence to form a hybrid of the target nucleic acid with the probe, and measuring a signal generated in an amount depending on the amount of the hybrid to thereby detect the data of the target nucleic acid, wherein the signal data are kinetically obtained. It is also intended to provide a method of detecting the data of a target nucleic acid comprising bringing the target nucleic acid into contact with a completely matching probe being completely complementary to at leas a part of the target nucleic acid sequence and one or more incompletely matching probes having a variation in at least a part of the completely matching probe to form hybrids of the target nucleic acid with the completely matching probe and with the incompletely matching probe, and detecting the data of the target nucleic acid based on the difference in the binding strength between the hybrids, characterized in that the signal data are kinetically obtained while continuously or stepwise altering the conditions of measuring or detecting the signals generated from the hybrids.

Abstract translation: 旨在提供一种检测目标核酸的数据的方法，包括使靶核酸与具有与靶核酸序列的一部分互补的序列的探针接触，以形成目标核酸的杂交体 核酸，并且测量根据混合物的量产生的量的信号，从而检测目标核酸的数据，其中信号数据被动力学地获得。 还旨在提供一种检测靶核酸的数据的方法，包括使靶核酸与完全匹配的探针完全互补，所述完全匹配的探针与目标核酸序列的一部分完全互补，并且一个或多个不完全匹配 探针在完全匹配的探针的至少一部分上具有变异，以与完全匹配的探针和不完全匹配的探针形成靶核酸的杂交体，并且基于结合的差异检测靶核酸的数据 其特征在于，在连续或逐步地改变测量或检测从杂种产生的信号的条件下动态获得信号数据。

公开(公告)号：EP1420250A4

公开(公告)日：2006-03-22

申请号：EP02755725

申请日：2002-07-31

Applicant: OLYMPUS CORP

Inventor： YONEKAWA HIROYUKI ,  SHIBAZAKI TAKAMI ,  SATOH TAKATOMO ,  SAKAMOTO HIROKO

IPC: B01L3/00 ,  C12M1/00 ,  C12M1/34 ,  C12N15/09 ,  C12Q1/68 ,  C40B40/06 ,  G01N33/53 ,  G01N37/00

CPC classification number: B01L3/50255 ,  B01J2219/00608 ,  B01J2219/00612 ,  B01J2219/00641 ,  B01J2219/00722 ,  B01L2300/0819 ,  B01L2400/0487 ,  C40B40/06

Abstract: A gene inspection apparatus includes (1) a DNA micro array consisting of a plenty of small liquid vessels containing liquid three-dimensionally and arranged in such a manner that their openings are arranged in one plane two-dimensionally wherein a hybridization reaction is performed between target nucleic acid marked in advance by an optical marker substance and the nucleic acid probe, and (2) a microscope having a stage for supporting the DNA micro array described in (1), a temperature adjusting unit for adjusting the temperature of the DNA micro array, and image pickup means for imaging an optical signal from the DNA micro array.

公开(公告)号：EP1469067A4

公开(公告)日：2007-05-23

申请号：EP03703050

申请日：2003-01-24

Applicant: OLYMPUS CORP

Inventor： KOIKE HISASHI ,  NAGAOKA TOMONORI ,  SATOH TAKATOMO ,  KANEKO YOSHIOKI ,  HATANAKA MIDORI ,  FUKUOKA MORINAO ,  SAKAMOTO HIROKO ,  YONEKAWA HIROYUKI

IPC: C12N15/09 ,  C12Q1/68 ,  G01N33/50 ,  G01N33/53 ,  G01N33/566 ,  G01N33/58

CPC classification number: C12Q1/6827 ,  C12Q1/6837 ,  C12Q2565/501 ,  C12Q2561/12 ,  C12Q2527/107

Abstract: It is intended to provide a method of detecting the data of a target nucleic acid comprising bringing the target nucleic acid into contact with a probe having a sequence being complementary to at leas a part of the target nucleic acid sequence to form a hybrid of the target nucleic acid with the probe, and measuring a signal generated in an amount depending on the amount of the hybrid to thereby detect the data of the target nucleic acid, wherein the signal data are kinetically obtained. It is also intended to provide a method of detecting the data of a target nucleic acid comprising bringing the target nucleic acid into contact with a completely matching probe being completely complementary to at leas a part of the target nucleic acid sequence and one or more incompletely matching probes having a variation in at least a part of the completely matching probe to form hybrids of the target nucleic acid with the completely matching probe and with the incompletely matching probe, and detecting the data of the target nucleic acid based on the difference in the binding strength between the hybrids, characterized in that the signal data are kinetically obtained while continuously or stepwise altering the conditions of measuring or detecting the signals generated from the hybrids.