公开(公告)号：WO2009145828A2

公开(公告)日：2009-12-03

申请号：PCT/US2009002003

申请日：2009-03-30

Applicant: PACIFIC BIOSCIENCES CALIFORNIA ,  BJORNSON KEITH ,  LEE HAROLD ,  KORLACH JONAS ,  TURNER STEPHEN ,  BIBILLO AREK ,  CHRISTIANS FRED ,  EMIG ROBIN ,  HE MOLLY ,  PARK INSIL ,  JIA LEI ,  FEDOROV ANDREI ,  LYLE JOHN

Inventor： BJORNSON KEITH ,  LEE HAROLD ,  KORLACH JONAS ,  TURNER STEPHEN ,  BIBILLO AREK ,  CHRISTIANS FRED ,  EMIG ROBIN ,  HE MOLLY ,  PARK INSIL ,  JIA LEI ,  FEDOROV ANDREI ,  LYLE JOHN

IPC: C12Q1/68 ,  C12N9/12 ,  C12Q1/48

CPC classification number: C12Q1/6869 ,  C12Q1/6874 ,  C12Q2527/125 ,  C12Q2527/119 ,  C12Q2527/101 ,  C12Q2565/1015 ,  C12Q2527/137 ,  C12Q2521/101

Abstract: Compositions, kits, methods and systems for nucleotide sequencing comprising producing polymerase reactions that exhibit two kinetically observable steps within an observable phase of the polymerase reaction. Two slow step systems can be produced, for example, by selecting the appropriate polymerase enzyme, polymerase reaction conditions including cofactors, and polymerase reaction substrates including the primed template and nucleotides.

Abstract translation: 用于核苷酸测序的组合物，试剂盒，方法和系统，其包括产生聚合酶反应，其在聚合酶反应的可观察期内显示两个动力学可观察步骤。 可以通过例如选择合适的聚合酶，包括辅因子的聚合酶反应条件和包括引发的模板和核苷酸的聚合酶反应底物来产生两个缓慢的步骤系统。

公开(公告)号：WO2012021733A3

公开(公告)日：2012-08-02

申请号：PCT/US2011047468

申请日：2011-08-11

Applicant: PACIFIC BIOSCIENCES CALIFORNIA ,  YUE STEPHEN ,  WEBER ROBERT ,  KONG XIANGXU ,  FEDOROV ANDREI ,  LYLE JOHN

Inventor： YUE STEPHEN ,  WEBER ROBERT ,  KONG XIANGXU ,  FEDOROV ANDREI ,  LYLE JOHN

IPC: G01N33/52 ,  C12Q1/25 ,  C12Q1/68

CPC classification number: C12Q1/6869 ,  C12N13/00 ,  C12Q1/25 ,  G01N33/52 ,  G01N33/54373 ,  G01N2333/91245 ,  G01N2333/916

Abstract: Compositions, devices, systems and methods for reducing and/or preventing photo-induced damage of one or more reactants in an illuminated analytical reaction by addition of one or more photoprotective compounds to the reaction mixture and allowing the reaction to proceed for a period that is less than a photo-induced damage threshold period.

Abstract translation: 用于通过向反应混合物中加入一种或多种光保护性化合物并允许反应进行一段时间，以减少和/或防止照明分析反应中的一种或多种反应物的光诱导损伤的组合物，装置，系统和方法 小于光致伤害阈值期。

公开(公告)号：WO2012009206A2

公开(公告)日：2012-01-19

申请号：PCT/US2011043227

申请日：2011-07-07

Applicant: PACIFIC BIOSCIENCES CALIFORNIA ,  FEDOROV ANDREI ,  LYLE JOHN ,  BJORNSON KEITH ,  HANES JEREMIAH

Inventor： FEDOROV ANDREI ,  LYLE JOHN ,  BJORNSON KEITH ,  HANES JEREMIAH

IPC: C12Q1/68 ,  C12N15/10 ,  C12N15/11 ,  G01N33/84

CPC classification number: C12Q1/6869 ,  C12Q2527/125 ,  C12Q2563/137

Abstract: Compositions, kits, methods and systems for single molecule nucleotide sequencing comprising producing polymerase reactions having monovalent cations that control the median pulse width for incorporated nucleotides are disclosed. The levels of alkali metals such as lithium, sodium, potassium, rubidium, and cesium in the polymerization are used to control pulse width while allowing other sequencing parameters to remain within a desirable range.

Abstract translation: 公开了用于单分子核苷酸测序的组合物，试剂盒，方法和系统，其包括产生具有控制掺入的核苷酸的中值脉冲宽度的具有一价阳离子的聚合酶反应。 聚合中的锂，钠，钾，铷和铯等碱金属的含量被用于控制脉冲宽度，同时允许其它测序参数保持在期望的范围内。

公开(公告)号：EP3030683A4

公开(公告)日：2017-05-03

申请号：EP14834734

申请日：2014-08-05

Applicant: PACIFIC BIOSCIENCES CALIFORNIA INC

Inventor： SEBO LUBOMIR ,  HANES JEREMIAH ,  SHEN GENE ,  BROGLEY LOUIS ,  YUE STEPHEN ,  ZHENG FRANK ,  LAPIN YURI ,  LYLE JOHN ,  OSUNA HONEY ,  FEDOROV ANDREI

IPC: C12Q1/68 ,  C07F9/6558 ,  C07F9/6561 ,  C07H19/10 ,  G01N21/64 ,  G01N23/223

CPC classification number: C07H19/10 ,  C07F9/65586 ,  C07F9/65616

Abstract: Protected fluorescent reagent compounds and their methods of synthesis are provided. The compounds are useful in various fluorescence-based analytical methods, including the analysis of highly multiplexed optical reactions in large numbers at high densities, such as single molecule real time nucleic acid sequencing reactions. The compounds contain fluorescent dye elements, that allow the compounds to be detected with high sensitivity at desirable wavelengths, binding elements, that allow the compounds to be recognized specifically by target biomolecules, and protective shield elements, that decrease undesirable contacts between the fluorescent dye elements and the bound target biomolecules and that therefore decrease photodamage of the bound target biomolecules by the fluorescent dye elements.

Abstract translation: 提供受保护的荧光试剂化合物及其合成方法。 这些化合物可用于各种基于荧光的分析方法，包括高密度分析大量高度多重光学反应，如单分子实时核酸测序反应。 这些化合物含有荧光染料元素，可以在理想的波长下以高灵敏度检测化合物，允许化合物特异性地被目标生物分子识别的结合元件和保护性屏蔽元​​件，其减少荧光染料元素之间的不希望的接触 和结合的目标生物分子，并因此减少了荧光染料元件对结合的目标生物分子的光损伤。

公开(公告)号：EP2274446A4

公开(公告)日：2011-04-20

申请号：EP09755191

申请日：2009-03-30

Applicant: PACIFIC BIOSCIENCES CALIFORNIA

Inventor： BJORNSON KEITH ,  LEE HAROLD ,  KORLACH JONAS ,  TURNER STEPHEN ,  BIBILLO AREK ,  CHRISTIANS FRED ,  EMIG ROBIN ,  HE MOLLY ,  PARK INSIL ,  JIA LEI ,  FEDOROV ANDREI ,  LYLE JOHN M

IPC: C12Q1/68 ,  C12N9/12 ,  C12Q1/48

CPC classification number: C12Q1/6869 ,  C12Q1/6874 ,  C12Q2527/125 ,  C12Q2527/119 ,  C12Q2527/101 ,  C12Q2565/1015 ,  C12Q2527/137 ,  C12Q2521/101