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公开(公告)号：WO2009145828A2
公开(公告)日：2009-12-03
申请号：PCT/US2009002003
申请日：2009-03-30
Applicant: PACIFIC BIOSCIENCES CALIFORNIA , BJORNSON KEITH , LEE HAROLD , KORLACH JONAS , TURNER STEPHEN , BIBILLO AREK , CHRISTIANS FRED , EMIG ROBIN , HE MOLLY , PARK INSIL , JIA LEI , FEDOROV ANDREI , LYLE JOHN
Inventor： BJORNSON KEITH , LEE HAROLD , KORLACH JONAS , TURNER STEPHEN , BIBILLO AREK , CHRISTIANS FRED , EMIG ROBIN , HE MOLLY , PARK INSIL , JIA LEI , FEDOROV ANDREI , LYLE JOHN
CPC classification number: C12Q1/6869 , C12Q1/6874 , C12Q2527/125 , C12Q2527/119 , C12Q2527/101 , C12Q2565/1015 , C12Q2527/137 , C12Q2521/101
Abstract: Compositions, kits, methods and systems for nucleotide sequencing comprising producing polymerase reactions that exhibit two kinetically observable steps within an observable phase of the polymerase reaction. Two slow step systems can be produced, for example, by selecting the appropriate polymerase enzyme, polymerase reaction conditions including cofactors, and polymerase reaction substrates including the primed template and nucleotides.
Abstract translation: 用于核苷酸测序的组合物，试剂盒，方法和系统，其包括产生聚合酶反应，其在聚合酶反应的可观察期内显示两个动力学可观察步骤。 可以通过例如选择合适的聚合酶，包括辅因子的聚合酶反应条件和包括引发的模板和核苷酸的聚合酶反应底物来产生两个缓慢的步骤系统。
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2.发明申请GENERATION OF MODIFIED POLYMERASES FOR IMPROVED ACCURACY IN SINGLE MOLECULE SEQUENCING 审中-公开在单分子序列中改进的改进聚合物的生成
公开(公告)号：WO2011040971A2
公开(公告)日：2011-04-07
申请号：PCT/US2010002659
申请日：2010-09-30
Applicant: PACIFIC BIOSCIENCES CALIFORNIA , CLARK SONYA , BIBILLO AREK , PELUSO PAUL , CHRISTIANS FRED , HE MOLLY , PARK INSIL , LEE HAROLD , BJORNSON KEITH , JIA LEI , EMIG ROBIN
Inventor： CLARK SONYA , BIBILLO AREK , PELUSO PAUL , CHRISTIANS FRED , HE MOLLY , PARK INSIL , LEE HAROLD , BJORNSON KEITH , JIA LEI , EMIG ROBIN
CPC classification number: C12N9/1252 , C12Q1/6869 , C12Q2521/101
Abstract: Provided are compositions comprising recombinant DNA polymerases that include amino acid substitutions, insertions, deletions and/or heterologous or exogenous features that confer modified properties upon the polymerase for enhanced single molecule sequencing. Such properties can include reduced reaction rates at one or more steps of the polymerase kinetic cycle, increased closed polymerase/DNA complex stability, enhanced metal ion coordination, reduced exonuclease activity, decreased branching fractions, and the like. Also provided are nucleic acids which encode the polymerases with the aforementioned phenotypes, as well as methods of using such polymerases to make a DNA or to sequence a DNA template.
Abstract translation: 提供了包含重组DNA聚合酶的组合物，其包括氨基酸取代，插入，缺失和/或异源或外源特征，其在聚合酶上赋予改进的性质以用于增强的单分子测序。 这种性质可以包括聚合酶动力学循环的一个或多个步骤的降低的反应速率，增加的闭合聚合酶/ DNA复合物稳定性，增强的金属离子配位，减少的核酸外切酶活性，降低的支化部分等。 还提供了编码具有上述表型的聚合酶的核酸，以及使用这种聚合酶制备DNA或顺序DNA模板的方法。
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公开(公告)号：CA2720046C
公开(公告)日：2018-07-24
申请号：CA2720046
申请日：2009-03-30
Applicant: PACIFIC BIOSCIENCES CALIFORNIA INC
Inventor： CLARK SONYA , BIBILLO AREK , PELUSO PAUL , CHRISTIANS FRED , HE MOLLY , PARK INSIL , LEE HAROLD , BJORNSON KEITH , JIA LEI , EMIG ROBIN
Abstract: Provided are compositions comprising modified recombinant polymerases that exhibit branching fractions that are less than the branching fractions of the polymerases from which they were derived, or branching fractions that are less than about 25% for a phosphate-labeled nucleotide analog. Also provided are compositions comprising modified recombinant polymerases that exhibit closed polymerase/DNA complexes with increased stability relative to the parental polymerases. Also provided are compositions comprising modified recombinant polymerases that exhibit decreased rate constants relative to the parental polymerases. Provided are methods for generating polymerases with the aforementioned phenotypes. Provided are methods of using such polymerases to make a DNA or to sequence a DNA template.
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公开(公告)号：ES2648313T3
公开(公告)日：2017-12-29
申请号：ES16157104
申请日：2006-12-21
Applicant: PACIFIC BIOSCIENCES CALIFORNIA INC
Inventor： HANZEL DAVID , OTTO GEOFF , MURPHY DEVON , PELUSO PAUL , PHAM THANG , RANK DAVID , MITSIS PAUL , CHRISTIANS FRED , BIBILLO AREK , PARK INSIL , CLARK SONYA , LYLE JOHN
IPC: C12Q1/68
Abstract: Una composición que comprende: una ADN polimerasa recombinante, comprendiendo dicha polimerasa recombinante una secuencia de aminoácidos que es al menos un 70 % idéntica a la SEQ ID NO:1 y comprende una sustitución de aminoácido en un sitio correspondiente a K512 respecto a la SEQ ID NO:1; y un análogo nucleotídico que está marcado en un grupo fosfato, en el que el resto de base del análogo se selecciona del grupo que consiste en adenina, timina, guanina y citidina.
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公开(公告)号：AU2008282862B2
公开(公告)日：2014-07-31
申请号：AU2008282862
申请日：2008-07-25
Applicant: PACIFIC BIOSCIENCES CALIFORNIA INC
Inventor： CHRISTIANS FRED , TURNER STEPHEN
Abstract: Methods, systems and compositions where a target nucleic acid includes a registration sequence disposed therein for identification of the number or relative position of determined sequence from the template sequence. Particularly preferred aspects include a registration sequence in a circular template nucleic acid sequence which is, in turn, used in sequence by incorporation processes that rely upon template dependent, polymerase mediated primer extension in the identification of the sequence of the template.
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Patent Agency Ranking
公开(公告)号：CA2720046A1
公开(公告)日：2009-12-03
申请号：CA2720046
申请日：2009-03-30
Applicant: PACIFIC BIOSCIENCES CALIFORNIA
Inventor： CLARK SONYA , BIBILLO AREK , PELUSO PAUL , CHRISTIANS FRED , HE MOLLY , PARK INSIL , LEE HAROLD , BJORNSON KEITH , JIA LEI , EMIG ROBIN
Abstract: Provided are compositions comprising modified recombinant polymerases that exhibit branching fractions that are less than the branching fractions of the polymerases from which they were derived, or branching fractions that are less than about 25% for a phosphate-labeled nucleotide analog. Also provided are compositions comprising modified recombinant polymerases that exhibit closed polymerase/DNA complexes with increased stability relative to the parental polymerases. Also provided are compositions comprising modified recombinant polymerases that exhibit decreased rate constants relative to the parental polymerases. Provided are methods for generating polymerases with the aforementioned phenotypes. Provided are methods of using such polymerases to make a DNA or to sequence a DNA template.
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公开(公告)号：CA2693979A1
公开(公告)日：2009-02-05
申请号：CA2693979
申请日：2008-07-25
Applicant: PACIFIC BIOSCIENCES CALIFORNIA
Inventor： CHRISTIANS FRED , TURNER STEPHEN
Abstract: Methods, systems and compositions where a target nucleic acid includes a registration sequence disposed therein for identification of the number or relative position of determined sequence from the template sequence. Particularly preferred aspects include a registration sequence in a circular template nucleic acid sequence which is, in turn, used in sequence by incorporation processes that rely upon template dependent, polymerase mediated primer extension in the identification of the sequence of the template.
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公开(公告)号：CA2720247A1
公开(公告)日：2009-12-03
申请号：CA2720247
申请日：2009-03-30
Applicant: PACIFIC BIOSCIENCES CALIFORNIA
Inventor： EID JOHN , TURNER STEPHEN , DALAL RAVI , FLUSBERG BENJAMIN , KORLACH JONAS , LIN STEVEN , FEHR ADRIAN , CHRISTIANS FRED , EMIG ROBIN
Abstract: Methods, compositions and arrays for non-random loading of single analyte molecules into array structures are provided.
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公开(公告)号：AU2009251881A1
公开(公告)日：2009-12-03
申请号：AU2009251881
申请日：2009-03-30
Applicant: PACIFIC BIOSCIENCES CALIFORNIA
Inventor： CHRISTIANS FRED , KORLACH JONAS , DALAL RAVI , EID JOHN , LIN STEVEN , FEHR ADRIAN , EMIG ROBIN , FLUSBERG BENJAMIN , TURNER STEPHEN
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公开(公告)号：CA2720247C
公开(公告)日：2020-07-14
申请号：CA2720247
申请日：2009-03-30
Applicant: PACIFIC BIOSCIENCES CALIFORNIA INC
Inventor： EID JOHN , TURNER STEPHEN , DALAL RAVI , FLUSBERG BENJAMIN , KORLACH JONAS , LIN STEVEN , FEHR ADRIAN , CHRISTIANS FRED , EMIG ROBIN
Abstract: Methods, compositions and arrays for non-random loading of single analyte molecules into array structures are provided.
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