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公开(公告)号：WO2010117420A3
公开(公告)日：2011-03-24
申请号：PCT/US2010000948
申请日：2010-03-29
Applicant: PACIFIC BIOSCIENCES CALIFORNIA , KONG XIANGXU , SHEN GENE
Inventor： KONG XIANGXU , SHEN GENE
CPC classification number: C12Q1/6818 , C07H19/207 , C12Q1/6869 , G01N21/6428 , G01N33/542 , G01N33/582 , G01N2021/6441 , Y10T436/143333
Abstract: FRET-labeled compounds are provided for use in analytical reactions. In certain embodiments, FRET-labeled nucleotide analogs are used in place of naturally occurring nucleoside triphosphates or other analogs in analytical reactions comprising nucleic acids, for example, template-directed nucleic acid synthesis, DNA sequencing, RNA sequencing, single-base identification, hybridization, binding assays, and other analytical reactions.
Abstract translation: 提供FRET标记的化合物用于分析反应。 在某些实施方案中，FRET标记的核苷酸类似物用于代替包含核酸的分析反应中天然存在的核苷三磷酸或其它类似物，例如模板指导的核酸合成，DNA测序，RNA测序，单碱基鉴定，杂交 ，结合测定和其他分析反应。
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公开(公告)号：CA2714630A1
公开(公告)日：2009-08-13
申请号：CA2714630
申请日：2009-02-06
Applicant: PACIFIC BIOSCIENCES CALIFORNIA
Inventor： OTTO GEOFFREY , SHEN GENE , KONG XIANGXU , EMIG ROBIN
Abstract: The present invention provides methods and compositions for performing illuminated reactions, particularly sequencing reactions, while mitigating and/or preventing photodamage to reactants that can result from prolonged illumination. In particular, the invention provides methods and compositions for incorporating photoprotective agents into conjugates comprising reporter molecules and nucleoside polyphosphates.
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公开(公告)号：AU2007260707A1
公开(公告)日：2007-12-21
申请号：AU2007260707
申请日：2007-06-15
Applicant: PACIFIC BIOSCIENCES CALIFORNIA INC
Inventor： SHEN GENE , PELUSO PAUL , LYLE JOHN
IPC: C12Q1/68
Abstract: Controlled initiation of primer extension in determination of nucleic acid sequence information by incorporation of nucleotides or nucleotide analogs. Preferred aspects include photo-initiated extension through the use of photo-cleavable blocking, groups on termini of primer sequences followed by non-terminating primer extension using nucleotides or nucleotide analogs that are not extension terminators.
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公开(公告)号：AU2009212165A1
公开(公告)日：2009-08-13
申请号：AU2009212165
申请日：2009-02-06
Applicant: PACIFIC BIOSCIENCES CALIFORNIA INC
Inventor： SHEN GENE , KONG XIANGXU , EMIG ROBIN , OTTO GEOFFREY
IPC: C07H21/00
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公开(公告)号：CA2655018A1
公开(公告)日：2007-12-21
申请号：CA2655018
申请日：2007-06-15
Applicant: PACIFIC BIOSCIENCES CALIFORNIA
Inventor： SHEN GENE , PELUSO PAUL , LYLE JOHN
Abstract: Controlled initiation of primer extension in determination of nucleic aci d sequence information by incorporation of nucleotides or nucleotide analogs . Preferred aspects include photo-initiated extension through the use of pho to-cleavable blocking groups on termini of primer sequences followed by non- terminating primer extension using nucleotides or nucleotide analogs that ar e not extension terminators.
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Patent Agency Ranking
公开(公告)号：WO2012027625A3
公开(公告)日：2012-06-28
申请号：PCT/US2011049249
申请日：2011-08-25
Applicant: PACIFIC BIOSCIENCES CALIFORNIA , SHEN GENE , SEBO LUBOMIR , YUE STEPHEN , SUN DAVID , BROGLEY LOUIS , FEDEROV ANDREI
Inventor： SHEN GENE , SEBO LUBOMIR , YUE STEPHEN , SUN DAVID , BROGLEY LOUIS , FEDEROV ANDREI
IPC: C12N11/00 , C07D251/12 , C07D403/04 , C12Q1/25 , C12Q1/68 , G01N33/52
CPC classification number: C12Q1/6869 , C07D209/14 , C07D401/14 , C07D405/14 , C07H19/10 , C07K5/0815 , C07K5/1019 , C09B23/0025 , C09B23/0033 , C09B23/0066 , C09B23/06 , C09B23/083 , C09B23/105 , C09B23/164 , C12Q1/6844 , C12Q1/6876 , G01N33/52 , G01N33/582 , G01N2333/9126
Abstract: The invention provides a novel class of scaffold-based labeled polymerase enzyme substrates. The polymerase enzyme substrates have a multivalent core or scaffold to which is attached fluorescent dye moieties and nucleoside phosphate moities. The polymerase enzyme substrates have multiple fluorescent dye moities and/or multiple nucleoside phosphate moieties. Preferred multivalent cores comprise trifunctional six membered aromatic moities. The invention also provides for sequencing methods and kits with scaffold-based labeled polymerase enzyme substrates.
Abstract translation: 本发明提供了一类新型的基于脚手架的标记聚合酶酶底物。 聚合酶底物具有连接荧光染料部分和核苷磷酸部分的多价核心或支架。 聚合酶底物具有多个荧光染料部分和/或多个核苷磷酸部分。 优选的多价核心包含三官能六元芳族部分。 本发明还提供了具有基于脚手架的标记的聚合酶底物的测序方法和试剂盒。
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7.发明申请PHOTO-INDUCED DAMAGE MITIGATING AGENTS AND PREPARATION AND METHODS OF USE THEREOF 审中-公开光诱导损伤增强剂及其制备方法和使用方法
公开(公告)号：WO2010059880A2
公开(公告)日：2010-05-27
申请号：PCT/US2009065222
申请日：2009-11-19
Applicant: PACIFIC BIOSCIENCES CALIFORNIA , KONG XIANGXU , SHEN GENE , FEDEROV ANDREI , LYLE JOHN , LEE GRACE , SEBO LUBOMIR , DO DUC , WEBER ROBERT , DUDEK STEVE
Inventor： KONG XIANGXU , SHEN GENE , FEDEROV ANDREI , LYLE JOHN , LEE GRACE , SEBO LUBOMIR , DO DUC , WEBER ROBERT , DUDEK STEVE
CPC classification number: C07C49/255 , G01N21/6428 , G01N33/5306 , G01N2021/6432
Abstract: Compositions, devices, systems and methods for reducing and/or preventing photo-induced damage of one or more reactants in an illuminated analytical reaction by addition of one or more photo-induced damage mitigating agents to the reaction mixture and allowing the reaction to proceed for a period that is less than a photo-induced damage threshold period.
Abstract translation: 用于通过向反应混合物添加一种或多种光诱导损伤缓解剂并允许反应进行以减少和/或防止照射分析反应中一种或多种反应物的光诱导损伤的组合物，装置，系统和方法 小于光诱导损伤阈值时间的时间段。
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公开(公告)号：WO2012027623A3
公开(公告)日：2012-06-21
申请号：PCT/US2011049247
申请日：2011-08-25
Applicant: PACIFIC BIOSCIENCES CALIFORNIA , YUE STEPHEN , SHEN GENE , SUN DAVID
Inventor： YUE STEPHEN , SHEN GENE , SUN DAVID
IPC: C07D403/06 , C07D209/10 , C07D209/18 , C07D209/30 , C07D401/14 , C09B23/00
CPC classification number: C12Q1/6869 , C07D209/14 , C07D401/14 , C07D405/14 , C07H19/10 , C07K5/0815 , C07K5/1019 , C09B23/0025 , C09B23/0033 , C09B23/0066 , C09B23/06 , C09B23/083 , C09B23/105 , C09B23/164 , C12Q1/6844 , C12Q1/6876 , G01N33/52 , G01N33/582 , G01N2333/9126
Abstract: The invention provides a novel class of cyanine dyes that are functionalized with sulfonic acid groups and a linker moiety that facilitates their conjugation to other species and substituent groups which increase the water-solubility, and optimize the optical properties of the dyes. Also provided are conjugates of the dyes, methods of using the dyes and their conjugates and kits including the dyes and their conjugates.
Abstract translation: 本发明提供了一类新颖的花青染料，它们用磺酸基官能化，连接部分有助于它们与其它物质和取代基结合，这些物质和取代基增加了水溶性，并优化了染料的光学性质。 还提供了染料的缀合物，使用染料及其缀合物的方法和试剂盒，包括染料及其缀合物。
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公开(公告)号：WO2012027618A3
公开(公告)日：2012-05-18
申请号：PCT/US2011049242
申请日：2011-08-25
Applicant: PACIFIC BIOSCIENCES CALIFORNIA , SHEN GENE , YUE STEPHEN
Inventor： SHEN GENE , YUE STEPHEN
IPC: C07D403/06 , C07D209/10 , C07D209/18 , C07D209/30 , C09B23/00
CPC classification number: C12Q1/6869 , C07D209/14 , C07D401/14 , C07D405/14 , C07H19/10 , C07K5/0815 , C07K5/1019 , C09B23/0025 , C09B23/0033 , C09B23/0066 , C09B23/06 , C09B23/083 , C09B23/105 , C09B23/164 , C12Q1/6844 , C12Q1/6876 , G01N33/52 , G01N33/582 , G01N2333/9126
Abstract: The invention provides a novel class of cyanine dyes that are functionalized with a linker moiety that facilitates their conjugation to other species and substituent groups which increase the water-solubility, and optimize the optical properties of the dyes. Also provided are conjugates of the dyes, methods of using the dyes and their conjugates and kits including the dyes and their conjugates.
Abstract translation: 本发明提供了一类新颖的花青染料，其用接头部分官能化，所述接头部分便于它们与增加水溶性的其他物质和取代基缀合，并优化染料的光学性质。 还提供了染料的缀合物，使用染料及其缀合物的方法和包括染料及其缀合物的试剂盒。
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公开(公告)号：WO2012027625A2
公开(公告)日：2012-03-01
申请号：PCT/US2011049249
申请日：2011-08-25
Applicant: PACIFIC BIOSCIENCES CALIFORNIA , SHEN GENE , SEBO LUBOMIR , YUE STEPHEN , SUN DAVID , BROGLEY LOUIS , FEDEROV ANDREI
Inventor： SHEN GENE , SEBO LUBOMIR , YUE STEPHEN , SUN DAVID , BROGLEY LOUIS , FEDEROV ANDREI
IPC: C12N11/00 , C07D251/12 , C07D403/04 , C12Q1/25 , C12Q1/68 , G01N33/52
CPC classification number: C12Q1/6869 , C07D209/14 , C07D401/14 , C07D405/14 , C07H19/10 , C07K5/0815 , C07K5/1019 , C09B23/0025 , C09B23/0033 , C09B23/0066 , C09B23/06 , C09B23/083 , C09B23/105 , C09B23/164 , C12Q1/6844 , C12Q1/6876 , G01N33/52 , G01N33/582 , G01N2333/9126
Abstract: The invention provides a novel class of scaffold-based labeled polymerase enzyme substrates. The polymerase enzyme substrates have a multivalent core or scaffold to which is attached fluorescent dye moieties and nucleoside phosphate moities. The polymerase enzyme substrates have multiple fluorescent dye moities and/or multiple nucleoside phosphate moieties. Preferred multivalent cores comprise trifunctional six membered aromatic moities. The invention also provides for sequencing methods and kits with scaffold-based labeled polymerase enzyme substrates.
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