-
公开(公告)号：AU2012216605B2
公开(公告)日：2014-06-12
申请号：AU2012216605
申请日：2012-09-03
Applicant: PACIFIC BIOSCIENCES CALIFORNIA
Inventor： RANK DAVID R , WEGENER JEFFERY , KORLACH JONAS , ROITMAN DANIEL , XU YUE , LYLE JOHN , TURNER STEPHEN , PELUSO PAUL , OTTO GEOFF , CICERO RONALD L
Abstract: Methods of producing substrates having selected active chemical regions by employing elements of the substrates in assisting the localization of active chemical groups in desired regions of the substrate. The methods may include optical, chemical and/or mechanical processes for the deposition, removal, activation and/or deactivation of chemical groups in selected regions of the substrate to provide selective active regions of the substrate.
-
公开(公告)号：AU2009251883A1
公开(公告)日：2009-12-03
申请号：AU2009251883
申请日：2009-03-30
Applicant: PACIFIC BIOSCIENCES CALIFORNIA
Inventor： BJORNSON KEITH , EMIG ROBIN , PELUSO PAUL , HE MOLLY , CLARK SONYA , BIBILLO AREK , LEE HAROLD , JIA LEI , CHRISTIANS FRED , PARK INSIL
Abstract: Provided are compositions comprising modified recombinant polymerases that exhibit branching fractions that are less than the branching fractions of the polymerases from which they were derived, or branching fractions that are less than about 25% for a phosphate-labeled nucleotide analog. Also provided are compositions comprising modified recombinant polymerases that exhibit closed polymerase/DNA complexes with increased stability relative to the parental polymerase. Also provided are compositions comprising modified recombinant polymerases that exhibit decreased rate constants relative to the parental polymerases. Provided are methods for generating polymerases with the aforementioned phenotypes. Provided are methods of using such polymerases to make a DNA or to sequence a DNA template.
-
公开(公告)号：AU2007260707A1
公开(公告)日：2007-12-21
申请号：AU2007260707
申请日：2007-06-15
Applicant: PACIFIC BIOSCIENCES CALIFORNIA INC
Inventor： SHEN GENE , PELUSO PAUL , LYLE JOHN
IPC: C12Q1/68
Abstract: Controlled initiation of primer extension in determination of nucleic acid sequence information by incorporation of nucleotides or nucleotide analogs. Preferred aspects include photo-initiated extension through the use of photo-cleavable blocking, groups on termini of primer sequences followed by non-terminating primer extension using nucleotides or nucleotide analogs that are not extension terminators.
-
公开(公告)号：AU2006299641A1
公开(公告)日：2007-04-12
申请号：AU2006299641
申请日：2006-09-29
Applicant: PACIFIC BIOSCIENCES CALIFORNIA
Inventor： ROITMAN DANIEL BERNARDO , PELUSO PAUL , FOQUET MATHIEU
IPC: G01N33/53
-
公开(公告)号：WO2008051530A9
公开(公告)日：2008-06-26
申请号：PCT/US2007022459
申请日：2007-10-22
Applicant: PACIFIC BIOSCIENCES CALIFORNIA , RANK DAVID R , BIBILLO AREK , HANZEL DAVID K , PELUSO PAUL , WEGENER JEFF
Inventor： RANK DAVID R , BIBILLO AREK , HANZEL DAVID K , PELUSO PAUL , WEGENER JEFF
CPC classification number: C12N9/1252 , C12P19/34
Abstract: Compositions that include DNA polymerases having increased residence times for nucleotide analogues, particularly modified recombinant F29-type DNA polymerases with such increased residence times, are provided. Methods of making the polymerases and of using the polymerases in sequencing and DNA amplification are also provided. Compositions including a-thiophosphate nucleotide analogues with four or more phosphate groups are described, as are methods for determining the sequence of nucleic acid molecules using such analogues.
Abstract translation: 提供了包含具有增加的核苷酸类似物停留时间的DNA聚合酶的组合物，特别是具有这种增加的停留时间的经修饰的重组F29型DNA聚合酶。 还提供了在测序和DNA扩增中制备聚合酶和使用聚合酶的方法。 描述了包括具有四个或更多个磷酸基团的α-硫代磷酸核苷酸类似物的组合物，以及使用这种类似物测定核酸分子的序列的方法。
-
Patent Agency Ranking
公开(公告)号：WO2007076057A3
公开(公告)日：2008-06-26
申请号：PCT/US2006049122
申请日：2006-12-21
Applicant: PACIFIC BIOSCIENCES CALIFORNIA , HANZEL DAVID K , OTTO GEOFF , MURPHY DEVON , PELUSO PAUL , PHAM THANG , RANK DAVID R , MITSIS PAUL , CHRISTIANS FRED C , BIBILLO AREK , PARK INSIL , CLARK SONYA , LYLE JOHN
Inventor： HANZEL DAVID K , OTTO GEOFF , MURPHY DEVON , PELUSO PAUL , PHAM THANG , RANK DAVID R , MITSIS PAUL , CHRISTIANS FRED C , BIBILLO AREK , PARK INSIL , CLARK SONYA , LYLE JOHN
IPC: C12Q1/68
CPC classification number: C12N9/1252 , C07K2319/20 , C12P19/34 , C12Q1/6827 , C12Q1/6869 , C12Y207/07007 , Y02P20/52
Abstract: Compositions that include polymerases with features for improving entry of nucleotide analogues into active site regions and for coordinating with the nucleotide analogues in the active site region are provided. Methods of making the polymerases and of using the polymerases in sequencing and DNA replication and amplification as well as kinetic models of polymerase activity and computer-implemented methods of using the models are also provided.
Abstract translation: 提供了包含具有改善核苷酸类似物进入活性位点区域并与活性位点区域中的核苷酸类似物配位的特征的聚合酶的组合物。 还提供了制备聚合酶和在测序和DNA复制和扩增中使用聚合酶的方法以及聚合酶活性的动力学模型和使用该模型的计算机实现的方法。
-
7.发明申请GENERATION OF MODIFIED POLYMERASES FOR IMPROVED ACCURACY IN SINGLE MOLECULE SEQUENCING 审中-公开在单分子序列中改进的改进聚合物的生成
公开(公告)号：WO2011040971A2
公开(公告)日：2011-04-07
申请号：PCT/US2010002659
申请日：2010-09-30
Applicant: PACIFIC BIOSCIENCES CALIFORNIA , CLARK SONYA , BIBILLO AREK , PELUSO PAUL , CHRISTIANS FRED , HE MOLLY , PARK INSIL , LEE HAROLD , BJORNSON KEITH , JIA LEI , EMIG ROBIN
Inventor： CLARK SONYA , BIBILLO AREK , PELUSO PAUL , CHRISTIANS FRED , HE MOLLY , PARK INSIL , LEE HAROLD , BJORNSON KEITH , JIA LEI , EMIG ROBIN
CPC classification number: C12N9/1252 , C12Q1/6869 , C12Q2521/101
Abstract: Provided are compositions comprising recombinant DNA polymerases that include amino acid substitutions, insertions, deletions and/or heterologous or exogenous features that confer modified properties upon the polymerase for enhanced single molecule sequencing. Such properties can include reduced reaction rates at one or more steps of the polymerase kinetic cycle, increased closed polymerase/DNA complex stability, enhanced metal ion coordination, reduced exonuclease activity, decreased branching fractions, and the like. Also provided are nucleic acids which encode the polymerases with the aforementioned phenotypes, as well as methods of using such polymerases to make a DNA or to sequence a DNA template.
Abstract translation: 提供了包含重组DNA聚合酶的组合物，其包括氨基酸取代，插入，缺失和/或异源或外源特征，其在聚合酶上赋予改进的性质以用于增强的单分子测序。 这种性质可以包括聚合酶动力学循环的一个或多个步骤的降低的反应速率，增加的闭合聚合酶/ DNA复合物稳定性，增强的金属离子配位，减少的核酸外切酶活性，降低的支化部分等。 还提供了编码具有上述表型的聚合酶的核酸，以及使用这种聚合酶制备DNA或顺序DNA模板的方法。
-
8.发明申请PROTEIN ENGINEERING STRATEGIES TO OPTIMIZE ACTIVITY OF SURFACE ATTACHED PROTEINS 审中-公开蛋白质工程策略优化表面附着蛋白的活性
公开(公告)号：WO2007075873A3
公开(公告)日：2007-11-29
申请号：PCT/US2006048764
申请日：2006-12-21
Applicant: PACIFIC BIOSCIENCES CALIFORNIA , HANZEL DAVID , KORLACH JONAS , PELUSO PAUL , OTTO GEOFF , PHAM THANG , RANK DAVID , TURNER STEPHEN
Inventor： HANZEL DAVID , KORLACH JONAS , PELUSO PAUL , OTTO GEOFF , PHAM THANG , RANK DAVID , TURNER STEPHEN
CPC classification number: C12N9/22 , C07K2319/21 , Y10T428/2991 , Y10T428/2993 , Y10T428/2996 , Y10T428/31663 , Y10T428/31768
Abstract: Isolated and/or recombinant enzymes that include surface binding domains, surfaces with active enzymes bound to them and methods of coupling enzymes to surfaces are provided. Enzymes can include large and/or multiple surface coupling domains for surface coupling.
Abstract translation: 提供了包括表面结合结构域，具有与其结合的活性酶的表面和将酶偶联到表面的方法的分离和/或重组酶。 酶可以包括用于表面偶联的大和/或多个表面偶联域。
-
公开(公告)号：CA2633476C
公开(公告)日：2015-04-21
申请号：CA2633476
申请日：2006-12-21
Applicant: PACIFIC BIOSCIENCES CALIFORNIA
Inventor： HANZEL DAVID , KORLACH JONAS , PELUSO PAUL , OTTO GEOFFREY , PHAM THANG , RANK DAVID , TURNER STEPHEN
Abstract: Active surface coupled polymerases, surfaces that include such polymerases, and methods of making and using surface-attached polymerases are provided.
-
公开(公告)号：AU2012216605A1
公开(公告)日：2012-09-20
申请号：AU2012216605
申请日：2012-09-03
Applicant: PACIFIC BIOSCIENCES CALIFORNIA
Inventor： RANK DAVID R , WEGENER JEFFERY , KORLACH JONAS , ROITMAN DANIEL , XU YUE , LYLE JOHN , TURNER STEPHEN , PELUSO PAUL , OTTO GEOFF
Abstract: Methods of producing substrates having selected active chemical regions by employing elements of the substrates in assisting the localization of active chemical groups in desired regions of the substrate. The methods may include optical, chemical and/or mechanical processes for the deposition, removal, activation and/or deactivation of chemical groups in selected regions of the substrate to provide selective active regions of the substrate.
-
-
-
-
-
-
-
-
-
Search Results
57
records
(took 0.024 seconds)
