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公开(公告)号：AU2006330947B2
公开(公告)日：2012-04-12
申请号：AU2006330947
申请日：2006-12-21
Applicant: PACIFIC BIOSCIENCES CALIFORNIA INC
Inventor： BIBILLO AREK , MITSIS PAUL , PHAM THANG , CHRISTIANS FRED C , MURPHY DEVON , PARK INSIL , HANZEL DAVID K , LYLE JOHN , RANK DAVID R , CLARK SONYA , PELUSO PAUL , OTTO GEOFF
IPC: C12Q1/68
Abstract: Compositions that include polymerases with features for improving entry of nucleotide analogues into active site regions and for coordinating with the nucleotide analogues in the active site region are provided. Methods of making the polymerases and of using the polymerases in sequencing and DNA replication and amplification as well as kinetic models of polymerase activity and computer-implemented methods of using the models are also provided.
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公开(公告)号：AU2006297104A1
公开(公告)日：2007-04-12
申请号：AU2006297104
申请日：2006-09-28
Applicant: PACIFIC BIOSCIENCES CALIFORNIA INC
Inventor： BIBILLO AREK , WEGENER JEFFEY , XU YUE
Abstract: Labeled nucleotide analogs used in place of naturally occurring nucleoside triphosphates or other analogs in template directed nucleic acid synthesis reactions and other nucleic acid reactions, and various analyses based thereon, including DNA sequencing, single base identification, hybridization assays and others.
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公开(公告)号：AU2007309504B2
公开(公告)日：2012-09-13
申请号：AU2007309504
申请日：2007-10-22
Applicant: PACIFIC BIOSCIENCES CALIFORNIA INC
Inventor： PELUSO PAUL , RANK DAVID R , BIBILLO AREK , WEGENER JEFF , HANZEL DAVID K
Abstract: Compositions that include DNA polymerases having increased residence times for nucleotide analogues, particularly modified recombinant Φ29-type DNA polymerases with such increased residence times, are provided. Methods of making the polymerases and of using the polymerases in sequencing and DNA amplification are also provided. Compositions including α-thiophosphate nucleotide analogues with four or more phosphate groups are described, as are methods for determining the sequence of nucleic acid molecules using such analogues.
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Patent Agency Ranking4.发明专利
公开(公告)号：ES2614078T3
公开(公告)日：2017-05-29
申请号：ES15168606
申请日：2009-03-30
Applicant: PACIFIC BIOSCIENCES CALIFORNIA INC
Inventor： CLARK SONYA , BIBILLO AREK , PELUSO PAUL , CHRISTIANS FRED , HE MOLLY , PARK INSIL , LEE HAROLD , BJORNSON KEITH , JIA LEI , EMIG ROBIN
Abstract: Una composición que comprende una ADN polimerasa recombinante, ADN polimerasa recombinante que comprende una secuencia de aminoácidos que es al menos el 80 % idéntica a la SEQ ID NO: 1, ADN polimerasa recombinante que comprende un resto de ácido glutámico, un resto de ácido aspártico, un resto de histidina, un resto de lisina, un resto de arginina o un resto de tirosina en la posición A484, en la que la identificación de las posiciones es con respecto a la SEQ ID NO: 1, y ADN polimerasa recombinante que muestra actividad polimerasa.
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公开(公告)号：AU2009251883B2
公开(公告)日：2014-09-11
申请号：AU2009251883
申请日：2009-03-30
Applicant: PACIFIC BIOSCIENCES CALIFORNIA INC
Inventor： JIA LEI , BJORNSON KEITH , PARK INSIL , CLARK SONYA , CHRISTIANS FRED , EMIG ROBIN , HE MOLLY , PELUSO PAUL , LEE HAROLD , BIBILLO AREK
Abstract: Provided are compositions comprising modified recombinant polymerases that exhibit branching fractions that are less than the branching fractions of the polymerases from which they were derived, or branching fractions that are less than about 25% for a phosphate-labeled nucleotide analog. Also provided are compositions comprising modified recombinant polymerases that exhibit closed polymerase/DNA complexes with increased stability relative to the parental polymerases. Also provided are compositions comprising modified recombinant polymerases that exhibit decreased rate constants relative to the parental polymerases. Provided are methods for generating polymerases with the aforementioned phenotypes. Provided are methods of using such polymerases to make a DNA or to sequence a DNA template.
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公开(公告)号：CA2720046C
公开(公告)日：2018-07-24
申请号：CA2720046
申请日：2009-03-30
Applicant: PACIFIC BIOSCIENCES CALIFORNIA INC
Inventor： CLARK SONYA , BIBILLO AREK , PELUSO PAUL , CHRISTIANS FRED , HE MOLLY , PARK INSIL , LEE HAROLD , BJORNSON KEITH , JIA LEI , EMIG ROBIN
Abstract: Provided are compositions comprising modified recombinant polymerases that exhibit branching fractions that are less than the branching fractions of the polymerases from which they were derived, or branching fractions that are less than about 25% for a phosphate-labeled nucleotide analog. Also provided are compositions comprising modified recombinant polymerases that exhibit closed polymerase/DNA complexes with increased stability relative to the parental polymerases. Also provided are compositions comprising modified recombinant polymerases that exhibit decreased rate constants relative to the parental polymerases. Provided are methods for generating polymerases with the aforementioned phenotypes. Provided are methods of using such polymerases to make a DNA or to sequence a DNA template.
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公开(公告)号：ES2648313T3
公开(公告)日：2017-12-29
申请号：ES16157104
申请日：2006-12-21
Applicant: PACIFIC BIOSCIENCES CALIFORNIA INC
Inventor： HANZEL DAVID , OTTO GEOFF , MURPHY DEVON , PELUSO PAUL , PHAM THANG , RANK DAVID , MITSIS PAUL , CHRISTIANS FRED , BIBILLO AREK , PARK INSIL , CLARK SONYA , LYLE JOHN
IPC: C12Q1/68
Abstract: Una composición que comprende: una ADN polimerasa recombinante, comprendiendo dicha polimerasa recombinante una secuencia de aminoácidos que es al menos un 70 % idéntica a la SEQ ID NO:1 y comprende una sustitución de aminoácido en un sitio correspondiente a K512 respecto a la SEQ ID NO:1; y un análogo nucleotídico que está marcado en un grupo fosfato, en el que el resto de base del análogo se selecciona del grupo que consiste en adenina, timina, guanina y citidina.
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公开(公告)号：AU2009215193A1
公开(公告)日：2009-08-20
申请号：AU2009215193
申请日：2009-02-12
Applicant: PACIFIC BIOSCIENCES CALIFORNIA INC
Inventor： SEBO LUBOMIR , BIBILLO AREK , BJORNSON KEITH
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