公开(公告)号：CA2633476A1

公开(公告)日：2007-07-05

申请号：CA2633476

申请日：2006-12-21

Applicant: PACIFIC BIOSCIENCES CALIFORNIA INC

Inventor： KORLACH JONAS ,  HANZEL DAVID ,  RANK DAVID ,  PHAM THANG ,  TURNER STEPHEN ,  PELUSO PAUL ,  OTTO GEOFFREY

IPC: C12Q1/68 ,  C12M3/00

Abstract: Active surface coupled polymerases, surfaces that include such polymerases, and methods of making and using surface-attached polymerases are provided.

公开(公告)号：AU2006331677A1

公开(公告)日：2007-07-05

申请号：AU2006331677

申请日：2006-12-21

Applicant: PACIFIC BIOSCIENCES CALIFORNIA INC

Inventor： RANK DAVID ,  KORLACH JONAS ,  HANZEL DAVID ,  PHAM THANG ,  OTTO GEOFF ,  TURNER STEPHEN ,  PELUSO PAUL

IPC: C12N11/00 ,  C12N11/16 ,  G01N33/53

公开(公告)号：AU2006330947B2

公开(公告)日：2012-04-12

申请号：AU2006330947

申请日：2006-12-21

Applicant: PACIFIC BIOSCIENCES CALIFORNIA INC

Inventor： BIBILLO AREK ,  MITSIS PAUL ,  PHAM THANG ,  CHRISTIANS FRED C ,  MURPHY DEVON ,  PARK INSIL ,  HANZEL DAVID K ,  LYLE JOHN ,  RANK DAVID R ,  CLARK SONYA ,  PELUSO PAUL ,  OTTO GEOFF

IPC: C12Q1/68

Abstract: Compositions that include polymerases with features for improving entry of nucleotide analogues into active site regions and for coordinating with the nucleotide analogues in the active site region are provided. Methods of making the polymerases and of using the polymerases in sequencing and DNA replication and amplification as well as kinetic models of polymerase activity and computer-implemented methods of using the models are also provided.

公开(公告)号：ES2648313T3

公开(公告)日：2017-12-29

申请号：ES16157104

申请日：2006-12-21

Applicant: PACIFIC BIOSCIENCES CALIFORNIA INC

Inventor： HANZEL DAVID ,  OTTO GEOFF ,  MURPHY DEVON ,  PELUSO PAUL ,  PHAM THANG ,  RANK DAVID ,  MITSIS PAUL ,  CHRISTIANS FRED ,  BIBILLO AREK ,  PARK INSIL ,  CLARK SONYA ,  LYLE JOHN

IPC: C12Q1/68

Abstract: Una composición que comprende: una ADN polimerasa recombinante, comprendiendo dicha polimerasa recombinante una secuencia de aminoácidos que es al menos un 70 % idéntica a la SEQ ID NO:1 y comprende una sustitución de aminoácido en un sitio correspondiente a K512 respecto a la SEQ ID NO:1; y un análogo nucleotídico que está marcado en un grupo fosfato, en el que el resto de base del análogo se selecciona del grupo que consiste en adenina, timina, guanina y citidina.

公开(公告)号：AU2006331512B2

公开(公告)日：2012-02-23

申请号：AU2006331512

申请日：2006-12-21

Applicant: PACIFIC BIOSCIENCES CALIFORNIA INC

Inventor： KORLACH JONAS ,  OTTO GEOFFREY ,  HANZEL DAVID ,  PELUSO PAUL ,  TURNER STEPHEN ,  RANK DAVID ,  PHAM THANG

IPC: C12Q1/68 ,  C12N11/14

Abstract: Active surface coupled polymerases, surfaces that include such polymerases, and methods of making and using surface-attached polymerases are provided.