公开(公告)号：CA2746632C

公开(公告)日：2020-06-30

申请号：CA2746632

申请日：2009-12-10

Applicant: PACIFIC BIOSCIENCES CALIFORNIA INC

Inventor： FLUSBERG BENJAMIN ,  TURNER STEPHEN ,  LEE JESSICA ,  JIA LEI ,  KORLACH JONAS ,  SORENSON JON ,  WEBSTER DALE ,  LYLE JOHN ,  TRAVERS KEVIN ,  HANES JEREMIAH ,  PUGLISI JOSEPH

IPC: C12Q1/68 ,  C12Q1/6809 ,  C12Q1/6869

Abstract: Methods, compositions, and systems are provided for characterization of modified nucleic acids. In certain preferred embodiments, single molecule sequencing methods are provided for identification of modified nucleotides within nucleic acid sequences. Modifications detectable by the methods provided herein include chemically modified bases, enzymatically modified bases, abasic sites, non-natural bases, secondary structures, and agents bound to a template nucleic acid.

公开(公告)号：CA2720046C

公开(公告)日：2018-07-24

申请号：CA2720046

申请日：2009-03-30

Applicant: PACIFIC BIOSCIENCES CALIFORNIA INC

Inventor： CLARK SONYA ,  BIBILLO AREK ,  PELUSO PAUL ,  CHRISTIANS FRED ,  HE MOLLY ,  PARK INSIL ,  LEE HAROLD ,  BJORNSON KEITH ,  JIA LEI ,  EMIG ROBIN

IPC: C12N9/12 ,  C12N15/10 ,  C12N15/54

Abstract: Provided are compositions comprising modified recombinant polymerases that exhibit branching fractions that are less than the branching fractions of the polymerases from which they were derived, or branching fractions that are less than about 25% for a phosphate-labeled nucleotide analog. Also provided are compositions comprising modified recombinant polymerases that exhibit closed polymerase/DNA complexes with increased stability relative to the parental polymerases. Also provided are compositions comprising modified recombinant polymerases that exhibit decreased rate constants relative to the parental polymerases. Provided are methods for generating polymerases with the aforementioned phenotypes. Provided are methods of using such polymerases to make a DNA or to sequence a DNA template.

公开(公告)号：AU2011326026A1

公开(公告)日：2013-05-02

申请号：AU2011326026

申请日：2011-11-11

Applicant: PACIFIC BIOSCIENCES CALIFORNIA INC

Inventor： FLUSBERG BENJAMIN ,  KORLACH JONAS ,  WEGENER JEFFREY ,  CLARK TYSON A ,  VILFAN IGOR ,  KISLYUK ANDREY ,  TURNER STEPHEN ,  SORENSON JON ,  TRAVERS KEVIN ,  HEINER CHERYL ,  TOMANEY AUSTIN B ,  MARKS PATRICK ,  LEE JESSICA ,  JIA LEI ,  WEBSTER DALE ,  LYLE JOHN ,  HANES JEREMIAH ,  PUGLISI JOSEPH

IPC: C12Q1/68 ,  C12N15/11 ,  C12Q1/25 ,  C12Q1/34 ,  C12Q1/48

Abstract: Methods, compositions, and systems are provided for characterization of modified nucleic acids. In certain preferred embodiments, single molecule sequencing methods are provided for identification of modified nucleotides within nucleic acid sequences. Modifications detectable by the methods provided herein include chemically modified bases, enzymatically modified bases, abasic sites, non-natural bases, secondary structures, and agents bound to a template nucleic acid.

公开(公告)号：ES2614078T3

公开(公告)日：2017-05-29

申请号：ES15168606

申请日：2009-03-30

Applicant: PACIFIC BIOSCIENCES CALIFORNIA INC

Inventor： CLARK SONYA ,  BIBILLO AREK ,  PELUSO PAUL ,  CHRISTIANS FRED ,  HE MOLLY ,  PARK INSIL ,  LEE HAROLD ,  BJORNSON KEITH ,  JIA LEI ,  EMIG ROBIN

IPC: C12Q1/68 ,  C12N9/12 ,  C12Q1/48

Abstract: Una composición que comprende una ADN polimerasa recombinante, ADN polimerasa recombinante que comprende una secuencia de aminoácidos que es al menos el 80 % idéntica a la SEQ ID NO: 1, ADN polimerasa recombinante que comprende un resto de ácido glutámico, un resto de ácido aspártico, un resto de histidina, un resto de lisina, un resto de arginina o un resto de tirosina en la posición A484, en la que la identificación de las posiciones es con respecto a la SEQ ID NO: 1, y ADN polimerasa recombinante que muestra actividad polimerasa.

公开(公告)号：AU2009325069B2

公开(公告)日：2015-03-19

申请号：AU2009325069

申请日：2009-12-10

Applicant: PACIFIC BIOSCIENCES CALIFORNIA INC

Inventor： FLUSBERG BENJAMIN ,  TURNER STEPHEN ,  LEE JESSICA ,  JIA LEI ,  KORLACH JONAS ,  SORENSON JON ,  WEBSTER DALE ,  LYLE JOHN ,  TRAVERS KEVIN ,  HANES JEREMIAH

IPC: C12Q1/68 ,  C12N15/10

Abstract: Methods, compositions, and systems are provided for characterization of modified nucleic acids. In certain preferred embodiments, single molecule sequencing methods are provided for identification of modified nucleotides within nucleic acid sequences. Modifications detectable by the methods provided herein include chemically modified bases, enzymatically modified bases, abasic sites, non-natural bases, secondary structures, and agents bound to a template nucleic acid.

公开(公告)号：AU2009251883B2

公开(公告)日：2014-09-11

申请号：AU2009251883

申请日：2009-03-30

Applicant: PACIFIC BIOSCIENCES CALIFORNIA INC

Inventor： JIA LEI ,  BJORNSON KEITH ,  PARK INSIL ,  CLARK SONYA ,  CHRISTIANS FRED ,  EMIG ROBIN ,  HE MOLLY ,  PELUSO PAUL ,  LEE HAROLD ,  BIBILLO AREK

IPC: C12N9/12 ,  C12N15/10 ,  C12N15/54

Abstract: Provided are compositions comprising modified recombinant polymerases that exhibit branching fractions that are less than the branching fractions of the polymerases from which they were derived, or branching fractions that are less than about 25% for a phosphate-labeled nucleotide analog. Also provided are compositions comprising modified recombinant polymerases that exhibit closed polymerase/DNA complexes with increased stability relative to the parental polymerases. Also provided are compositions comprising modified recombinant polymerases that exhibit decreased rate constants relative to the parental polymerases. Provided are methods for generating polymerases with the aforementioned phenotypes. Provided are methods of using such polymerases to make a DNA or to sequence a DNA template.