公开(公告)号：AU2006330947B2

公开(公告)日：2012-04-12

申请号：AU2006330947

申请日：2006-12-21

Applicant: PACIFIC BIOSCIENCES CALIFORNIA INC

Inventor： BIBILLO AREK ,  MITSIS PAUL ,  PHAM THANG ,  CHRISTIANS FRED C ,  MURPHY DEVON ,  PARK INSIL ,  HANZEL DAVID K ,  LYLE JOHN ,  RANK DAVID R ,  CLARK SONYA ,  PELUSO PAUL ,  OTTO GEOFF

IPC: C12Q1/68

Abstract: Compositions that include polymerases with features for improving entry of nucleotide analogues into active site regions and for coordinating with the nucleotide analogues in the active site region are provided. Methods of making the polymerases and of using the polymerases in sequencing and DNA replication and amplification as well as kinetic models of polymerase activity and computer-implemented methods of using the models are also provided.

公开(公告)号：ES2614078T3

公开(公告)日：2017-05-29

申请号：ES15168606

申请日：2009-03-30

Applicant: PACIFIC BIOSCIENCES CALIFORNIA INC

Inventor： CLARK SONYA ,  BIBILLO AREK ,  PELUSO PAUL ,  CHRISTIANS FRED ,  HE MOLLY ,  PARK INSIL ,  LEE HAROLD ,  BJORNSON KEITH ,  JIA LEI ,  EMIG ROBIN

IPC: C12Q1/68 ,  C12N9/12 ,  C12Q1/48

Abstract: Una composición que comprende una ADN polimerasa recombinante, ADN polimerasa recombinante que comprende una secuencia de aminoácidos que es al menos el 80 % idéntica a la SEQ ID NO: 1, ADN polimerasa recombinante que comprende un resto de ácido glutámico, un resto de ácido aspártico, un resto de histidina, un resto de lisina, un resto de arginina o un resto de tirosina en la posición A484, en la que la identificación de las posiciones es con respecto a la SEQ ID NO: 1, y ADN polimerasa recombinante que muestra actividad polimerasa.

公开(公告)号：AU2009251883B2

公开(公告)日：2014-09-11

申请号：AU2009251883

申请日：2009-03-30

Applicant: PACIFIC BIOSCIENCES CALIFORNIA INC

Inventor： JIA LEI ,  BJORNSON KEITH ,  PARK INSIL ,  CLARK SONYA ,  CHRISTIANS FRED ,  EMIG ROBIN ,  HE MOLLY ,  PELUSO PAUL ,  LEE HAROLD ,  BIBILLO AREK

IPC: C12N9/12 ,  C12N15/10 ,  C12N15/54

Abstract: Provided are compositions comprising modified recombinant polymerases that exhibit branching fractions that are less than the branching fractions of the polymerases from which they were derived, or branching fractions that are less than about 25% for a phosphate-labeled nucleotide analog. Also provided are compositions comprising modified recombinant polymerases that exhibit closed polymerase/DNA complexes with increased stability relative to the parental polymerases. Also provided are compositions comprising modified recombinant polymerases that exhibit decreased rate constants relative to the parental polymerases. Provided are methods for generating polymerases with the aforementioned phenotypes. Provided are methods of using such polymerases to make a DNA or to sequence a DNA template.

公开(公告)号：CA2720046C

公开(公告)日：2018-07-24

申请号：CA2720046

申请日：2009-03-30

Applicant: PACIFIC BIOSCIENCES CALIFORNIA INC

Inventor： CLARK SONYA ,  BIBILLO AREK ,  PELUSO PAUL ,  CHRISTIANS FRED ,  HE MOLLY ,  PARK INSIL ,  LEE HAROLD ,  BJORNSON KEITH ,  JIA LEI ,  EMIG ROBIN

IPC: C12N9/12 ,  C12N15/10 ,  C12N15/54

Abstract: Provided are compositions comprising modified recombinant polymerases that exhibit branching fractions that are less than the branching fractions of the polymerases from which they were derived, or branching fractions that are less than about 25% for a phosphate-labeled nucleotide analog. Also provided are compositions comprising modified recombinant polymerases that exhibit closed polymerase/DNA complexes with increased stability relative to the parental polymerases. Also provided are compositions comprising modified recombinant polymerases that exhibit decreased rate constants relative to the parental polymerases. Provided are methods for generating polymerases with the aforementioned phenotypes. Provided are methods of using such polymerases to make a DNA or to sequence a DNA template.

公开(公告)号：ES2648313T3

公开(公告)日：2017-12-29

申请号：ES16157104

申请日：2006-12-21

Applicant: PACIFIC BIOSCIENCES CALIFORNIA INC

Inventor： HANZEL DAVID ,  OTTO GEOFF ,  MURPHY DEVON ,  PELUSO PAUL ,  PHAM THANG ,  RANK DAVID ,  MITSIS PAUL ,  CHRISTIANS FRED ,  BIBILLO AREK ,  PARK INSIL ,  CLARK SONYA ,  LYLE JOHN

IPC: C12Q1/68

Abstract: Una composición que comprende: una ADN polimerasa recombinante, comprendiendo dicha polimerasa recombinante una secuencia de aminoácidos que es al menos un 70 % idéntica a la SEQ ID NO:1 y comprende una sustitución de aminoácido en un sitio correspondiente a K512 respecto a la SEQ ID NO:1; y un análogo nucleotídico que está marcado en un grupo fosfato, en el que el resto de base del análogo se selecciona del grupo que consiste en adenina, timina, guanina y citidina.