公开(公告)号：AU2021222009A1

公开(公告)日：2022-09-15

申请号：AU2021222009

申请日：2021-02-18

Applicant: PACIFIC BIOSCIENCES CALIFORNIA INC

Inventor： KURITSYN ALEXEY ,  MCDONALD MARK ,  RULISON AARON ,  GROT ANNETTE

IPC: G02B6/12 ,  C12Q1/6874 ,  G01N21/00 ,  G01N21/17 ,  G01N21/63 ,  G01N21/78

Abstract: Provided herein are highly multiplexed optical analytical systems for improved nucleic acid sequencing. The systems include a plurality of highly multiplexed optical chips, at least one optical source, and a plurality of optical delivery devices for illuminating an array of nanoscale rection regions on each of the optical chips. In use, the reaction regions contain fluorescent nucleic acid sequencing reagents and are arranged to report nucleic acid sequence information to optical detectors associated with the multiplexed optical chips in real time. The systems enable a massive increase in the scale of nucleic acid sequencing reactions capable of being performed within a single instrument without a corresponding increase in size, complexity, or cost of the instrument.

公开(公告)号：AU2016276980B2

公开(公告)日：2021-09-23

申请号：AU2016276980

申请日：2016-06-11

Applicant: PACIFIC BIOSCIENCES CALIFORNIA INC

Inventor： WANG SHANG ,  FOQUET MATHIEU ,  LUNDQUIST PAUL ,  RULISON AARON ,  MCDONALD MARK ,  HERRMANN ARIEL

IPC: G02B6/26 ,  G02B6/34

Abstract: Integrated target waveguide devices and optical analytical systems comprising such devices are provided. The target devices include an optical coupler that is optically coupled to an integrated waveguide and that is configured to receive optical input from an optical source through free space, particularly through a low numerical aperture interface. The devices and systems are useful in the analysis of highly multiplexed optical reactions in large numbers at high densities, including biochemical reactions, such as nucleic acid sequencing reactions. The devices provide for the efficient and reliable coupling of optical excitation energy from an optical source to the optical reactions. Optical signals emitted from the reactions can thus be measured with high sensitivity and discrimination. The devices and systems are well suited for miniaturization and high throughput.

公开(公告)号：CA2989344A1

公开(公告)日：2016-12-15

申请号：CA2989344

申请日：2016-06-11

Applicant: PACIFIC BIOSCIENCES CALIFORNIA INC

Inventor： WANG SHANG ,  FOQUET MATHIEU ,  LUNDQUIST PAUL ,  RULISON AARON ,  MCDONALD MARK ,  HERRMANN ARIEL

IPC: G02B6/26 ,  G02B6/34

Abstract: Integrated target waveguide devices and optical analytical systems comprising such devices are provided. The target devices include an optical coupler that is optically coupled to an integrated waveguide and that is configured to receive optical input from an optical source through free space, particularly through a low numerical aperture interface. The devices and systems are useful in the analysis of highly multiplexed optical reactions in large numbers at high densities, including biochemical reactions, such as nucleic acid sequencing reactions. The devices provide for the efficient and reliable coupling of optical excitation energy from an optical source to the optical reactions. Optical signals emitted from the reactions can thus be measured with high sensitivity and discrimination. The devices and systems are well suited for miniaturization and high throughput.

公开(公告)号：AU2016276980A1

公开(公告)日：2018-01-25

申请号：AU2016276980

申请日：2016-06-11

Applicant: PACIFIC BIOSCIENCES CALIFORNIA INC

Inventor： WANG SHANG ,  FOQUET MATHIEU ,  LUNDQUIST PAUL ,  RULISON AARON ,  MCDONALD MARK ,  HERRMANN ARIEL

IPC: G02B6/26 ,  G02B6/34

Abstract: Integrated target waveguide devices and optical analytical systems comprising such devices are provided. The target devices include an optical coupler that is optically coupled to an integrated waveguide and that is configured to receive optical input from an optical source through free space, particularly through a low numerical aperture interface. The devices and systems are useful in the analysis of highly multiplexed optical reactions in large numbers at high densities, including biochemical reactions, such as nucleic acid sequencing reactions. The devices provide for the efficient and reliable coupling of optical excitation energy from an optical source to the optical reactions. Optical signals emitted from the reactions can thus be measured with high sensitivity and discrimination. The devices and systems are well suited for miniaturization and high throughput.

公开(公告)号：CA2738626C

公开(公告)日：2017-08-08

申请号：CA2738626

申请日：2009-09-25

Applicant: PACIFIC BIOSCIENCES CALIFORNIA INC

Inventor： ZACCARIN DENIS ,  LUNDQUIST PAUL ,  ZHAO PEIQIAN ,  ZHONG FRANK ,  TURNER STEPHEN ,  HUANG YANQIAO ,  MONADGEMI PEZHMAN ,  SAXENA RAVI ,  GROT ANNETTE ,  RULISON AARON

IPC: G02B6/12 ,  G01N21/00

Abstract: Apparatus, systems and methods for use in analyzing discrete reactions at ultra high multiplex with reduced optical noise, and increased system flexibility. Apparatus include substrates having integrated optical components that increase multiplex capability by one or more of increasing density of reaction regions, improving transmission of light to or collection of light from discrete reactions regions. Integrated optical components include reflective optical elements which re-direct illumination light and light emitted from the discrete regions to more efficiently collect emitted light. Particularly preferred applications include single molecule reaction analysis, such as polymerase mediated template dependent nucleic acid synthesis and sequence determination.

公开(公告)号：AU2009300369B2

公开(公告)日：2014-08-14

申请号：AU2009300369

申请日：2009-09-25

Applicant: PACIFIC BIOSCIENCES CALIFORNIA INC

Inventor： ZACCARIN DENIS ,  LUNDQUIST PAUL ,  ZHAO PEIQIAN ,  ZHONG FRANK ,  TURNER STEPHEN ,  HUANG YANQIAO ,  MONADGEMI PEZHMAN ,  SAXENA RAVI ,  GROT ANNETTE ,  RULISON AARON

IPC: G01N21/00 ,  G02B6/12

Abstract: Apparatus, systems and methods for use in analyzing discrete reactions at ultra high multiplex with reduced optical noise, and increased system flexibility. Apparatus include substrates having integrated optical components that increase multiplex capability by one or more of increasing density of reaction regions, improving transmission of light to or collection of light from discrete reactions regions. Integrated optical components include reflective optical elements which re-direct illumination light and light emitted from the discrete regions to more efficiently collect emitted light. Particularly preferred applications include single molecule reaction analysis, such as polymerase mediated template dependent nucleic acid synthesis and sequence determination.