公开(公告)号：US20220389517A1

公开(公告)日：2022-12-08

申请号：US17815082

申请日：2022-07-26

Applicant: SHIZUOKA PREFECTURE ,  SHIZUOKA PREFECTURAL UNIVERSITY CORPORATION

Inventor： Ken YAMAGUCHI ,  Masatoshi KUSUHARA ,  Masakuni SERIZAWA ,  Tohru MOCHIZUKI ,  Keiichi OHSHIMA ,  Keiichi HATAKEYAMA ,  Kenichi URAKAMI ,  Shumpei OHNAMI ,  Yasuto AKIYAMA ,  Kouji MARUYAMA ,  Kengo INOUE ,  Yuji SHIMODA ,  Takeshi NAGASHIMA ,  Yoshinobu ISHIKAWA

IPC: C12Q1/6886 ,  G01N33/574

Abstract: An object of the present invention is to provide a method for predicting a risk of developing cancer. DNA samples were prepared from blood and cancer tissues of 2480 cancer patients and analyzed for the nucleotide sequences of exon regions using NGS. As a result, among the cancer patients, 7 patients were confirmed to have D49H mutation or A159D mutation which is a germ cell mutation.

公开(公告)号：US20200255901A1

公开(公告)日：2020-08-13

申请号：US16322895

申请日：2017-08-03

Applicant: Shizuoka Prefecture ,  Shizuoka Prefectural University Corporation

Inventor： Ken YAMAGUCHI ,  Masatoshi KUSUHARA ,  Masakuni SERIZAWA ,  Tohru MOCHIZUKI ,  Keiichi OHSHIMA ,  Keiichi HATAKEYAMA ,  Kenichi URAKAMI ,  Shumpei OHNAMI ,  Yasuto AKIYAMA ,  Kouji MARUYAMA ,  Kengo INOUE ,  Yuji SHIMODA ,  Takeshi NAGASHIMA ,  Yoshinobu ISHIKAWA

IPC: C12Q1/6886 ,  G01N33/574

Abstract: An object of the present invention is to provide a method for predicting a risk of developing cancer. DNA samples were prepared from blood and cancer tissues of 2480 cancer patients and analyzed for the nucleotide sequences of exon regions using NGS. As a result, among the cancer patients, 7 patients were confirmed to have D49H mutation or A159D mutation which is a germ cell mutation.

公开(公告)号：US20240376549A1

公开(公告)日：2024-11-14

申请号：US18685363

申请日：2022-08-23

Applicant: SHIZUOKA PREFECTURE ,  SRL & SHIZUOKA CANCER CENTER COLLABORATIVE LABORATORIES INC.

Inventor： Keiichi HATAKEYAMA ,  Takashi SUGINO ,  Koji MURAMATSU ,  Keiichi OHSHIMA ,  Hirotsugu KENMOTSU ,  Kenichi URAKAMI ,  Yuji SHIMODA ,  Takeshi NAGASHIMA ,  Yuichi KAWANISHI ,  Ryutaro FUKUMURA

IPC: C12Q1/6886 ,  C12Q1/6806 ,  C12Q1/6874

Abstract: Provided are a method for detecting a gene mutation using an FFPE tissue section containing tumor cells regardless of the percentage of tumor cells, the method being capable of increasing the number of detectable gene mutations and mutant allele frequency, and a method capable of differentiating, even in the absence of blood samples, a somatic cell mutation from a germ cell line mutation. A method for detecting a gene mutation according to the present invention comprises: a dissociation step for dissociating a single cell population from a formalin-fixed paraffin-embedded tissue section containing tumor cells; a separation step for obtaining a tumor fraction containing the tumor cells from the single cell population; a collection step for collecting a nucleic acid molecule from the tumor fraction; and a sequencing step for subjecting the nucleic acid molecule to sequencing.