公开(公告)号：CA2537209A1

公开(公告)日：2005-03-31

申请号：CA2537209

申请日：2004-09-06

Applicant: TORAY INDUSTRIES

Inventor： SUGAYA HIROYUKI ,  YAMADA SATOKO ,  UTSUMI JUN ,  FUJITA YOSHIJI ,  WADA SHIGEHISA

IPC: C07K1/14 ,  B01D63/02 ,  B01D69/02 ,  C12M1/00 ,  C12P1/00 ,  G01N1/40

Abstract: A method of preparing a solution comprising combining two steps of (1) a ste p of adsorbing a protein having a molecular weight higher than that of albumin , (2) a step of fractionating a protein having a molecular weight higher than that of albumin, and (3) a step of concentrating a protein; a method of preparing a solution comprising separating by the use of a membrane separati on unit exhibiting a permeation ratio of a protein having a molecular weight le ss than 15 thousands to a protein having a molecular weight of 60 thousands or more of 50 or more; and a method of preparing a solution which comprises feeding a raw biological component solution and then a liquid for dilution t o a separating membrane module, and allowing a part of the resultant solution to permeate through a separation membrane while circulating the solution. The method can be advantageously employed for preparing a biological component- containing solution which is suitable for the clinical proteomics using mass analysis, electrophoresis, liquid chromatography, and the like.

公开(公告)号：AT540050T

公开(公告)日：2012-01-15

申请号：AT04787627

申请日：2004-09-06

Applicant: TORAY INDUSTRIES

Inventor： WADA SHIGEHISA ,  UTSUMI JUN ,  FUJITA YOSHIJI ,  SUGAYA HIROYUKI ,  YAMADA SATOKO

IPC: C07K1/22 ,  B01D63/02 ,  B01D69/02 ,  C07K1/14 ,  C07K1/34 ,  C07K1/36 ,  C12M1/00 ,  C12P1/00 ,  G01N1/40 ,  G01N33/68

Abstract: The invention provides a method of preparing a biological components-containing solution suitable for clinical proteome analysis by mass spectrometry, electrophoresis, liquid chromatography or the like and an apparatus for the method. The method of the invention is a method for combining two steps among steps of (1) adsorbing proteins having a molecular weight equal to or higher than that of albumin; (2) fractionating proteins having a molecular weight equal to or higher than that of albumin; and (3) concentrating proteins: or a method for preparing the solution by separation using a membrane separation unit having a comparative permeation ratio of 50 or higher for proteins with a molecular weight less than 15, 000 and proteins with a molecular weight of 60, 000 or higher; or a method for preparing the solution by introducing a biological components-containing raw solution into a separation membrane module and successively passing a diluting solution for circulating the solution and passing a portion of the solution through a separation membrane.

公开(公告)号：ES2380538T3

公开(公告)日：2012-05-16

申请号：ES04787627

申请日：2004-09-06

Applicant: TORAY INDUSTRIES

Inventor： WADA SHIGEHISA ,  UTSUMI JUN ,  FUJITA YOSHIJI ,  SUGAYA HIROYUKI ,  YAMADA SATOKO

IPC: C07K1/22 ,  B01D63/02 ,  B01D69/02 ,  C07K1/14 ,  C07K1/34 ,  C07K1/36 ,  C12M1/00 ,  C12P1/00 ,  G01N1/40 ,  G01N33/68

Abstract: Un procedimiento de preparación de una solución para análisis proteómico que tiene una composición cambiada de componentes biológicos en la que la proporción de concentración de albúmina en las proteínas totales es menor de 0,3 y la proporción de concentración de β2-microglobulina en las proteínas totales es al menos 10 veces más alta que la proporción de concentración en la solución de partida que contiene componentes biológicos, comprendiendo el procedimiento someter la solución que contiene componentes biológicos a tratamiento en al menos dos etapas; en el que las dos etapas se seleccionan entre (1) una etapa de adsorción de una parte o todas las proteínas que tienen un peso molecular igual o superior al de la albúmina; (2) una etapa de retiro de una parte o todas las proteínas que tienen un peso molecular igual o superior al de la albúmina mediante fraccionamiento con un tamiz molecular; y (3) una etapa de concentración de proteínas, en donde se emplea en la etapa (1) o la etapa (2) una membrana de separación de fibra hueca de polisulfona que contiene canales de flujo que tienen una estructura asimétrica que se deriva de polisulfona y poli(vinilpirrolidona) y tiene una proporción de permeación comparativa de β2-microglobulina con respecto a albúmina, definida como coeficiente de tamizado de β2-microglobulina, coeficiente de tamizado de albúmina de al menos 50.

公开(公告)号：CA2537209C

公开(公告)日：2013-05-14

申请号：CA2537209

申请日：2004-09-06

Applicant: TORAY INDUSTRIES

Inventor： WADA SHIGEHISA ,  UTSUMI JUN ,  FUJITA YOSHIJI ,  SUGAYA HIROYUKI ,  YAMADA SATOKO

IPC: C07K1/14 ,  B01D63/02 ,  B01D69/02 ,  C12M1/00 ,  C12P1/00 ,  G01N1/40

Abstract: The invention provides a method of preparing a biological components-containing solution suitable for clinical proteome analysis by mass spectrometry, electrophoresis, liquid chromatography or the like and an apparatus for the method. The method of the invention is a method for combining two steps among steps of (1) adsorbing proteins having a molecular weight equal to or higher than that of albumin; (2) fractionating proteins having a molecular weight equal to or higher than that of albumin; and (3) concentrating proteins; or a method for preparing the solution by separation using a membrane separation unit having a comparative permeation ratio of 50 or higher for proteins with a molecular weight less than 15,000 and proteins with a molecular weight of 60,000 or higher; or a method for preparing the solution by introducing a biological components-containing raw solution into a separation membrane module and successively passing a diluting solution for circulating the solution and passing a portion of the solution through a separation membrane.

公开(公告)号：EP1661906A4

公开(公告)日：2006-09-06

申请号：EP04787627

申请日：2004-09-06

Applicant: TORAY INDUSTRIES

Inventor： WADA SHIGEHISA ,  UTSUMI JUN ,  FUJITA YOSHIJI ,  SUGAYA HIROYUKI ,  YAMADA SATOKO

IPC: C07K1/34 ,  B01D63/02 ,  B01D69/02 ,  C07K1/14 ,  C07K1/36 ,  C12M1/00 ,  C12P1/00 ,  G01N1/40

CPC classification number: B01D63/02 ,  B01D69/02 ,  G01N1/4005 ,  G01N33/6842 ,  G01N2001/4016 ,  Y10T436/10 ,  Y10T436/25

Abstract: A method of preparing a solution comprising combining two steps of (1) a step of adsorbing a protein having a molecular weight higher than that of albumin, (2) a step of fractionating a protein having a molecular weight higher than that of albumin, and (3) a step of concentrating a protein; a method of preparing a solution comprising separating by the use of a membrane separation unit exhibiting a permeation ratio of a protein having a molecular weight less than 15 thousands to a protein having a molecular weight of 60 thousands or more of 50 or more; and a method of preparing a solution which comprises feeding a raw biological component solution and then a liquid for dilution to a separating membrane module, and allowing a part of the resultant solution to permeate through a separation membrane while circulating the solution. The method can be advantageously employed for preparing a biological component- containing solution which is suitable for the clinical proteomics using mass analysis, electrophoresis, liquid chromatography, and the like.

Abstract translation: 一种制备溶液的方法，该方法包括组合以下两个步骤：（1）吸附分子量高于白蛋白的蛋白质的步骤;（2）分级分子量高于白蛋白的蛋白质的步骤;以及 （3）浓缩蛋白质的步骤; 制备溶液的方法，该方法包括通过使用显示分子量小于15,000的蛋白质对分子量为60,000或更大的蛋白质的透过率为50或更大的膜分离单元进行分离; 以及制备溶液的方法，所述方法包括：将粗生物组分溶液，然后将用于稀释的液体供给到分离膜组件，并且使得到的溶液的一部分在循环溶液的同时通过分离膜渗透。 该方法可以有利地用于制备适用于使用质量分析，电泳，液相色谱等的临床蛋白质组学的含生物成分的溶液。