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公开(公告)号：ES2380538T3
公开(公告)日：2012-05-16
申请号：ES04787627
申请日：2004-09-06
Applicant: TORAY INDUSTRIES
Inventor： WADA SHIGEHISA , UTSUMI JUN , FUJITA YOSHIJI , SUGAYA HIROYUKI , YAMADA SATOKO
IPC: C07K1/22 , B01D63/02 , B01D69/02 , C07K1/14 , C07K1/34 , C07K1/36 , C12M1/00 , C12P1/00 , G01N1/40 , G01N33/68
Abstract: Un procedimiento de preparación de una solución para análisis proteómico que tiene una composición cambiada de componentes biológicos en la que la proporción de concentración de albúmina en las proteínas totales es menor de 0,3 y la proporción de concentración de β2-microglobulina en las proteínas totales es al menos 10 veces más alta que la proporción de concentración en la solución de partida que contiene componentes biológicos, comprendiendo el procedimiento someter la solución que contiene componentes biológicos a tratamiento en al menos dos etapas; en el que las dos etapas se seleccionan entre (1) una etapa de adsorción de una parte o todas las proteínas que tienen un peso molecular igual o superior al de la albúmina; (2) una etapa de retiro de una parte o todas las proteínas que tienen un peso molecular igual o superior al de la albúmina mediante fraccionamiento con un tamiz molecular; y (3) una etapa de concentración de proteínas, en donde se emplea en la etapa (1) o la etapa (2) una membrana de separación de fibra hueca de polisulfona que contiene canales de flujo que tienen una estructura asimétrica que se deriva de polisulfona y poli(vinilpirrolidona) y tiene una proporción de permeación comparativa de β2-microglobulina con respecto a albúmina, definida como coeficiente de tamizado de β2-microglobulina, coeficiente de tamizado de albúmina de al menos 50.
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公开(公告)号：AT540050T
公开(公告)日：2012-01-15
申请号：AT04787627
申请日：2004-09-06
Applicant: TORAY INDUSTRIES
Inventor： WADA SHIGEHISA , UTSUMI JUN , FUJITA YOSHIJI , SUGAYA HIROYUKI , YAMADA SATOKO
IPC: C07K1/22 , B01D63/02 , B01D69/02 , C07K1/14 , C07K1/34 , C07K1/36 , C12M1/00 , C12P1/00 , G01N1/40 , G01N33/68
Abstract: The invention provides a method of preparing a biological components-containing solution suitable for clinical proteome analysis by mass spectrometry, electrophoresis, liquid chromatography or the like and an apparatus for the method. The method of the invention is a method for combining two steps among steps of (1) adsorbing proteins having a molecular weight equal to or higher than that of albumin; (2) fractionating proteins having a molecular weight equal to or higher than that of albumin; and (3) concentrating proteins: or a method for preparing the solution by separation using a membrane separation unit having a comparative permeation ratio of 50 or higher for proteins with a molecular weight less than 15, 000 and proteins with a molecular weight of 60, 000 or higher; or a method for preparing the solution by introducing a biological components-containing raw solution into a separation membrane module and successively passing a diluting solution for circulating the solution and passing a portion of the solution through a separation membrane.
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公开(公告)号：CA2553234A1
公开(公告)日：2005-08-04
申请号：CA2553234
申请日：2005-01-20
Applicant: TORAY INDUSTRIES
Inventor： JUNG GIMAN , WADA SHIGEHISA , YAMADA SATOKO , KUROKI NOBUYUKI , KURODA TOSHIHIKO , SUGAYA HIROYUKI , SEKIGUCHI SHUJI , TANAHASHI KAZUHIRO , KUMO ICHIRO
Abstract: The following is disclosed: (1) a membrane fractionator including a filtrati on section, a concentrating section, a recovery section and a liquid feed pump, wherein a flow channel connecting the filtration section, concentrating section and recovery section to each other constitutes a closed circuitry; ( 2) a method of biocomponent separation, characterized in that a sample derived from biocomponents is fed into an antibody adsorption membrane separation system having an antibody capable of adsorbing specified protein internally accommodated in the middle or a rear part of a membrane separation system th at in the absence of antibodies adsorbing proteins, exhibits a permeation ratio between human .alpha.1-microglobulin and human albumin of 1.5 to 1000, there by separating part of the biocomponents; and (3) a method of protein fractionation, comprising bringing a solution containing two or more types o f proteins and water into contact with a hollow yarn separation membrane to thereby attain protein fractionation, characterized in that the fractionatio n solution contains an organic solvent.
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公开(公告)号：CA2553234C
公开(公告)日：2012-07-24
申请号：CA2553234
申请日：2005-01-20
Applicant: TORAY INDUSTRIES
Inventor： TANAHASHI KAZUHIRO , KUMO ICHIRO , KUROKI NOBUYUKI , SUGAYA HIROYUKI , YAMADA SATOKO , WADA SHIGEHISA , JUNG GIMAN , KURODA TOSHIHIKO , SEKIGUCHI SHUJI
Abstract: The following is disclosed: (1) a membrane fractionator including a filtration section, a concentrating section, a recovery section and a liquid feed pump, wherein a flow channel connecting the filtration section, concentrating section and recovery section to each other constitutes a closed circuitry; (2) a method of biocomponent separation, characterized in that a sample derived from biocomponents is fed into an antibody adsorption membrane separation system having an antibody capable of adsorbing specified protein internally accommodated in the middle or a rear part of a membrane separation system that in the absence of antibodies adsorbing proteins, exhibits a permeation ratio between human &agr;1-microglobulin and human albumin of 1.5 to 1000, thereby separating part of the biocomponents; and (3) a method of protein fractionation, comprising bringing a solution containing two or more types of proteins and water into contact with a hollow yarn separation membrane to thereby attain protein fractionation, characterized in that the fractionation solution contains an organic solvent.
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公开(公告)号：CA2537209A1
公开(公告)日：2005-03-31
申请号：CA2537209
申请日：2004-09-06
Applicant: TORAY INDUSTRIES
Inventor： SUGAYA HIROYUKI , YAMADA SATOKO , UTSUMI JUN , FUJITA YOSHIJI , WADA SHIGEHISA
Abstract: A method of preparing a solution comprising combining two steps of (1) a ste p of adsorbing a protein having a molecular weight higher than that of albumin , (2) a step of fractionating a protein having a molecular weight higher than that of albumin, and (3) a step of concentrating a protein; a method of preparing a solution comprising separating by the use of a membrane separati on unit exhibiting a permeation ratio of a protein having a molecular weight le ss than 15 thousands to a protein having a molecular weight of 60 thousands or more of 50 or more; and a method of preparing a solution which comprises feeding a raw biological component solution and then a liquid for dilution t o a separating membrane module, and allowing a part of the resultant solution to permeate through a separation membrane while circulating the solution. The method can be advantageously employed for preparing a biological component- containing solution which is suitable for the clinical proteomics using mass analysis, electrophoresis, liquid chromatography, and the like.
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Patent Agency Ranking
公开(公告)号：ES2605406T3
公开(公告)日：2017-03-14
申请号：ES05703866
申请日：2005-01-20
Applicant: TORAY INDUSTRIES
Inventor： TANAHASHI KAZUHIRO , KUMO ICHIRO , KUROKI NOBUYUKI , SUGAYA HIROYUKI , YAMADA SATOKO , WADA SHIGEHISA , JUNG GIMAN , KURODA TOSHIHIKO , SEKIGUCHI SHUJI
Abstract: Un dispositivo de fraccionamiento para separar solutos en un liquido bruto mediante una membrana, que comprende 1) una parte de suministro (2b) para cargar el liquido bruto; 2) una parte de filtracion con una membrana (5a; 5b; 5c) para filtrar algo de los solutos en el liquido bruto enviado desde la parte de suministro; 3) una parte de concentracion (5d) para concentrar el filtrado procedente de la parte de filtracion; 4) una parte de recuperacion (10) para recuperar la solucion concentrada obtenida en la parte de concentracion; 5) una bomba de flujo para el envio de una fase movil; en el que la bomba de flujo es una bomba de tubo provista de un rotor giratorio y de un rodillo (9) instalado de manera giratoria en la circunferencia exterior del rotor; caracterizado por que la parte de suministro, la parte de filtracion, la parte de concentracion, la parte de recuperacion y un canal de flujo que conecta las partes respectivas, forman un circuito cerrado y estan ensamblados en un cartucho desechable (14) en el que al menos las porciones de los canales de flujo estan expuestas a la pared exterior del cartucho desde el exterior del cartucho, y por que el cartucho se puede separar del rodillo de la bomba de flujo y esta dispuesto de manera que una parte de la pared exterior del cartucho funciona como un miembro de compresion mediante el acoplamiento de la bomba de rodillos para comprimir las porciones expuestas de los canales de flujo cuando el rodillo se hace girar para hacer circular los liquidos en las partes respectivas del cartucho.
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公开(公告)号：CA2537209C
公开(公告)日：2013-05-14
申请号：CA2537209
申请日：2004-09-06
Applicant: TORAY INDUSTRIES
Inventor： WADA SHIGEHISA , UTSUMI JUN , FUJITA YOSHIJI , SUGAYA HIROYUKI , YAMADA SATOKO
Abstract: The invention provides a method of preparing a biological components-containing solution suitable for clinical proteome analysis by mass spectrometry, electrophoresis, liquid chromatography or the like and an apparatus for the method. The method of the invention is a method for combining two steps among steps of (1) adsorbing proteins having a molecular weight equal to or higher than that of albumin; (2) fractionating proteins having a molecular weight equal to or higher than that of albumin; and (3) concentrating proteins; or a method for preparing the solution by separation using a membrane separation unit having a comparative permeation ratio of 50 or higher for proteins with a molecular weight less than 15,000 and proteins with a molecular weight of 60,000 or higher; or a method for preparing the solution by introducing a biological components-containing raw solution into a separation membrane module and successively passing a diluting solution for circulating the solution and passing a portion of the solution through a separation membrane.
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公开(公告)号：CA2129436A1
公开(公告)日：1994-06-05
申请号：CA2129436
申请日：1993-12-03
Applicant: TORAY INDUSTRIES
Inventor： YAMADA SATOKO , KATAOKA HIROSHI
IPC: A61K38/00 , C07K14/47 , C07K14/52 , C07K14/475 , A61K35/16 , A61K38/18 , A61M1/16 , A61M1/34 , C07K1/34
Abstract: The present invention relates to hematopoietic inhibiting factor-containing compositions which inhibit colony formation of CFU-E, comprising a substance obtained in the fraction eluting at exclusion limit by gel filtration using a gel having maximum molecular weight fractionation of not less than 200,000 and not more than 1,500,000, of a dialysate of blood which is obtained with a dialysis membrane having albumin sieving coefficient of not less than 0.01, followed by concentration with a membrane having molecular weight fractionation of not more than 5,000, and hematopoietic inhibiting factor-containing compositions which inhibit colony formation of CFU-E can be obtained. It further relates to a process of treating blood in which AI component is so removed as to satisfy AI/AA ? 1/50, wherein AI is the height of the absorbancy value of the hematopoietic inhibiting factor-containing compositions according to claims 1 - 5 and AA is the height of the maximum absorbancy value in a gel filtration pattern of solutes of the dialysate expressed in terms of the absorbancy at 280 nm, and to a dialysis membrane which satisfies AI/AA ? 1/50, wherein AI is the height of the absorbancy value of the hematopoietic inhibiting factor-containing compositions according to claims 1 5 and AA is the height of the maximum absorbancy value in a gel filtration pattern of solutes of the dialysate expressed in terms of the absorbancy at 280 nm. It further relates to drug compositions and a therapeutic agent for erythrocytosis, containing such hematopoietic inhibiting factor as the active ingredient.
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9.发明公开METHOD OF PREPARING SOLUTION HAVING COMPOSITION OF BIOLOGICAL COMPONENTS 有权VERFAHREN ZUR HERSTELLUNG EINERLÖSUNGMIT EINER ZUSAMMENSETZUNG BIOLOGISCHER KOMPONENTEN
公开(公告)号：EP1661906A4
公开(公告)日：2006-09-06
申请号：EP04787627
申请日：2004-09-06
Applicant: TORAY INDUSTRIES
Inventor： WADA SHIGEHISA , UTSUMI JUN , FUJITA YOSHIJI , SUGAYA HIROYUKI , YAMADA SATOKO
CPC classification number: B01D63/02 , B01D69/02 , G01N1/4005 , G01N33/6842 , G01N2001/4016 , Y10T436/10 , Y10T436/25
Abstract: A method of preparing a solution comprising combining two steps of (1) a step of adsorbing a protein having a molecular weight higher than that of albumin, (2) a step of fractionating a protein having a molecular weight higher than that of albumin, and (3) a step of concentrating a protein; a method of preparing a solution comprising separating by the use of a membrane separation unit exhibiting a permeation ratio of a protein having a molecular weight less than 15 thousands to a protein having a molecular weight of 60 thousands or more of 50 or more; and a method of preparing a solution which comprises feeding a raw biological component solution and then a liquid for dilution to a separating membrane module, and allowing a part of the resultant solution to permeate through a separation membrane while circulating the solution. The method can be advantageously employed for preparing a biological component- containing solution which is suitable for the clinical proteomics using mass analysis, electrophoresis, liquid chromatography, and the like.
Abstract translation: 一种制备溶液的方法，该方法包括组合以下两个步骤：（1）吸附分子量高于白蛋白的蛋白质的步骤;（2）分级分子量高于白蛋白的蛋白质的步骤;以及 （3）浓缩蛋白质的步骤; 制备溶液的方法，该方法包括通过使用显示分子量小于15,000的蛋白质对分子量为60,000或更大的蛋白质的透过率为50或更大的膜分离单元进行分离; 以及制备溶液的方法，所述方法包括：将粗生物组分溶液，然后将用于稀释的液体供给到分离膜组件，并且使得到的溶液的一部分在循环溶液的同时通过分离膜渗透。 该方法可以有利地用于制备适用于使用质量分析，电泳，液相色谱等的临床蛋白质组学的含生物成分的溶液。
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公开(公告)号：EP1707573A4
公开(公告)日：2010-07-07
申请号：EP05703866
申请日：2005-01-20
Applicant: TORAY INDUSTRIES
Inventor： TANAHASHI KAZUHIRO , KUMO ICHIRO , KUROKI NOBUYUKI , SUGAYA HIROYUKI , YAMADA SATOKO , WADA SHIGEHISA , JUNG GIMAN , KURODA TOSHIHIKO , SEKIGUCHI SHUJI
CPC classification number: B01D63/04 , B01D61/142 , B01D61/20 , B01D63/08 , B01D2313/243 , B01D2319/022 , C07K1/22 , C07K1/34 , C07K1/36
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