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    METHOD FOR ANALYZING RNA
    1.
    发明专利
    METHOD FOR ANALYZING RNA 未知

    公开(公告)号：CA2784837C

    公开(公告)日：2017-10-10

    申请号：CA2784837

    申请日：2010-12-15

    Applicant: TORAY INDUSTRIES

    Inventor： KURODA TOSHIHIKO ,  NOMURA OSAMU ,  NOBUMASA HITOSHI

    IPC: C12Q1/68 ,  G01N27/447 ,  G06F17/30

    Abstract: Information on expression of a gene in a tissue or cell(s) fixed with a fixative , or whether or not the gene is expressed therein, is analyzed by a method for analyzing RNA extracted from a tissue or cell(s) fixed with a fixative, the method comprising a step wherein whether the RNA satisfies the following equation: Equation: B/A

    ANALYSIS CHIP AND ANALYSIS METHOD
    2.
    发明专利
    ANALYSIS CHIP AND ANALYSIS METHOD 未知

    公开(公告)号：CA2671866C

    公开(公告)日：2014-03-25

    申请号：CA2671866

    申请日：2008-01-23

    Applicant: TORAY INDUSTRIES

    Inventor： NOMURA OSAMU ,  KURODA TOSHIHIKO ,  NOBUMASA HITOSHI ,  MURAO YASUO

    IPC: C40B60/12 ,  C12Q1/68 ,  C40B30/04 ,  C40B40/00 ,  G01N33/543

    Abstract: An analysis chip which has a baseboard having a selectively binding substance immobilized on the surface thereof, a covering member bonded to this baseboard and fine particles contained or injected in a movable state within a space that is formed between the baseboard and the covering member, wherein the surface of the fine particles is coated with a surfactant. Owing to this construction, it is possible to prevent the formation of bubbles inhibiting a selective reaction between a test substance and the selectively binding substance having been immobilized so that data variation and lowering in the sensitivity can be regulated and the reproducibility of the measurement can be elevated.

    METHOD FOR PREPARING ARNA AND METHOD FOR ANALYSIS OF GENE EXPRESSION
    3.
    发明专利
    METHOD FOR PREPARING ARNA AND METHOD FOR ANALYSIS OF GENE EXPRESSION 未知

    公开(公告)号：CA2795436C

    公开(公告)日：2017-12-05

    申请号：CA2795436

    申请日：2011-04-21

    Applicant: TORAY INDUSTRIES

    Inventor： KURODA TOSHIHIKO ,  NOMURA OSAMU ,  NOBUMASA HITOSHI

    IPC: C12N15/00 ,  C12N15/09

    Abstract: The present invention provides a method for preparing aRNA to be used for gene expression analysis from an RNA sample extracted from a tissue or cell(s) fixed with a fixative, wherein, in an amplification step of the RNA sample by reverse transcription and in vitro transcription, the ratio of aminoallyl uridine 5'-triphosphate (AA-UTP) in a nucleotide reagent used in the in vitro transcription is not less than 5 mol% and less than 25 mol% with respect to the total of uridine 5'-triphosphate (UTP) and AA-UTP.

    Analysis chip and analysis method
    5.
    发明专利
    Analysis chip and analysis method 未知

    公开(公告)号：AU2008208342B2

    公开(公告)日：2013-02-07

    申请号：AU2008208342

    申请日：2008-01-23

    Applicant: TORAY INDUSTRIES

    Inventor： NOBUMASA HITOSHI ,  KURODA TOSHIHIKO ,  MURAO YASUO ,  NOMURA OSAMU

    IPC: G01N33/53 ,  C12M1/00 ,  C12N15/09 ,  C12Q1/68 ,  G01N33/543 ,  G01N37/00

    Abstract: An analysis chip which has a baseboard having a selectively binding substance immobilized on the surface thereof, a covering member bonded to this baseboard and fine particles contained or injected in a movable state within a space that is formed between the baseboard and the covering member, wherein the surface of the fine particles is coated with a surfactant. Owing to this construction, it is possible to prevent the formation of bubbles inhibiting a selective reaction between a test substance and the selectively binding substance having been immobilized so that data variation and lowering in the sensitivity can be regulated and the reproducibility of the measurement can be elevated.

    METHOD FOR ANALYZING RNA
    8.
    发明专利
    METHOD FOR ANALYZING RNA 未知

    公开(公告)号：CA2784837A1

    公开(公告)日：2011-06-23

    申请号：CA2784837

    申请日：2010-12-15

    Applicant: TORAY INDUSTRIES

    Inventor： KURODA TOSHIHIKO ,  NOMURA OSAMU ,  NOBUMASA HITOSHI

    IPC: C12Q1/68 ,  G01N27/447 ,  G06F17/30

    Abstract: A method for analyzing RNA that is extracted from a tissue or cell that has been fixed with a fixing solution, which comprises a step for determining whether or not said RNA satisfies the formula: B/A=1 [wherein A represents the ratio (%) by weight, in electrophoresis, of RNA having 1000-4000 nucleotides to the total weight of said RNA; B represents the ratio (%) by weight, in electrophoresis, of RNA having more than 4000 nucleotides to the total weight of said RNA]. According to this analysis method, the expression data or the occurrence or non-occurrence of the expression of a gene in the tissue or cell, which has been fixed with the fixing solution, are analyzed.

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