Abstract:
The invention relates to a microplate reader (1) and a respective method, wherein the microplate reader (1) comprises at least one measuring device (2',2",2"') and a holding device (5) for accommodating at least one microplate (4) and for positioning the samples-containing wells (3) of this(these) microplate(s) (4) in relation to the at least one measuring device (2',2",2"'). The at least one measuring device (2',2",2"') is used for detecting light which is emitted by samples in wells (3) of a microplate (4) used resp. inserted in this microplate reader (1) and/or which is influenced by samples transilluminated by light in wells (3) of a microplate (4) used resp. inserted in this microplate reader (1). The micro-plate reader (1) according to the invention is characterized in that it comprises a control unit (6) for controlling the composition of a gas atmosphere (7) surrounding the wells (3) containing the samples of microplates (4) used in this microplate reader (1). A respective use is characterized particularly in that living cells are measured in a controlled gas atmosphere (7), wherein the living cells are chosen from a group which comprises microaerophilic, optionally anaerobic and obligatorily anaerobic micro-organisms as well as fungi and eukaryotic cells.
Abstract:
Haltevorrichtung (1) für zumindest einen Träger (2) der Materialien wie Glas, Kunststoff, Silizium, pyrolytischen Graphit und/oder Metall umfasst, wobei der Träger (2) zur Aufnahme von einer oder mehreren organischen und/oder anorganischen Proben (3) geeignet ist, wobei diese Haltevorrichtung (1) Angriffsflächen (4), die durch Greifer eines Roboters beaufschlagbar sind, und je zwei im Wesentlichen parallel zu einander verlaufende Längswände (7) und Querwände (8) umfasst. Erfindungsgemässe Haltevorrichtungen sind dadurch gekennzeichnet, dass sie rahmenförmig gebaut sind, indem der Bereich zwischen den Längs- und Querwänden als eine, die Vorrichtung vollständig durchdringende Öffnung ausgebildet ist bzw. dass sie plattenförmig gebaut sind, indem der Bereich zwischen den Längs- und Querwänden als Tragfläche ausgebildet ist, welche zumindest eine Vertiefung (11) zur Aufnahme eines Trägers (2) und im Wesentlichen parallel zu den Längswänden (7) und/oder Querwänden (8) verlaufende Zwischenwände (9) umfasst und dass jeweils die beiden Längs- und die beiden Querwände an ihrem Aussenflächenprofil (5) zumindest je eine Angriffsfläche (4) aufweisen.
Abstract:
Betrifft eine Mikroküvetten-Anordnung (1), umfassend eine erste Teilplatte (2) mit einer oder mehreren ersten Küvettenflächen (3) und eine gegenüber der ersten anordenbare, zweite Teilplatte (4) mit mindestens einer oder mehreren zweiten Küvettenflächen (5), die in einer Aktivposition der Mikroküvetten-Anordnung (1) planparallel zu den ersten Küvettenflächen (3) im Register angeordnet und um eine Distanz (6) von diesen beabstandet sind, wodurch in der Aktivposition der Mikroküvetten-Anordnung (1) eine oder mehrere Mikroküvetten (7) ausgebildet sind, in denen je ein vorgängig auf eine der Küvettenflächen (3,5) aufgebrachtes Flüssigkeitsvolumen (8) zwischen diesen beiden Küvettenflächen (3,5) gehalten wird. Die erfindungsgemässe Mikroküvetten-Anordnung (1) ist dadurch gekennzeichnet, dass jede der ersten Küvettenflächen (3) der ersten Teilplatte (2) individuell und vollständig durch eine Oberfläche je eines als freistrahloptisches Element ausgebildeten, in je einer die erste Teilplatte (2) durchdringenden Öffnung (9) angeordneten, transparenten Körpers (10) gebildet ist. Offenbart wird zudem die Verwendung dieser Mikroküvetten-Anordnung (1) bei der Durchführung eines Verfahrens zum Untersuchen von biologischen Proben.
Abstract:
The invention relates to a holding device (1) for at least one support (2) that comprises materials such as glass, plastic, silicon, pyrolytic graphite and/or metal, said support (2) being adapted to receive one or more organic and/or inorganic samples (3). The holding device (1) comprises contact surfaces (4) that can be impinged upon by the grippers of a robot, and two longitudinal walls (7) and transverse walls (8) each that extend substantially in parallel. The inventive holding devices are further characterized in that they have a frame-like design, the area between the longitudinal walls and the transverse walls being configured as an opening that extends completely through the device. The holding devices can also have a plate-shaped design, the area between the longitudinal walls and transverse walls being configured as a support area that comprises at least one recess (11) for receiving a support (2) and dividing walls (9) that extend substantially parallel to the longitudinal walls (7) and/or the transverse walls (8). The two longitudinal walls and the two transverse walls have at least one contact surface (4) each on their outer surface contour (5).
Abstract:
A temperature equalizing plate (1) for a micro plate reader (4), onto which temperature equalizing plate (1) a microplate with wells can be removably arranged, the temperature equalizing plate (1) comprising a plate-shaped body, wherein a first vertical offset is provided between four upward facing corner surfaces (100) and four upward facing edge surfaces (110) and wherein a second vertical offset is provided between the four upward facing edge surfaces (110) and one upward facing center surface (120), resulting in gaps between at least some of the upward facing surfaces (100;110;120) of the temperature equalizing plate (1) and corresponding wells of the thereon arranged microplate.
Abstract:
A system 100 and method are provided for imaging a sample in a sample holder. For providing autofocus, a 2D pattern is projected onto the sample holder 050 via an astigmatic optical element 120. Image data 172 of the sample is acquired by an image sensor 140 via magnification optics 150. A difference in sharpness of the two-dimensional pattern in the image data is measured along a first axis and a second axis. Based on the difference, a magnitude and direction of defocus of the camera subsystem is determined with respect to the sample holder. This enables the sample holder, and thereby the sample, to be brought into focus in a fast and reliable manner.
Abstract:
The invention relates to a holding device (1) for at least one support (2) that comprises materials such as glass, plastic, silicon, pyrolytic graphite and/or metal, said support (2) being adapted to receive one or more organic and/or inorganic samples (3). The holding device (1) comprises contact surfaces (4) that can be impinged upon by the grippers of a robot, and two longitudinal walls (7) and transverse walls (8) each that extend substantially in parallel. The inventive holding devices are further characterized in that they have a frame-like design, the area between the longitudinal walls and the transverse walls being configured as an opening that extends completely through the device. The holding devices can also have a plate-shaped design, the area between the longitudinal walls and transverse walls being configured as a support area that comprises at least one recess (11) for receiving a support (2) and dividing walls (9) that extend substantially parallel to the longitudinal walls (7) and/or the transverse walls (8). The two longitudinal walls and the two transverse walls have at least one contact surface (4) each on their outer surface contour (5).