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    EXTRACTION METHOD FOR HYDROPHILIC RECOMBINANT PROTEIN
    93.
    发明申请
    EXTRACTION METHOD FOR HYDROPHILIC RECOMBINANT PROTEIN 审中-公开
    水解重组蛋白的提取方法

    公开(公告)号：US20150329587A1

    公开(公告)日：2015-11-19

    申请号：US14655017

    申请日：2013-12-18

    Applicant: SPIBER INC.

    Inventor： Toshiaki OSAWA ,  Keisuke MORITA

    IPC: C07K1/14 ,  C07K14/78 ,  C07K14/435

    CPC classification number: C07K1/145 ,  C07K14/43518 ,  C07K14/78

    Abstract: The present invention relates to a method for extracting a hydrophilic recombinant protein from a host expressing a hydrophilic recombinant protein. The method includes: a lysate acquisition step in which a dissolving solvent is added to the host expressing a hydrophilic recombinant protein so as to obtain a host cell lysate; and a step in which a diluting solvent is added to the host cell lysate obtained in the lysate acquisition step, an insoluble substance is separated from the obtained dilution, and a supernatant containing the hydrophilic recombinant protein is collected. The hydrophilic recombinant protein has a hydropathy index of 0 or less. The dissolving solvent is an aprotic polar solvent. The diluting solvent is an aqueous solvent.

    Abstract translation: 本发明涉及从表达亲水重组蛋白的宿主中提取亲水性重组蛋白的方法。 该方法包括：裂解物获取步骤，其中向表达亲水性重组蛋白的宿主中加入溶解溶剂以获得宿主细胞裂解物; 在稀释溶液收集步骤中得到的宿主细胞裂解物中加入稀释溶剂的步骤，从得到的稀释液中分离不溶物质，收集含有亲水性重组蛋白质的上清液。 亲水重组蛋白的亲水指数为0以下。 溶解溶剂是非质子极性溶剂。 稀释溶剂是水性溶剂。

    SOLUTION-DYED PROTEIN FIBER AND METHOD FOR PRODUCING SAME
    94.
    发明申请
    SOLUTION-DYED PROTEIN FIBER AND METHOD FOR PRODUCING SAME 有权
    溶液型蛋白纤维及其制造方法

    公开(公告)号：US20150141618A1

    公开(公告)日：2015-05-21

    申请号：US14405101

    申请日：2013-04-26

    Applicant: SPIBER INC.

    Inventor： Mizuki Ishikawa ,  Kazuhide Sekiyama

    IPC: D01F4/02 ,  D01D1/02 ,  D01D5/06 ,  C07K14/435 ,  D01D5/04

    CPC classification number: D01F4/02 ,  C07K14/43518 ,  D01D1/02 ,  D01D5/04 ,  D01D5/06 ,  D01F1/06 ,  D01F4/00

    Abstract: A solution-dyed protein fiber of the present invention includes 0-100 mass % of silk fibroin and 100-0 mass % of a polypeptide derived from spider silk proteins when the protein fiber is assumed to be 100 mass %, wherein the solution-dyed protein fiber contains a solution-dyeing colorant. The fiber is obtained by: dissolving or dispersing a solution-dyeing colorant in a solvent used for a spinning solution or in dimethyl sulfoxide, thereby preparing a coloring liquid; adding a solvent to the coloring liquid in an amount necessary for a spinning solution; adding and dissolving protein powder into the solvent, thereby preparing a spinning solution; and subjecting the spinning solution to wet spinning or dry-wet spinning. Thereby, the present invention provides a low-cost solution-dyed protein fiber in which a solution-dyeing colorant is dispersed uniformly and that can exhibit bright color tone, and a method for producing the same.

    Abstract translation: 当蛋白质纤维假定为100质量％时，本发明的溶液染色蛋白质纤维包含0-100质量％的丝素蛋白和100-0质量％的衍生自蜘蛛丝蛋白的多肽，其中溶液染色 蛋白质纤维含有溶液染色剂。 纤维通过以下方法获得：将溶染染色剂溶解或分散在用于纺丝溶液或二甲基亚砜的溶剂中，从而制备着色液体; 以纺丝溶液所需的量向着色液中加入溶剂; 将蛋白粉加入溶解溶剂中，制备纺丝溶液; 并对纺丝溶液进行湿纺或干湿纺丝。 因此，本发明提供了一种低成本的溶染染色蛋白质纤维，其中染色着色剂均匀分散并且可以表现出明亮的色调，及其制备方法。

    Polar solvent solution and production method thereof
    99.
    发明授权
    Polar solvent solution and production method thereof 有权

    公开(公告)号：US11668024B2

    公开(公告)日：2023-06-06

    申请号：US16740106

    申请日：2020-01-10

    Applicant: SPIBER INC.

    Inventor： Kana Ishida ,  Hironori Yamamoto ,  Hiroaki Suzumura ,  Kazuhide Sekiyama

    IPC: D01F4/02 ,  D01C3/00 ,  C07K14/435 ,  D01F1/02

    CPC classification number: D01F4/02 ,  C07K14/435 ,  C07K14/43518 ,  D01C3/00 ,  D01F1/02

    Abstract: A polar solvent solution of the present invention is a polar solvent solution in which a solute containing a polyamine acid is dissolved in a polar solvent. The solution has a moisture content of less than 5 mass % based on 100 mass % of the solution. A method for producing a polar solvent solution of the present invention includes changing a moisture content of the solution to adjust the viscosity of the solution. Further, another method for producing a polar solvent solution includes reducing a moisture content of the solution to increase the viscosity of the solution. Thus, the present invention provides a polar solvent solution that enables stable spinning and casting without lowering its viscosity when used as dopes for spinning, film, etc., and methods for producing the same.

    Protein Adhesive, Joined Body, and Method for Producing the Same
    100.
    发明申请
    Protein Adhesive, Joined Body, and Method for Producing the Same 有权

    公开(公告)号：US20220332993A1

    公开(公告)日：2022-10-20

    申请号：US17764670

    申请日：2020-09-30

    Applicant: Spiber Inc.

    Inventor： Yun-Hsiang Chang

    IPC: C09J189/00 ,  C09J7/10

    Abstract: An object of the present invention is to provide a protein adhesive containing a protein, a joined body, and a method for producing the same. The protein adhesive of the present invention contains a protein of 50 KDa or less. The joined body of one embodiment is a joined body including a first object, a second object, and an adhesive layer interposed between the first object and the second object, in which the first object and the second object are joined by the adhesive layer, and the adhesive layer is an adhesive layer formed of a protein adhesive containing a protein of 50 KDa or less.

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