公开(公告)号：US20220324923A1

公开(公告)日：2022-10-13

申请号：US17764669

申请日：2020-09-28

Applicant: Spiber Inc.

Inventor： Kentaro Takahashi ,  Atsushi Ikeda ,  Hideki Kagata ,  Yunosuke Abe ,  Kazuki Sakata

IPC: C07K14/435 ,  D01F4/02

Abstract: An object of the present invention is to provide a method for advantageously producing a protein molded article which can solve a problem caused by esterification of a hydroxyl group contained in a protein while maintaining a sufficient strength. A method for producing a protein molded article according to the present invention includes a step of bringing a raw material molded article containing a protein in which a hydroxyl group is esterified into contact with an acidic or basic medium in a state of applying a tensile force, thus hydrolyzing an ester group.

公开(公告)号：US20220195479A1

公开(公告)日：2022-06-23

申请号：US17600033

申请日：2020-04-01

Applicant: Spiber Inc.

Inventor： Masato KOBAYASHI ,  Kenji KURACHI ,  Eiji MORI ,  Takanobu NODA

IPC: C12P21/02 ,  C12N1/20

Abstract: The present invention relates to a method for producing a recombinant protein, the method including: a first step of culturing a recombinant cell expressing a recombinant protein under control of an inducible promoter in a medium containing galactose and at least two selected from the group consisting of mannitol, raffinose, and melibiose; and a second step of further culturing the recombinant cell under a condition that expression from the inducible promoter is induced after the first step.

公开(公告)号：US20220127755A1

公开(公告)日：2022-04-28

申请号：US17428136

申请日：2020-02-07

Applicant: Spiber Inc.

Inventor： Hirotada Ando ,  Hideto Ishii ,  Koichi Kotaka ,  Ryoko Sato ,  Hiroshi Kano ,  Jun Kobayashi ,  Akito Sato ,  Yunosuke Abe

IPC: D01D5/06 ,  C07K14/435 ,  D01F4/02

Abstract: An object of the present invention is to provide a method for producing an artificial structural protein fiber having a small diameter and having a stress equal to or higher than that of the related art. A method for producing an artificial structural protein fiber according to the present invention is a method for producing an artificial structural protein fiber by a wet spinning method, the method including a coagulation step of discharging a spinning dope containing an artificial structural protein and an organic solvent from a spinneret into a coagulation liquid to coagulate the artificial structural protein, wherein a bath draft in the coagulation step is more than 0.4 and 20 or less.

公开(公告)号：US11306126B2

公开(公告)日：2022-04-19

申请号：US16371932

申请日：2019-04-01

Applicant: SPIBER INC.

Inventor： Kaori Sekiyama ,  Mizuki Ishikawa ,  Shinya Murata

IPC: C07K14/435 ,  C08J5/18 ,  C08L89/00 ,  B29C39/02 ,  B29C55/02 ,  B29C71/02 ,  A61K38/00 ,  B29L7/00

Abstract: A film of the present invention contains a polypeptide derived from spider silk proteins. The decomposition temperature of the film is 240 to 260° C. The film absorbs ultraviolet light having a wavelength of 200 to 300 nm and has a light transmittance of 85% or more at a wavelength of 400 to 780 nm. The film is transparent and colorless in a visible light region. A method for producing a film of the present invention includes: dissolving a polypeptide derived from spider silk proteins in a dimethyl sulfoxide solvent to prepare a dope; and cast-molding the dope on a surface of a base. Thus, the present invention provides a spider silk protein film that can be formed easily and has favorable stretchability, and a method for producing the same.

公开(公告)号：US20210388537A1

公开(公告)日：2021-12-16

申请号：US17279083

申请日：2019-09-27

Applicant: Shima Seiki Mfg., Ltd. ,  Spiber Inc.

Inventor： Shozo Torigoe ,  Akihiko Ozeki

IPC: D02G3/02 ,  D02G1/00 ,  D01F4/02 ,  D06M11/05

Abstract: An object of the present invention is to provide a method for producing protein spinning capable of securing a stable strength by securing sufficient interlacing between fibers. The method for producing a protein spun yarn of the present invention includes a step (a) of preparing a raw material spun yarn including an uncrimped artificial fibroin fiber containing modified fibroin and a step (b) of bringing the raw material spun yarn into contact with an aqueous medium to crimp the artificial fibroin fiber.

公开(公告)号：US20210017672A1

公开(公告)日：2021-01-21

申请号：US16982612

申请日：2019-03-20

Applicant: SHIMA SEIKI MFG., LTD. ,  SPIBER INC.

Inventor： Shozo TORIGOE ,  Seiji SHIMODA ,  Akihiko OZEKI

IPC: D01F4/02 ,  C07K14/435 ,  D02G1/00 ,  D06M15/15

Abstract: Protein fiber having crimp property such that the protein fiber crimps in response to a stimulus is immersed in a solution of protein having a different composition from the protein. The protein having a different composition is infiltrated into the protein fiber and the protein fiber is made crimped.

公开(公告)号：US20200308552A1

公开(公告)日：2020-10-01

申请号：US16649804

申请日：2018-09-28

Applicant: Spiber Inc.

Inventor： Masahiro Murakami

IPC: C12N5/10 ,  C12P21/02 ,  C12N15/11

Abstract: Disclosed is an expression cassette comprising, in a 5′ to 3′ direction of a sense strand, a promoter, and a first nucleic acid, a terminator and a second nucleic acid operably linked to the promoter, wherein the first nucleic acid and the second nucleic acid each contain at least one gene.

公开(公告)号：US20200165311A1

公开(公告)日：2020-05-28

申请号：US16611315

申请日：2018-05-09

Applicant: Spiber Inc. ,  Kojima Industries Corporation

Inventor： Ayumi Abe ,  Keisuke Morita ,  Kenji Kurachi ,  Yunosuke Abe

IPC: C07K14/47 ,  C08L89/00 ,  D01F4/00 ,  D01D5/06

Abstract: The present invention relates to a polypeptide solution in which polypeptide including an amino acid sequence derived from type I keratin that belongs to Cluster Ia is dissolved in a solvent including a formic acid or a solvent including an aprotic polar agent having a dipole moment of 3.0 D or more and an inorganic salt.

公开(公告)号：US20200043085A1

公开(公告)日：2020-02-06

申请号：US16600472

申请日：2019-10-12

Applicant: SPIBER INC.

Inventor： Kazuhide Sekiyama ,  Goshi Uhira

IPC: G06Q30/06 ,  G06F3/0484

Abstract: In an information processing apparatus, a display control part causes a display part to display one or more human models in which parameters relating to shapes and sizes are set. A model selection reception part receives, from a user, a selection operation for selecting one human model from the one or more human models. A parameter changing part changes the parameters set for the human model in conjunction with a change operation from the user for the selected human model.

公开(公告)号：US20190329526A1

公开(公告)日：2019-10-31

申请号：US16469887

申请日：2017-12-15

Applicant: SPIBER INC. ,  KOJIMA INDUSTRIES CORPORATION

Inventor： Tatsuo ONO ,  Ken IMAMURA ,  Shinya UEDA

IPC: B32B5/26

Abstract: The toughness of a laminate is improved. A fiber-reinforced resin material contains a fiber material in a resin; and the fiber material contains high-rigidity fibers and fibroin fibers. According to the present invention, a laminate is formed by laminating and bonding a plurality of fiber-reinforced resin layers. A fiber-reinforced resin layer contains the high-rigidity fibers in a resin. A fiber-reinforced resin layer contains the fibroin fibers in a resin.