公开(公告)号：US10953404B1

公开(公告)日：2021-03-23

申请号：US16858282

申请日：2020-04-24

Applicant: Pattern Bioscience, Inc.

Inventor： Ross Johnson ,  Jonathan Isom ,  David Bussian ,  Nicolas Arab

IPC: B01L3/00 ,  B01L9/00 ,  B01L1/02 ,  G01N21/31

Abstract: An apparatus for loading and imaging a microfluidic chip can comprise a housing having walls that define a vacuum chamber and a first receptacle disposed within the vacuum chamber, the first receptacle defining a space for receiving one or more microfluidic chips. The apparatus can also include a negative pressure source, a light source, and an optical sensor coupled to the housing. The negative pressure source can be configured to reduce pressure within the vacuum chamber, the light source can be positioned to illuminate at least a portion of the space for receiving the chip(s), and the optical sensor can be positioned to capture an image of at least a portion of the space for receiving the chip(s).

公开(公告)号：US20210031189A1

公开(公告)日：2021-02-04

申请号：US17063637

申请日：2020-10-05

Applicant: Pattern Bioscience, Inc.

Inventor： Ross JOHNSON

IPC: B01L3/00

Abstract: Disclosed are microfluidic chips and methods of loading the same. Some microfluidic chips include a microfluidic network that has an inlet port, a channel configured to receive liquid from the inlet port, and a droplet-generating region that includes an end of the channel having a transverse dimension, a constant portion extending from the end of the channel and having a constant transverse dimension that is larger than the traverse dimension of the end of the channel, and an expanding portion extending from the constant portion, wherein the transverse dimension of the end of the channel, the transverse dimension of the constant portion, and a length of the constant portion are configured such that, when an aqueous liquid is flowed through the droplet-generating region in the presence of a non-aqueous liquid, droplets of the aqueous liquid are completely formed in the constant portion.

公开(公告)号：US12128415B2

公开(公告)日：2024-10-29

申请号：US17195206

申请日：2021-03-08

Applicant: Pattern Bioscience, Inc.

Inventor： Ross Johnson ,  Jonathan Isom ,  David Bussian ,  Nicolas Arab

IPC: B01L9/00 ,  B01L1/02 ,  B01L3/00 ,  G01N21/31

CPC classification number: B01L9/527 ,  B01L1/02 ,  B01L3/502715 ,  B01L3/50273 ,  B01L3/502784 ,  G01N21/31 ,  B01L2200/0642 ,  B01L2200/0647 ,  B01L2300/0663 ,  B01L2400/049

Abstract: An apparatus for loading and imaging a microfluidic chip can comprise a housing having walls that define a vacuum chamber and a first receptacle disposed within the vacuum chamber, the first receptacle defining a space for receiving one or more microfluidic chips. The apparatus can also include a negative pressure source, a light source, and an optical sensor coupled to the housing. The negative pressure source can be configured to reduce pressure within the vacuum chamber, the light source can be positioned to illuminate at least a portion of the space for receiving the chip(s), and the optical sensor can be positioned to capture an image of at least a portion of the space for receiving the chip(s).

公开(公告)号：US20230243859A1

公开(公告)日：2023-08-03

申请号：US18054326

申请日：2022-11-10

Applicant: Pattern Bioscience, Inc.

Inventor： Chueh-Yu WU

IPC: G01N35/08 ,  B01L3/00

CPC classification number: G01N35/08 ,  B01L3/502784 ,  B01L3/502738 ,  B01L2300/0861 ,  B01L2200/16

Abstract: A microfluidic chip can include a microfluidic network that comprises a port, one or more test volumes, and one or more channels through which fluid must flow from the port to the test volume(s). A crosslinkable material can also be disposed within the microfluidic network such that the crosslinkable material is flowable through the channel(s). The crosslinkable material of the microfluidic chip may be exposed to light and/or heat to crosslink the material within and thereby occlude the channel(s). A method of loading the microfluidic chip can include disposing a liquid within a port of a microfluidic network that includes one or more test volumes and one or more channels; flowing each of one or more portions of the liquid from the port, through at least one of the channel(s), and into a respective one of the test volume(s); and directing a crosslinkable material into at least one of the channel(s) and cross-linking the crosslinkable material such that none of the test volume(s) are in fluid communication with the port when the portion(s) of the liquid are in the test volume(s).

公开(公告)号：US20230033708A1

公开(公告)日：2023-02-02

申请号：US17815957

申请日：2022-07-29

Applicant: thinXXS Microtechnology GmbH ,  Pattern Bioscience, Inc.

Inventor： Lutz WEBER ,  Ross JOHNSON

IPC: B01L3/00

Abstract: A microfluidic device can include a microfluidic circuit that comprises an inlet port, a reagent-containing chamber configured to receive fluid from the inlet port, a non-aqueous-liquid-containing reservoir configured to receive liquid from the chamber, and a droplet-generating region configured to receive and produce droplets of liquid from the reservoir. The circuit can also include first and second valves or frangible members. The first valve or frangible member can have closed position in which fluid is prevented from entering or exiting the chamber therethrough and an open position in which fluid is permitted to enter or exit the chamber therethrough. The second valve or frangible member can have a closed position in which fluid is prevented from flowing between the chamber and the reservoir therethrough and an open position in which fluid is permitted to flow between the chamber and the reservoir therethrough.

公开(公告)号：US11344890B2

公开(公告)日：2022-05-31

申请号：US16661829

申请日：2019-10-23

Applicant: Pattern Bioscience, Inc.

Inventor： Nicolas Arab ,  Ross Johnson ,  David Bussian ,  Jon Isom

IPC: B01L3/00 ,  G01N35/08

Abstract: A microfluidic chip that can have a body defining a microfluidic network including a test volume, one or more ports, and one or more channels in fluid communication between the port(s) and the test volume. Gas can be removed from the test volume before a sample liquid is introduced therein by reducing pressure at a first one of the port(s), optionally while the liquid is disposed in the port. Liquid in the first port can be introduced into the test volume by increasing pressure at the first port. The microfluidic network can define one or more droplet-generating regions in which at least one of the channel(s) defines a constriction and/or two or more of the channels connect at a junction. Liquid flowing from the first port can pass through at least one of the droplet-generating region(s) and to the test volume.

公开(公告)号：US20220118447A1

公开(公告)日：2022-04-21

申请号：US17505129

申请日：2021-10-19

Applicant: Pattern Bioscience, Inc.

Inventor： Ross Johnson

IPC: B01L3/00

Abstract: A microfluidic chip can comprise a body defining a microfluidic network having one or more inlet ports, a test volume, and one or more flow paths extending between the inlet port(s) and the test volume. Along each of the flow path(s), fluid can flow from one of the inlet port(s), through at least one droplet-generating region in which a minimum cross-sectional area of the flow path increases along the flow path, and to the test volume. The network can include a gutter disposed along at least a portion of the test volume's periphery. The gutter can have a depth along a trough that is at least 10% larger than the depth of the test volume at the periphery and a depth along a ridge disposed between the trough and the test volume that is less than the depth of the test volume at the periphery.

公开(公告)号：US20210053065A1

公开(公告)日：2021-02-25

申请号：US16998646

申请日：2020-08-20

Applicant: Pattern Bioscience, Inc.

Inventor： Nicolas Arab ,  Ross Johnson

IPC: B01L3/00 ,  C12M1/12 ,  C12M1/34 ,  C12Q1/04

Abstract: A method of analyzing a sample comprising one or more species of microorganisms can include generating first droplets such that each of one or more microorganisms of a first portion of the sample is encapsulated within one of the first droplets and, for each of one or more aliquots of a second portion of the sample, second droplets such that each of one or more microorganisms of the aliquot is encapsulated within one of the second droplets. First and second sets of data can be captured, the first set indicative of the identity and quantity of encapsulated microorganism(s) of the first portion of the sample and the second set indicative of a phenotypic response of encapsulated microorganism(s) of the aliquot(s) to one or more test reagents. A target species' phenotypic response to the test reagent(s) is determinable at least by referencing the second data set to the first data set.

公开(公告)号：US20200298229A1

公开(公告)日：2020-09-24

申请号：US16661829

申请日：2019-10-23

Applicant: Pattern Bioscience, Inc.

Inventor： Nicolas ARAB ,  Ross JOHNSON ,  David BUSSIAN ,  Jon ISOM

IPC: B01L3/00

Abstract: A microfluidic chip that can have a body defining a microfluidic network including a test volume, one or more ports, and one or more channels in fluid communication between the port(s) and the test volume. Gas can be removed from the test volume before a sample liquid is introduced therein by reducing pressure at a first one of the port(s), optionally while the liquid is disposed in the port. Liquid in the first port can be introduced into the test volume by increasing pressure at the first port. The microfluidic network can define one or more droplet-generating regions in which at least one of the channel(s) defines a constriction and/or two or more of the channels connect at a junction. Liquid flowing from the first port can pass through at least one of the droplet-generating region(s) and to the test volume.

公开(公告)号：US20240425896A1

公开(公告)日：2024-12-26

申请号：US18602861

申请日：2024-03-12

Applicant: PATTERN BIOSCIENCE, INC.

Inventor： Nicolas ARAB ,  Ross JOHNSON

IPC: C12Q1/04 ,  C12Q1/18 ,  G01N15/10 ,  G01N15/14 ,  G01N15/1404 ,  G01N15/1433 ,  G01N21/64 ,  G01N33/50 ,  G01N33/58

Abstract: Certain embodiments of the invention are directed to evaluating and identifying cells by recording and interpreting a time-dependent signal produced by unique cell respiration and permeability attributes of isolated viable cells.