公开(公告)号：US10174313B2

公开(公告)日：2019-01-08

申请号：US15885736

申请日：2018-01-31

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY ,  UNIVERSITY OF SOUTHERN CALIFORNIA ,  CURATE BIOSCIENCES LLC

Inventor： Emil P. Kartalov ,  Cheng-Chung Lee ,  Paul Predki

IPC: C12Q1/68 ,  C12N15/10 ,  C12Q1/686 ,  C12Q1/6874 ,  B01L3/00 ,  C12Q1/6853

Abstract: Provided herein are devices and methods for the micro-isolation of biological cellular material. A micro-isolation device described can comprise a photomask that protects regions of interest against DNA-destroying illumination. The micro-isolation device can further comprise photosensitive material defining access wells following illumination and subsequent developing of the photosensitive material. The micro-isolation device can further comprise a chambered microfluidic device comprising channels providing access to wells defined in photosensitive material. The micro-isolation device can comprise a chambered microfluidic device without access wells defined in photosensitive material where valves control the flow of gases or liquids through the channels of the microfluidic device. Also included are methods for selectively isolating cellular material using the devices described herein, as are methods for biochemical analysis of individual regions of interest of cellular material using the devices described herein. Further included are methods of making masking arrays useful for the methods described herein. The micro-isolation devices can comprise a unique combination of barcodes in each microfluidics well, allowing two-dimensional mapping of genetic information.

公开(公告)号：US20130210646A1

公开(公告)日：2013-08-15

申请号：US13763536

申请日：2013-02-08

Applicant: University of Southern California ,  California Institute of Technology

Inventor： Emil P. Kartalov ,  Michael Kahn ,  Axel Scherer

IPC: C12Q1/68 ,  C12M1/12 ,  C12Q1/24

CPC classification number: C12Q1/686 ,  B01L3/502761 ,  B01L3/50851 ,  B01L7/52 ,  B01L2200/0642 ,  B01L2200/0668 ,  B01L2300/0816 ,  B01L2300/0819 ,  B01L2300/0864 ,  B01L2300/0874 ,  C12M25/00 ,  C12Q1/24 ,  G01N15/14 ,  G01N33/574 ,  G01N2015/1006

Abstract: Described microfluidic technology focused on: 1) direct integration of microfluidic devices with solidified liquid tissue samples, 2) subordination of architectural and operational principles of microfluidic devices specific tissue structure and needs and/or 3) on-chip sample acquisition integrated with the detection measurement within the same device. In contrast to conventional methods of off-chip sample prep and subsequent insertion into a detection device, new applications are possible on solidified liquid or solid tissue samples, such as in situ PCR.

Abstract translation: 描述的微流控技术主要集中在：1）将微流体装置与凝固的液体组织样品直接集成，2）微流体装置的特定组织结构和需要的结构和操作原理的从属性和/或3）集成了检测测量的片上样品采集 在同一设备内。 与片外样品制备和随后插入检测装置的常规方法相比，在固化液体或固体组织样品（例如原位PCR）上可能有新的应用。

公开(公告)号：US12168797B2

公开(公告)日：2024-12-17

申请号：US18352112

申请日：2023-07-13

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY

Inventor： Emil P. Kartalov ,  Aditya Rajagopal ,  Axel Scherer

IPC: G01N33/48 ,  C12Q1/68 ,  C12Q1/6825 ,  C12Q1/6851 ,  G01N21/64 ,  G01N33/50 ,  G16B5/00 ,  G16B25/00 ,  G16B25/20 ,  G16B40/10 ,  G06F17/10 ,  G06F17/11 ,  G16B40/00

Abstract: This disclosure provides methods, systems, compositions, and kits for the multiplexed detection of a plurality of analytes in a sample. In some examples, this disclosure provides methods, systems, compositions, and kits wherein multiple analytes may be detected in a single sample volume by acquiring a cumulative measurement or measurements of at least one quantifiable component of a signal. In some cases, additional components of a signal, or additional signals (or components thereof) are also quantified. Each signal or component of a signal may be used to construct a coding scheme which can then be used to determine the presence or absence of any analyte.

公开(公告)号：US20240167092A1

公开(公告)日：2024-05-23

申请号：US18128764

申请日：2023-03-30

Applicant: California Institute of Technology

Inventor： Emil P. Kartalov ,  Aditya Rajagopal ,  Axel Scherer ,  Mark D. Goldberg

IPC: C12Q1/6883 ,  C12Q1/68 ,  C12Q1/6851 ,  C12Q1/70

CPC classification number: C12Q1/6883 ,  C12Q1/68 ,  C12Q1/6851 ,  C12Q1/701 ,  C12Q1/703 ,  C12Q2600/156 ,  Y10T436/143333

Abstract: FRET-based analytes detection and related methods and systems are described where a pair of FRET labeled primers and/or oligonucleotides are used that are specific for target sequences located at a distance up to four time the Forster distance of the FRET chromophores presented on the FRET labeled primers and/or oligonucleotides one with respect to the other in one or more polynucleotide analyte; in particular the pair of FRET labeled primers and/or oligonucleotides is combined with a sample and subjected to one or more polynucleotide amplification reactions before measuring FRET signals from at least one FRET chromophore.

公开(公告)号：US11827921B2

公开(公告)日：2023-11-28

申请号：US16864744

申请日：2020-05-01

Applicant: California Institute of Technology

Inventor： Emil P. Kartalov ,  Aditya Rajagopal ,  Axel Scherer

IPC: G01N33/48 ,  G01N33/50 ,  C12Q1/6825 ,  G16B25/00 ,  G16B5/00 ,  C12Q1/6851 ,  G16B25/20 ,  G16B40/10 ,  C12Q1/68 ,  G01N21/64 ,  G06F17/10 ,  G06F17/11 ,  G16B40/00

CPC classification number: C12Q1/6825 ,  C12Q1/68 ,  C12Q1/6851 ,  G01N21/6486 ,  G16B5/00 ,  G16B25/00 ,  G16B25/20 ,  G16B40/10 ,  G06F17/10 ,  G06F17/11 ,  G16B40/00 ,  Y02A50/30 ,  C12Q1/6851 ,  C12Q2537/101 ,  C12Q2537/143 ,  C12Q2537/165 ,  C12Q2563/107

Abstract: This disclosure provides methods, systems, compositions, and kits for the multiplexed detection of a plurality of analytes in a sample. In some examples, this disclosure provides methods, systems, compositions, and kits wherein multiple analytes may be detected in a single sample volume by acquiring a cumulative measurement or measurements of at least one quantifiable component of a signal. In some cases, additional components of a signal, or additional signals (or components thereof) are also quantified. Each signal or component of a signal may be used to construct a coding scheme which can then be used to determine the presence or absence of any analyte.

公开(公告)号：US20230086246A1

公开(公告)日：2023-03-23

申请号：US17885037

申请日：2022-08-10

Applicant: California Institute of Technology

Inventor： Emil P. Kartalov ,  Aditya Rajagopal ,  Axel Scherer

IPC: C12Q1/6825 ,  G16B25/00 ,  G16B5/00 ,  C12Q1/6851 ,  G16B25/20 ,  G16B40/10 ,  C12Q1/68 ,  G01N21/64

Abstract: This disclosure provides methods, systems, compositions, and kits for the multiplexed detection of a plurality of analytes in a sample. In some examples, this disclosure provides methods, systems, compositions, and kits wherein multiple analytes may be detected in a single sample volume by acquiring a cumulative measurement or measurements of at least one quantifiable component of a signal. In some cases, additional components of a signal, or additional signals (or components thereof) are also quantified. Each signal or component of a signal may be used to construct a coding scheme which can then be used to determine the presence or absence of any analyte.

公开(公告)号：US11492664B2

公开(公告)日：2022-11-08

申请号：US16051736

申请日：2018-08-01

Applicant: California Institute of Technology

Inventor： Aditya Rajagopal ,  Emil P. Kartalov

IPC: C12Q1/68 ,  C12Q1/686 ,  C07H21/04 ,  G01N21/64

Abstract: The present invention relates to methods of nucleic acid analyte detection by PCR. In particular, methods and kits for the detection of a plurality of nucleic acid analytes and the generation of kinetic signatures are provided. Further provided are methods and kits of nested PCR and PCR using limiting primers.

公开(公告)号：US20210012860A1

公开(公告)日：2021-01-14

申请号：US16937464

申请日：2020-07-23

Applicant: California Institute of Technology

Inventor： Emil P. Kartalov ,  Aditya Rajagopal ,  Axel Scherer

IPC: G16B25/00 ,  G16B5/00 ,  C12Q1/6851 ,  G16B25/20 ,  G16B40/10 ,  C12Q1/68 ,  G01N21/64 ,  C12Q1/6825

Abstract: This disclosure provides methods, systems, compositions, and kits for the multiplexed detection of a plurality of analytes in a sample. In some examples, this disclosure provides methods, systems, compositions, and kits wherein multiple analytes may be detected in a single sample volume by acquiring a cumulative measurement or measurements of at least one quantifiable component of a signal. In some cases, additional components of a signal, or additional signals (or components thereof) are also quantified. Each signal or component of a signal may be used to construct a coding scheme which can then be used to determine the presence or absence of any analyte.

公开(公告)号：US09149805B2

公开(公告)日：2015-10-06

申请号：US13913321

申请日：2013-06-07

Applicant: UNIVERSITY OF SOUTHERN CALIFORNIA ,  APIC CORPORATION ,  CALIFORNIA INSTITUTE OF TECHNOLOGY

Inventor： Emil P. Kartalov ,  Axel Scherer ,  Koichi Sayano

IPC: B01L3/02 ,  B01L3/00 ,  A61M1/36 ,  A61M1/34

CPC classification number: B01L3/502753 ,  A61M1/3472 ,  A61M1/3496 ,  A61M1/3633 ,  A61M2205/0244 ,  B01L2300/0645 ,  B01L2300/0681 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2300/087 ,  B01L2400/0424 ,  Y10T436/25125 ,  Y10T436/25375

Abstract: A microfluidic fluid separator for separating target components of a fluid by filtration is described. Methods for separating target components of a fluid by filtration and methods for processing blood on a large scale with the microfluidic fluid separator are provided.

Abstract translation: 描述了用于通过过滤分离流体的目标成分的微流体流体分离器。 提供了通过过滤分离流体的目标组分的方法和用微流体分离器大规模处理血液的方法。

公开(公告)号：US20230038055A1

公开(公告)日：2023-02-09

申请号：US17958939

申请日：2022-10-03

Applicant: California Institute of Technology

Inventor： Aditya Rajagopal ,  Emil P. Kartalov

IPC: C12Q1/686 ,  C07H21/04

Abstract: The present invention relates to methods of nucleic acid analyte detection by PCR. In particular, methods and kits for the detection of a plurality of nucleic acid analytes and the generation of kinetic signatures are provided. Further provided are methods and kits of nested PCR and PCR using limiting primers.