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    Methods for reprogramming cells and uses thereof
    11.
    发明授权
    Methods for reprogramming cells and uses thereof 有权

    公开(公告)号：US10131879B2

    公开(公告)日：2018-11-20

    申请号：US15298006

    申请日：2016-10-19

    Applicant: GENESIS TECHNOLOGIES LIMITED

    Inventor： Jan-Eric Ahlfors ,  Rouwayda El-Ayoubi

    IPC: C12N5/00 ,  C12N5/0775 ,  C12N5/0797 ,  C12N5/079 ,  C12N5/074 ,  A61K35/30 ,  C12N5/0789 ,  C12N5/077 ,  C12N5/0793 ,  C12N5/071 ,  A61K35/545

    Abstract: An in vitro human neural multipotent, unipotent, or somatic cell possessing all of the following characteristics: is derived from the reprogramming of a somatic cell, a progenitor cell or a stem cell that exhibits at least a transient increase in intracellular levels of at least one reprogramming agent; is not differentiated from a pluripotent cell; expresses one or more markers of a multipotent, unipotent or somatic cell not characteristic of a neural stem cell, neural precursor cell, neural progenitor cell, neuroblast, or neuron; is not a cancerous cell; is stable and not artificially maintained by forced gene expression and may be maintained in standard neural stem cell media or neural media; and does not exhibit uncontrolled growth, teratoma formation, and tumor formation in vivo; wherein the cell comprises at least one polypeptide or an expression vector encoding at least one polypeptide selected from the group consisting of: Musashi1 (Msi1); Ngn2; Msi1 and Ngn2; Msi1 and methyl-CpG binding domain protein 2 (MBD2); Ngn2 and MBD2; Msi1, Ngn2 and MBD2; Achaete-Scute Homolog 1 (Ascl1); Msi1, Ngn2 and Ascl1; Msi1, Ngn2, MBD2 and Ascl1; Sox2; Msi1, Ngn2 and Sox2; and Msi1, Ngn2, MBD2 and Sox2; wherein the expression vector is transiently expressed.

    AUTOMATED CELL PROCESSING SYSTEMS AND METHODS
    15.
    发明公开
    AUTOMATED CELL PROCESSING SYSTEMS AND METHODS 审中-公开

    公开(公告)号：US20240003927A1

    公开(公告)日：2024-01-04

    申请号：US18467374

    申请日：2023-09-14

    Applicant: Genesis Technologies Limited

    Inventor： Jan-Eric Ahlfors

    IPC: G01N35/10 ,  B01L1/02 ,  B01L1/04 ,  G01N35/00

    CPC classification number: G01N35/1002 ,  B01L1/02 ,  B01L1/04 ,  G01N35/0099 ,  B01L2300/06 ,  B01L2300/14 ,  G01N2035/00277 ,  G01N2035/00306 ,  G01N2035/1051

    Abstract: A system for automated processing of a plurality of batches, each batch being derived from one biological sample, the system comprising an enclosure which can be closed and sterilized, each batch of the plurality of batches comprising one or more cell processing container; a plurality of reagent containers for holding reagents within the enclosure; at least one reagent dispenser within the enclosure for dispensing reagents during said automated processing; a quality control system within the enclosure for analyzing at least one characteristic of a batch during said automated processing; a harvester within the enclosure for harvesting batches; a robotic system within the enclosure, configured for transporting cell processing containers, decapping or otherwise opening cell processing containers, pipetting reagents or liquids from cell processing containers, and aspirating liquids from cell processing containers, during said automated processing; a tracker for electronically tracking the plurality of batches after its introduction to the enclosure; and a control unit (CU) communicatively coupled to the at least one reagent dispenser, the quality control system, the harvester, the robotic system and the tracker for controlling said automatic processing of said batches.

    METHODS FOR REPROGRAMMING CELLS AND USES THEREOF
    16.
    发明公开
    METHODS FOR REPROGRAMMING CELLS AND USES THEREOF 审中-公开

    公开(公告)号：US20230227785A1

    公开(公告)日：2023-07-20

    申请号：US18188421

    申请日：2023-03-22

    Applicant: Genesis Technologies Limited

    Inventor： Jan-Eric Ahlfors ,  Rouwayda El-Ayoubi

    IPC: C12N5/0797

    CPC classification number: C12N5/0623 ,  C12N2513/00 ,  C12N2501/115 ,  C12N2501/13 ,  C12N2506/1307 ,  C12N2501/105 ,  C12N2501/60 ,  C12N2501/065 ,  C12N2501/11 ,  C12N2501/998

    Abstract: A method of obtaining a pluripotent-like multipotent cell, including providing a cell of a first type which is not a pluripotent-like multipotent cell; contacting the cell of a first type with an agent capable of remodeling the chromatin and/or DNA of the cell; transiently increasing expression of at least one pluripotent gene regulator in the cell of a first type, to a level at which the at least one pluripotent gene regulator is capable of driving transformation of the cell of a first type into the pluripotent-like multipotent cell; and placing or maintaining the cell in a differentiation medium and maintaining intracellular levels of the at least one pluripotent gene regulator for a sufficient period of time to allow a stable pluripotent-like multipotent cell to be obtained; wherein the pluripotent-like multipotent cell so obtained does not exhibit teratoma formation in vivo.

    METHODS FOR REPROGRAMMING CELLS AND USES THEREOF
    17.
    发明申请
    METHODS FOR REPROGRAMMING CELLS AND USES THEREOF 审中-公开

    公开(公告)号：US20200263140A1

    公开(公告)日：2020-08-20

    申请号：US16752535

    申请日：2020-01-24

    Applicant: GENESIS TECHNOLOGIES LIMITED

    Inventor： Jan-Eric Ahlfors ,  Rouwayda El-Ayoubi

    IPC: C12N5/074 ,  C12N5/0775 ,  C12N5/0797

    Abstract: A method of obtaining a pancreatic multipotent or unipotent cell including providing a cell of a first type which is not a pancreatic multipotent or unipotent cell; contacting the cell of a first type with an agent capable of remodeling the chromatin and/or DNA of the cell; transiently increasing expression of at least one pancreatic multipotent or unipotent gene regulator in the cell of a first type, to a level at which the at least one pancreatic multipotent or unipotent gene regulator is capable of driving transformation of the cell of a first type into the pancreatic multipotent or unipotent cell; and placing or maintaining the cell in a pancreatic cell culture medium and maintaining intracellular levels of the at least one pancreatic multipotent or unipotent gene regulator for a sufficient period of time to allow a pancreatic multipotent or unipotent cell to be obtained.

    Methods for reprogramming cells and uses thereof
    18.
    发明授权
    Methods for reprogramming cells and uses thereof 有权

    公开(公告)号：US10557123B2

    公开(公告)日：2020-02-11

    申请号：US16195005

    申请日：2018-11-19

    Applicant: Genesis Technologies Limited

    Inventor： Jan-Eric Ahlfors ,  Rouwayda El-Ayoubi

    IPC: C12N5/00 ,  C12N5/0775 ,  C12N5/0797 ,  C12N5/079 ,  C12N5/074 ,  A61K35/30 ,  C12N5/0789 ,  C12N5/077 ,  C12N5/0793 ,  C12N5/071 ,  A61K35/545

    Abstract: A method of obtaining a neural multipotent, unipotent or somatic cell, including: i) providing a cell of a first type which is not a neural multipotent, unipotent or somatic cell; ii) increasing expression of at least one neural multipotent or unipotent gene regulator in the cell of a first type, to a level at which the at least one neural multipotent or unipotent gene regulator is capable of driving transformation of the cell of a first type into the neural multipotent, unipotent or somatic cell, wherein the at least one multipotent or unipotent gene regulator is Musashi1 (Msi1), Neurogenin 2 (Ngn2), or both Msi1 and Ngn2; and iii) placing or maintaining the cell in a neural cell culture medium and maintaining sufficient intracellular levels of the at least one multipotent or unipotent gene regulator for a sufficient period of time to allow a stable neural multipotent, unipotent or somatic cell to be obtained.

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