公开(公告)号：US20190299207A1

公开(公告)日：2019-10-03

申请号：US16380693

申请日：2019-04-10

Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA

Inventor： Luke P. Lee ,  Jun Ho Sun

IPC: B01L3/00 ,  C12Q1/686 ,  C12N5/078

Abstract: An integrated molecular diagnostics system (iMDx) for the detection of Dengue Fever at point-of-care, wherein blood plasma separation for the selective separation of the Dengue virus from the whole blood, LED-driven photothermal lysis of the Dengue virus for the RNA extraction, and LED-driven rapid optical cavity PCR for the amplification of Dengue viral RNA are integrated on-chip within a single micro-fluidic device.

公开(公告)号：US20180355298A1

公开(公告)日：2018-12-13

申请号：US15536086

申请日：2015-12-14

Applicant: The Regents of the University of California

Inventor： Peter Loskill ,  Anurag Mathur ,  Kevin E. Healy ,  Luke P. Lee

IPC: C12M3/00 ,  B01L3/00 ,  C12M3/06 ,  C12M1/00 ,  C12M1/36 ,  C12M1/34 ,  C12N5/074 ,  G01N33/50

CPC classification number: C12M21/08 ,  B01L3/502715 ,  B01L2200/027 ,  B01L2200/028 ,  B01L2300/0816 ,  C12M23/16 ,  C12M23/58 ,  C12M41/44 ,  C12M41/48 ,  C12N5/0607 ,  G01N33/5073

Abstract: Multi-organ cell culture systems and methods are provided. Aspects of the cell culture systems include at least two microfluidic cell culture units configured to culture a plurality of cells, one or more connectors configured to fluidly connect the microfluidic cell culture units to one another, a cell culture medium configured to support the growth of a plurality of different cell types, and a controller configured to move the cell culture medium at a specified volumetric flow rate between the microfluidic cell culture units. The subject systems and methods find use in a variety of applications, including in vitro evaluation of candidate agents for toxicity and efficacy, in vitro models of disease, and in vitro models for fundamental studies of biological systems.

公开(公告)号：US20180236451A1

公开(公告)日：2018-08-23

申请号：US15878033

申请日：2018-01-23

Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA

Inventor： Luke P. Lee ,  Jun Ho Son

IPC: B01L7/00 ,  B01L3/00 ,  C12Q1/686

CPC classification number: B01L7/52 ,  B01L3/502707 ,  B01L2300/0893 ,  B01L2300/1827 ,  B01L2300/1861 ,  C12Q1/686

Abstract: An LED-driven optical cavity PCR system and method is disclosed for fast, accurate and reliable PCR based diagnostics. An optical cavity comprising two thin light absorbing metal (AU) films is used for uniform light absorption and subsequent photo thermal light-to-heat conversion is employed for PCR thermal cycling.

公开(公告)号：US20180080064A1

公开(公告)日：2018-03-22

申请号：US15649328

申请日：2017-07-13

Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA

Inventor： Luke P. Lee ,  Jun Ho Son ,  Byungrae Cho

IPC: C12Q1/68 ,  B01L7/00 ,  B01L3/00

CPC classification number: C12Q1/686 ,  B01L3/50851 ,  B01L7/52 ,  B01L2200/147 ,  B01L2300/0829 ,  B01L2300/0896 ,  B01L2300/168 ,  B01L2300/1861 ,  C12Q2565/628

Abstract: Systems and methods for plasmonic heating by combined use of thin plasmonic film-based 2D and 3D structures and a light-emitting diode (LED) for nucleic acids amplification through fast thermal cycling of polymerase chain reaction (PCR) are described.

公开(公告)号：US09737888B2

公开(公告)日：2017-08-22

申请号：US14947893

申请日：2015-11-20

Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA

Inventor： Luke P. Lee ,  Debkishore Mitra ,  John R. Waldeisen ,  Jixiao Liu

IPC: G01N3/00 ,  B01L3/00 ,  B01D19/00

CPC classification number: B01L3/502723 ,  B01D19/0031 ,  B01L3/50273 ,  B01L2200/0684 ,  B01L2300/069 ,  B01L2300/0816 ,  B01L2300/0858 ,  B01L2300/0883 ,  B01L2300/10 ,  B01L2300/12 ,  B01L2300/123 ,  B01L2400/0481 ,  B01L2400/049 ,  B01L2400/0605 ,  B01L2400/0638 ,  B01L2400/0694

Abstract: An apparatus with a self-contained, tunable, microfluidic pumping system that utilizes the high air permeability of the matrix material to actuate fluid flow in a network of fluidic microchannels and microstructures is provided. The pumping relies upon partial evacuation of degas/vacuum channels that are located next to the fluid channels to degas air from the fluid channels or structures producing a reduction of pressure in the fluidic channel leading to the flow of fluid from an inlet at atmospheric pressure through the device. The solution is isolated from the pumping apparatus since the liquid does not pass through the diffusion barriers. The apparatus and method can also provide bubble-free microfluidic pumping, without any auxiliary equipment or device pre-treatment, and can fill dead-end channels and chambers, providing a powerful liquid handling tool for a broad range of applications.

公开(公告)号：US20160228874A1

公开(公告)日：2016-08-11

申请号：US15017851

申请日：2016-02-08

Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA

Inventor： Luke P. Lee ,  Erh-Chia Yeh

IPC: B01L3/00 ,  C12Q1/68 ,  C12Q1/70

Abstract: A Digital Separation (DS) chip for separating, digitizing and analyzing a fluid sample is presented. The DS chip includes a fluidic layer that prepares and compartmentalizes the fluid sample for analysis. Cliff structures that are adjacent to wells skim the fluid sample and prevent particles, which may interfere with fluid sample analysis, from entering the wells. Skimmed fluid sample analysis occurs in the wells and endpoint data can be collected and used to determine an original concentration of a desired component in the fluid sample very quickly. Using the described apparatus and methods, a fluid sample can be prepared, digitized, compartmentalized, assayed and the endpoint data collected in ˜30 minutes. The apparatus and methods can easily be adapted to provide parallel processing of a sample.

Abstract translation: 提出了一种用于分离，数字化和分析流体样品的数字分离（DS）芯片。 DS芯片包括流体层，其准备和分隔流体样品用于分析。 与井相邻的悬崖结构清除流体样品并防止可能干扰流体样品分析的颗粒进入孔。 在孔中进行脱脂流体样品分析，并且可以收集终点数据并且非常快速地用于确定流体样品中所需组分的原始浓度。 使用所述的装置和方法，可以制备流体样品，数字化，分区化，测定，并在〜30分钟内收集终点数据。 该装置和方法可以容易地适应于提供样品的并行处理。

公开(公告)号：US09145575B2

公开(公告)日：2015-09-29

申请号：US13752069

申请日：2013-01-28

Applicant: The Regents of the University of California

Inventor： Gang L. Liu ,  Jonathan A. Ellman ,  Luke P. Lee ,  Fanqing Frank Chen

IPC: A61K38/00 ,  C07K2/00 ,  C07K4/00 ,  C07K5/00 ,  C07K7/00 ,  C07K16/00 ,  C07K17/00 ,  C12Q1/37 ,  C12M3/00 ,  C40B30/08 ,  C40B40/10 ,  G01N33/574 ,  G01N33/58

CPC classification number: C12Q1/37 ,  G01N33/57434 ,  G01N33/587 ,  G01N2333/96433 ,  G01N2333/96466 ,  G01N2333/974

Abstract: This invention pertains to the in vitro detection of proteases using a single peptide-conjugate nanocrescent surface enhanced Raman scattering (SERS) probes with at least nanomolar sensitivity. The probe enables detection of proteolytic activity in extremely small volume and at low concentration. In certain embodiments the probes comprise an indicator for the detection of an active protease, where the indicator comprises a nanocrescent attached to a peptide, where said peptide comprises a recognition site for the protease and a Raman tag attached to the peptide.

Abstract translation: 本发明涉及使用具有至少纳摩尔灵敏度的单个肽 - 缀合的纳米新月面增强拉曼散射（SERS）探针的体外检测蛋白酶。 该探针能够以非常小的体积和低浓度检测蛋白水解活性。 在某些实施方案中，探针包括用于检测活性蛋白酶，其中所述指示符包括附连到的肽，其中所述肽包含用于蛋白酶和附连到该肽的拉曼标签的识别位点的nanocrescent的指示符。

公开(公告)号：US20150233894A1

公开(公告)日：2015-08-20

申请号：US14632021

申请日：2015-02-26

Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA

Inventor： Luke P. Lee ,  John R. Waldeisen ,  Ivan Dimov ,  Benjamin M. Ross

IPC: G01N33/49 ,  G01N33/86

CPC classification number: G01N33/4905 ,  A61B5/14535 ,  A61B5/14546 ,  A61B5/150022 ,  A61B5/150099 ,  A61B5/150358 ,  A61B5/15142 ,  A61B5/157 ,  G01N33/491 ,  G01N33/86

Abstract: A point-of-test diagnostic chip that is capable of simultaneous evaluation of blood coagulation time (INR Value) and hematocrit level. A finger prick volume of blood is placed at the inlet of the device, and the residual vacuum contained within the device provides a pressure gradient to drive the flow of whole blood. The INR value is determined by the distance the blood traverses down the channel before it coagulates. The channel is primed with tissue factor and phospholipids to induce coagulation. A control channel is primed with anticoagulants for use as a way to normalize initial loading times. The hematocrit level is determined by sedimentation of blood cells into a trench and the interface between blood cells that have overfilled the trench and plasma provide a visual readout.

Abstract translation: 能够同时评估血液凝固时间（INR Value）和血细胞比容水平的点测试诊断芯片。 手指的血液体积被放置在装置的入口处，并且包含在装置内的残余真空提供压力梯度以驱动全血的流动。 INR值由血液凝固前通过通道的距离决定。 该通道用组织因子和磷脂引发以诱导凝血。 控制通道用抗凝剂灌注，用作标准化初始加载时间的方式。 通过血细胞沉积到沟槽中确定血细胞比容水平，并且已经超过沟槽和血浆的血细胞之间的界面提供了视觉读数。