公开(公告)号：US20160268111A1

公开(公告)日：2016-09-15

申请号：US15026237

申请日：2014-10-07

Applicant: DH TECHNOLOGIES DEVELOPMENT PTE., LTD.

Inventor： Nic G. Bloomfield ,  Frank Londry

IPC: H01J49/00 ,  H01J49/42 ,  H01J49/40

CPC classification number: H01J49/0036 ,  H01J49/0027 ,  H01J49/004 ,  H01J49/061 ,  H01J49/40 ,  H01J49/4215 ,  H01J49/427

Abstract: A transmission window that has a constant rate of precursor ion transmission for each precursor ion is stepped across a mass range, producing a series of overlapping transmission windows across the mass range. The precursor ions produced at each step are fragmented. Resulting product ions are analyzed, producing a product ion spectrum for each step of the transmission window and a plurality of product ion spectra for the mass range. For at least one product ion of the plurality of product ion spectra, a function that describes how an intensity of the at least one product ion from the plurality of product ion spectra varies with precursor ion mass as the transmission window is stepped across the mass range is calculated. A precursor ion of the at least one product ion is identified from the function. An elution profile can also be determined from the function.

Abstract translation: 对于每个前体离子具有恒定速率的前体离子传输的传输窗口跨越质量范围，在质量范围内产生一系列重叠的传输窗口。 在每个步骤产生的前体离子是碎裂的。 分析得到的产物离子，产生透射窗的每个步骤的产物离子光谱和质量范围的多个产物离子光谱。 对于多个产物离子光谱中的至少一个产物离子，描述了当多个产物离子谱中的至少一个产物离子的强度随前驱离子质量而变化的函数，随着透射窗跨越质量范围 被计算。 根据该功能鉴定至少一种产物离子的前体离子。 也可以从功能中确定洗脱曲线。

公开(公告)号：US20160217988A1

公开(公告)日：2016-07-28

申请号：US15089529

申请日：2016-04-02

Applicant: DH Technologies Development Pte. Ltd.

Inventor： Nic G. Bloomfield ,  Frank Londry

IPC: H01J49/00 ,  H01J49/06

CPC classification number: H01J49/0036 ,  H01J49/0027 ,  H01J49/004 ,  H01J49/061 ,  H01J49/40 ,  H01J49/4215 ,  H01J49/427

Abstract: Ions are separated from a sample over time and filtered. The precursor ions produced at each step are fragmented. Resulting product ions are analyzed using a mass analyzer, producing a product ion spectrum for each step of the transmission window and a plurality of product ion spectra for the mass range for the each scan. The plurality of product ion spectra are received, producing a plurality of multi-scan product ion spectra. At least one product ion is selected from the plurality of multi-scan product ion spectra that is present at least two or more times in product ion spectra from each of two or more scans. A known separation profile of a precursor ion is fit to intensities from the at least one product ion in the plurality of multi-scan product ion spectra to reconstruct a separation profile of a precursor ion of the at least one product ion.

Abstract translation: 离子与样品随时间分离并过滤。 在每个步骤产生的前体离子是碎裂的。 使用质量分析仪分析所得产物离子，产生用于传输窗口的每个步骤的产物离子谱和用于每次扫描的质量范围的多个产物离子光谱。 接收多个产物离子光谱，产生多个多扫描产物离子光谱。 从多个多扫描产物离子光谱中选择至少一个产物离子，所述离子谱在两次或更多次扫描中的每一次的产物离子谱中至少存在两次或更多次。 前体离子的已知分离曲线适合于多个多扫描产物离子光谱中的至少一种产物离子的强度，以重建至少一种产物离子的前体离子的分离曲线。

公开(公告)号：US20160209361A1

公开(公告)日：2016-07-21

申请号：US14907450

申请日：2014-08-07

Applicant: DH TECHNOLOGIES DEVELOPMENT PTE. LTD.

Inventor： Nic G. Bloomfield ,  Alexandre V. Loboda ,  Igor Chernushevich

IPC: G01N27/62 ,  H01J49/40 ,  H01J49/42

CPC classification number: G01N27/62 ,  G16C20/20 ,  H01J49/0036 ,  H01J49/40 ,  H01J49/4225

Abstract: Systems and methods are provided for calculating and storing an average amplitude response for each peak of a mass spectrum during data acquisition. A mass analyzer is instructed to analyze N extractions of an ion beam, producing N sub-spectra. For each sub-spectrum of the N sub-spectra, a nonzero amplitude from an ADC detector subsystem is counted as one ion, producing a count of one for each ion. The ADC amplitudes and counts of the N sub-spectra are summed, producing a spectrum that includes a summed ADC amplitude and a total count for each ion. For each ion of the spectrum, an estimated ion count is calculated from a Poisson distribution of the total count of each ion for the N sub-spectra. For each ion of the spectrum, an average amplitude response is calculated by dividing the summed amplitude by the estimated ion count and stored.

Abstract translation: 提供了用于在数据采集期间计算和存储质谱的每个峰值的平均振幅响应的系统和方法。 指示质量分析仪分析离子束的N次提取，产生N个子光谱。 对于N个子光谱的每个子光谱，来自ADC检测器子系统的非零幅度被计为一个离子，为每个离子产生一个计数。 将N个子谱的ADC幅度和计数相加，产生包括ADC的幅度和每个离子的总计数的谱。 对于光谱的每个离子，从N个子光谱的每个离子的总计数的泊松分布计算估计的离子计数。 对于频谱的每个离子，通过将总和振幅除以估计的离子计数并存储来计算平均振幅响应。

公开(公告)号：US20160042933A1

公开(公告)日：2016-02-11

申请号：US14887215

申请日：2015-10-19

Applicant: DH Technologies Development PTE. LTD.

Inventor： Stephen A. Tate ,  Nic G. Bloomfield

IPC: H01J49/06 ,  H01J49/00

CPC classification number: H01J49/06 ,  H01J49/0031 ,  H01J49/004 ,  H01J49/429

Abstract: Systems and methods are used to band-pass filter ions from a mass range. A full spectrum is received for a full scan of a mass range using a tandem mass spectrometer. A mass selection window of the full spectrum is selected and a set of tuning parameter values is selected. The tandem mass spectrometer is instructed to perform a scan of the mass selection window using the set of tuning parameter values. A spectrum is received for the scan from the tandem mass spectrometer. A band-pass filtered spectrum is created for the mass range that includes values from the spectrum for the mass selection window of the mass range. Systems and methods are also used to band-pass filter ions from two or more mass selection windows across the mass range and to filter out ions from a mass selection window between two band-pass mass selection windows.

Abstract translation: 系统和方法用于将质量范围的滤波器离子带通。 接收到使用串联质谱仪进行质谱范围全扫描的全谱图。 选择全频谱的质量选择窗口，并选择一组调谐参数值。 指示串联质谱仪使用一组调谐参数值执行质量选择窗口的扫描。 接收来自串联质谱仪的扫描的光谱。 为包括质量范围的质量选择窗口的光谱值的质量范围创建带通滤波光谱。 系统和方法还用于对质谱范围内的两个或多个质量选择窗口进行带通滤波器离子，并从两个带通质量选择窗口之间的质量选择窗口滤出离子。