公开(公告)号：US20240003811A1

公开(公告)日：2024-01-04

申请号：US18335458

申请日：2023-06-15

Applicant: Quantum-Si Incorporated

Inventor： Gerard Schmid ,  Dajiang Yang ,  Eric A.G. Webster ,  Xin Wang ,  Todd Rearick ,  Changhoon Choi ,  Ali Kabiri ,  Kyle Preston ,  Brian Reed

IPC: G01N21/64 ,  H01L27/146

CPC classification number: G01N21/6454 ,  G01N21/6428 ,  H01L27/14603 ,  G01N21/6408 ,  G01N2021/6463

Abstract: Some aspects relate to an integrated circuit, comprising at least one photodetection region configured to generate charge carriers responsive to incident photons emitted from a sample, at least one charge storage region configured to receive the charge carriers from the photodetection region, and at least one controller configured to obtain information about the incident photons, the information comprising at least one member selected from the group comprising pulse duration and interpulse duration and at least one member selected from the group comprising wavelength information, luminescence lifetime information, and intensity information. In some embodiments, the information comprises at least three, four, and/or five members selected from the group comprising wavelength information, luminescence lifetime information, intensity information, pulse duration information, and interpulse duration information. In some embodiments, the information obtained may be used to identify the sample.

公开(公告)号：US20220128566A1

公开(公告)日：2022-04-28

申请号：US17511258

申请日：2021-10-26

Applicant: Quantum-Si Incorporated

Inventor： Thomas Christian ,  Kyle Preston ,  Brian Reed ,  Shannon Stewman

IPC: G01N33/58 ,  G01N21/64

Abstract: Aspects of the present disclosure relate to techniques for calibrating an integrated device. According to some embodiments, there is provided a method for calibrating an integrated device, the method comprising: exciting, with light from at least one excitation source, a reference dye molecule; obtaining a signal emitted by the reference dye molecule, the signal containing information representative of at least one characteristic of the reference dye molecule; and adjusting one or more subsequent measurements obtained from a sample based on the information obtained from the signal emitted by the reference dye molecule.

公开(公告)号：US11312944B2

公开(公告)日：2022-04-26

申请号：US15846967

申请日：2017-12-19

Applicant: Quantum-Si Incorporated

Inventor： Brian Reed ,  Mohammad Wadud Bhuiya ,  Manjula Pandey ,  Jeremy Lackey ,  Jonathan M. Rothberg ,  Thomas Christian

IPC: C12N9/12 ,  C12N15/52 ,  C12Q1/6869 ,  C12P19/34

Abstract: Compositions comprising modified recombinant polymerizing enzymes are provided, along with nucleic acid molecules encoding the modified polymerizing enzymes. In some aspects, methods of using such polymerizing enzymes to synthesize a nucleic acid molecule or to sequence a nucleic acid template are provided.

公开(公告)号：US20210395707A9

公开(公告)日：2021-12-23

申请号：US16914195

申请日：2020-06-26

Applicant: Quantum-Si Incorporated

Inventor： Brian Reed ,  Manjula Pandey

IPC: C12N9/12 ,  C12N11/00 ,  C12Q1/6806 ,  C12P19/34

Abstract: Compositions comprising modified recombinant polymerizing enzymes are provided, along with nucleic acid molecules encoding the modified polymerizing enzymes. In some aspects, methods of using such polymerizing enzymes to synthesize a nucleic acid molecule or to sequence a nucleic acid template are provided.

公开(公告)号：US20210147474A1

公开(公告)日：2021-05-20

申请号：US17082906

申请日：2020-10-28

Applicant: Quantum-Si Incorporated

Inventor： Matthew Dyer ,  Brian Reed

IPC: C07K1/02 ,  C07K1/04 ,  C07K1/113

Abstract: Aspects of the application provide methods of preparing a multiplexed sample for polypeptide sequencing, and compositions, kits and devices useful for the same.

公开(公告)号：US11001875B2

公开(公告)日：2021-05-11

申请号：US16212724

申请日：2018-12-07

Applicant: Quantum-Si Incorporated

Inventor： Jonathan M. Rothberg ,  Jeremy Lackey ,  Brian Reed ,  Xinghua Shi ,  Haidong Huang

IPC: C12Q1/6818 ,  C12Q1/6874 ,  G01N21/64 ,  C12Q1/6869

Abstract: Methods of sequencing molecules based on luminescence lifetimes and/or intensities are provided. In some aspects, methods of sequencing nucleic acids involve determining the luminescence lifetimes, and optionally luminescence intensities, of a series of luminescently labeled nucleotides incorporated during a nucleic acid sequencing reaction.

公开(公告)号：US10731209B2

公开(公告)日：2020-08-04

申请号：US15847001

申请日：2017-12-19

Applicant: Quantum-Si Incorporated

Inventor： James A. Ball ,  Jeremy Lackey ,  Brian Reed ,  Alexander Goryaynov ,  Jonathan M. Rothberg ,  Thomas Christian

IPC: C12Q1/6869 ,  C12Q1/6806 ,  B01L3/00 ,  B01L9/00 ,  C08L1/02 ,  C08L71/08

Abstract: Methods of loading a molecule of interest into a sample well are provided. In some aspects, methods of loading a molecule of interest into a sample well involve loading a molecule of interest into a sample well in the presence of a crowding agent and/or a condensing agent. In some aspects, methods of loading a sequencing template into a sample well are provided.

公开(公告)号：US20200209256A1

公开(公告)日：2020-07-02

申请号：US16709052

申请日：2019-12-10

Applicant: Quantum-Si Incorporated

Inventor： Brian Reed ,  Jeremy Lackey ,  Thomas Christian ,  Roger Nani ,  David Dodd ,  Kathren Croce ,  Alexander Goryaynov ,  Jonathan M. Rothberg

IPC: G01N33/68 ,  G01N33/58 ,  G16B30/00

Abstract: Aspects of the application provide methods of identifying and sequencing proteins, polypeptides, and amino acids, and compositions useful for the same. In some aspects, the application provides methods of obtaining data during a degradation process of a polypeptide, and outputting a sequence representative of the polypeptide. In some aspects, the application provides amino acid recognition molecules comprising a shielding element that enhances photostability in polypeptide sequencing reactions.

公开(公告)号：US20190330688A1

公开(公告)日：2019-10-31

申请号：US16212724

申请日：2018-12-07

Applicant: Quantum-Si Incorporated

Inventor： Jonathan M. Rothberg ,  Jeremy Lackey ,  Brian Reed ,  Xinghua Shi ,  Haidong Huang

IPC: C12Q1/6818 ,  C12Q1/6869 ,  G01N21/64 ,  C12Q1/6874

Abstract: Methods of sequencing molecules based on luminescence lifetimes and/or intensities are provided. In some aspects, methods of sequencing nucleic acids involve determining the luminescence lifetimes, and optionally luminescence intensities, of a series of luminescently labeled nucleotides incorporated during a nucleic acid sequencing reaction.

公开(公告)号：US20250130238A1

公开(公告)日：2025-04-24

申请号：US18752732

申请日：2024-06-24

Applicant: Quantum-Si Incorporated

Inventor： Brian Reed ,  Jeremy Lackey ,  Haidong Huang

IPC: G01N33/58 ,  C07K14/47 ,  C07K19/00 ,  C12Q1/6806 ,  G01N1/28 ,  G01N21/64 ,  G01N33/68 ,  G16B25/10 ,  G16B30/00 ,  G16B40/00 ,  G16B40/10 ,  G16B50/00 ,  G16B50/30

Abstract: Aspects of the application provide methods of identifying and sequencing proteins, polypeptides, and amino acids, and compositions useful for the same. In some aspects, the application provides methods of obtaining data during a degradation process of a polypeptide, and outputting a sequence representative of the polypeptide. In some aspects, the application provides amino acid recognition molecules comprising a shielding element that enhances photostability in polypeptide sequencing reactions.