公开(公告)号：US09233355B2

公开(公告)日：2016-01-12

申请号：US14400744

申请日：2013-05-23

Applicant: DAICEL CORPORATION

Inventor： Takafumi Onishi

IPC: B01J20/26 ,  B01J20/29 ,  B01J20/24 ,  B01J20/283 ,  B01J20/285 ,  B01J20/10 ,  B01J20/28 ,  C12Q1/68 ,  C12N15/09 ,  G01N30/88 ,  B01J20/286 ,  B01J20/32 ,  C07K1/16

CPC classification number: B01J20/29 ,  B01J20/103 ,  B01J20/24 ,  B01J20/26 ,  B01J20/28016 ,  B01J20/28083 ,  B01J20/283 ,  B01J20/285 ,  B01J20/286 ,  B01J20/327 ,  B01J20/3272 ,  B01J20/3274 ,  B01J2220/54 ,  C07K1/16 ,  C12N15/09 ,  C12Q1/68 ,  G01N30/88

Abstract: Provided is a novel separating agent obtained by using core/shell particles as a support and fixing any of various ligands to the support by physical adsorption. The separating agent contains a support and a ligand fixed to the surface of the support by physical adsorption, and is characterized in that the support is core/shell particles each formed of a core constituted of a nonporous inorganic substance and a porous shell, the shell having a pore diameter of 30 nm or larger, the shell being constituted of a hydrolyzate of a polyalkoxysiloxane, and the ligand is an optically active polymer, an optically inactive polyester, a protein, or a nucleic acid.

Abstract translation: 提供了通过使用核/壳颗粒作为载体获得的新型分离剂，并通过物理吸附将各种配体中的任何一种固定在载体上。 分离剂含有通过物理吸附固定在载体表面的载体和配体，其特征在于载体是由无孔无机物质和多孔壳构成的核心形成的核/壳颗粒，壳体 孔径为30nm以上，壳由聚烷氧基硅氧烷的水解物构成，配体为光学活性聚合物，光学活性聚酯，蛋白质或核酸。

公开(公告)号：US20150353702A1

公开(公告)日：2015-12-10

申请号：US14762475

申请日：2014-09-19

Applicant: ISTANBUL TEKNIK UNIVERSITESI

Inventor： Omer Suat TASKIN ,  Baris KISKAN ,  Yusuf YAGCI ,  Abdullah AKSU ,  Nuray BALKIS

IPC: C08J9/28 ,  B01J20/285 ,  B01J20/30 ,  B01J20/26

CPC classification number: C08J9/286 ,  B01J20/22 ,  B01J20/262 ,  B01J20/264 ,  B01J20/282 ,  B01J20/285 ,  B01J20/30 ,  B01J20/3071 ,  B01J20/3085 ,  B01J20/3092 ,  B01J2220/54 ,  B01J2220/58 ,  C08J2361/20

Abstract: The present invention relates to a column filling material which is filled inside the columns used in chromatographic analysis of aromatic hydrocarbons, alkaloids, flavonoids and pesticides and provides high adsorption by means of its porous structure, and a production method thereof essentially comprising the steps of dissolving melamine and anthraquinone in a solvent (11), carrying out the reacting by refluxing the solution (12), washing the material obtained after reflux (13), drying the washed material by freezing (14).

Abstract translation: 本发明涉及填充在用于芳烃，生物碱，类黄酮和农药的色谱分析中的柱内的柱填充材料，并且通过其多孔结构提供高吸附性，其制备方法基本上包括以下步骤：将 三聚氰胺和蒽醌在溶剂（11）中，通过回流溶液（12）进行反应，洗涤回流后获得的材料（13），通过冷冻干燥洗涤的材料（14）。

公开(公告)号：US20150300998A1

公开(公告)日：2015-10-22

申请号：US14477060

申请日：2014-09-04

Applicant: The Board of Trustees of the University of Illinois

Inventor： Kenneth S. Suslick ,  Jacqueline M. Rankin

IPC: B01J20/281 ,  C08L63/00 ,  C08L83/04 ,  G01N30/60

CPC classification number: G01N30/48 ,  B01J20/28097 ,  B01J20/285 ,  B01J2220/86 ,  C08L63/00 ,  C08L83/04 ,  G01N30/6095 ,  G01N2030/486

Abstract: A microcolumn for use in gas chromatography comprises a self-supporting polymer body that functions as a stationary phase and a structural support. The polymer body comprises an enclosed channel having a length L, height h and width w extending therethrough and one or more channel walls surrounding the enclosed channel. The one or more channel walls are integrally formed with the polymer body. The polymer body and the one or more channel walls may comprise a phase-separated polymer composition.

Abstract translation: 用于气相色谱的微柱包括用作固定相和结构载体的自支撑聚合物体。 聚合物主体包括封闭的通道，其具有延伸穿过其中的长度L，高度h和宽度w以及围绕封闭通道的一个或多个通道壁。 一个或多个通道壁与聚合物主体一体地形成。 聚合物体和一个或多个通道壁可以包含相分离的聚合物组合物。

公开(公告)号：US20150258540A1

公开(公告)日：2015-09-17

申请号：US14682456

申请日：2015-04-09

Applicant: EMD Millipore Corporation

Inventor： David Yavorsky ,  John Amara ,  Joaquin Umana ,  William Cataldo ,  Mikhail Kozlov ,  Matthew Stone

IPC: B01J39/26 ,  C07K1/18 ,  C12N7/00 ,  B01D15/36 ,  C07K16/00

CPC classification number: B01J39/26 ,  B01D15/327 ,  B01D15/361 ,  B01D15/362 ,  B01D15/363 ,  B01D15/3804 ,  B01J20/28023 ,  B01J20/28059 ,  B01J20/285 ,  B01J20/287 ,  B01J20/3244 ,  B01J41/20 ,  B01J47/014 ,  B01J47/127 ,  C07K1/18 ,  C07K16/00 ,  C12N7/00 ,  C12N7/02 ,  G01N2030/521 ,  Y10S502/52724 ,  Y10T428/2938 ,  Y10T428/2973

Abstract: Adsorptive media for chromatography, particularly ion-exchange chromatography, derived from a shaped fiber. In certain embodiments, the functionalized shaped fiber presents a fibrillated or ridged structure which greatly increases the surface area of the fibers when compared to ordinary fibers. Also disclosed herein is a method to add surface pendant functional groups that provides cation-exchange or anion-exchange functionality to the high surface area fibers. This pendant functionality is useful for the ion-exchange chromatographic purification of biomolecules, such as monoclonal antibodies (mAbs).

Abstract translation: 来自成形纤维的用于色谱的吸附介质，特别是离子交换层析。 在某些实施方案中，与普通纤维相比，官能化成形纤维呈现原纤化或脊状结构，其大大增加了纤维的表面积。 本文还公开了向高表面积纤维添加提供阳离子交换或阴离子交换官能团的表面侧基官能团的方法。 这种侧链官能团可用于生物分子的离子交换色谱纯化，如单克隆抗体（mAb）。

公开(公告)号：US09114383B2

公开(公告)日：2015-08-25

申请号：US10920922

申请日：2004-08-17

Applicant: Douglas T. Gjerde ,  Ronald Jones ,  Allen Burge ,  Christopher P. Hanna ,  Leon S. Yengoyan

Inventor： Douglas T. Gjerde ,  Ronald Jones ,  Allen Burge ,  Christopher P. Hanna ,  Leon S. Yengoyan

IPC: G01N30/02 ,  G01N30/56 ,  B01J20/285 ,  B01J20/286 ,  B01L3/02 ,  B82Y30/00 ,  B01D15/22 ,  G01N1/40 ,  G01N35/10

CPC classification number: B01J20/285 ,  B01D15/22 ,  B01J20/286 ,  B01J2220/54 ,  B01J2220/58 ,  B01J2220/64 ,  B01L3/0275 ,  B01L2200/0631 ,  B82Y30/00 ,  G01N1/405 ,  G01N30/02 ,  G01N30/56 ,  G01N2035/1053 ,  B01D15/3804

Abstract: The invention provides extraction columns for the purification of an analyte (e.g., a biological macromolecule, such as a peptide, protein or nucleic acid) from a sample solution, as well as methods for making and using such columns. The columns typically include a bed of extraction media positioned in the column between two frits. In some embodiments, the extraction columns employ modified pipette tips as column bodies. In some embodiments, the invention provides methods of storing columns in a wet state.

Abstract translation: 本发明提供了用于从样品溶液中纯化分析物（例如生物大分子，如肽，蛋白质或核酸）的提取柱，以及制备和使用这些色谱柱的方法。 柱通常包括位于两个玻璃料之间的柱中的提取介质床。 在一些实施方案中，提取柱使用改进的移液管尖作为柱体。 在一些实施方案中，本发明提供了在湿状态下储存塔的方法。

公开(公告)号：US20140343263A1

公开(公告)日：2014-11-20

申请号：US14287602

申请日：2014-05-27

Applicant: Langtech International Pty., Limited

Inventor： Tim Lang ,  Wayne Simpkins

IPC: C07H1/06 ,  C07H17/07 ,  C07H17/04

CPC classification number: C07H1/06 ,  B01D15/1864 ,  B01D15/361 ,  B01D15/363 ,  B01J20/285 ,  C07H17/04 ,  C07H17/07

Abstract: The invention relates to a process for separating bioactive compounds obtained from vegetable materials. The invention also relates to a process for extracting bioactive compounds from vegetable material.

Abstract translation: 本发明涉及分离从植物材料获得的生物活性化合物的方法。 本发明还涉及从植物材料中提取生物活性化合物的方法。

公开(公告)号：US20140329995A1

公开(公告)日：2014-11-06

申请号：US14358821

申请日：2012-11-28

Applicant: GE HEALTHCARE BIO-SCIENCES AB

Inventor： Hans J. Johansson ,  Ronnie Palmgren

IPC: C07K1/22 ,  C07K14/31 ,  C07K14/245

CPC classification number: C07K1/22 ,  B01D15/34 ,  B01D15/3809 ,  B01J20/285 ,  B01J20/286 ,  B01J20/289 ,  B01J20/3274 ,  C07K14/245 ,  C07K14/31 ,  C07K2319/00

Abstract: The invention discloses a polypeptide capable of binding immunoglobulins or immunoglobulin-containing proteins, which polypeptide comprises six or more domains of protein Z or the C domain of protein A or a functional variant thereof. It also discloses separation matrices comprising the polypeptide and methods of using the separation matrices for separation of immunoglobulins or immunoglobulin-containing proteins.

Abstract translation: 本发明公开了能够结合免疫球蛋白或含有免疫球蛋白的蛋白质的多肽，该多肽包含蛋白质Z的六个或更多个结构域或蛋白质A的C结构域或其功能变体。 还公开了包含多肽的分离基质和使用分离基质分离免疫球蛋白或含有免疫球蛋白的蛋白质的方法。

公开(公告)号：US20140329919A1

公开(公告)日：2014-11-06

申请号：US14188385

申请日：2014-02-24

Applicant: Waters Technologies Corporation

Inventor： Kevin D. Wyndham ,  John E. O'Gara

IPC: C08J9/36

CPC classification number: C08J9/36 ,  B01J20/28095 ,  B01J20/285 ,  B01J20/3057 ,  B01J2220/46 ,  B29C67/20 ,  C08G83/001 ,  Y10T428/249953 ,  Y10T428/24996 ,  Y10T428/249967 ,  Y10T428/249969 ,  Y10T428/249975 ,  Y10T428/249981

Abstract: Porous hybrid inorganic/organic materials comprising ordered domains are disclosed. Methods of making the materials and use of the materials for chromatographic are also disclosed.

Abstract translation: 公开了包含有序域的多孔杂化无机/有机材料。 还公开了制备材料和用于色谱的材料的使用方法。

公开(公告)号：US20140326649A1

公开(公告)日：2014-11-06

申请号：US14275250

申请日：2014-05-12

Applicant: WATERS TECHNOLOGIES CORPORATION

Inventor： John E. O'Gara ,  Julia Ding ,  Daniel B. Walsh

IPC: B01J20/285 ,  B05D7/22 ,  C08G77/20 ,  B01J20/26 ,  B01D15/20 ,  B01D15/22

CPC classification number: B01D15/265 ,  B01D15/206 ,  B01D15/22 ,  B01J20/26 ,  B01J20/285 ,  B01J2220/82 ,  B01J2220/86 ,  B05D7/22 ,  B82Y30/00 ,  C08G77/20 ,  C08J5/005 ,  Y10T428/26 ,  Y10T428/31612 ,  Y10T428/31663

Abstract: Novel materials for chromatographic separations, processes for their preparation, and separation devices containing the chromatographic materials. In particular, hybrid inorganic/organic monolith materials comprising a polymerized scaffolding nanocomposite (PSN), wherein the nanocomposite contains a scaffolding functionally capable of chemically interacting with a surface of a second material are described. The hybrid inorganic/organic materials have enhanced wall adhesion and increased resistance to shrinkage as compared to prior art monolith materials. The improved adhesion of the monoliths enable the preparation of capillary columns with an internal diameter (I.D.)≧50 μm.

Abstract translation: 用于色谱分离的新型材料，其制备方法和含有色谱材料的分离装置。 特别地，描述了包含聚合的支架纳米复合材料（PSN）的混合无机/有机整料材料，其中纳米复合材料包含功能上能够与第二材料的表面化学相互作用的支架。 混合无机/有机材料与现有技术的整料材料相比具有增强的壁粘合性和增加的抗收缩性。 整体的改进的附着力使得能够制备内径（I.D.）≥50μm的毛细管柱。

公开(公告)号：US08877477B2

公开(公告)日：2014-11-04

申请号：US13062960

申请日：2009-09-22

Applicant: Brad William Woonton ,  Milton Thomas William Hearn ,  Pankaj Maharjan ,  Kirthi De Silva ,  William Roy Jackson

Inventor： Brad William Woonton ,  Milton Thomas William Hearn ,  Pankaj Maharjan ,  Kirthi De Silva ,  William Roy Jackson

IPC: C09H3/02 ,  C09H3/00 ,  C09H1/04 ,  C08H1/00 ,  B01J20/26 ,  C08F2/44 ,  B01D15/38 ,  C08F20/56 ,  C08F226/00 ,  B01D15/36 ,  B01J39/26 ,  C07K1/18 ,  C08J3/12 ,  C08J3/24 ,  C08F26/06 ,  B01J20/285 ,  C08F220/56 ,  C08F222/38 ,  C08F220/06

CPC classification number: C08L51/02 ,  B01D15/362 ,  B01D15/3876 ,  B01J20/264 ,  B01J20/267 ,  B01J20/285 ,  B01J39/19 ,  B01J39/26 ,  C07K1/18 ,  C08F2/44 ,  C08F20/56 ,  C08F26/06 ,  C08F220/06 ,  C08F220/56 ,  C08F222/385 ,  C08F226/00 ,  C08H1/00 ,  C08J3/12 ,  C08J3/24 ,  C08J2333/26 ,  C08L1/08 ,  C08L5/00 ,  C08L29/02 ,  C08L51/003 ,  C08L2555/80

Abstract: The present invention relates to a method for isolating proteins from a solution containing the proteins. The invention also relates to a method for the chromatographic separation of proteins. The present invention also relates to crosslinked hydroxylic polymer particles functionalized with temperature-responsive copolymer, and to methods of preparing such particles.

Abstract translation: 本发明涉及从含有蛋白质的溶液中分离蛋白质的方法。 本发明还涉及一种色谱分离蛋白质的方法。 本发明还涉及用温度响应共聚物官能化的交联羟基聚合物颗粒，以及制备这种颗粒的方法。