公开(公告)号：US08329453B2

公开(公告)日：2012-12-11

申请号：US13191120

申请日：2011-07-26

Applicant: C. Frederick Battrell ,  Troy D. Daiber ,  William Samuel Hunter

Inventor： C. Frederick Battrell ,  Troy D. Daiber ,  William Samuel Hunter

IPC: C12M1/34

CPC classification number: G01N21/6456 ,  B01L7/52 ,  C12M23/16 ,  G01N21/645 ,  G01N21/6452 ,  G01N21/6454 ,  G01N2201/0245

Abstract: Disclosed is a compact, microprocessor-controlled instrument for fluorometric assays in liquid samples, the instrument having a floating stage with docking bay for receiving a microfluidic cartridge and a scanning detector head with on-board embedded microprocessor for controlling source LEDs, emission signal amplification and filtering in an isolated, low noise, high gain environment within the detector head. Multiple optical channels may be incorporated in the scanning head. In a preferred configuration, the assay is validated using dual channel optics for monitoring a first fluorophore associated with a target analyte and a second fluorophore associated with a control. Applications include molecular biological assays based on PCR amplification of target nucleic acids and fluorometric assays in general, many of which require temperature control during detection. Sensitivity and resistance to bubble interference during scanning are shown to be improved by use of a heating block with reflective mirror face in intimate contact with a thermo-optical window enclosing the liquid sample.

Abstract translation: 公开了一种用于液体样品中的荧光测定的紧凑型微处理器控制仪器，该仪器具有带有用于接收微流控盒的对接槽的浮动台和具有用于控制源LED的板上嵌入式微处理器的扫描检测器头，发射信号放大和 在检测器头内的隔离，低噪声，高增益环境中进行滤波。 多个光通道可并入扫描头。 在优选的配置中，使用双通道光学器件验证测定，用于监测与靶分析物相关联的第一荧光团和与对照相关联的第二荧光团。 应用包括基于目标核酸的PCR扩增和荧光测定的分子生物测定法，其中许多在检测期间需要温度控制。 通过使用具有与包围液体样品的热光学窗口紧密接触的反射镜面的加热块，可以改善扫描期间对气泡干扰的灵敏度和抗性。

公开(公告)号：US08300218B2

公开(公告)日：2012-10-30

申请号：US13355401

申请日：2012-01-20

Applicant: Michael D. Furtaw ,  Robert D. Eckles ,  Georgiy G. Burba ,  Dayle K. McDermitt ,  Jonathan M. Welles

Inventor： Michael D. Furtaw ,  Robert D. Eckles ,  Georgiy G. Burba ,  Dayle K. McDermitt ,  Jonathan M. Welles

IPC: G01N21/00

CPC classification number: G01N21/3504 ,  G01K13/02 ,  G01K2013/024 ,  G01N1/2247 ,  G01N21/05 ,  G01N2201/0245

Abstract: Gas analyzer systems and methods for measuring concentrations of gasses and in particular dry mole fraction of components of a gas. The systems and method allow for rapid measurement of the gas density and/or dry mole fraction of gases for a number of environmental monitoring applications, including high speed flux measurements. A novel coupling design allows for tool-free removal of a cell enclosing a flow path to enable in field cleaning of optical components.

Abstract translation: 气体分析仪系统和测量气体浓度的方法，特别是气体组分的干摩尔分数。 这些系统和方法允许快速测量多种环境监测应用的气体密度和/或气体的干摩尔分数，包括高速通量测量。 新的耦合设计允许无需移除封闭流动通道的电池，从而可以对光学部件进行现场清洁。

公开(公告)号：US20120075623A1

公开(公告)日：2012-03-29

申请号：US13241960

申请日：2011-09-23

Applicant: Daisuke Matsumoto ,  Yasunori Shiraki

Inventor： Daisuke Matsumoto ,  Yasunori Shiraki

IPC: G01J1/00

CPC classification number: G01N27/44721 ,  B01L3/502715 ,  B01L9/527 ,  B01L2300/0654 ,  G01N21/0303 ,  G01N21/05 ,  G01N2021/0346 ,  G01N2201/0245

Abstract: An analyzing apparatus includes a microchip, a detecting unit and an analyzing-measuring unit. The microchip is formed of a light transmissive material formed with a separation fluid channel that is a light measuring part. The detecting unit includes an emitted-light guiding unit that emits light to the separation fluid channel, and a received-light guiding unit that receives light through the separation fluid channel. The emitted-light guiding unit or the received-light guiding unit placed at a position facing a microchip support table via the microchip abuts the microchip, and pushes the microchip in a direction toward the microchip support table. The analyzing-measuring unit includes the detecting unit, the emitted-light guiding unit and the received-light guiding unit, and detects a constituent of a sample filled in the separation fluid channel.

Abstract translation: 分析装置包括微芯片，检测单元和分析测量单元。 微芯片由形成有作为光测​​量部分的分离流体通道形成的透光材料形成。 检测单元包括向分离流体通道发射光的发射光引导单元和通过分离流体通道接收光的接收光引导单元。 经由微芯片放置在面向微芯片支撑台的位置处的发射光引导单元或接收光引导单元抵靠微芯片，并且朝向微芯片支撑台的方向推动微芯片。 分析测量单元包括检测单元，发射光引导单元和接收光引导单元，并且检测填充在分离流体通道中的样品的成分。

公开(公告)号：US07410793B2

公开(公告)日：2008-08-12

申请号：US10440920

申请日：2003-05-19

Applicant: Steven J. Boege ,  Howard G. King ,  Eugene F. Young ,  Johannes P. Sluis ,  Mark F. Oldham

Inventor： Steven J. Boege ,  Howard G. King ,  Eugene F. Young ,  Johannes P. Sluis ,  Mark F. Oldham

IPC: C12M1/34 ,  C12M3/00 ,  C12M1/00 ,  G01N21/64 ,  G01N21/66

CPC classification number: G01N21/6486 ,  B01L7/52 ,  C12Q2545/101 ,  G01N21/6452 ,  G01N2021/6419 ,  G01N2021/6421 ,  G01N2021/6439 ,  G01N2021/6441 ,  G01N2021/6463 ,  G01N2021/6482 ,  G01N2201/0245 ,  G01N2201/0628 ,  G01N2201/0635 ,  Y10S435/808 ,  Y10S435/809

Abstract: An optical instrument is provided for simultaneously illuminating two or more spaced-apart reaction regions with excitation beams generated by a light source. The light source can include an area light array of light emitting diodes, one or more solid state lasers, one or more micro-wire lasers, or a combination thereof. According to various embodiments, a Fresnel lens can be disposed along a beam bath between the light source and the reaction regions. Methods of analysis using the optical instrument are also provided.

Abstract translation: 提供了一种光学仪器，用于同时用由光源产生的激发光束照射两个或更多个间隔开的反应区域。 光源可以包括发光二极管的区域光阵列，一个或多个固态激光器，一个或多个微线激光器，或其组合。 根据各种实施例，菲涅耳透镜可以沿着光源和反应区域之间的光束槽设置。 还提供了使用光学仪器的分析方法。

公开(公告)号：US20070238161A1

公开(公告)日：2007-10-11

申请号：US11804151

申请日：2007-05-17

Applicant: Anthony Cerrone ,  Edward Lakatos ,  Michael Gambini ,  Eugene Young ,  Susan Stone ,  Judith Atwood

Inventor： Anthony Cerrone ,  Edward Lakatos ,  Michael Gambini ,  Eugene Young ,  Susan Stone ,  Judith Atwood

IPC: C12M1/34 ,  C12M1/36

CPC classification number: G01N21/6428 ,  B01L3/5085 ,  B01L7/52 ,  B01L2200/10 ,  B01L2300/0654 ,  B01L2300/0663 ,  B01L2300/1811 ,  C12Q1/686 ,  C12Q2545/101 ,  G01N21/0303 ,  G01N21/274 ,  G01N21/645 ,  G01N21/6452 ,  G01N2021/6419 ,  G01N2021/6421 ,  G01N2021/6439 ,  G01N2021/6441 ,  G01N2021/6463 ,  G01N2021/6482 ,  G01N2201/0245 ,  G01N2201/06113 ,  G01N2201/0628 ,  G01N2201/0635 ,  G01N2201/0636 ,  G01N2201/068 ,  C12Q2563/107

Abstract: An optical instrument monitors PCR replication of DNA in a reaction apparatus having a temperature cycled block with vials of reaction ingredients including dye that fluoresces in presence of double-stranded DNA. A beam splitter passes an excitation beam to the vials to fluoresce the dye. An emission beam from the dye is passed by the beam splitter to a CCD detector from which a processor computes DNA concentration. A reference strip with a plurality of reference emitters emit reference beams of different intensity, from which the processor selects an optimum emitter for compensating for drift. Exposure time is automatically adjusted for keeping within optimum dynamic ranges of the CCD and processor. A module of the beam splitter and associated optical filters is associated with selected dye, and is replaceable for different dyes.

公开(公告)号：US20050190366A1

公开(公告)日：2005-09-01

申请号：US10929320

申请日：2004-08-30

Applicant: Steven Boege ,  Mark Oldham ,  Liana Ilkova

Inventor： Steven Boege ,  Mark Oldham ,  Liana Ilkova

IPC: G01N21/25 ,  G01N21/64

CPC classification number: G01N21/6452 ,  G01N21/253 ,  G01N2021/6439 ,  G01N2021/6471 ,  G01N2021/6476 ,  G01N2021/6478 ,  G01N2201/0245

Abstract: A fluorometry device and method adapted to determine concentration of spectrally distinguishable species in a biological sample with a plurality of movable optical devices.

Abstract translation: 一种荧光测定装置和方法，其适于用多个可移动光学装置确定生物样品中光谱可区分物质的浓度。

公开(公告)号：US20040009586A1

公开(公告)日：2004-01-15

申请号：US10456196

申请日：2003-06-06

Inventor： Mark F. Oldham ,  Eugene F. Young

IPC: C12M001/34

CPC classification number: G01N21/6486 ,  B01L3/5085 ,  B01L7/52 ,  C12Q2545/101 ,  G01N21/274 ,  G01N21/6428 ,  G01N21/645 ,  G01N21/6452 ,  G01N2021/6419 ,  G01N2021/6421 ,  G01N2021/6439 ,  G01N2021/6441 ,  G01N2021/6463 ,  G01N2021/6482 ,  G01N2201/0245 ,  G01N2201/0628 ,  G01N2201/0635 ,  Y10S435/808 ,  Y10S435/809

Abstract: An instrument is provided that can monitor nucleic acid sequence amplification reactions, for example, PCR amplification of DNA and DNA fragments. The instrument includes a multi-notch filter disposed along one or both of an excitation beam path and an emission beam path. Methods are also provided for monitoring nucleic acid sequence amplifications using an instrument that includes a multi-notch filter disposed along a beam path.

Abstract translation: 提供了可以监测核酸序列扩增反应的仪器，例如DNA和DNA片段的PCR扩增。 仪器包括沿激发光束路径和发射光束路径中的一个或两个设置的多陷波滤波器。 还提供了使用包括沿着光束路径设置的多陷波滤波器的仪器来监测核酸序列扩增的方法。

公开(公告)号：US20190011351A1

公开(公告)日：2019-01-10

申请号：US16015349

申请日：2018-06-22

Applicant: OTSUKA ELECTRONICS CO., LTD.

Inventor： Yusuke IZUTANI ,  Ikuo WAKAYAMA

IPC: G01N15/14 ,  G01B11/08

CPC classification number: G01N15/1434 ,  G01B11/08 ,  G01N15/0211 ,  G01N21/51 ,  G01N21/53 ,  G01N2015/03 ,  G01N2015/1493 ,  G01N2021/015 ,  G01N2201/024 ,  G01N2201/0245

Abstract: An optical measurement apparatus includes a main body base, an optical base movably combined with the main body base, a measurement optical system fixed to the optical base, and an optical base moving mechanism which moves the optical base relative to the main body base. The optical base moving mechanism moves the optical base relative to the main body base between an internal measurement position and an external measurement position. A measurement object position of the measurement optical system coincides with an internal measurement object position within the main body base. The measurement object position of the measurement optical system coincides with an external measurement object position outside the main body base.

公开(公告)号：US20180275058A1

公开(公告)日：2018-09-27

申请号：US15756938

申请日：2016-09-02

Applicant: SeLux Diagnostics, Inc.

Inventor： Eric STERN ,  Aleksandar VACIC ,  Nathan B. PURMORT

IPC: G01N21/64 ,  B01L3/00 ,  G01J3/44 ,  G01N21/69 ,  G01N21/76

CPC classification number: G01N21/6428 ,  B01L3/5027 ,  B01L3/502715 ,  B01L2200/0605 ,  B01L2300/0681 ,  B01L2300/0816 ,  B01L2300/0867 ,  B01L2300/0887 ,  B01L2400/0487 ,  B01L2400/06 ,  B01L2400/0688 ,  B82Y15/00 ,  G01J3/44 ,  G01J3/4406 ,  G01N21/03 ,  G01N21/645 ,  G01N21/6452 ,  G01N21/6489 ,  G01N21/69 ,  G01N21/76 ,  G01N2021/0321 ,  G01N2021/0328 ,  G01N2021/0346 ,  G01N2021/6482 ,  G01N2201/0245

Abstract: In some aspects, reader systems for optically detecting binding agents or analyte complexes in a sample as a result of performing biochemical assays can include: a housing defining a positioning receptacle to receive the sample; an excitation source to generate incident light directed at the sample; at least one solid-state photomultiplier detector configured to: i) receive a light emitted by at least one label associated with the binding agents and/or analyte complexes within the sample; and ii) produce a signal in response to receiving the light emitted by the at least one label or substrate solution that is physically or chemically modified by the said label, the at least one detector being connected to integrated signal processing electronics to process the signal; and a user interface in communication with the signal processing electronics for conveying one or more results of the one or more biochemical as says.

公开(公告)号：US20150118690A1

公开(公告)日：2015-04-30

申请号：US14396123

申请日：2013-04-23

Applicant: Siemens Healthcare Diagnostics Inc.

Inventor： Jeffrey R. Jasperse ,  Normand P. Desmarais

IPC: G01N21/76 ,  G01N21/64

CPC classification number: G01N21/76 ,  G01N21/645 ,  G01N2021/6463 ,  G01N2021/6471 ,  G01N2021/6476 ,  G01N2201/0245 ,  G01N2201/0634 ,  G01N2201/0636 ,  G01N2201/0666

Abstract: A sample analyzer has an illuminator for illuminating an assay sample to cause luminescence, and a support for a sample vessel containing the assay sample. The support is adapted to position the assay sample proximate the illuminator. A detector is positioned along an optical axis extending from the illuminator, through the positioned assay sample, to the detector, so as to detect the luminescence from the assay sample. A reflector is removably disposed between the illuminator and the assay sample so as to reflect a portion of the luminescence back through the positioned assay sample toward the detector.

Abstract translation: 样品分析仪具有用于照射测定样品以产生发光的照明器，以及含有测定样品的样品容器的支架。 该支架适于将测定样品定位在靠近照明器处。 检测器沿着从照明器延伸的光轴定位，通过定位的测定样品到检测器，以便检测来自测定样品的发光。 反射器可拆卸地设置在照明器和测定样品之间，以便通过定位的测定样品将一部分发光反射回检测器。