公开(公告)号：US20180229192A1

公开(公告)日：2018-08-16

申请号：US15954779

申请日：2018-04-17

Applicant: EMD Millipore Corporation

Inventor： Martin Morrissey ,  Brian Pereira

IPC: B01F7/00 ,  B01F15/00 ,  B01F13/08 ,  B01F7/16

CPC classification number: B01F7/00241 ,  B01F7/162 ,  B01F7/1635 ,  B01F13/0827 ,  B01F13/0872 ,  B01F15/0085

Abstract: A disposable container, such as a deformable bag, for a fluid, having one or more inlets and one or more outlets and an impeller assembly within the container to cause mixing, dispersing, homogenizing and/or circulation of one or more ingredients contained or added to the container. The impeller assembly has a protective hood surrounding at least a portion of the moveable blades or vanes of the impeller assembly and being above at least a portion of the blades or vanes. The hood surrounds the blades or vanes and arcs over the height of the blades or vanes. The hood is shaped in a dome shape or semi-spherical shape that is around and above the impeller blades and acts as a protector for the container surface against the impeller assembly both during shipping and storage as well as when in use, particularly at lower liquid levels.

公开(公告)号：US20180208624A1

公开(公告)日：2018-07-26

申请号：US15920995

申请日：2018-03-14

Applicant: EMD Millipore Corporation

Inventor： Mikhail Kozlov ,  William Cataldo ,  Jeffrey Caron

IPC: C07K1/34 ,  C08F259/08 ,  C08J5/22 ,  B01D15/10 ,  B01D37/00 ,  B01J20/288 ,  B01J20/289 ,  C07K1/18 ,  C07K16/00 ,  C08F220/18 ,  C08F220/54 ,  B01J20/26 ,  C08F220/58 ,  C08F220/56 ,  B01J20/32 ,  B01J39/26

CPC classification number: C07K1/34 ,  B01D15/10 ,  B01D37/00 ,  B01J20/267 ,  B01J20/288 ,  B01J20/289 ,  B01J20/321 ,  B01J20/3212 ,  B01J20/327 ,  B01J20/3276 ,  B01J20/3291 ,  B01J39/26 ,  B01J2220/80 ,  C07K1/18 ,  C07K16/00 ,  C08F220/18 ,  C08F220/54 ,  C08F220/56 ,  C08F220/58 ,  C08F259/08 ,  C08F2220/1825 ,  C08F2220/1858 ,  C08J5/2243 ,  C08J2351/00

Abstract: Novel compositions for removing impurities such as, protein aggregates, from a sample containing a protein of interest, e.g., an antibody. Such compositions can be used prior to the virus filtration step during protein purification, to remove aggregates and protect the virus filter from fouling, therefore improving virus filter capacity. A porous solid support including a co-polymer having at least two monomers, wherein at least one of the monomers comprises acrylamide and at least a second monomer comprises a hydrophobic binding group, where the solid support selectively binds protein aggregates, thereby to separate the monomeric protein of interest from the protein aggregates. The method can be performed under neutral to high pH and high conductivity conditions.

公开(公告)号：US09975095B2

公开(公告)日：2018-05-22

申请号：US14440373

申请日：2013-11-05

Applicant: EMD Millipore Corporation

Inventor： Martin Morrissey ,  Brian Pereira

IPC: B01F7/00 ,  B01F7/16 ,  B01F13/08 ,  B01F15/00

CPC classification number: B01F7/00241 ,  B01F7/162 ,  B01F7/1635 ,  B01F13/0827 ,  B01F13/0872 ,  B01F15/0085

Abstract: A disposable container, such as a deformable bag, for a fluid, having one or more inlets and one or more outlets and an impeller assembly within the container to cause mixing, dispersing, homogenizing and/or circulation of one or more ingredients contained or added to the container. The impeller assembly has a protective hood surrounding at least a portion of the moveable blades or vanes of the impeller assembly and being above at least a portion of the blades or vanes. The hood surrounds the blades or vanes and arcs over the height of the blades or vanes. The hood is shaped in a dome shape or semi-spherical shape that is around and above the impeller blades and acts as a protector for the container surface against the impeller assembly both during shipping and storage as well as when in use, particularly at lower liquid levels.

公开(公告)号：US20180133453A1

公开(公告)日：2018-05-17

申请号：US15867915

申请日：2018-01-11

Applicant: EMD Millipore Corporation

Inventor： James Vigna ,  Joseph Muldoon ,  John Cieciuch ,  Robert Langlois

IPC: A61M39/18 ,  F16L37/367 ,  F16L55/07 ,  F16L37/36

Abstract: Fluid transfer device and method that allows for sterile wet connections, allows the connection to be reversed, and allows the connection to be reconnected, while leaving the connectors sterile and reusable under pressure. In certain embodiments, the device includes a first member and a second member, the latter adapted to receive the former in locking engagement upon actuation of the device to create fluid communication between the two in a sterile manner. Each of the members include a door that when opened, allows the first member to be linearly displaced into the second member to allow fluid to be transferred. When fluid transfer is complete, the first member can be retracted from the second member, and the doors closed.

公开(公告)号：US20180085743A1

公开(公告)日：2018-03-29

申请号：US15677526

申请日：2017-08-15

Applicant: EMD Millipore Corporation

Inventor： David Yavorsky ,  John Amara ,  Joaquin Umana ,  William Cataldo ,  Mikhail Kozlov ,  Matthew Stone

IPC: B01J39/26 ,  B01D15/36 ,  B01D15/38 ,  B01J20/285 ,  B01J20/287 ,  B01J20/28 ,  B01J20/32 ,  B01J41/20 ,  B01J47/014 ,  B01J47/127 ,  C07K1/18 ,  C07K16/00 ,  C12N7/00 ,  C12N7/02 ,  B01D15/32

CPC classification number: B01J39/26 ,  B01D15/327 ,  B01D15/361 ,  B01D15/362 ,  B01D15/363 ,  B01D15/3804 ,  B01J20/28023 ,  B01J20/28059 ,  B01J20/285 ,  B01J20/287 ,  B01J20/3244 ,  B01J41/20 ,  B01J47/014 ,  B01J47/127 ,  C07K1/18 ,  C07K16/00 ,  C12N7/00 ,  C12N7/02 ,  G01N2030/521 ,  Y10S502/52724 ,  Y10T428/2938 ,  Y10T428/2973

Abstract: Adsorptive media for chromatography, particularly ion-exchange chromatography, derived from a shaped fiber. In certain embodiments, the functionalized shaped fiber presents a fibrillated or ridged structure which greatly increases the surface area of the fibers when compared to ordinary fibers. Also disclosed herein is a method to add surface pendant functional groups that provides cation-exchange or anion-exchange functionality to the high surface area fibers. This pendant functionality is useful for the ion-exchange chromatographic purification of biomolecules, such as monoclonal antibodies (mAbs).

公开(公告)号：US20180078903A1

公开(公告)日：2018-03-22

申请号：US15562171

申请日：2016-06-01

Applicant: EMD Millipore Corporation

Inventor： Brian Hillier

IPC: B01D65/00 ,  B01D63/08

Abstract: A sealing case for a filtration cassette that reliably prevents leaks after assembly and installation into a filtration system. The sealing case fits on the filtration cassette and provides a fluid-tight seal between the sealing case and around at least one port in the filtration cassette when installed in a filtration system. The sealing case may comprise two halves that mate together, and each half may be identical. The internal surface of the sealing case is flat or substantially flat.

公开(公告)号：US09901729B2

公开(公告)日：2018-02-27

申请号：US14773426

申请日：2014-03-26

Applicant: EMD Millipore Corporation

Inventor： James Vigna ,  Joseph Muldoon ,  John Cieciuch ,  Robert Langlois

IPC: F16L37/36 ,  A61M39/18 ,  F16L37/367 ,  F16L55/07

CPC classification number: A61M39/18 ,  F16L37/36 ,  F16L37/367 ,  F16L55/07 ,  F16L2201/44

Abstract: Fluid transfer device and method that allows for sterile wet connections, allows the connection to be reversed, and allows the connection to be reconnected, while leaving the connectors sterile and reusable under pressure. In certain embodiments, the device includes a first member and a second member, the latter adapted to receive the former in locking engagement upon actuation of the device to create fluid communication between the two in a sterile manner. Each of the members include a door that when opened, allows the first member to be linearly displaced into the second member to allow fluid to be transferred. When fluid transfer is complete, the first member can be retracted from the second member, and the doors closed.

公开(公告)号：US09891218B2

公开(公告)日：2018-02-13

申请号：US15006153

申请日：2016-01-26

Applicant: EMD Millipore Corporation

Inventor： Chris Scott ,  Phillip Clark ,  Kurt E. Greenizen ,  Ryan A. Amara ,  Robert Colonna

IPC: G01N21/75 ,  G01N33/558 ,  G01N33/543 ,  B01L3/00 ,  B01L9/00 ,  G01N33/569 ,  B01J20/28

CPC classification number: G01N33/558 ,  B01J20/28033 ,  B01J20/28035 ,  B01J20/2804 ,  B01L3/5023 ,  B01L9/52 ,  B01L2200/025 ,  B01L2300/0609 ,  B01L2300/0681 ,  B01L2300/069 ,  B01L2300/0809 ,  B01L2300/12 ,  B01L2300/126 ,  B01L2400/0487 ,  B01L2400/049 ,  G01N33/54366 ,  G01N33/56911 ,  G01N2469/10 ,  G01N2469/20

Abstract: The embodiments disclosed herein are directed to an apparatus useful in conducting detection of compounds on blotting membranes. The device is comprised of several layers including a porous support layer below the blotting membrane(s), a flow distributor above the blotting membrane(s) and optionally a well on the flow distributor to contain the liquid to the desired area and to allow for lower starting volumes of such liquid. Preferably, the flow distributor is a non-binding or low binding hydrophilic porous membrane such as a 0.22 micron membrane and the support layer is a grid or sintered porous material. The distributor and support are held together to form an envelope around the membrane(s). The use of a hinge, clips and other such devices is preferred in doing so.

公开(公告)号：US09861982B2

公开(公告)日：2018-01-09

申请号：US15006765

申请日：2016-01-26

Applicant: EMD Millipore Corporation

Inventor： Terry Gaige ,  Andrew Zayac ,  Paul Sydlowski ,  Philip Lee

IPC: B01L99/00 ,  B01L3/00 ,  C12M3/06 ,  C12M1/00 ,  G01N35/00

CPC classification number: B01L3/50273 ,  B01L3/502715 ,  B01L3/563 ,  B01L2200/025 ,  B01L2200/027 ,  B01L2200/0689 ,  B01L2300/0681 ,  B01L2300/0861 ,  B01L2300/14 ,  B01L2300/165 ,  B01L2400/049 ,  C12M23/16 ,  C12M23/40 ,  G01N35/00871

Abstract: In one embodiment, a removable pneumatic connector, comprises a body having a plurality of bores passing through, each bore surrounded by a sealing member on an inner surface of the body. A plurality of gas lines may be placed within a corresponding bore. A vacuum port is disposed on the inner surface of the body, and an outer seal on the inner surface of the body surrounds the sealing members and the vacuum port. A vacuum line may be placed within the vacuum port, and configured to deliver negative pressure to the vacuum port. A vacuum holding area is created in the volume between the outer seal and each of the sealing members when the inner surface of the body is placed against a substrate. When the vacuum line is activated, a vacuum is created within the vacuum holding area, creating a positive seal between the body and the substrate.

公开(公告)号：US20170298091A1

公开(公告)日：2017-10-19

申请号：US15521428

申请日：2015-09-30

Applicant: EMD Millipore Corporation

Inventor： Matthew T. Stone ,  John P. Amara

IPC: C07K1/20 ,  B01D63/02 ,  B01D15/38 ,  C07K1/36 ,  C07K1/18

CPC classification number: C07K1/20 ,  B01D15/3804 ,  B01D63/02 ,  B01D2311/2626 ,  C07K1/18 ,  C07K1/36

Abstract: The present invention refers to new species of an ion exchange adsorber which is suitable for the separation of host cell proteins (HCPs), antibody fragments and low molecular weight substances from solutions containing antibodies. The invention especially refers to a process for purifying biological samples by separating biomolecules of interest and impurities, comprising steps of contacting a sample with said chromatography media consisting of fibers, said fibers having imparted thereon functionality enabling ion exchange chromatography and/or hydrophobic interaction.