公开(公告)号：US20170267995A1

公开(公告)日：2017-09-21

申请号：US15611527

申请日：2017-06-01

Applicant: Roche Molecular Systems, Inc.

Inventor： Geza Burghardt ,  Willem Mulder ,  Rolf Schneebeli

IPC: C12N15/10 ,  B01F11/00 ,  B01F15/00 ,  B03C1/01 ,  B03C1/28 ,  G01N1/40 ,  B01L3/00 ,  B01L3/02 ,  B01L7/00

CPC classification number: C12N15/1006 ,  B01F11/0022 ,  B01F15/00733 ,  B01F15/00876 ,  B01L3/021 ,  B01L3/50 ,  B01L3/508 ,  B01L7/52 ,  B01L2200/0647 ,  B01L2200/141 ,  B01L2300/044 ,  B01L2300/0672 ,  B01L2300/0829 ,  B01L2300/0832 ,  B01L2400/043 ,  B01L2400/0457 ,  B03C1/01 ,  B03C1/288 ,  B03C2201/26 ,  G01N1/405

Abstract: A device, method and system is provided for binding particles for the separation and/or isolation of biological materials. In particular, a container is provided including a suspension of binding particles for the isolation of biological material, inner walls forming an elongate groove at the bottom of said container, and a cover having openings and/or being penetrable for a linear arrangement of multiple pipets or pipet tips, said openings being located above and parallel to said elongate groove.

公开(公告)号：US20170266670A1

公开(公告)日：2017-09-21

申请号：US15479537

申请日：2017-04-05

Applicant: Advantageous Systems, LLC

Inventor： Adam L. STEIN

IPC: B03C1/00 ,  B01J20/28 ,  B01J20/32 ,  B01J20/34 ,  B03C1/01 ,  B03C1/015 ,  B03C1/28 ,  B82Y30/00 ,  C02F1/48 ,  C02F1/68 ,  C08K3/22 ,  C08K5/1545 ,  H01F1/00 ,  C02F1/26 ,  C02F101/12 ,  C02F103/08

CPC classification number: B03C1/00 ,  B01J20/28007 ,  B01J20/28009 ,  B01J20/3204 ,  B01J20/321 ,  B01J20/3212 ,  B01J20/3238 ,  B01J20/324 ,  B01J20/345 ,  B01J20/3475 ,  B03C1/01 ,  B03C1/015 ,  B03C1/288 ,  B03C1/30 ,  B03C2201/18 ,  B03C2201/26 ,  B82Y30/00 ,  C02F1/265 ,  C02F1/288 ,  C02F1/488 ,  C02F1/683 ,  C02F2101/10 ,  C02F2101/106 ,  C02F2101/12 ,  C02F2103/08 ,  C02F2303/04 ,  C02F2305/04 ,  C02F2305/08 ,  C08K3/22 ,  C08K5/1545 ,  C08K2003/2265 ,  C08K2201/01 ,  C08K2201/011 ,  H01F1/0054 ,  Y02W10/37

Abstract: Disclosed are magnetic nanoparticles and methods of using magnetic nanoparticles for selectively removing biologics, small molecules, analytes, ions, or other molecules of interest from liquids.

公开(公告)号：US09764030B2

公开(公告)日：2017-09-19

申请号：US14404469

申请日：2013-06-11

Applicant: NANO3D BIOSCIENCES INC.

Inventor： Glauco R. Souza

IPC: C12N5/00 ,  A61K41/00 ,  A61N2/00 ,  A61L27/18 ,  A61L27/38 ,  A61L27/50 ,  G01N33/543 ,  G01N33/58 ,  B03C1/01 ,  B03C1/034 ,  B03C1/30

CPC classification number: A61K41/0052 ,  A61L27/18 ,  A61L27/38 ,  A61L27/50 ,  A61L2400/12 ,  A61L2430/20 ,  A61L2430/30 ,  A61N2/00 ,  B03C1/01 ,  B03C1/034 ,  B03C1/30 ,  B03C2201/26 ,  C12N5/0062 ,  C12N5/0068 ,  C12N2529/00 ,  G01N33/54346 ,  G01N33/587

Abstract: Methods of making and using a magnetic ECM are disclosed. The ECM comprises positively and negatively charged nanoparticles, wherein one of said nanoparticles contains a magnetically responsive element. When the magnetic ECM is seeded with cells, the cells will be magnetized and can be levitated for 3-D cell culture.

公开(公告)号：US09752968B2

公开(公告)日：2017-09-05

申请号：US14132823

申请日：2013-12-18

Applicant: LUMINEX CORPORATION

Inventor： Eric Smith ,  Adam Schilffarth

IPC: G01N1/00 ,  G01N1/40 ,  B01L3/00 ,  B03C1/033 ,  B03C1/28

CPC classification number: G01N1/4044 ,  B01L3/5085 ,  B01L2200/0668 ,  B01L2300/0829 ,  B01L2300/0851 ,  B03C1/0332 ,  B03C1/288 ,  B03C2201/18 ,  B03C2201/26 ,  Y10T436/25375

Abstract: Systems configured to isolate particles in a fluid assay are disclosed. Methods for collecting a sample of magnetic particles from a liquid are also disclosed. In certain embodiments, the disclosed systems and methods include a magnetic actuator coupled to a chassis, where the magnetic actuator comprises at least one shielded rotatable magnet.

公开(公告)号：US20170234781A1

公开(公告)日：2017-08-17

申请号：US15586132

申请日：2017-05-03

Applicant: Accelerate Diagnostics, Inc.

Inventor： Landon Prisbrey ,  Elise Blackmore ,  Kenneth R. Hance ,  Steven W. Metzger ,  Kevin Marshall

IPC: G01N1/40 ,  C12N13/00 ,  B03C5/00

CPC classification number: G01N1/40 ,  B03C5/005 ,  B03C2201/26 ,  C12M47/12 ,  C12N7/00 ,  C12N13/00 ,  C12N2710/00051 ,  C12Q1/02 ,  C12Q1/24 ,  G01N1/34 ,  G01N33/491 ,  G01N2001/4038 ,  G02B21/367

Abstract: Methods and systems for purifying one or more microbial cells and/or viruses from a biological sample are provided. The biological sample is added to a well disposed in a medium. A potential is applied across the medium to cause the contaminants to enter one or more walls of the well, and retain the microbial cells and/or viruses in the well. The microbial cells and/or viruses can be removed from the well, and optionally adhered or fixed to a surface, or detected. In one embodiment, the microbial cells and/or viruses are retained in the well by embedding in the medium. The medium including the embedded microbial cells and/or viruses may be excised or otherwise removed and transferred to a glass slide or other solid surface. In some examples, a biological sample containing contaminants and one or more microbial cells is introduced to a well disposed in a porous filter medium, wherein the porous filter medium includes pores smaller than the one or more microbial cells, thereby preventing the one or more microbial cells from entering the porous filter medium.

公开(公告)号：US20170183722A1

公开(公告)日：2017-06-29

申请号：US15415044

申请日：2017-01-25

Applicant: Raindance Technologies, Inc.

Inventor： Darren Roy Link

IPC: C12Q1/68 ,  B01L3/00

CPC classification number: C12Q1/6848 ,  B01F3/0807 ,  B01F13/0062 ,  B01J19/0093 ,  B01J2219/00783 ,  B01J2219/00788 ,  B01J2219/00828 ,  B01J2219/00831 ,  B01J2219/00833 ,  B01J2219/00837 ,  B01J2219/00853 ,  B01J2219/0086 ,  B01J2219/00869 ,  B01J2219/00889 ,  B01J2219/00903 ,  B01J2219/0097 ,  B01J2219/00988 ,  B01L3/502715 ,  B01L3/502746 ,  B01L3/502753 ,  B01L3/502776 ,  B01L3/502784 ,  B01L3/565 ,  B01L7/525 ,  B01L9/527 ,  B01L2200/027 ,  B01L2200/0636 ,  B01L2200/0647 ,  B01L2200/0673 ,  B01L2200/10 ,  B01L2200/141 ,  B01L2300/0636 ,  B01L2300/0816 ,  B01L2300/0861 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2300/165 ,  B01L2400/0415 ,  B01L2400/0424 ,  B01L2400/0487 ,  B01L2400/086 ,  B03C5/005 ,  B03C5/026 ,  B03C2201/26 ,  C12N15/1075 ,  C12Q1/00 ,  C12Q1/6806 ,  C12Q1/6827 ,  C12Q1/6844 ,  C12Q1/686 ,  C12Q1/6874 ,  C12Q2525/191 ,  C12Q2563/159 ,  C12Q2565/628 ,  C12Q2565/629 ,  G01N1/38 ,  G01N15/147 ,  G01N35/08 ,  G01N2035/00158 ,  G01N2035/00237 ,  G01N2035/00326 ,  C12Q2535/101 ,  C12Q2563/149

Abstract: The invention generally relates to assemblies for displacing droplets from a vessel that facilitate the collection and transfer of the droplets while minimizing sample loss. In certain aspects, the assembly includes at least one droplet formation module, in which the module is configured to form droplets surrounded by an immiscible fluid. The assembly also includes at least one chamber including an outlet, in which the chamber is configured to receive droplets and an immiscible fluid, and in which the outlet is configured to receive substantially only droplets. The assembly further includes a channel, configured such that the droplet formation module and the chamber are in fluid communication with each other via the channel. In other aspects, the assembly includes a plurality of hollow members, in which the hollow members are channels and in which the members are configured to interact with a vessel. The plurality of hollow members includes a first member configured to expel a fluid immiscible with droplets in the vessel and a second member configured to substantially only droplets from the vessel. The assembly also includes a main channel, in which the second member is in fluid communication with the main channel. The assembly also includes at least one analysis module connected to the main channel.

公开(公告)号：US09617145B2

公开(公告)日：2017-04-11

申请号：US14520510

申请日：2014-10-22

Applicant: Berkeley Lights, Inc.

Inventor： Keith J. Breinlinger ,  Daniele Malleo ,  Gaetan L. Mathieu ,  J. Tanner Nevill ,  Alexander H. Slocum ,  Mark P. White

IPC: B03C7/02 ,  B81B7/02 ,  B03C5/00 ,  B03C5/02 ,  G01N33/00 ,  G01N27/447 ,  B01L3/00 ,  G01N33/50

CPC classification number: B81B7/02 ,  B01L3/502761 ,  B01L2200/027 ,  B01L2200/0652 ,  B01L2200/0668 ,  B01L2300/044 ,  B01L2300/0636 ,  B01L2300/0645 ,  B01L2300/0816 ,  B01L2300/0819 ,  B01L2300/0829 ,  B01L2300/0851 ,  B01L2300/0864 ,  B01L2300/087 ,  B01L2300/0877 ,  B01L2400/0424 ,  B01L2400/0433 ,  B01L2400/0454 ,  B01L2400/086 ,  B03C5/005 ,  B03C5/026 ,  B03C7/023 ,  B03C2201/26 ,  C12M23/16 ,  C12M41/46 ,  C12M47/02 ,  G01N27/447 ,  G01N27/44756 ,  G01N27/44791 ,  G01N33/5023 ,  G01N33/543 ,  G01N33/54326 ,  G01N35/10

Abstract: A group of micro-objects in a holding pen in a micro-fluidic device can be selected and moved to a staging area, from which the micro-objects can be exported from the micro-fluidic device. The micro-fluidic device can have a plurality of holding pens, and each holding pen can isolate micro-objects located in the holding pen from micro-objects located in the other holding pens or elsewhere in the micro-fluidic device. The selected group of micro-objects can comprise one or more biological cells, such as a clonal population of cells. Embodiments of the invention can thus select a particular group of clonal cells in a micro-fluidic device, move the clonal cells to a staging area, and export the clonal cells from the micro-fluidic device while maintaining the clonal nature of the exported group.

公开(公告)号：US09605253B2

公开(公告)日：2017-03-28

申请号：US14154797

申请日：2014-01-14

Applicant: Centre National de la Recherche Scientifique

Inventor： Joel Voldman ,  Thibault Honegger ,  David Peyrade

IPC: C12N13/00 ,  A61N1/20 ,  A61N1/32 ,  B03C5/00 ,  C12M3/00 ,  C12M1/00 ,  C12M1/42 ,  C12N5/0793 ,  B03C5/02 ,  B01L3/00

CPC classification number: C12N13/00 ,  A61N1/205 ,  A61N1/326 ,  B01L3/5027 ,  B03C5/005 ,  B03C5/026 ,  B03C2201/26 ,  C12M21/08 ,  C12M23/34 ,  C12M35/02 ,  C12M35/04 ,  C12N5/0619 ,  C12N2529/00 ,  C12N2533/54 ,  C12N2535/00

Abstract: Systems and methods for altering neurite growth are generally described. In some embodiments, a system may include a neuron comprising a neurite and electrodes able to generate a physical guidance cue. The physical guidance cue may be used to alter the growth of the neurite and may be temporally and spatially dynamic, such that neurite growth may be altered in a spatial and/or temporal manner. Dynamic control of neurite growth may be used to form directional neural connections, intersections, and/or overlaps.

公开(公告)号：US20170072395A1

公开(公告)日：2017-03-16

申请号：US15293062

申请日：2016-10-13

Applicant: Biological Dynamics, Inc.

Inventor： Rajaram KRISHNAN ,  David CHARLOT ,  Eugene TU ,  James MCCANNA ,  Lucas KUMOSA ,  Paul SWANSON ,  Robert TURNER ,  Kai YANG ,  Irina DOBROVOLSKAYA ,  David LIU

IPC: B01L3/00 ,  C12Q1/68 ,  B03C7/02 ,  B03C5/00 ,  B03C5/02 ,  C12N15/10 ,  B01L7/00

CPC classification number: B01L3/502715 ,  B01D57/02 ,  B01L7/52 ,  B01L2300/0636 ,  B01L2300/0645 ,  B01L2400/0424 ,  B03C5/005 ,  B03C5/026 ,  B03C7/023 ,  B03C2201/26 ,  C12N15/1003 ,  C12N15/101 ,  C12Q1/6806 ,  G01N27/447 ,  G01N27/44704 ,  G01S5/163 ,  G01S11/12 ,  G06F3/0346

Abstract: The present invention includes methods, devices and systems for isolating a nucleic acid from a fluid comprising cells. In various aspects, the methods, devices and systems may allow for a rapid procedure that requires a minimal amount of material and/or results in high purity nucleic acid isolated from complex fluids such as blood or environmental samples.

Abstract translation: 本发明包括用于从包含细胞的流体分离核酸的方法，装置和系统。 在各个方面，方法，装置和系统可以允许需要最少量的材料和/或产生从诸如血液或环境样品的复杂流体分离的高纯度核酸的快速程序。

公开(公告)号：US20170071535A1

公开(公告)日：2017-03-16

申请号：US15342262

申请日：2016-11-03

Applicant: ARkival Technology Corp.

Inventor： Ronald D. Weiss

IPC: A61B5/00 ,  B03C1/025 ,  A61K49/18 ,  B03C1/30 ,  A61B5/05 ,  A61B5/055

CPC classification number: A61B5/4839 ,  A61B5/0035 ,  A61B5/0515 ,  A61B5/055 ,  A61B5/7246 ,  A61B2562/0285 ,  A61K49/0002 ,  A61K49/1818 ,  B03C1/025 ,  B03C1/30 ,  B03C2201/18 ,  B03C2201/26

Abstract: The invention features an apparatus for producing a fluid stream having plurality of nanoparticles in the fluid stream. The apparatus includes a source configured to provide a fluid stream having a first randomly sized distribution of a plurality of nanoparticles; a flow control zone configured to receive the fluid stream from the source and to control the fluid stream to produce a controlled fluid stream having a selected flow rate; a separation zone configured to receive and to separate the selectively controlled fluid stream into at least one separated fluid stream having a non-randomly sized distribution of nanoparticles; and a collection zone capable of receiving the separated fluid stream according to at least one non-random sized distribution of nanoparticles to produce at least one collected stream. The apparatus is configured for a continuous flow of the fluid stream. A size of a nanoparticle can be related to an intrinsic core diameter, a hydrodynamic diameter, and a combination of intrinsic core diameter and hydrodynamic diameter measurements. The nanoparticles can include non-magnetic nanoparticles, partially magnetic nanoparticles, magnetic nanoparticles, superparamagnetic nanoparticles, and a combination of at least two different nanoparticle types. The invention also features methods for producing said fluid streams. The invention further features apparatus and methods for cancer confirmation and targeted therapeutic drug development.

Abstract translation: 本发明的特征在于一种用于在流体流中产生具有多个纳米颗粒的流体流的装置。 该装置包括：源，其配置成提供具有多个纳米颗粒的第一随机大小分布的流体流; 流动控制区域，其被配置为从所述源接收流体流并且控制所述流体流以产生具有选定流量的受控流体流; 分离区，被配置为接收并且将选择性控制的流体流分离成具有非随机大小的纳米颗粒分布的至少一个分离的流体流; 以及能够根据至少一个非随机大小的纳米颗粒分布接收分离的流体流以产生至少一个收集的流的收集区。 该装置构造成用于流体流的连续流动。 纳米颗粒的尺寸可以与本征芯直径，流体动力学直径以及本征芯直径和流体动力学直径测量的组合有关。 纳米颗粒可以包括非磁性纳米颗粒，部分磁性纳米颗粒，磁性纳米颗粒，超顺磁性纳米颗粒，以及至​​少两种不同纳米颗粒类型的组合。 本发明还涉及生产所述流体流的方法。 本发明还涉及用于癌症确认和靶向治疗药物开发的装置和方法。