公开(公告)号：US09676816B2

公开(公告)日：2017-06-13

申请号：US14378230

申请日：2014-01-21

Applicant: THE BOARD OF TRUSTEES OF THE UNIVERSITY OF ARKANSAS

Inventor： Suresh Kumar Thallapuranam ,  Srinivas Jayanthi ,  Jacqueline Morris ,  Alicia Brown ,  David McNabb ,  Ralph Henry

IPC: C12N9/64 ,  C07K14/00 ,  C07K1/22 ,  B01D15/20 ,  B01D15/22 ,  B01D15/38 ,  B01D69/08 ,  B01J20/28 ,  B01J20/286

CPC classification number: C07K1/22 ,  B01D15/206 ,  B01D15/22 ,  B01D15/3809 ,  B01D69/08 ,  B01J20/28078 ,  B01J20/286 ,  C07K14/00 ,  C07K2319/20 ,  C07K2319/50 ,  C12N9/6424 ,  C12Y304/21043

Abstract: In one aspect, affinity tags for recombinant protein purification are described herein which, in some embodiments, can mitigate or overcome disadvantages of prior affinity tag systems. In some embodiments, for example, affinity tags described herein permit efficient elution of desired recombinant proteins with simplified solution systems, such as alkali metal salt solutions. An affinity tag described herein comprises an amino acid sequence including a repeating amino acid unit of BXXXBXX, wherein B is an amino acid selected from the group consisting of histidine, lysine and arginine and X is an amino acid selected from the group consisting of amino acids other than histidine, lysine and arginine.

公开(公告)号：US09663559B2

公开(公告)日：2017-05-30

申请号：US15063471

申请日：2016-03-07

Applicant: GE Healthcare BioProcess R&D AB

Inventor： Martin Hall ,  Sture Larsson ,  Andreas Muranyi ,  Gustav Rodrigo ,  Jinyu Zou ,  Per-Mikael Aberg

IPC: C07K14/31 ,  B01D15/38 ,  B01J20/286 ,  C07K1/22 ,  B01J20/32 ,  C07K16/00 ,  C07K17/00

CPC classification number: B01J20/24 ,  B01D15/3809 ,  B01J20/28019 ,  B01J20/285 ,  B01J20/286 ,  B01J20/289 ,  B01J20/3212 ,  B01J20/3219 ,  B01J20/3274 ,  B01J20/3293 ,  B01J2220/52 ,  B01J2220/54 ,  C07K1/22 ,  C07K14/31 ,  C07K16/00 ,  C07K17/00 ,  C07K17/10 ,  C07K2317/55

Abstract: The present invention relates to a chromatography ligand, which comprises Domain C from Staphylococcus protein A (SpA), or a functional fragment or variant thereof. The chromatography ligand presents an advantageous capability of withstanding harsh cleaning in place (CIP) conditions, and is capable of binding Fab fragments of antibodies. The ligand may be provided with a terminal coupling group, such as arginine or cysteine, to facilitate its coupling to an insoluble carrier such as beads or a membrane. The invention also relates to a process of using the ligand in isolation of antibodies, and to a purification protocol which may include washing steps and/or regeneration with alkali.

公开(公告)号：US20170106349A1

公开(公告)日：2017-04-20

申请号：US14820670

申请日：2015-08-07

Applicant: INSTRACTION GMBH

Inventor： Klaus GOTTSCHALL ,  Markus ARENDT ,  Andres KIRSCHFELD ,  Christian MEYER ,  Markus WEIS ,  Martin WELTER ,  Lothar ZISER

IPC: B01J20/286 ,  C07K1/22 ,  B01D15/38 ,  B01J20/32

CPC classification number: B01J20/286 ,  B01D15/3804 ,  B01J20/3242 ,  B01J20/3255 ,  B01J20/327 ,  B01J20/3272 ,  B01J20/3282 ,  B01J20/3285 ,  C07K1/22

Abstract: Sorbent comprising a solid support material, the surface of which comprises first residues comprising a binuclear heteroaromatic structure comprising besides carbon atoms at least one of the heteroatoms N, O, S, and second residues comprising a mononuclear heteroaromatic structure comprising besides carbon atoms at least one of the heteroatoms N, O, S.

公开(公告)号：US09574017B2

公开(公告)日：2017-02-21

申请号：US13871239

申请日：2013-04-26

Applicant: MDC MAX-DELBRUCK-CENTRUM FUR MOLEKULARE MEDIZIN BERLIN-BUCH ,  FRESENIUS MEDICAL CARE DEUTSCHLAND GMBH

Inventor： Rudolf Kunze ,  Gerd Wallukat ,  Peter Rosenthal ,  Richard Straube

IPC: C07K17/08 ,  A61K38/08 ,  C07K14/72 ,  C07K16/28 ,  B01J20/286 ,  A61K38/17 ,  B01J20/32

CPC classification number: C07K17/08 ,  A61K38/08 ,  A61K38/1787 ,  B01J20/286 ,  B01J20/3251 ,  C07K14/723 ,  C07K16/28 ,  C07K2317/34

Abstract: A peptide having an ED50 of less than 500 nm, in particular 10 nM to an antibody which recognizes an epitope on an extracellular α1 loop 2 or β2 loop1 of a human adrenoceptor wherein the antibody's binding to the epitope results in an increase of γ-secretase activity, an increased release of β-amyolid molecules and/or cellular dysfunction of cerebral blood vessel cells, glia cells or neurons, wherein the ED50 value is measured by bioassay.

Abstract translation: 具有小于500nm的ED 50，特别是10nM识别人肾上腺素受体的细胞外α1环2或β2环1上的表位的抗体的肽，其中抗体与表位的结合导致γ-分泌酶的增加 活性，增加β型固体分子的释放和/或脑血管细胞，神经胶质细胞或神经元的细胞功能障碍，其中通过生物测定来测量ED 50值。

公开(公告)号：US09573973B2

公开(公告)日：2017-02-21

申请号：US14396207

申请日：2013-04-22

Applicant: GE HEALTHCARE BIOPROCESS R&D AB

Inventor： Jesper Hansson ,  Gustav Rodrigo ,  Tobias E Soderman

IPC: B01D15/36 ,  C07K1/22 ,  B01D15/18 ,  B01J20/286 ,  B01J20/32 ,  B01D15/38 ,  B01J20/26 ,  B01J20/28 ,  B01J20/289 ,  B01J20/30 ,  C07H1/08 ,  G01N30/88

CPC classification number: B01J39/26 ,  B01D15/1864 ,  B01D15/34 ,  B01D15/361 ,  B01D15/362 ,  B01D15/3809 ,  B01J20/24 ,  B01J20/261 ,  B01J20/265 ,  B01J20/267 ,  B01J20/28014 ,  B01J20/28033 ,  B01J20/286 ,  B01J20/289 ,  B01J20/3085 ,  B01J20/3212 ,  B01J20/3278 ,  B01J20/3282 ,  B01J2220/80 ,  C07H1/08 ,  C07K1/22 ,  G01N2030/8827 ,  Y02P20/582

Abstract: The invention discloses a method of separating a biomolecule from at least one other component in a liquid, comprising a step of contacting said liquid with a separation matrix comprising a solid support and polymer chains bound to said solid support. The polymer chains comprise units derived from a first monomer of structure CH2═CH-L-X, where L is a covalent bond or an alkyl ether or hydroxysubstituted alkyl ether chain comprising 2-6 carbon atoms, and X is a sulfonate or phosphonate group.

Abstract translation: 本发明公开了一种从液体中的至少一种其它组分分离生物分子的方法，包括使所述液体与包含固体支持物和与所述固体支持物结合的聚合物链的分离基质接触的步骤。 聚合物链包含衍生自结构为CH 2 = CH-L-X的第一单体的单元，其中L为共价键或包含2-6个碳原子的烷基醚或羟基取代的烷基醚链，X为磺酸盐或膦酸酯基团。

公开(公告)号：US09527059B2

公开(公告)日：2016-12-27

申请号：US14660153

申请日：2015-03-17

Applicant: THE FLORIDA INTERNATIONAL UNIVERSITY BOARD OF TRUSTEES

Inventor： Abuzar Kabir ,  Kenneth G. Furton

IPC: B01L3/00 ,  B01L99/00 ,  B01J20/32 ,  B01J20/286 ,  G01N1/40 ,  G01N1/02

CPC classification number: B01J20/3212 ,  B01J20/286 ,  B01J20/3204 ,  B01J20/321 ,  B01J20/3276 ,  B01J20/328 ,  B01J20/3285 ,  B01J2220/64 ,  G01N1/405 ,  G01N2001/027

Abstract: A field kit for collecting analytical samples has one or more dynamic fabric phase sorptive extraction (DFPSE) devices and/or fabric phase sorptive extraction (FPSE) devices and a plurality of containers for storing and transporting the DFPSEs and/or FPSEs that were used for sampling. The field kit has media for documenting information concerning the site, quantity, date, and/or other pertinent information concerning the sampling. Samples can be maintained within the kit for any required period of storage. Sampling can be done once or a plurality of times, such that an initial analysis can be carried out and analysis can be repeated using a portion of a FPSE or with a redundant FPSE that has been stored. The DFPSE device is a sampling device including a plurality of FPSEs, such that a number of different types of analytes can be sampled in different layers of the DFPSE. At least one external surface layer of the DFPSE is a barrier FPSE that restricts solids from underlying layers.

Abstract translation: 用于收集分析样品的现场试剂盒具有一个或多个动态织物相吸附萃取（DFPSE）装置和/或织物相吸附提取（FPSE）装置和用于储存和运输用于DFPSE和/或FPSE的DFPSE和/或FPSE的多个容器 采样。 现场工具包有媒体记录有关采样的站点，数量，日期和/或其他相关信息的信息。 样品可以保存在试剂盒中以保持任何所需的储存期。 可以进行一次或多次采样，使得可以执行初始分析并且可以使用FPSE的一部分或已经存储的冗余FPSE来重复分析。 DFPSE设备是包括多个FPSE的采样设备，使得可以在DFPSE的不同层中对多个不同类型的分析物进行采样。 DFPSE的至少一个外表面层是限制来自下层的固体的屏障FPSE。

公开(公告)号：US09522382B2

公开(公告)日：2016-12-20

申请号：US13422485

申请日：2012-03-16

Applicant: Michael Madson

Inventor： Michael Madson

IPC: B01J20/286 ,  B01J20/32

CPC classification number: B01J20/286 ,  B01J20/3219 ,  B01J20/3274 ,  Y10T436/255 ,  G01N30/74 ,  G01N21/33

Abstract: Methods for making designed carbohydrate affinity columns and lectin columns are provided. Reducing sugar of choice is dissolved in pH 6.0 acetate buffer and injected onto a primary amino group functionality column. The column is washed with pH 6.0 acetate buffer and the column effluent monitored with a variable wavelength UV detector. Lectin columns are made by injecting appropriate lectin to the designed carbohydrate and the lectin binds to this scaffold. The lectin column is formed.

Abstract translation: 提供了制备设计的碳水化合物亲和柱和凝集素柱的方法。 将选择的还原糖溶解在pH6.0乙酸盐缓冲液中并注入到伯氨基官能团上。 将柱用pH 6.0乙酸盐缓冲液洗涤，并用可变波长UV检测器监测柱流出液。 凝胶柱是通过将合适的凝集素注入设计的碳水化合物而制成的，并且凝集素与该支架结合。 凝集素柱形成。

公开(公告)号：US09505850B2

公开(公告)日：2016-11-29

申请号：US14404479

申请日：2013-05-29

Applicant: NATIONAL UNIVERSITY CORPORATION KUMAMOTO UNIVERSITY ,  JNC CORPORATION

Inventor： Masayo Sakata ,  Masami Todokoro

IPC: C08B37/16 ,  B01J20/30 ,  C08L5/16 ,  C08G18/64 ,  B01J20/26 ,  C07H1/06 ,  C07H21/02 ,  C07H21/04 ,  B01J20/286 ,  B01J20/32

CPC classification number: B01J20/267 ,  B01J20/286 ,  B01J20/30 ,  B01J20/3042 ,  B01J20/3085 ,  B01J20/3204 ,  B01J20/321 ,  B01J20/3212 ,  B01J20/3274 ,  B01J20/3282 ,  B01J2220/52 ,  C07H1/06 ,  C07H21/02 ,  C07H21/04 ,  C08B37/0012 ,  C08B37/0015 ,  C08G18/6484 ,  C08L5/16

Abstract: A means for selectively removing ET under coexistence of a substance showing a negative charge, such as nucleic acid is described. Endotoxin is selectively removed by bringing a polymer obtained by crosslinking cyclodextrin with an isocyanate-based crosslinking agent in contact with a solution containing endotoxin and the substance showing the negative charge such as nucleic acid.

Abstract translation: 描述了在显示负电荷的物质如核酸共存下选择性除去ET的方法。 通过将通过环糊精交联而得到的聚合物与异氰酸酯基交联剂与含有内毒素的溶液和显示负电荷的物质如核酸接触来选择性地除去内毒素。

公开(公告)号：US09370735B2

公开(公告)日：2016-06-21

申请号：US14426867

申请日：2013-09-23

Applicant: Dow Global Technologies LLC

Inventor： Deepak Akolekar ,  Victor J. Sussman ,  M J. Yanjarappa ,  Phani Kiran Bollapragada ,  Scott T. Matteucci ,  Peter N. Nickias

IPC: B01D53/22 ,  B01D61/38 ,  B01J20/283 ,  B01J20/286 ,  B01D19/00 ,  C02F1/44 ,  B01J20/16 ,  B01J20/10 ,  B01J20/32 ,  B01D71/02

CPC classification number: B01D19/0031 ,  B01D53/228 ,  B01D61/38 ,  B01D69/10 ,  B01D69/142 ,  B01D71/027 ,  B01D71/028 ,  B01D2323/36 ,  B01D2325/18 ,  B01J20/10 ,  B01J20/103 ,  B01J20/16 ,  B01J20/28057 ,  B01J20/28069 ,  B01J20/28078 ,  B01J20/3204 ,  B01J20/3236 ,  B01J20/3242 ,  B01J20/3263 ,  B01J20/3285 ,  B01J20/3287 ,  C01B33/12 ,  C02F1/44

Abstract: A composition comprising a mesoporous silica having grafted therewith an ionic liquid to form a mesoporous silica composition offers desirable levels of functionality, sorption, specific surface functionalization, and selectivity for polar gas/non-polar gas and olefin/paraffin separations. One particular embodiment employs silylated 3,3′-(2,2-bis(hydroxymethyl)propane-1,3-diyl)bis(1-methyl-1H-imidazol-3-ium)bis-((trifluoromethyl-sulfonyl)amide as the ionic liquid. The mesoporous silica composition may be configured as, for example, a membrane.

Abstract translation: 包含与其接枝离子液体以形成介孔二氧化硅组合物的介孔二氧化硅的组合物为极性气体/非极性气体和烯烃/石蜡分离提供所需水平的官能度，吸附，比表面官能化和选择性。 一个具体的实施方案使用甲硅烷基化3,3' - （2,2-双（羟甲基）丙烷-1,3-二基）双（1-甲基-1H-咪唑-3-）双 - （（三氟甲基磺酰基）酰胺 作为离子液体，介孔二氧化硅组合物可以构成为例如膜。

公开(公告)号：US20160158728A1

公开(公告)日：2016-06-09

申请号：US15040771

申请日：2016-02-10

Applicant: BRIGHAM YOUNG UNIVERSITY

Inventor： Matthew R. Linford ,  Landon A. Wiest ,  David Jensen

IPC: B01J20/32 ,  B01J20/286 ,  B01D15/20 ,  G01N1/40

CPC classification number: B01J20/3248 ,  B01D15/206 ,  B01D15/34 ,  B01D15/36 ,  B01D15/361 ,  B01D15/3804 ,  B01J20/286 ,  B01J20/287 ,  B01J20/3204 ,  B01J20/3244 ,  B01J20/3246 ,  B01J20/3251 ,  B01J20/3253 ,  G01N1/405 ,  Y10T428/2991

Abstract: Modified diamond particles for use in chromatography with a desired functional group at the diamond surface, formed from reaction of hydroxyl groups at diamond surfaces with a reactive molecule.