公开(公告)号：US20250069787A1

公开(公告)日：2025-02-27

申请号：US18713584

申请日：2022-11-23

Applicant: Roche Diagnostics Operations, Inc.

Inventor： Martin Eduardo Silvestre ,  Sabine Eisold ,  Dieter Heindl ,  Kristina Peters

IPC: H01F1/33 ,  H01F1/00 ,  H01F1/34

Abstract: In a first aspect, the invention relates to a method for preparing a magnetic bead comprising at least one magnetic particle (M) and a silica coating, wherein the silica coating comprises at least two silica layers, the method comprising steps (a) to (d), wherein at least one of steps (b), (c) and (d) is/are done under sonication, wherein sonication is done with an amplitude (peak-to-peak) in the range of from 50 to 250 μm. A second aspect of the invention is related to a magnetic bead comprising (i) at least one magnetic particle (M) and (ii) a silica coating, wherein the silica coating comprises at least two silica layers; wherein the magnetic bead is stable against 7.5 M hydrochlorid acid and has a metal (cation) leaching rate in 7.5 M hydrochloric acid in the range of from 0.1 to 10%, wherein the metal (cation) leaching is determined according to a complex formation of Fe2+ with bathophenanthroline according to Reference Example 8.2. In a third aspect, the invention relates to a functionalized magnetic bead comprising at least one, magnetic bead according to the second aspect, wherein an outer silica layer is functionalized with at least one group selected from the group consisting of amino group, azide group, alkyne group, carboxyl group, thiol group, epoxy group, aryl group and alkyl group. A fourth aspect of the invention is directed to a process for functionalizing a magnetic bead according to the second aspect comprising at least one magnetic particle (M) and a silica coating, wherein the silica coating comprises at least two silica layers. A fifth aspect of the invention is related to a method for preparing magnetic Fe3O4 supra particles and a sixth aspect relates to the use of a magnetic bead according to the second aspect or of a functionalized according to the fourth aspect for immobilization of acid stable biocatalysts or for solid-phase organic synthesis using acid-stable linker.

公开(公告)号：US12203905B2

公开(公告)日：2025-01-21

申请号：US16438775

申请日：2019-06-12

Applicant: Roche Diagnostics Operations, Inc.

Inventor： Dieter Heindl ,  Uwe Kobold ,  Christoph Seidel ,  Martin Rempt

IPC: G01N30/08 ,  G01N30/02 ,  G01N30/06 ,  G01N30/28 ,  G01N30/46 ,  G01N30/72 ,  G01N33/58 ,  G01N33/68 ,  B01D15/34 ,  B01D59/44 ,  H01J49/06 ,  H01J49/10

Abstract: A method for identifying a reagent during a process in an analysis system is disclosed. The analysis system comprises a liquid chromatograph and a mass spectrometer. The method comprises providing a reagent, adding at least one chemical substance to the reagent with a concentration being below a detection level of the mass spectrometer, enriching the chemical substance within the liquid chromatograph to a concentration above the detection level of the mass spectrometer, processing the reagent together with the enriched chemical substance by means of the analysis system, and identifying the reagent based on a detection of a substance detection signal of the mass spectrometer representing the chemical substance.

公开(公告)号：US20240092742A1

公开(公告)日：2024-03-21

申请号：US18500149

申请日：2023-11-02

Applicant: Roche Diagnostics Operations, Inc.

Inventor： Dieter Heindl ,  Hans-Peter Josel ,  Uwe Kobold ,  Christoph Seidel ,  Martin Rempt ,  Andreas Leinenbach ,  Giuseppe Prencipe ,  Silvia Baecher ,  Simon Ferdinand Loibl ,  Anna-Skrollan Geiermann ,  Jelena Milic ,  Nicole Pirkl

IPC: C07D249/06 ,  C07D203/04 ,  C07D249/04 ,  C07D257/04 ,  C07D401/04 ,  C07D401/14 ,  C07D403/04 ,  C07D403/06 ,  C07D471/04

CPC classification number: C07D249/06 ,  C07D203/04 ,  C07D249/04 ,  C07D257/04 ,  C07D401/04 ,  C07D401/14 ,  C07D403/04 ,  C07D403/06 ,  C07D471/04 ,  G01N30/72

Abstract: The present invention relates to reagents which are suitable to be used in mass spectrometry as well as methods of mass spectrometric determination of analyte molecules using said reagents.

公开(公告)号：US20230333095A1

公开(公告)日：2023-10-19

申请号：US18339799

申请日：2023-06-22

Applicant: Roche Diagnostics Operations, Inc.

Inventor： Maksim Fomin ,  Dieter Heindl ,  Lars Hillringhaus ,  Hannes Kuchelmeister ,  Mohamed Yosry Hassan Mohamed ,  Tobias Oelschlaegel ,  Sona Simonyiova

IPC: G01N33/533 ,  G01N33/68 ,  C07F15/02

CPC classification number: G01N33/533 ,  G01N33/6887 ,  C07F15/025 ,  G01N2800/325

Abstract: The present invention relates to a method for determining at least one analyte of interest. The present invention further relates to a kit, a complex, a method to synthesize a complex and the use thereof for detecting the analyte of interest in the sample.

公开(公告)号：US20210188788A1

公开(公告)日：2021-06-24

申请号：US17154501

申请日：2021-01-21

Applicant: Roche Diagnostics Operations, Inc.

Inventor： Dieter Heindl ,  Hans-Peter Josel ,  Uwe Kobold ,  Christoph Seidel ,  Martin Rempt ,  Andreas Leinenbach ,  Giuseppe Prencipe ,  Silvia Baecher ,  Simon Ferdinand Loibl ,  Anna-Skrollan Geiermann ,  Jelena Milic ,  Robert Hahn ,  Nicole Pirkl

IPC: C07D257/04 ,  C07D249/06 ,  H01J49/00

Abstract: The present invention relates to reagents which are suitable to be used in mass spectrometry as well as methods of mass spectrometric determination of analyte molecules using said reagents.

公开(公告)号：US20210102944A1

公开(公告)日：2021-04-08

申请号：US17115069

申请日：2020-12-08

Applicant: Roche Diagnostics Operations, Inc.

Inventor： Hans-Peter Josel ,  Dieter Heindl ,  Thomas Froehlich ,  Stefanie Froehner

IPC: G01N33/569 ,  C12N11/06 ,  G01N33/50 ,  G01N33/543 ,  C12N5/00 ,  C12N11/14 ,  C12N11/08

Abstract: The present invention relates to a method of immobilizing a cell on a support, the method comprising a) providing a compound or salt thereof comprising, preferably consisting of, one or more hydrophobic domains attached to a hydrophilic domain, wherein the one or more hydrophobic domains are covalently bound to said hydrophilic domain, and wherein the one or more hydrophobic domains each comprise a linear lipid, a steroid or a hydrophobic vitamin, and wherein the hydrophilic domain comprises a polyethylene glycol (PEG) moiety, and wherein the compound comprises a linking group; b) contacting a cell with the compound under conditions allowing the interaction of the compound with the membrane of the cell, thereby immobilizing the linking group on the surface of the cell; and c) contacting the linking group immobilized on the cell with a support capable of binding the linking group, thereby immobilizing the cell on the support.

公开(公告)号：US10890585B2

公开(公告)日：2021-01-12

申请号：US15186581

申请日：2016-06-20

Applicant: Roche Diagnostics Operations, Inc.

Inventor： Hans-Peter Josel ,  Dieter Heindl ,  Thomas Froehlich ,  Stefanie Froehner

IPC: C12N11/06 ,  C12N11/08 ,  G01N33/569 ,  G01N33/50 ,  G01N33/543 ,  C12N5/00 ,  C12N11/14

Abstract: The present invention relates to a method of immobilizing a cell on a support, the method comprising a) providing a compound or salt thereof comprising, preferably consisting of, one or more hydrophobic domains attached to a hydrophilic domain, wherein the one or more hydrophobic domains are covalently bound to said hydrophilic domain, and wherein the one or more hydrophobic domains each comprise a linear lipid, a steroid or a hydrophobic vitamin, and wherein the hydrophilic domain comprises a polyethylene glycol (PEG) moiety, and wherein the compound comprises a linking group; b) contacting a cell with the compound under conditions allowing the interaction of the compound with the membrane of the cell, thereby immobilizing the linking group on the surface of the cell; and c) contacting the linking group immobilized on the cell with a support capable of binding the linking group, thereby immobilizing the cell on the support.

公开(公告)号：US20170363624A1

公开(公告)日：2017-12-21

申请号：US15186581

申请日：2016-06-20

Applicant: Roche Diagnostics Operations, Inc.

Inventor： Hans-Peter Josel ,  Dieter Heindl ,  Thomas Froehlich ,  Stefanie Froehner

IPC: G01N33/569 ,  C12N11/08 ,  C12N11/06

CPC classification number: G01N33/56972 ,  C12N5/0068 ,  C12N11/06 ,  C12N11/08 ,  C12N11/14 ,  C12N2503/00 ,  C12N2533/20 ,  C12N2533/30 ,  G01N33/5005 ,  G01N33/54353 ,  G01N33/56966

Abstract: The present invention relates to a method of immobilizing a cell on a support, the method comprising a) providing a compound or salt thereof comprising, preferably consisting of, one or more hydrophobic domains attached to a hydrophilic domain, wherein the one or more hydrophobic domains are covalently bound to said hydrophilic domain, and wherein the one or more hydrophobic domains each comprise a linear lipid, a steroid or a hydrophobic vitamin, and wherein the hydrophilic domain comprises a polyethylene glycol (PEG) moiety, and wherein the compound comprises a linking group; b) contacting a cell with the compound under conditions allowing the interaction of the compound with the membrane of the cell, thereby immobilizing the linking group on the surface of the cell; and c) contacting the linking group immobilized on the cell with a support capable of binding the linking group, thereby immobilizing the cell on the support.

公开(公告)号：US20160090398A1

公开(公告)日：2016-03-31

申请号：US14959280

申请日：2015-12-04

Applicant: Roche Diagnostics Operations, Inc.

Inventor： Dieter Heindl ,  Joachim Hoenes ,  Carina Horn ,  Thomas Meier ,  Wolfgang Petrich

IPC: C07H19/207 ,  G01N21/64 ,  C12Q1/26

CPC classification number: C07H19/207 ,  C12Q1/26 ,  G01N21/6428 ,  G01N33/533 ,  G01N33/535 ,  G01N33/542 ,  G01N33/5735 ,  G01N33/581 ,  G01N33/582 ,  G01N2201/062 ,  G01N2333/90248

Abstract: The invention also relates to compounds, which are useful for intra-molecular fluorescence resonance energy transfer (FRET), comprising the oxidized form of a carbaNADH-based first fluorophore and a second fluorophore that is excitable at a wave-length of between 445 to 540 nm and that has an emission maximum of greater than 560 nm, and methods, kits and compositions related thereto.

Abstract translation: 本发明还涉及可用于分子内荧光共振能量转移（FRET）的化合物，其包含基于carbaNADH的第一荧光团的氧化形式和可在波长445至540之间激发的第二荧光团 nm，其发射最大值大于560nm，以及与其相关的方法，试剂盒和组合物。

公开(公告)号：US08981070B2

公开(公告)日：2015-03-17

申请号：US13866412

申请日：2013-04-19

Applicant: Roche Diagnostics Operations, Inc.

Inventor： Simone Budow ,  Ping Ding ,  Dieter Heindl ,  Alfons Nichtl ,  Frank Seela

IPC: C07H21/02 ,  C07H21/00 ,  C12Q1/68

CPC classification number: C12Q1/6876 ,  C07H21/02

Abstract: An oligonucleotide-solid phase conjugate, wherein the solid phase is thiophilic and the oligonucleotide comprises at least one thiooxonucleobase according to Formula I, wherein X is CH or N, R1 is H or NH2, --- indicates a covalent bond, and said oligonucleotide is bound to said solid phase via the sulfur atom of said thiooxonucleotide. Also disclosed are methods for producing such conjugate as well as various uses for such oligonucleotide metal conjugate.

Abstract translation: 一种寡核苷酸 - 固相缀合物，其中所述固相是亲核的，并且所述寡核苷酸包含至少一种根据式I的硫氧基核碱基，其中X为CH或N，R 1为H或NH 2，表示共价键，所述寡核苷酸 通过所述硫代核苷酸的硫原子与所述固相结合。 还公开了用于生产这种缀合物的方法以及这种寡核苷酸金属缀合物的各种用途。