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公开(公告)号：US20190300588A1
公开(公告)日：2019-10-03
申请号：US16371932
申请日：2019-04-01
Applicant: SPIBER INC.
Inventor： Kaori SEKIYAMA , Mizuki ISHIKAWA , Shinya MURATA
Abstract: A film of the present invention contains a polypeptide derived from spider silk proteins. The decomposition temperature of the film is 240 to 260° C. The film absorbs ultraviolet light having a wavelength of 200 to 300 nm and has a light transmittance of 85% or more at a wavelength of 400 to 780 nm. The film is transparent and colorless in a visible light region. A method for producing a film of the present invention includes: dissolving a polypeptide derived from spider silk proteins in a dimethyl sulfoxide solvent to prepare a dope; and cast-molding the dope on a surface of a base. Thus, the present invention provides a spider silk protein film that can be formed easily and has favorable stretchability, and a method for producing the same.
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62.发明申请公开(公告)号：US20190228449A1
公开(公告)日：2019-07-25
申请号：US16372508
申请日：2019-04-02
Applicant: SPIBER INC.
Inventor： Kazuhide SEKIYAMA , Ryo HATTORI , Goshi UHIRA
Abstract: In a garment production management apparatus, a garment information acquisition part acquires garment information to specify a garment an orderer has selected. A body shape information acquisition part acquires body shape information of a wearer who wears the garment. A production information acquisition part acquires the production information of the garment on the basis of the garment information and the body shape information. A production information output part outputs the production information to a seamless knitting machine. Here, the production information acquisition part may select production information from among a plurality of different pieces of production information associated with the garment information or modify the production information that serves as a base of the garment associated with the garment information on the basis of the body shape information.
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公开(公告)号：US20190194710A1
公开(公告)日：2019-06-27
申请号：US16322518
申请日：2017-08-02
Applicant: Spiber Inc.
Inventor： Kenji Kurachi , Mihoko Kinoshita
CPC classification number: C12P21/00 , C12N1/00 , C12N5/0025 , C12N15/635 , C12P21/02
Abstract: The invention relates to a method for production of a recombinant protein using recombinant cells that express the recombinant protein under the control of an inducible promoter, the method including continuous culturing with addition of fresh culture medium to a portion of the culture solution in which the recombinant cells have been grown, wherein the recombinant cells whose expression of the recombinant protein has been induced are not reused.
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公开(公告)号：US20190135881A1
公开(公告)日：2019-05-09
申请号：US16096824
申请日：2017-04-28
Applicant: Spiber Inc. , Kojima Industries Corporation
Inventor： Keisuke Morita , Junichi Sugahara
IPC: C07K14/435 , D01F4/02
Abstract: Provided is a modified fibroin, including a domain sequence represented by Formula 1: [(A)n motif-REP]m, in which the domain sequence has an amino acid sequence having a reduced content of glycine residues equivalent to an amino acid sequence in which, at least, one or a plurality of the glycine residues in REP is substituted with another amino acid residue, as compared to naturally occurring fibroin:[In Formula 1, (A)n motif represents an amino acid sequence consisting of 4 to 20 amino acid residues and the number of alanine residues relative to the total number of amino acid residues in the (A)n motif is 83% or more, REP represents an amino acid sequence consisting of 10 to 200 amino acid residues, m represents an integer of 8 to 300, and a plurality of (A)n motifs or the like may be the same amino acid sequence or different amino acid sequences.]
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公开(公告)号：US20190040109A1
公开(公告)日：2019-02-07
申请号：US15760497
申请日：2016-09-08
Applicant: Spiber Inc. , Kojima Industries Corporation , Tekunohama Co., Ltd.
Inventor： Junichi Sugahara , Kaori Sekiyama , Ayumi Abe , Junichi Shimokata , Junichi Noba , Shinji Hirai
IPC: C07K14/435
Abstract: Provided is a molded article of a composition comprising a polypeptide, wherein the polypeptide is at least one kind selected from the group consisting of natural spider silk protein and polypeptides derived from natural spider silk protein.
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Patent Agency Ranking
公开(公告)号：US20180265555A1
公开(公告)日：2018-09-20
申请号：US15989400
申请日：2018-05-25
Applicant: AJINOMOTO CO., INC. , SPIBER INC.
Inventor： Kentaro Nakase , Yoshinori Takashima , Takeshi Nagahiko
IPC: C07K14/435 , C12N15/71 , C12P21/02
CPC classification number: C07K14/43518 , C07K2319/02 , C07K2319/034 , C07K2319/035 , C12N15/09 , C12N15/71 , C12P21/02 , D01F4/02
Abstract: A method for producing a fibroin-like protein is provided. A fibroin-like protein is produced by a method of culturing Escherichia coli having a gene encoding the fibroin-like protein in a medium, inducing expression of the gene encoding the fibroin-like protein, and collecting the fibroin-like protein, wherein accumulation of an organic acid at the time of inducing the expression is reduced, and wherein the gene is expressed under control of a tryptophan promoter.
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公开(公告)号：US20150376247A1
公开(公告)日：2015-12-31
申请号：US14764472
申请日：2014-04-18
Applicant: SPIBER INC.
Inventor： Toshiaki OSAWA , Keisuke MORITA
CPC classification number: C07K14/43518 , A61K8/0279 , A61K8/42 , A61K8/46 , A61K8/64 , A61K9/5169 , A61K47/42 , A61K2800/412 , A61K2800/56 , A61K2800/84 , A61Q19/00 , C08J3/05 , C08J3/07 , C08J3/12 , C08J2389/00 , C08L89/00 , C09D7/65 , C09D7/68
Abstract: Polypeptide particles of the present invention are particles of a polypeptide derived from spider silk proteins, and have an average particle size of 1000 nm or less. A method for producing polypeptide particles of the present invention includes: a solution production step in which the polypeptide is dissolved in at least one solvent selected from the group consisting of DMSO, DMF, and these with an inorganic salt, so as to obtain a solution of the polypeptide; a step in which the solution produced in the solution production step is substituted with a water-soluble solvent so as to obtain an aqueous solution of the polypeptide; and a step in which the aqueous solution of the polypeptide is dried. Thereby, the present invention provides polypeptide particles suitable for application to a living body and capable of being applied to cosmetics, etc., while identifying the properties of the polypeptide particles, and a method for producing the same.
Abstract translation: 本发明的多肽粒子是来自蜘蛛丝蛋白质的多肽的粒子，平均粒径为1000nm以下。 本发明的多肽粒子的制造方法包括：溶液制备步骤，其中将多肽溶解于至少一种选自DMSO，DMF的溶剂中，并用无机盐溶解，得到溶液 的多肽; 在溶液制备工序中产生的溶液被水溶性溶剂代替以获得多肽的水溶液的步骤; 以及将多肽的水溶液干燥的步骤。 因此，本发明提供适用于生物体并能够应用于化妆品等的多肽颗粒，同时鉴定多肽颗粒的性质及其制备方法。
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公开(公告)号：US12195883B2
公开(公告)日：2025-01-14
申请号：US17279081
申请日：2019-09-27
Applicant: Spiber Inc.
Inventor： Yunosuke Abe , Masato Matsuo , Shota Togashi
Abstract: The present invention provides a modified fibroin fiber having a shrinkage history of being irreversibly shrunk after spinning, the modified fibroin fiber containing modified fibroin, wherein a fiber diameter of a raw material fiber before being irreversibly shrunk exceeds 25 μm.
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公开(公告)号：US20240383943A1
公开(公告)日：2024-11-21
申请号：US18704414
申请日：2022-11-02
Applicant: Spiber Inc.
Inventor： Kazuki Sakata , Seiji Shirakawa , Nao Kitahara , Takahiro Niwa
Abstract: The present invention provides a β-ketoester of a protein containing an amino acid having a hydroxyl group, wherein the hydroxyl group forms an ester bond with a group represented by Formula (1) wherein R represents a C1-6 alkyl group or an optionally substituted aryl group.
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公开(公告)号：US11981088B2
公开(公告)日：2024-05-14
申请号：US17043820
申请日：2019-04-01
Applicant: Spiber Inc.
Inventor： Kazuki Sakata , Kentaro Takahashi
CPC classification number: B29C65/1403 , C07K14/43518 , D01F1/02 , D01F4/02 , B29K2027/12 , B29K2083/00 , B29K2089/00 , B29K2995/0069
Abstract: Disclosed is a molded article including a modified fibroin and a water resistance-imparting material. The modified fibroin and the water resistance-imparting material may be covalently bonded.
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