公开(公告)号：US20190157060A1

公开(公告)日：2019-05-23

申请号：US16198667

申请日：2018-11-21

Applicant: Labcyte, Inc. ,  DH Technologies Development Pte. Ltd.

Inventor： Sammy Datwani ,  Donald W. Arnold ,  Lucien P. Ghislain ,  Chang Liu ,  Thomas Covey

IPC: H01J49/04 ,  H01J49/00

CPC classification number: H01J49/0454 ,  B01L3/0268 ,  B01L2400/0439 ,  H01J49/0031 ,  H01J49/0404 ,  H01J49/165

Abstract: A system and method are provided for loading a sample into an analytical instrument using acoustic droplet ejection (“ADE”) in combination with a continuous flow sampling probe. An acoustic droplet ejector is used to eject small droplets of a fluid sample containing an analyte into the sampling tip of a continuous flow sampling probe, where the acoustically ejected droplet combines with a continuous, circulating flow stream of solvent within the flow probe. Fluid circulation within the probe transports the sample through a sample transport capillary to an outlet that directs the analyte away from the probe to an analytical instrument, e.g., a device that detects the presence, concentration quantity, and/or identity of the analyte. When the analytical instrument is a mass spectrometer or other type of device requiring the analyte to be in ionized form, the exiting droplets pass through an ionization region, e.g., an electrospray ion source, prior to entering the mass spectrometer or other analytical instrument. The method employs active flow control and enables real-time kinetic measurements.

公开(公告)号：US10156499B1

公开(公告)日：2018-12-18

申请号：US14041156

申请日：2013-09-30

Applicant: Labcyte Inc.

Inventor： Richard G. Stearns

IPC: G01N1/28 ,  G01N1/10

Abstract: Focused acoustic radiation, referred to as tonebursts, are applied to a volume of liquid to generate a set of droplets. The droplets generated are substantially smaller in scale than the focal spot size of the acoustic beam (e.g., the frequency at which the acoustic transducer operates). Further, the droplets have trajectories that are substantially in the direction of the acoustic beam propagation direction. In one embodiment, a first toneburst is applied to temporarily raise a protuberance on a free surface of the fluid. After the protuberance has reached a certain state, a second toneburst is applied to the protuberance to break it into very small droplets. In one embodiment, the state of the protuberance at which the second toneburst is supplied is the time period shortly after the protuberance reaches its maximum height but before the protuberance recedes back into the volume of fluid.

公开(公告)号：US20180304251A1

公开(公告)日：2018-10-25

申请号：US15972019

申请日：2018-05-04

Applicant: LABCYTE INC.

Inventor： Richard N. Ellson ,  Joseph D. Olechno ,  Robert G. Kuimelis

IPC: B01L3/00 ,  G01N35/10 ,  B01L3/02

CPC classification number: B01L3/50 ,  B01L3/0268 ,  B01L3/50215 ,  B01L2200/0684 ,  B01L2400/0415 ,  B01L2400/0436 ,  G01N35/10 ,  G01N2035/1041

Abstract: Sample container for holding and transferring a liquid sample and method thereof. The sample container includes an inlet configured to allow a liquid sample to enter a sample container, and an outlet configured to allow one or more droplets of the liquid sample to exit the sample container by one or more acoustic ejections respectively. The inlet and the outlet are in different locations.

公开(公告)号：US09610577B2

公开(公告)日：2017-04-04

申请号：US14884237

申请日：2015-10-15

Applicant: LABCYTE INC.

Inventor： Richard N. Ellson

IPC: B01L3/00 ,  B01L3/02 ,  G01N1/10 ,  G01N1/02 ,  G01N1/00

CPC classification number: B01L3/502 ,  B01L3/0268 ,  B01L2200/0642 ,  B01L2200/142 ,  B01L2300/046 ,  B01L2300/0829 ,  B01L2300/0832 ,  B01L2300/0851 ,  B01L2400/0436 ,  B01L2400/0439 ,  G01N1/00 ,  G01N1/02 ,  G01N1/10

Abstract: In an aspect of this invention, a closure for a well plate is provided which has a reservoir. The closure has openings through which acoustic ejection of fluid droplets can take place without removing the closure. The reservoirs in the closure may help to maintain acceptable levels of solvent in the wells of the well plate despite the evaporation which may occur during the course of ejection.

公开(公告)号：US20150196904A1

公开(公告)日：2015-07-16

申请号：US14595063

申请日：2015-01-12

Applicant: LABCYTE INC.

Inventor： Richard N. ELLSON

IPC: B01L3/00 ,  B01L3/02

CPC classification number: B01L3/50857 ,  B01J2219/00313 ,  B01J2219/00319 ,  B01J2219/00362 ,  B01L3/0244 ,  B01L3/0268 ,  B01L3/50855 ,  B01L2400/0436

Abstract: Provided herein is generally tubular container, preferably including a plurality of reservoirs defined therein. The container can be adapted for acoustic ejection of a fluid disposed within at least one of the reservoirs of the plurality of reservoirs. Alternatively, the container can be adapted for extraction of a fluid disposed within at least one of the reservoirs of the plurality of reservoirs using a non-acoustic liquid handling method.

Abstract translation: 本文提供的是通常为管状容器，优选地包括限定在其中的多个储存器。 容器可以适于声音喷射设置在多个储存器的至少一个储存器中的流体。 或者，容器可以适于使用非声学液体处理方法来提取设置在多个储存器的至少一个储存器内的流体。

公开(公告)号：US20130193243A1

公开(公告)日：2013-08-01

申请号：US13752077

申请日：2013-01-28

Applicant: Labcyte Inc.

Inventor： Richard G. Stearns ,  Stephen J. Hinkson

IPC: B05B5/00

CPC classification number: B05B5/005 ,  B01L3/0268 ,  B01L2300/0819 ,  B01L2300/0829 ,  B01L2400/027 ,  B01L2400/0415 ,  B01L2400/0436 ,  B01L2400/0487 ,  B05B17/06 ,  G01N35/1009 ,  G01N35/1074 ,  G01N2035/1034 ,  Y10T436/2575

Abstract: A system for fluid transport is provided where a quantity of fluid is held in a reservoir. A droplet generator is employed to generate droplets from the fluid, for example a nozzle-based system or a nozzleless system such as an acoustic ejection system. A generated droplet has a trajectory whereby it arrives at a target. A circuit is used to modify one or more characteristics of the generated droplet in a way which increases the likelihood that the droplet will not splash or bounce when it arrives at the target. The circuit may in different embodiments control the speed of the droplet or the Weber number of the droplet. The circuit may create an electric field in an area of space where the droplet passes. The circuit may charge the droplet by causing it to contact ions.

Abstract translation: 提供一种用于流体输送的系统，其中将一定量的流体保持在储存器中。 液滴发生器用于从流体产生液滴，例如基于喷嘴的系统或无喷射系统，例如声弹射系统。 产生的液滴具有到达目标的轨迹。 电路用于以增加液滴在到达目标时不会飞溅或反弹的可能性的方式来修改产生的液滴的一个或多个特性。 电路可以在不同的实施例中控制液滴的速度或微波的韦伯数。 电路可以在液滴通过的空间区域中产生电场。 该电路可通过使其与离子接触来对液滴进行充电。