公开(公告)号：US20150177150A1

公开(公告)日：2015-06-25

申请号：US14543867

申请日：2014-11-17

Applicant: Quantum-Si Incorporated

Inventor： Jonathan M. Rothberg ,  Ali Kabiri ,  Jason W. Sickler ,  Brett J. Gyarfas ,  Jeremy Lackey ,  Gerard Schmid ,  Benjamin Cipriany ,  Jack Jewell ,  Lawrence West ,  Michael Ferrigno ,  Paul E. Glenn ,  Adam E. Cohen ,  Anthony Bellofiore

IPC: G01N21/64

CPC classification number: G01N21/6486 ,  B01L3/5085 ,  B01L2200/12 ,  B01L2300/0829 ,  B01L2300/0887 ,  B01L2300/0893 ,  B01L2300/168 ,  C12Q1/6869 ,  C12Q1/6874 ,  C12Q2521/101 ,  C12Q2537/157 ,  C12Q2563/107 ,  C12Q2565/607 ,  G01N21/64 ,  G01N21/645 ,  G01N21/6452 ,  G01N21/648 ,  G01N21/7743 ,  G01N21/7746 ,  G01N2021/6419 ,  G01N2021/6441 ,  G01N2021/6478 ,  G01N2201/02 ,  G01N2201/06113 ,  G01N2201/0612 ,  G01N2201/062 ,  G01N2201/068 ,  G01N2201/125 ,  Y10T29/49016 ,  C12Q2525/101

Abstract: Apparatus and methods for analyzing single molecule and performing nucleic acid sequencing. An apparatus can include an assay chip that includes multiple pixels with sample wells configured to receive a sample, which, when excited, emits emission energy; at least one element for directing the emission energy in a particular direction; and a light path along which the emission energy travels from the sample well toward a sensor. The apparatus also includes an instrument that interfaces with the assay chip. The instrument includes an excitation light source for exciting the sample in each sample well; a plurality of sensors corresponding the sample wells. Each sensor may detect emission energy from a sample in a respective sample well. The instrument includes at least one optical element that directs the emission energy from each sample well towards a respective sensor of the plurality of sensors.

Abstract translation: 用于分析单分子和进行核酸测序的装置和方法。 装置可以包括测定芯片，其包括多个像素，其中样品阱被配置为接收样品，当被激发时，样品阱发射发射能量; 用于在特定方向上引导发射能量的至少一个元件; 以及发光能量从样品阱向传感器行进的光路。 该装置还包括与测定芯片接口的仪器。 仪器包括用于激发每个样品井中的样品的激发光源; 多个传感器对应于样品孔。 每个传感器可以检测来自相应样品阱中的样品的发射能量。 仪器包括至少一个光学元件，其将来自每个样品的发射能量引向多个传感器中的相应传感器。

公开(公告)号：US20150141268A1

公开(公告)日：2015-05-21

申请号：US14543888

申请日：2014-11-17

Applicant: Quantum-Si Incorporated

Inventor： Jonathan M. Rothberg ,  Ali Kabiri ,  Jason W. Sickler ,  Brett J. Gyarfas ,  Jeremy Lackey ,  Gerard Schmid

IPC: C12Q1/68 ,  G01N21/64

CPC classification number: G01N21/6486 ,  B01L3/5085 ,  B01L2200/12 ,  B01L2300/0829 ,  B01L2300/0887 ,  B01L2300/0893 ,  B01L2300/168 ,  C12Q1/6869 ,  C12Q1/6874 ,  C12Q2521/101 ,  C12Q2537/157 ,  C12Q2563/107 ,  C12Q2565/607 ,  G01N21/64 ,  G01N21/645 ,  G01N21/6452 ,  G01N21/648 ,  G01N21/7743 ,  G01N21/7746 ,  G01N2021/6419 ,  G01N2021/6441 ,  G01N2021/6478 ,  G01N2201/02 ,  G01N2201/06113 ,  G01N2201/0612 ,  G01N2201/062 ,  G01N2201/068 ,  G01N2201/125 ,  Y10T29/49016 ,  C12Q2525/101

Abstract: An active-source-pixel, integrated device capable of performing biomolecule detection and/or analysis, such as single-molecule nucleic acid sequencing, is described. An active pixel of the integrated device includes a sample well into which a sample to be analyzed may diffuse, an excitation source for providing excitation energy to the sample well, and a sensor configured to detect emission from the sample. The sensor may comprise two or more segments that produce a set of signals that are analyzed to differentiate between and identify tags that are attached to, or associated with, the sample. Tag differentiation may be spectral and/or temporal based. Identification of the tags may be used to detect, analyze, and/or sequence the biomolecule.

Abstract translation: 描述了能够进行生物分子检测和/或分析的活性源像素集成器件，例如单分子核酸测序。 集成器件的有源像素包括样本阱，待分析的样本可以扩散到其中，用于向样本阱提供激发能量的激发源以及被配置为检测来自样本的发射的传感器。 传感器可以包括产生一组信号的两个或更多个片段，所述一组信号被分析以区分和识别附加到样本或与样本相关联的标签。 标签分化可以是基于频谱和/或时间的。 标签的识别可用于检测，分析和/或序列生物分子。

公开(公告)号：US20150141267A1

公开(公告)日：2015-05-21

申请号：US14543865

申请日：2014-11-17

Applicant: Quantum-Si Incorporated

Inventor： Jonathan M. Rothberg ,  Ali Kabiri ,  Jason W. Sickler ,  Brett J. Gyarfas ,  Jeremy Lackey ,  Gerard Schmid ,  Benjamin Cipriany ,  Jack Jewell ,  Lawrence West ,  Michael Ferrigno ,  Paul E. Glenn ,  Anthony Bellofiore

IPC: C12Q1/68 ,  G01N21/64

CPC classification number: G01N21/6486 ,  B01L3/5085 ,  B01L2200/12 ,  B01L2300/0829 ,  B01L2300/0887 ,  B01L2300/0893 ,  B01L2300/168 ,  C12Q1/6869 ,  C12Q1/6874 ,  C12Q2521/101 ,  C12Q2537/157 ,  C12Q2563/107 ,  C12Q2565/607 ,  G01N21/64 ,  G01N21/645 ,  G01N21/6452 ,  G01N21/648 ,  G01N21/7743 ,  G01N21/7746 ,  G01N2021/6419 ,  G01N2021/6441 ,  G01N2021/6478 ,  G01N2201/02 ,  G01N2201/06113 ,  G01N2201/0612 ,  G01N2201/062 ,  G01N2201/068 ,  G01N2201/125 ,  Y10T29/49016 ,  C12Q2525/101

Abstract: Apparatus and methods for analyzing single molecule and performing nucleic acid sequencing. An integrated device includes multiple pixels with sample wells configured to receive a sample, which, when excited, emits radiation; at least one element for directing the emission radiation in a particular direction; and a light path along which the emission radiation travels from the sample well toward a sensor. The apparatus also includes an instrument that interfaces with the integrated device. Each sensor may detect emission radiation from a sample in a respective sample well. The instrument includes an excitation light source for exciting the sample in each sample well.

Abstract translation: 用于分析单分子和进行核酸测序的装置和方法。 集成器件包括多个像素，其中样品阱被配置为接收样品，当被激发时，样品阱发射辐射; 用于在特定方向上引导发射辐射的至少一个元件; 以及发射辐射从样品阱向传感器行进的光路。 该装置还包括与集成装置接口的仪器。 每个传感器可以检测来自相应样品阱中的样品的发射辐射。 该仪器包括用于激发每个样品孔中的样品的激发光源。

公开(公告)号：US20250130238A1

公开(公告)日：2025-04-24

申请号：US18752732

申请日：2024-06-24

Applicant: Quantum-Si Incorporated

Inventor： Brian Reed ,  Jeremy Lackey ,  Haidong Huang

IPC: G01N33/58 ,  C07K14/47 ,  C07K19/00 ,  C12Q1/6806 ,  G01N1/28 ,  G01N21/64 ,  G01N33/68 ,  G16B25/10 ,  G16B30/00 ,  G16B40/00 ,  G16B40/10 ,  G16B50/00 ,  G16B50/30

Abstract: Aspects of the application provide methods of identifying and sequencing proteins, polypeptides, and amino acids, and compositions useful for the same. In some aspects, the application provides methods of obtaining data during a degradation process of a polypeptide, and outputting a sequence representative of the polypeptide. In some aspects, the application provides amino acid recognition molecules comprising a shielding element that enhances photostability in polypeptide sequencing reactions.

公开(公告)号：US20250011840A1

公开(公告)日：2025-01-09

申请号：US18611338

申请日：2024-03-20

Applicant: Quantum-Si Incorporated

Inventor： Jonathan M. Rothberg ,  Jeremy Lackey ,  Brian Reed ,  Xinghua Shi ,  Haidong Huang

IPC: C12Q1/6818 ,  C12Q1/6869 ,  C12Q1/6874 ,  G01N21/64

Abstract: Methods of sequencing molecules based on luminescence lifetimes and/or intensities are provided. In some aspects, methods of sequencing nucleic acids involve determining the luminescence lifetimes, and optionally luminescence intensities, of a series of luminescently labeled nucleotides incorporated during a nucleic acid sequencing reaction.

公开(公告)号：US12174196B2

公开(公告)日：2024-12-24

申请号：US18598736

申请日：2024-03-07

Applicant: Quantum-Si Incorporated

Inventor： Brian Reed ,  Jeremy Lackey ,  Haidong Huang

IPC: G16B40/00 ,  C07K14/47 ,  C07K19/00 ,  C12Q1/6806 ,  G01N1/28 ,  G01N21/64 ,  G01N33/58 ,  G01N33/68 ,  G16B25/10 ,  G16B40/10 ,  G16B50/30 ,  G16B30/00 ,  G16B50/00

Abstract: Aspects of the application provide methods of identifying and sequencing proteins, polypeptides, and amino acids, and compositions useful for the same. In some aspects, the application provides methods of obtaining data during a degradation process of a polypeptide, and outputting a sequence representative of the polypeptide. In some aspects, the application provides amino acid recognition molecules comprising a shielding element that enhances photostability in polypeptide sequencing reactions.

公开(公告)号：US12123834B2

公开(公告)日：2024-10-22

申请号：US16900008

申请日：2020-06-12

Applicant: Quantum-Si Incorporated

Inventor： Jonathan M. Rothberg ,  Ali Kabiri ,  Jason W. Sickler ,  Brett J. Gyarfas ,  Jeremy Lackey ,  Gerard Schmid

IPC: B01L3/00 ,  C12Q1/6869 ,  C12Q1/6874 ,  G01N21/64 ,  G01N21/77

CPC classification number: G01N21/6486 ,  B01L3/5085 ,  C12Q1/6869 ,  C12Q1/6874 ,  G01N21/64 ,  G01N21/645 ,  G01N21/6452 ,  G01N21/648 ,  G01N21/7743 ,  G01N21/7746 ,  B01L2200/12 ,  B01L2300/0829 ,  B01L2300/0887 ,  B01L2300/0893 ,  B01L2300/168 ,  C12Q2521/101 ,  C12Q2525/101 ,  C12Q2537/157 ,  C12Q2563/107 ,  C12Q2565/607 ,  G01N2021/6419 ,  G01N2021/6441 ,  G01N2021/6478 ,  G01N2201/02 ,  G01N2201/06113 ,  G01N2201/0612 ,  G01N2201/062 ,  G01N2201/068 ,  G01N2201/125 ,  Y10T29/49016 ,  C12Q1/6874 ,  C12Q2521/101 ,  C12Q2525/101 ,  C12Q2537/157 ,  C12Q2563/107 ,  C12Q2565/607

Abstract: An active-source-pixel, integrated device capable of performing biomolecule detection and/or analysis, such as single-molecule nucleic acid sequencing, is described. An active pixel of the integrated device includes a sample well into which a sample to be analyzed may diffuse, an excitation source for providing excitation energy to the sample well, and a sensor configured to detect emission from the sample. The sensor may comprise two or more segments that produce a set of signals that are analyzed to differentiate between and identify tags that are attached to, or associated with, the sample. Tag differentiation may be spectral and/or temporal based. Identification of the tags may be used to detect, analyze, and/or sequence the biomolecule.

公开(公告)号：US20240209434A1

公开(公告)日：2024-06-27

申请号：US18431313

申请日：2024-02-02

Applicant: Quantum-Si Incorporated

Inventor： Guojun Chen ,  Jeremy Lackey ,  Alexander Goryaynov ,  Gerard Schmid ,  Ali Kabiri ,  Jonathan M. Rothberg ,  Todd Rearick ,  Jonathan C. Schultz ,  Farshid Ghasemi ,  Keith G. Fife

IPC: C12Q1/6869 ,  B01L3/00 ,  C08L1/02 ,  C12Q1/6806 ,  G01N21/64 ,  G01N27/447 ,  G01N30/86 ,  G01N30/88

CPC classification number: C12Q1/6869 ,  B01L3/502761 ,  B01L3/50857 ,  C08L1/02 ,  C12Q1/6806 ,  G01N21/6428 ,  G01N21/6454 ,  G01N27/44743 ,  G01N27/44782 ,  G01N30/86 ,  B01L2200/0647 ,  B01L2300/0645 ,  B01L2300/0829 ,  B01L2300/0864 ,  B01L2400/0415 ,  B01L2400/0421 ,  G01N27/447 ,  G01N30/88

Abstract: Apparatus and techniques for electrokinetic loading of samples of interest into sub-micron-scale reaction chambers are described. Embodiments include an integrated device and related apparatus for analyzing samples in parallel. The integrated device may include at least one reaction chamber formed through a surface of the integrated device and configured to receive a sample of interest, such as a molecule of nucleic acid. The integrated device may further include electrodes patterned adjacent to the reaction chamber that produce one or more electric fields that assist loading the sample into the reaction chamber. The apparatus may further include a sample reservoir having a fluid seal with the surface of the integrated device and configured to hold a suspension containing the samples.

公开(公告)号：US12000835B2

公开(公告)日：2024-06-04

申请号：US16709052

申请日：2019-12-10

Applicant: Quantum-Si Incorporated

Inventor： Brian Reed ,  Jeremy Lackey ,  Haidong Huang

IPC: G01N33/68 ,  C07K14/47 ,  C07K19/00 ,  C12Q1/6806 ,  G01N1/28 ,  G01N21/64 ,  G01N33/58 ,  G16B25/10 ,  G16B30/00 ,  G16B40/00 ,  G16B40/10 ,  G16B50/30 ,  G16B50/00

CPC classification number: G01N33/581 ,  C07K14/47 ,  C07K19/00 ,  C12Q1/6806 ,  G01N1/28 ,  G01N21/6428 ,  G01N33/58 ,  G01N33/582 ,  G01N33/6821 ,  G01N33/6824 ,  G16B25/10 ,  G16B40/00 ,  G16B40/10 ,  G16B50/30 ,  G01N2021/6439 ,  G01N2458/00 ,  G16B30/00 ,  G16B50/00

Abstract: Aspects of the application provide methods of identifying and sequencing proteins, polypeptides, and amino acids, and compositions useful for the same. In some aspects, the application provides methods of obtaining data during a degradation process of a polypeptide, and outputting a sequence representative of the polypeptide. In some aspects, the application provides amino acid recognition molecules having a shielding element that enhances photostability in polypeptide sequencing reactions.

公开(公告)号：US11959920B2

公开(公告)日：2024-04-16

申请号：US16708989

申请日：2019-12-10

Applicant: Quantum-Si Incorporated

Inventor： Brian Reed ,  Jeremy Lackey ,  Haidong Huang

IPC: G01N33/58 ,  C07K14/47 ,  C07K19/00 ,  C12Q1/6806 ,  G01N1/28 ,  G01N21/64 ,  G01N33/68 ,  G16B25/10 ,  G16B40/00 ,  G16B40/10 ,  G16B50/30 ,  G16B30/00 ,  G16B50/00

CPC classification number: G01N33/581 ,  C07K14/47 ,  C07K19/00 ,  C12Q1/6806 ,  G01N1/28 ,  G01N21/6428 ,  G01N33/58 ,  G01N33/582 ,  G01N33/6821 ,  G01N33/6824 ,  G16B25/10 ,  G16B40/00 ,  G16B40/10 ,  G16B50/30 ,  G01N2021/6439 ,  G01N2458/00 ,  G16B30/00 ,  G16B50/00

Abstract: Aspects of the application provide methods of identifying and sequencing proteins, polypeptides, and amino acids, and compositions useful for the same. In some aspects, the application provides methods of obtaining data during a degradation process of a polypeptide, and outputting a sequence representative of the polypeptide. In some aspects, the application provides amino acid recognition molecules comprising a shielding element that enhances photostability in polypeptide sequencing reactions.